BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0070
(577 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32F12.15 |tfb5||transcription factor TFIIH complex subunit T... 42 9e-05
SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces po... 26 4.5
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 25 6.0
SPBC1347.04 |tim54||TIM22 inner membrane protein import complex ... 25 6.0
>SPBC32F12.15 |tfb5||transcription factor TFIIH complex subunit Tfb5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 68
Score = 41.5 bits (93), Expect = 9e-05
Identities = 20/71 (28%), Positives = 42/71 (59%), Gaps = 1/71 (1%)
Frame = +2
Query: 74 MVNVMKGVL-VECDPAMKQFLLHLDETLALGRKFILQDLDETHLFISADIVETLQARVDD 250
M KG+L VECDP +KQ +L++DE +++++DE L ++ +E ++A ++
Sbjct: 1 MPRAQKGLLLVECDPTVKQLILNMDEQ---SPGIVIEEIDEERLLVNESRLEQVKAELER 57
Query: 251 LMDQLSIPVHD 283
+++ + V +
Sbjct: 58 RLEENTYQVEE 68
>SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 942
Score = 25.8 bits (54), Expect = 4.5
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = -2
Query: 573 SCANVFRNRIL*YSALLDFFFLVGFSLESANFGTFFDITKSACGSS 436
SC +V N Y+ LDF+ +GF + A+FGT C S
Sbjct: 10 SCYSVVANTEDTYNQTLDFYQKLGFK-KVASFGTSDSDNARVCNES 54
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.4 bits (53), Expect = 6.0
Identities = 17/66 (25%), Positives = 30/66 (45%)
Frame = -2
Query: 516 FFLVGFSLESANFGTFFDITKSACGSS*KLSCSRASTAFLARRFLATSSKTFLLSASLTL 337
F++VG S+ G+ F + S + AST+ + T++ T SA+ +
Sbjct: 111 FYIVGGEGISSTTGSTFQSMTTFTSSQTNSGHASASTSIPSTAITVTANSTIYSSATSSF 170
Query: 336 PDSREV 319
P S +V
Sbjct: 171 PYSTDV 176
>SPBC1347.04 |tim54||TIM22 inner membrane protein import complex
subunit Tim54|Schizosaccharomyces pombe|chr 2|||Manual
Length = 347
Score = 25.4 bits (53), Expect = 6.0
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +2
Query: 122 KQFLLHLDETLALGRKFILQDLDETHLFISADIVETLQARVD 247
KQF D + LQD DE F + DI ++ +A+ D
Sbjct: 303 KQFFTRSDLENRIWTAPFLQDSDEIRFFENIDIFDSTKAKQD 344
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,376,678
Number of Sequences: 5004
Number of extensions: 47274
Number of successful extensions: 136
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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