Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0085
(363 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 82 3e-17
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 77 8e-16
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 70 1e-13
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 47 1e-06
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 46 2e-06
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 0.68
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 25 2.7
SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 3.6
SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe... 25 4.8
SPAC30.02c |||RNA polymerase II elongator complex subunit|Schizo... 25 4.8
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 24 6.3
SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb... 24 6.3
SPAC27F1.05c |||aminotransferase class-III, unknown specificty|S... 24 8.3
SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 24 8.3
SPBC31E1.06 |bms1|SPBC800.01|GTP binding protein Bms1|Schizosacc... 24 8.3
SPAC13G6.14 |aps1|SPAC24B11.03|diadenosine 5',5'''-p1,p6-hexapho... 24 8.3
SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|ch... 24 8.3
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 81.8 bits (193), Expect = 3e-17
Identities = 43/113 (38%), Positives = 68/113 (60%), Gaps = 2/113 (1%)
Frame = +2
Query: 29 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 202
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 203 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEK 361
F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+
Sbjct: 67 FTEFLTM--MARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGER 117
Score = 33.5 bits (73), Expect = 0.010
Identities = 15/42 (35%), Positives = 26/42 (61%)
Frame = +2
Query: 35 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 160
D E +EAF++FD G+G I V ++ L +LG+ ++ +V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEV 123
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 77.0 bits (181), Expect = 8e-16
Identities = 41/104 (39%), Positives = 60/104 (57%)
Frame = +2
Query: 50 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQ 229
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 230 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEK 361
+ +F++G + FDKD G I ELR++L++LGEK
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEK 109
Score = 27.5 bits (58), Expect = 0.68
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 47 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 160
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 69.7 bits (163), Expect = 1e-13
Identities = 38/111 (34%), Positives = 64/111 (57%)
Frame = +2
Query: 29 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFE 208
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 209 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEK 361
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLGEK
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEK 110
Score = 35.5 bits (78), Expect = 0.003
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = +2
Query: 47 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 226
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 227 QAISK 241
+ ++K
Sbjct: 139 RIMAK 143
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 46.8 bits (106), Expect = 1e-06
Identities = 32/114 (28%), Positives = 52/114 (45%)
Frame = +2
Query: 20 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERI 199
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 200 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEK 361
+ FL ++ S ND +E FD +G I + +R LS++G++
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDR 149
Score = 29.5 bits (63), Expect = 0.17
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +2
Query: 56 EAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVK 163
EAF FD GKI ++ + DAL ++G +V+
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVE 156
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 46.0 bits (104), Expect = 2e-06
Identities = 34/112 (30%), Positives = 49/112 (43%), Gaps = 2/112 (1%)
Frame = +2
Query: 29 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 202
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 203 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGE 358
E F+ + K D + FD D G IS LR + L E
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNE 141
Score = 30.3 bits (65), Expect = 0.096
Identities = 18/65 (27%), Positives = 31/65 (47%), Gaps = 2/65 (3%)
Frame = +2
Query: 35 DQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKC--TLHLKPDERISFE 208
D L E + AF+LFD GKI + + + L +N + +++ L D I+ +
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQ 166
Query: 209 VFLPI 223
F+ I
Sbjct: 167 EFIAI 171
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.5 bits (58), Expect = 0.68
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +2
Query: 47 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 226
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 227 QAIS 238
IS
Sbjct: 73 IHIS 76
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 25.4 bits (53), Expect = 2.7
Identities = 17/55 (30%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = +2
Query: 206 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK 361
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQK 696
>SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1131
Score = 25.0 bits (52), Expect = 3.6
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +1
Query: 307 WVHLFCGTATPALY 348
WVHL C + TP +Y
Sbjct: 927 WVHLICASWTPDVY 940
>SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 727
Score = 24.6 bits (51), Expect = 4.8
Identities = 15/55 (27%), Positives = 26/55 (47%)
Frame = +2
Query: 101 VAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSGDTAN 265
+ Q+ D LR G + + + T + ERI+ E ++A+ KA GD +
Sbjct: 23 IPQLKDILRVFGLRLSGTKAELITRIKQLIERIAIENNTTSWEALKKAIDGDVTS 77
>SPAC30.02c |||RNA polymerase II elongator complex
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 281
Score = 24.6 bits (51), Expect = 4.8
Identities = 17/67 (25%), Positives = 31/67 (46%), Gaps = 4/67 (5%)
Frame = +3
Query: 63 SSSSILVEMERYMSLRSEMR*E-L*DKILQSQM*KNVLFI---LNLMKGYLLRCFCQFTR 230
+ S+ +E E Y ++E L +Q ++ K+ I LN +KG+ + +C+
Sbjct: 45 NDESLNIEKETYRESKNEKAARGLLYSAVQRELSKSTFVICDALNYIKGFRYQLYCESKS 104
Query: 231 PYRKHAV 251
Y H V
Sbjct: 105 MYTTHCV 111
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 24.2 bits (50), Expect = 6.3
Identities = 17/50 (34%), Positives = 25/50 (50%)
Frame = -3
Query: 361 LLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 212
L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1384 LVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 910
Score = 24.2 bits (50), Expect = 6.3
Identities = 15/47 (31%), Positives = 19/47 (40%)
Frame = +2
Query: 176 HLKPDERISFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFI 316
H K RI+ PI Q S D D E FD+D + F+
Sbjct: 342 HAKGKSRITLNTQGPITQQGSSNADADELEDEDEDDDEFDEDDDAFM 388
>SPAC27F1.05c |||aminotransferase class-III, unknown
specificty|Schizosaccharomyces pombe|chr 1|||Manual
Length = 484
Score = 23.8 bits (49), Expect = 8.3
Identities = 12/34 (35%), Positives = 13/34 (38%)
Frame = -3
Query: 292 KMAQTLNKVISSVATACFRYGLVNWQKHLKRYPF 191
K TLN A F G WQK+ PF
Sbjct: 182 KFLSTLNSFHGKTTGAVFLGGKEKWQKYQSPAPF 215
>SPAC3A11.05c |kms1||meiotic spindle pole body protein
Kms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 607
Score = 23.8 bits (49), Expect = 8.3
Identities = 21/83 (25%), Positives = 37/83 (44%), Gaps = 10/83 (12%)
Frame = +2
Query: 47 EFQEAFQLFDSRGDGKIHVAQ----IGD--ALRALGQNPTESDV-KKCTLHL---KPDER 196
+F F +D + +GK+H++ I D L P S+ K+CTL D
Sbjct: 6 DFDLIFDSYDFKHEGKVHLSNFLPIINDLQLLHPASAPPLLSEFQKQCTLEFVRQNADLS 65
Query: 197 ISFEVFLPIYQAISKARSGDTAN 265
I+ + F +Y+ +++ AN
Sbjct: 66 ITKDNFRDVYKKLTENEDDSFAN 88
>SPBC31E1.06 |bms1|SPBC800.01|GTP binding protein
Bms1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1121
Score = 23.8 bits (49), Expect = 8.3
Identities = 12/46 (26%), Positives = 22/46 (47%)
Frame = +2
Query: 20 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESD 157
AG+ E + + QEA Q G+ + + DA+ + + +E D
Sbjct: 364 AGFGERMVMQLQEAQQPLGVDGNSGLQLFSNSDAIDTVDRESSEID 409
>SPAC13G6.14 |aps1|SPAC24B11.03|diadenosine
5',5'''-p1,p6-hexaphosphate hydrolase Aps1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 23.8 bits (49), Expect = 8.3
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +1
Query: 19 GWIFRGSIGRISRSLPALRFSWRWKDTCRSDRRCVKSFRTKS 144
GW G +G I+RSL + + R DT ++ +K +KS
Sbjct: 90 GWEEGGLVGHITRSLGSFK-DKRPTDTIDRRKKYLKQLMSKS 130
>SPCC1322.03 |||TRP-like ion channel|Schizosaccharomyces pombe|chr
3|||Manual
Length = 862
Score = 23.8 bits (49), Expect = 8.3
Identities = 16/44 (36%), Positives = 22/44 (50%), Gaps = 1/44 (2%)
Frame = +2
Query: 83 GDGK-IHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEV 211
G+G IH A + ALG+ T + K C + + P SFEV
Sbjct: 80 GNGSHIHAASVYSETYALGKLVTSNTTKLCDVVMDPH---SFEV 120
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,566,844
Number of Sequences: 5004
Number of extensions: 29671
Number of successful extensions: 120
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 112046990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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