Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0045
(646 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58070.1 68418.m07267 lipocalin, putative similar to temperat... 53 2e-07
At5g37190.1 68418.m04465 COP1-interacting protein 4 (CIP4) simil... 31 0.87
At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor, pu... 29 2.0
At4g00930.1 68417.m00126 COP1-interacting protein 4.1 (CIP4.1) i... 29 2.6
At5g36740.1 68418.m04402 PHD finger family protein 28 4.6
At5g36670.1 68418.m04388 PHD finger family protein 28 4.6
At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative... 28 4.6
At3g58260.1 68416.m06495 meprin and TRAF homology domain-contain... 28 4.6
At2g29070.2 68415.m03533 ubiquitin fusion degradation UFD1 famil... 28 4.6
At2g29070.1 68415.m03534 ubiquitin fusion degradation UFD1 famil... 28 4.6
At2g16750.1 68415.m01921 protein kinase family protein contains ... 28 4.6
At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative s... 28 6.1
At5g48240.1 68418.m05960 hypothetical protein 28 6.1
At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4... 28 6.1
At3g18370.1 68416.m02336 C2 domain-containing protein contains P... 28 6.1
>At5g58070.1 68418.m07267 lipocalin, putative similar to temperature
stress-induced lipocalin [Triticum aestivum] GI:18650668
Length = 186
Score = 52.8 bits (121), Expect = 2e-07
Identities = 31/82 (37%), Positives = 44/82 (53%), Gaps = 2/82 (2%)
Frame = +2
Query: 26 ELKPVNNFNLTAYQGIWYEISKFPNESE-KNGKCSSAEYKLEGD-VVKVKNVHIIDGVKK 199
E++ V N+ Y G WYEI+ FP+ + KNG + A Y L D + V N +G +
Sbjct: 6 EMEVVKGLNVERYMGRWYEIASFPSRFQPKNGVDTRATYTLNPDGTIHVLNETWSNGKRG 65
Query: 200 YIEGTAKLTDYANKAAKLTVTF 265
+IEG+A D + AKL V F
Sbjct: 66 FIEGSAYKADPKSDEAKLKVKF 87
>At5g37190.1 68418.m04465 COP1-interacting protein 4 (CIP4) similar
to COP1-interacting protein 4 (CIP4) [Arabidopsis
thaliana] GI:13160646; supporting cDNA
gi|13160645|dbj|AB036832.1|;
Length = 876
Score = 30.7 bits (66), Expect = 0.87
Identities = 21/75 (28%), Positives = 36/75 (48%)
Frame = +2
Query: 107 EKNGKCSSAEYKLEGDVVKVKNVHIIDGVKKYIEGTAKLTDYANKAAKLTVTFKFGEISR 286
EK+GK SS K + + V+ ++D +++ E L K++K T K ++
Sbjct: 515 EKSGKKSSKRSKKKDSLNIVEEAQVVDSLQQKKEAEENLEKSGKKSSKKTK--KKDSLNI 572
Query: 287 DGSVQVLATDYNNYA 331
QVL+ + NN A
Sbjct: 573 VEEAQVLSVEVNNVA 587
>At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor,
putative (AVDE1) similar to EST gb|N37612
Length = 462
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +2
Query: 38 VNNFNLTAYQGIWYEISKFPNESEKNGKCSSAEYKLEGDVVKVKNV 175
V NFN++ + G WY I+ N + C E+ EGD V N+
Sbjct: 204 VQNFNISDFNGKWY-ITSGLNPTFDAFDCQLHEFHTEGDNKLVGNI 248
>At4g00930.1 68417.m00126 COP1-interacting protein 4.1 (CIP4.1)
identical to cDNA CIP4.1 mRNA for COP1-interacting
protein 4.1, GI:13160649
Length = 976
Score = 29.1 bits (62), Expect = 2.6
Identities = 20/75 (26%), Positives = 36/75 (48%)
Frame = +2
Query: 107 EKNGKCSSAEYKLEGDVVKVKNVHIIDGVKKYIEGTAKLTDYANKAAKLTVTFKFGEISR 286
EK+GK SS K + + V+ ++D +++ E L K++K + K ++
Sbjct: 665 EKSGKKSSKRSKKKDSLNIVEEAQVVDSLQQKKEAEENLEKSGKKSSKRSK--KKDSLNI 722
Query: 287 DGSVQVLATDYNNYA 331
QVL+ + NN A
Sbjct: 723 VEEAQVLSVEVNNVA 737
>At5g36740.1 68418.m04402 PHD finger family protein
Length = 1179
Score = 28.3 bits (60), Expect = 4.6
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 122 CSSAEYKLEGDVVKVKNVHIIDGVKKYIEGTAKLTD 229
C Y EGD + N+ ++DG + E T KLTD
Sbjct: 1020 CLKLTYVEEGDNDRESNLKLLDGSVEEKEDTKKLTD 1055
>At5g36670.1 68418.m04388 PHD finger family protein
Length = 1193
Score = 28.3 bits (60), Expect = 4.6
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 122 CSSAEYKLEGDVVKVKNVHIIDGVKKYIEGTAKLTD 229
C Y EGD + N+ ++DG + E T KLTD
Sbjct: 1034 CLKLTYVEEGDNDRESNLKLLDGSVEEKEDTKKLTD 1069
>At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative
strong similarity to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 345
Score = 28.3 bits (60), Expect = 4.6
Identities = 14/35 (40%), Positives = 24/35 (68%), Gaps = 2/35 (5%)
Frame = +2
Query: 83 ISKFPNESEKNGKCSSAEYKLEG--DVVKVKNVHI 181
+S FP ES+ + K ++ E+KL G + V VKN+++
Sbjct: 14 VSGFPKESDFDFKTTTVEFKLPGGSNSVLVKNLYL 48
>At3g58260.1 68416.m06495 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 321
Score = 28.3 bits (60), Expect = 4.6
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -1
Query: 466 SLECSLMKLSTAVLASPSSFLFLERIQTTHLMRLFLVIVFAVV 338
SLE L K T VLA+ + FLF + L + +++ ++
Sbjct: 272 SLEALLKKEKTGVLAAKAPFLFFNNVNDDDLKWILRAVMYTLI 314
>At2g29070.2 68415.m03533 ubiquitin fusion degradation UFD1 family
protein similar to SP|P70362 Ubiquitin fusion
degradation protein 1 homolog (UB fusion protein 1) {Mus
musculus}; contains Pfam profile PF03152: Ubiquitin
fusion degradation protein UFD1
Length = 312
Score = 28.3 bits (60), Expect = 4.6
Identities = 19/61 (31%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
Frame = +2
Query: 146 EGDVVKVKNVHIIDGVK-KYIEGTAKLTDYANKAAKLTVTFKFGEISRDGSVQVLATDYN 322
EGDV++VKN+ ++ G K T D +N A L T + G + YN
Sbjct: 91 EGDVMQVKNISLVKGTYIKLQPHTQDFLDISNPKAILETTLRSYSCLTTG--DTIMVPYN 148
Query: 323 N 325
N
Sbjct: 149 N 149
>At2g29070.1 68415.m03534 ubiquitin fusion degradation UFD1 family
protein similar to SP|P70362 Ubiquitin fusion
degradation protein 1 homolog (UB fusion protein 1) {Mus
musculus}; contains Pfam profile PF03152: Ubiquitin
fusion degradation protein UFD1
Length = 280
Score = 28.3 bits (60), Expect = 4.6
Identities = 19/61 (31%), Positives = 27/61 (44%), Gaps = 1/61 (1%)
Frame = +2
Query: 146 EGDVVKVKNVHIIDGVK-KYIEGTAKLTDYANKAAKLTVTFKFGEISRDGSVQVLATDYN 322
EGDV++VKN+ ++ G K T D +N A L T + G + YN
Sbjct: 59 EGDVMQVKNISLVKGTYIKLQPHTQDFLDISNPKAILETTLRSYSCLTTG--DTIMVPYN 116
Query: 323 N 325
N
Sbjct: 117 N 117
>At2g16750.1 68415.m01921 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 617
Score = 28.3 bits (60), Expect = 4.6
Identities = 25/96 (26%), Positives = 42/96 (43%), Gaps = 1/96 (1%)
Frame = +3
Query: 264 LSLEKYHAMDQFKSWRLTIITTPSLTTANTMTRKSLIKCVVWILSRNKKL-EGDAKTAVD 440
LSL +DQ W L +T + TRK I V W++S ++ +T
Sbjct: 190 LSLPSVEVVDQTPGWPLLRTSTLATPMVQHQTRK--ISVVNWVMSLPERFPHHPNQTCQQ 247
Query: 441 NFINEHSKEIDSSKLVHTDFSEEACKFTSSSVITEH 548
+F ++ K+I K ++ FS + K +S E+
Sbjct: 248 SFCDKQLKDI--LKDINRWFSYDVLKTATSDFSLEN 281
>At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative
similar to SP|P04051 DNA-directed RNA polymerase III
largest subunit (EC 2.7.7.6) {Saccharomyces cerevisiae};
contains InterPro accession IPR000722: RNA polymerase,
alpha subunit
Length = 1328
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/46 (28%), Positives = 21/46 (45%)
Frame = +2
Query: 200 YIEGTAKLTDYANKAAKLTVTFKFGEISRDGSVQVLATDYNNYAIA 337
Y + ++ KA F G ++DG +L DYN++A A
Sbjct: 609 YFRNSELISGQLGKATLALDIFPLGNGNKDGLYSILLRDYNSHAAA 654
>At5g48240.1 68418.m05960 hypothetical protein
Length = 339
Score = 27.9 bits (59), Expect = 6.1
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = -3
Query: 434 SSFSVAFKLLVSREDPDDTLDETFSCHR 351
++F +AFK ++ + DDTL S H+
Sbjct: 129 NAFKIAFKAIMKKTKGDDTLGPVLSAHK 156
>At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4)
identical to CBL-interacting protein kinase 4
[Arabidopsis thaliana] gi|13249503|gb|AAG01367;
identical to cDNA calcineurin B-like (CBL) interacting
protein kinase 4 (CIPK4) GI:13249502
Length = 426
Score = 27.9 bits (59), Expect = 6.1
Identities = 32/129 (24%), Positives = 58/129 (44%), Gaps = 2/129 (1%)
Frame = -1
Query: 544 SVITLELVNLQA-SSEKSVCTSFEESISLECSLMKLSTAVLASPSSFLFLERIQTTHLMR 368
S+ T ELV ++ +K++ + E I E M+ L + + L + + T +
Sbjct: 40 SISTGELVAIKIIDKQKTIDSGMEPRIIREIEAMRR----LHNHPNVLKIHEVMATK-SK 94
Query: 367 LFLVIVFAVVSDGVVIIVSRQDLN*SIA*-YFSKLKSDC*LCGFIGVVGELGRPFYILLD 191
++LV+ +A + ++ LN S A YF +L S C G+ +P +LLD
Sbjct: 95 IYLVVEYAAGGELFTKLIRFGRLNESAARRYFQQLASALSFCHRDGIAHRDVKPQNLLLD 154
Query: 190 AVDDMHVLD 164
++ V D
Sbjct: 155 KQGNLKVSD 163
>At3g18370.1 68416.m02336 C2 domain-containing protein contains Pfam
profile: PF00168 C2 domain
Length = 815
Score = 27.9 bits (59), Expect = 6.1
Identities = 19/78 (24%), Positives = 37/78 (47%), Gaps = 1/78 (1%)
Frame = +3
Query: 285 AMDQFKSWRLTIITTPSLTTANTMTRKSLIKCVVWILSRNKKLEGDAKTAVDNFINE-HS 461
A D K WR T+ ++T + + +W+ NKKL + V+ + + S
Sbjct: 75 AEDLTKKWRQTVFNASTITPLEHCQWLNKLLSEIWLNYMNKKLSLRFSSMVEKRLRQRRS 134
Query: 462 KEIDSSKLVHTDFSEEAC 515
+ I++ +L+ +FS +C
Sbjct: 135 RLIENIQLL--EFSLGSC 150
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,873,348
Number of Sequences: 28952
Number of extensions: 251140
Number of successful extensions: 819
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 805
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 818
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1334473344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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