BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0043
(712 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1039.04 |||nicotinic acid plasma membrane transporter |Schiz... 28 1.5
SPAC30D11.10 |rad22||DNA repair protein Rad22|Schizosaccharomyce... 28 1.5
SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 27 3.5
SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 26 6.1
SPAC1002.11 |gaa1||GPI-anchor transamidase complex subunit Gaa1 ... 25 8.1
>SPAC1039.04 |||nicotinic acid plasma membrane transporter
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 27.9 bits (59), Expect = 1.5
Identities = 20/54 (37%), Positives = 26/54 (48%)
Frame = +1
Query: 10 CIMKTFIVFVVCVVLAQALTDEQKENLKKHRADCLSETKADEQLVNKLKTGDFK 171
C + F+V V V A LTDE+K L K R + S + E+L K FK
Sbjct: 233 CAIVIFLVLPVSVETANFLTDEEK-TLAKMRIENDSSSAISEKLSFKQSLTVFK 285
>SPAC30D11.10 |rad22||DNA repair protein Rad22|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 469
Score = 27.9 bits (59), Expect = 1.5
Identities = 21/70 (30%), Positives = 33/70 (47%), Gaps = 9/70 (12%)
Frame = +1
Query: 163 DFKTENEPLKKYALCMLIKSQLMTKDGKFKKDVALAKVPN------AEDKLKVEKLIDA- 321
DF EN+ + +L + + ++ KDG + +D+ + N A +K K E DA
Sbjct: 84 DFMDENKENGRISLGLSVIVRVTIKDGAYHEDIGYGSIDNCRGKASAFEKCKKEGTTDAL 143
Query: 322 --CLANKGNS 345
L N GNS
Sbjct: 144 KRALRNFGNS 153
>SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 629
Score = 26.6 bits (56), Expect = 3.5
Identities = 16/60 (26%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +1
Query: 13 IMKTFIVFVVCVVLAQALTDEQKENLKKHRADCLSETKADEQLVNKLKTGD-FKTENEPL 189
I+ TF++FVV ++L + L ++ K+ + + + E KL GD F+ P+
Sbjct: 274 IIDTFLIFVVSIILYRTL----NRDINKYNSAFVDQEDVQEDFGWKLVHGDVFRPPRRPM 329
>SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 848
Score = 25.8 bits (54), Expect = 6.1
Identities = 14/51 (27%), Positives = 24/51 (47%)
Frame = +1
Query: 49 VLAQALTDEQKENLKKHRADCLSETKADEQLVNKLKTGDFKTENEPLKKYA 201
V + +TD N+ + L E + + ++K KT DFK + + YA
Sbjct: 621 VASDKITDSSPGNMSEASESELEEVFKNPKELSKKKTTDFKDKEYYMSHYA 671
>SPAC1002.11 |gaa1||GPI-anchor transamidase complex subunit Gaa1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 581
Score = 25.4 bits (53), Expect = 8.1
Identities = 13/47 (27%), Positives = 24/47 (51%)
Frame = +2
Query: 542 FNLYLFDKFCLVVVTYSIENQNLIFFCVRHSFVYLV*WFLVISFISY 682
F+ +LF FC + Y + + I + F+YL+ F+ I F ++
Sbjct: 405 FSFWLFSIFCTIAAYYLVSSSTKITVFI---FLYLMLTFIGIIFSTF 448
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,894,768
Number of Sequences: 5004
Number of extensions: 60654
Number of successful extensions: 190
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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