SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0061
         (392 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alp...    30   0.49 
At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alp...    30   0.49 
At3g19840.1 68416.m02513 FF domain-containing protein / WW domai...    28   2.6  
At3g63500.2 68416.m07153 expressed protein                             27   3.4  
At3g63500.1 68416.m07152 expressed protein                             27   3.4  
At4g21430.1 68417.m03097 transcription factor jumonji (jmjC) dom...    27   4.5  
At4g31210.1 68417.m04432 DNA topoisomerase family protein simila...    26   7.9  
At3g02500.1 68416.m00238 expressed protein                             26   7.9  

>At1g59900.1 68414.m06748 pyruvate dehydrogenase E1 component alpha
           subunit, mitochondrial (PDHE1-A) identical to SP|P52901
           Pyruvate dehydrogenase E1 component alpha subunit,
           mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
           {Arabidopsis thaliana}
          Length = 389

 Score = 30.3 bits (65), Expect = 0.49
 Identities = 14/46 (30%), Positives = 25/46 (54%)
 Frame = +2

Query: 254 PYKLHKLDQGPATSATLTSEDALKLYEQLTILRRIETG*GNLYKEK 391
           P+  H  D  P+ S   +S++ L  +  + ++RR+E    +LYK K
Sbjct: 39  PFTAHLCDP-PSRSVESSSQELLDFFRTMALMRRMEIAADSLYKAK 83


>At1g24180.1 68414.m03050 pyruvate dehydrogenase E1 component alpha
           subunit, mitochondrial, putative similar to SP|P52901
           Pyruvate dehydrogenase E1 component alpha subunit,
           mitochondrial precursor (EC 1.2.4.1) (PDHE1-A)
           {Arabidopsis thaliana}; contains Pfam profile PF00676:
           Dehydrogenase E1 component
          Length = 393

 Score = 30.3 bits (65), Expect = 0.49
 Identities = 15/46 (32%), Positives = 25/46 (54%)
 Frame = +2

Query: 254 PYKLHKLDQGPATSATLTSEDALKLYEQLTILRRIETG*GNLYKEK 391
           P+  H L + P+ S   +SE+ L  +  +  +RR+E    +LYK K
Sbjct: 43  PFTSH-LCESPSRSVETSSEEILAFFRDMARMRRMEIAADSLYKAK 87


>At3g19840.1 68416.m02513 FF domain-containing protein / WW
           domain-containing protein weak similarity to
           transcription factor CA150b [Mus musculus] GI:6329166;
           contains Pfam profiles PF01846: FF domain, PF00397: WW
           domain
          Length = 743

 Score = 27.9 bits (59), Expect = 2.6
 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 6/64 (9%)
 Frame = +2

Query: 140 IPSAAKFLAGNTITKVTAPVVATNAKYSTKKEAT------FEIKPYKLHKLDQGPATSAT 301
           +PSA     G+ +T ++AP ++   + +   + T       ++   KLH  D G   S+T
Sbjct: 255 VPSADLTEKGSDLTSLSAPAISNGGRDAASLKTTNFGSSALDLVKKKLH--DSGMPVSST 312

Query: 302 LTSE 313
           +TSE
Sbjct: 313 ITSE 316


>At3g63500.2 68416.m07153 expressed protein
          Length = 1162

 Score = 27.5 bits (58), Expect = 3.4
 Identities = 11/29 (37%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
 Frame = +3

Query: 231  KRRHSKSSPTNSINWIRAL-QHQPHSLLK 314
            K RH + SP+    W+R++    PH+ LK
Sbjct: 994  KPRHERGSPSQDTAWLRSVCSDNPHNQLK 1022


>At3g63500.1 68416.m07152 expressed protein
          Length = 887

 Score = 27.5 bits (58), Expect = 3.4
 Identities = 11/29 (37%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
 Frame = +3

Query: 231 KRRHSKSSPTNSINWIRAL-QHQPHSLLK 314
           K RH + SP+    W+R++    PH+ LK
Sbjct: 719 KPRHERGSPSQDTAWLRSVCSDNPHNQLK 747


>At4g21430.1 68417.m03097 transcription factor jumonji (jmjC)
           domain-containing protein contains Pfam domain, PF02373:
           jmjC domain
          Length = 728

 Score = 27.1 bits (57), Expect = 4.5
 Identities = 22/76 (28%), Positives = 34/76 (44%), Gaps = 5/76 (6%)
 Frame = +2

Query: 119 IDKMSKLIPSAAKFLAGNTITKVTAPVVATNA-KYSTKKEAT-FEIKPYKLHKLDQGPAT 292
           I K    + +  KFL+   +++    V   N    S K +A   E+K   +HK+ +    
Sbjct: 652 IRKNKSCVNAVLKFLSPEHVSESIKRVKELNQLPQSVKSKANKIEVKKMAIHKISEAVKE 711

Query: 293 SATLTSED---ALKLY 331
              LTS D   AL+LY
Sbjct: 712 IRELTSSDSTGALRLY 727


>At4g31210.1 68417.m04432 DNA topoisomerase family protein similar
           to DNA Topoisomerase I (SP:Q9X3X7) {Zymomonas mobilis}
          Length = 1280

 Score = 26.2 bits (55), Expect = 7.9
 Identities = 15/39 (38%), Positives = 22/39 (56%)
 Frame = +2

Query: 215 KYSTKKEATFEIKPYKLHKLDQGPATSATLTSEDALKLY 331
           K    +EA   I+P  + +L   P+T A+L   D+LKLY
Sbjct: 816 KVKNAQEAHEAIRPTDIRRL---PSTIASLLDADSLKLY 851


>At3g02500.1 68416.m00238 expressed protein 
          Length = 278

 Score = 26.2 bits (55), Expect = 7.9
 Identities = 11/41 (26%), Positives = 19/41 (46%)
 Frame = +3

Query: 210 TQNTARKKRRHSKSSPTNSINWIRALQHQPHSLLKMLLSCM 332
           T+    +K+   K   +   +WIR +Q QP     + L C+
Sbjct: 231 TKEIQEEKKEKKKRRGSGFSSWIRKMQRQPRKSKCIFLICL 271


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,023,092
Number of Sequences: 28952
Number of extensions: 141363
Number of successful extensions: 304
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 302
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 304
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 567552648
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -