Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0075
(363 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g28780.1 68416.m03592 glycine-rich protein similar to H41 gen... 32 0.099
At3g57120.1 68416.m06359 protein kinase family protein contains ... 30 0.53
At3g28840.1 68416.m03598 expressed protein 30 0.53
At3g28830.1 68416.m03597 expressed protein 29 1.2
At3g16460.2 68416.m02097 jacalin lectin family protein contains ... 29 1.2
At3g16460.1 68416.m02098 jacalin lectin family protein contains ... 29 1.2
At2g04540.1 68415.m00460 3-oxoacyl-[acyl-carrier-protein] syntha... 29 1.2
At5g60940.2 68418.m07645 transducin family protein / WD-40 repea... 28 1.6
At5g60940.1 68418.m07644 transducin family protein / WD-40 repea... 28 1.6
At5g49410.1 68418.m06114 expressed protein similar to unknown pr... 26 6.5
At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /... 26 6.5
At3g03550.1 68416.m00357 zinc finger (C3HC4-type RING finger) fa... 26 6.5
At1g35220.1 68414.m04368 expressed protein 26 6.5
>At3g28780.1 68416.m03592 glycine-rich protein similar to H41 gene
for histone protein GB:X15142 GI:3204 [Physarum
polycephalum]
Length = 614
Score = 32.3 bits (70), Expect = 0.099
Identities = 24/71 (33%), Positives = 36/71 (50%), Gaps = 4/71 (5%)
Frame = -1
Query: 285 GVLSEDGGLSVTVGTAAE-AIASDSIATGQTAKTSTGATRDSEVTG---AGSGTATESLS 118
G +E GG S + G A+ + S+S + G + ST A + G AGSGT+TE+ S
Sbjct: 432 GGSAETGGESTSSGVASGGSTGSESASAGAASGGSTEANGGAAAGGSTEAGSGTSTETSS 491
Query: 117 ITAGRTSGAFV 85
+ G + V
Sbjct: 492 MGGGSAAAGGV 502
Score = 27.9 bits (59), Expect = 2.1
Identities = 24/74 (32%), Positives = 34/74 (45%), Gaps = 6/74 (8%)
Frame = -1
Query: 297 SVSVGVLSED--GGLSVTVGTAAEAIASDSIATGQTAKTST----GATRDSEVTGAGSGT 136
S + G +SE GG + GT+ A + A G + T T G+ S G SG+
Sbjct: 496 SAAAGGVSESSSGGSTAAGGTSESASGGSATAGGASGGTYTDSTGGSPTGSPSAGGPSGS 555
Query: 135 ATESLSITAGRTSG 94
A+ES S+ G G
Sbjct: 556 ASES-SMEGGTFGG 568
>At3g57120.1 68416.m06359 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 456
Score = 29.9 bits (64), Expect = 0.53
Identities = 16/40 (40%), Positives = 22/40 (55%)
Frame = -1
Query: 216 SIATGQTAKTSTGATRDSEVTGAGSGTATESLSITAGRTS 97
S +T T T+T T +S TGA S ++ + S A RTS
Sbjct: 50 SPSTSNTGTTTTTTTSNSNKTGASSSSSGAASSSVASRTS 89
>At3g28840.1 68416.m03598 expressed protein
Length = 391
Score = 29.9 bits (64), Expect = 0.53
Identities = 21/72 (29%), Positives = 30/72 (41%), Gaps = 1/72 (1%)
Frame = -1
Query: 291 SVGVLSEDGGLSVTVG-TAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSGTATESLSI 115
+ G + GG + G TAA + T T+ GAT T AG TA+++ +
Sbjct: 226 AAGGTTATGGTTAAGGSTAAGGTTASGAGTAGYGATAGGATASGAGTAAGGTTASDAGTA 285
Query: 114 TAGRTSGAFVVA 79
SGA A
Sbjct: 286 AGTTASGAGTAA 297
>At3g28830.1 68416.m03597 expressed protein
Length = 539
Score = 28.7 bits (61), Expect = 1.2
Identities = 21/75 (28%), Positives = 38/75 (50%), Gaps = 6/75 (8%)
Frame = -1
Query: 297 SVSVGVLSEDGGLSVTV--GTAAEAIASDSIAT-GQTAKTSTGATRDSEVTGAGSGTATE 127
S G +S G +S +++ + AS S+AT + + + AT+ E +G + T ++
Sbjct: 206 STKSGSVSSSGSVSTKSKESSSSGSSASGSVATKSKESSGGSAATKSKESSGGSAATKSK 265
Query: 126 SL---SITAGRTSGA 91
S T G+TSG+
Sbjct: 266 ESSGGSATTGKTSGS 280
>At3g16460.2 68416.m02097 jacalin lectin family protein contains
Pfam profile: PF01419 jacalin-like lectin domain;
similar to myrosinase binding protein [Brassica napus]
GI:1711296, GI:1655824, myrosinase-binding protein
homolog [Arabidopsis thaliana] GI:2997767; contains Pfam
profile PF01419 jacalin-like lectin family
Length = 647
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/64 (28%), Positives = 26/64 (40%)
Frame = -1
Query: 285 GVLSEDGGLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSGTATESLSITAG 106
G L G + T+ I + TG T + T TG G+GT T + + T G
Sbjct: 109 GFLGTSGNVLNTIDVHYSPIPTGGTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGG 168
Query: 105 RTSG 94
+G
Sbjct: 169 TGTG 172
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/59 (30%), Positives = 21/59 (35%), Gaps = 1/59 (1%)
Frame = -1
Query: 264 GLSVTVGTAAEAIASDSIATGQTAK-TSTGATRDSEVTGAGSGTATESLSITAGRTSGA 91
G GT TG T T TG T TG G+GT T G +G+
Sbjct: 192 GTGTGTGTGTGTGTGTGTGTGGTGTGTGTGGTGTGTGTGTGTGTGTGGTGTGTGTGTGS 250
Score = 26.6 bits (56), Expect = 4.9
Identities = 14/45 (31%), Positives = 17/45 (37%)
Frame = -1
Query: 264 GLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSGTAT 130
G GT + + G T TG T TG G+GT T
Sbjct: 159 GTGTGTGTGGTGTGTGTGTGGTGTGTGTGGTGTGTGTGTGTGTGT 203
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/58 (27%), Positives = 21/58 (36%)
Frame = -1
Query: 303 TVSVSVGVLSEDGGLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSGTAT 130
T+ V + G + GT TG T TG + TG G+GT T
Sbjct: 120 TIDVHYSPIPTGGTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGTGTGTGTGT 177
>At3g16460.1 68416.m02098 jacalin lectin family protein contains
Pfam profile: PF01419 jacalin-like lectin domain;
similar to myrosinase binding protein [Brassica napus]
GI:1711296, GI:1655824, myrosinase-binding protein
homolog [Arabidopsis thaliana] GI:2997767; contains Pfam
profile PF01419 jacalin-like lectin family
Length = 705
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/64 (28%), Positives = 26/64 (40%)
Frame = -1
Query: 285 GVLSEDGGLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSGTATESLSITAG 106
G L G + T+ I + TG T + T TG G+GT T + + T G
Sbjct: 109 GFLGTSGNVLNTIDVHYSPIPTGGTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGG 168
Query: 105 RTSG 94
+G
Sbjct: 169 TGTG 172
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/59 (30%), Positives = 21/59 (35%), Gaps = 1/59 (1%)
Frame = -1
Query: 264 GLSVTVGTAAEAIASDSIATGQTAK-TSTGATRDSEVTGAGSGTATESLSITAGRTSGA 91
G GT TG T T TG T TG G+GT T G +G+
Sbjct: 192 GTGTGTGTGTGTGTGTGTGTGGTGTGTGTGGTGTGTGTGTGTGTGTGGTGTGTGTGTGS 250
Score = 26.6 bits (56), Expect = 4.9
Identities = 14/45 (31%), Positives = 17/45 (37%)
Frame = -1
Query: 264 GLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSGTAT 130
G GT + + G T TG T TG G+GT T
Sbjct: 159 GTGTGTGTGGTGTGTGTGTGGTGTGTGTGGTGTGTGTGTGTGTGT 203
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/58 (27%), Positives = 21/58 (36%)
Frame = -1
Query: 303 TVSVSVGVLSEDGGLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSGTAT 130
T+ V + G + GT TG T TG + TG G+GT T
Sbjct: 120 TIDVHYSPIPTGGTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGTGTGTGTGT 177
>At2g04540.1 68415.m00460 3-oxoacyl-[acyl-carrier-protein] synthase
II, putative similar to Swiss-Prot:P56902
3-oxoacyl-[acyl-carrier-protein] synthase II (EC
2.3.1.41) (Beta- ketoacyl-ACP synthase II) (KAS II)
[Rhizobium meliloti]
Length = 461
Score = 28.7 bits (61), Expect = 1.2
Identities = 22/77 (28%), Positives = 33/77 (42%)
Frame = -1
Query: 300 VSVSVGVLSEDGGLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSGTATESL 121
V V+ G S LSV + + A+++ ++ Q A RD V G GSG
Sbjct: 230 VMVAGGTESSIDALSVAGFSRSRALSTKFNSSPQEASRPFDCDRDGFVIGEGSGVIVLE- 288
Query: 120 SITAGRTSGAFVVAEIC 70
+ GA + AE+C
Sbjct: 289 EYEHAKRRGAKIYAELC 305
>At5g60940.2 68418.m07645 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400);
similar to cleavage stimulation factor 50K chain Homo
sapiens, PIR:A45142
Length = 337
Score = 28.3 bits (60), Expect = 1.6
Identities = 17/59 (28%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Frame = -1
Query: 276 SEDGGLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSE-VTGAGSGTATESLSITAGR 103
S+DG + + G +A+ + S A G++ TS T+D V +G + + I +GR
Sbjct: 193 SKDGAIRLFDGVSAKCVRSIGNAHGKSEVTSAVFTKDQRFVLSSGKDSTVKLWEIGSGR 251
>At5g60940.1 68418.m07644 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400);
similar to cleavage stimulation factor 50K chain Homo
sapiens, PIR:A45142
Length = 429
Score = 28.3 bits (60), Expect = 1.6
Identities = 17/59 (28%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Frame = -1
Query: 276 SEDGGLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSE-VTGAGSGTATESLSITAGR 103
S+DG + + G +A+ + S A G++ TS T+D V +G + + I +GR
Sbjct: 285 SKDGAIRLFDGVSAKCVRSIGNAHGKSEVTSAVFTKDQRFVLSSGKDSTVKLWEIGSGR 343
>At5g49410.1 68418.m06114 expressed protein similar to unknown
protein (gb|AAF36742.1)
Length = 145
Score = 26.2 bits (55), Expect = 6.5
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -2
Query: 197 RPKPQQGPLEIQK*PEQAQVL 135
+PKP+ P+E+ PEQ Q +
Sbjct: 27 KPKPKMKPIELNTPPEQTQTI 47
>At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh1p (GI:2739044)
{Glycine max}; similar to polyphosphoinositide binding
protein Ssh2, Glycine max, gb:T05953; contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus
Length = 668
Score = 26.2 bits (55), Expect = 6.5
Identities = 18/39 (46%), Positives = 20/39 (51%)
Frame = +2
Query: 68 SQISATTNAPEVLPAVIDSDSVAVPEPAPVTSESLVAPV 184
SQ SATT P L + + S EP VTSE APV
Sbjct: 2 SQDSATTTPPPPLTSDVSMPSGEEDEPKHVTSEE-EAPV 39
>At3g03550.1 68416.m00357 zinc finger (C3HC4-type RING finger)
family protein contains zinc finger domain, C3HC4 type
(RING finger) 152633.
Length = 356
Score = 26.2 bits (55), Expect = 6.5
Identities = 18/68 (26%), Positives = 28/68 (41%), Gaps = 5/68 (7%)
Frame = -1
Query: 258 SVTVGTAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSG-----TATESLSITAGRTSG 94
S T A I+SD G + GAT ++ G G G + +S+++ R
Sbjct: 92 SSTSAAAINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMD 151
Query: 93 AFVVAEIC 70
FV + C
Sbjct: 152 GFVESSDC 159
>At1g35220.1 68414.m04368 expressed protein
Length = 1028
Score = 26.2 bits (55), Expect = 6.5
Identities = 19/75 (25%), Positives = 31/75 (41%)
Frame = -1
Query: 306 CTVSVSVGVLSEDGGLSVTVGTAAEAIASDSIATGQTAKTSTGATRDSEVTGAGSGTATE 127
C + +V ++ L +G+ AI SD + + +ST + D E G TE
Sbjct: 323 CRLGWAVKLIDPSSVLHDKIGSP-RAILSDDEDASRASISSTYRSADGEEAQHGDNLGTE 381
Query: 126 SLSITAGRTSGAFVV 82
S + AF+V
Sbjct: 382 SSGSRSSHVRVAFIV 396
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,188,223
Number of Sequences: 28952
Number of extensions: 83007
Number of successful extensions: 308
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 256
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 287
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 467982008
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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