Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0035
(652 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 335 4e-93
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 335 4e-93
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 335 4e-93
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 104 1e-23
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 60 2e-10
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 59 6e-10
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 2.3
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.1
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 5.4
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 7.2
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 7.2
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 25 9.5
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 9.5
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 25 9.5
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 335 bits (823), Expect = 4e-93
Identities = 153/194 (78%), Positives = 172/194 (88%)
Frame = +1
Query: 4 LPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDN 183
LPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDN
Sbjct: 246 LPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDN 305
Query: 184 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 363
VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAH
Sbjct: 306 VGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAH 365
Query: 364 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 543
IACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVR
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVR 425
Query: 544 DMRQTVAVGVIKAV 585
DMRQTVAVGVIKAV
Sbjct: 426 DMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 335 bits (823), Expect = 4e-93
Identities = 153/194 (78%), Positives = 172/194 (88%)
Frame = +1
Query: 4 LPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDN 183
LPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDN
Sbjct: 246 LPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDN 305
Query: 184 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 363
VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAH
Sbjct: 306 VGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAH 365
Query: 364 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 543
IACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVR
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVR 425
Query: 544 DMRQTVAVGVIKAV 585
DMRQTVAVGVIKAV
Sbjct: 426 DMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 335 bits (823), Expect = 4e-93
Identities = 153/194 (78%), Positives = 172/194 (88%)
Frame = +1
Query: 4 LPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDN 183
LPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDN
Sbjct: 246 LPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDN 305
Query: 184 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 363
VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAH
Sbjct: 306 VGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAH 365
Query: 364 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 543
IACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVR
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVR 425
Query: 544 DMRQTVAVGVIKAV 585
DMRQTVAVGVIKAV
Sbjct: 426 DMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 104 bits (249), Expect = 1e-23
Identities = 62/197 (31%), Positives = 105/197 (53%), Gaps = 2/197 (1%)
Frame = +1
Query: 4 LPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGD 180
+P+ YK +GT+ G++E G +K + V+ P N T EV ++ + E + ++ GD
Sbjct: 471 MPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGD 528
Query: 181 NVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTA 360
V V+ +++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA
Sbjct: 529 QVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTA 586
Query: 361 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 540
FA++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +
Sbjct: 587 VEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTL 645
Query: 541 RDMRQTVAVG-VIKAVN 588
RD TVAVG V+K ++
Sbjct: 646 RDQGTTVAVGKVVKILD 662
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 60.5 bits (140), Expect = 2e-10
Identities = 49/193 (25%), Positives = 86/193 (44%), Gaps = 2/193 (1%)
Frame = +1
Query: 4 LPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPANITTEVKSVEMHHEALQEAVPG 177
+ ++DV+ I G GTV GRVE G LK G +V +++ T V +EM + L AV G
Sbjct: 255 MAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAG 314
Query: 178 DNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHT 357
DN G ++++ ++L+RG + P F A +L + T +D +
Sbjct: 315 DNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYR 370
Query: 358 AHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFA 537
+ + +++ ++ T + K + GD + P+ +E Q RF
Sbjct: 371 PQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFT 421
Query: 538 VRDMRQTVAVGVI 576
VR+ TV ++
Sbjct: 422 VREGGSTVGTALV 434
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 58.8 bits (136), Expect = 6e-10
Identities = 55/193 (28%), Positives = 87/193 (45%), Gaps = 1/193 (0%)
Frame = +1
Query: 4 LPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE-AVPGD 180
L + DVY+ TV GRVE G ++ ++ + VK+V + + AV GD
Sbjct: 407 LSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGD 465
Query: 181 NVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTA 360
V + ++ V +LR G + + +N P + F A++ + G I +G T VL
Sbjct: 466 TVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL----- 519
Query: 361 HIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAV 540
H+ + K+ K + + S K I L PLC+ +E P LGRF +
Sbjct: 520 HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFIL 576
Query: 541 RDMRQTVAVGVIK 579
R TVA G++K
Sbjct: 577 RRSGDTVAAGIVK 589
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 2.3
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -3
Query: 128 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 12
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.1
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 222 QFLDGHVLYVETYIVSRYSFLESFV 148
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 5.4
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = -3
Query: 629 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 456
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 455 SPDLMDFGLTSVDLPVRRSTFSLIS 381
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 7.2
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3
Query: 77 NTPVSTLPTGTVPIPPIL 24
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3
Query: 464 MAASPDLMDFGLTSVDLPVRRSTFS 390
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/38 (34%), Positives = 16/38 (42%)
Frame = -3
Query: 164 SWRASWCISTDLTSVVMLAGAKTTMVPGFNTPVSTLPT 51
SW SW +ST L +V + G T ST T
Sbjct: 97 SWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRRT 134
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = -3
Query: 644 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 534
P + ++ T+PP S+ T + PT ++ + TA
Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -3
Query: 617 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 495
+ L P +S + TP+AT S+ + P GG D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.135 0.394
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,800,093
Number of Sequences: 5004
Number of extensions: 59691
Number of successful extensions: 182
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
- SilkBase 1999-2023 -