BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0034 (682 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 9e-05 At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 43 2e-04 At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 41 7e-04 At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17... 39 0.003 At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 37 0.011 At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 37 0.014 At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 34 0.076 At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 2.8 At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 3.8 At3g49540.1 68416.m05414 expressed protein 28 5.0 At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 8.7 At1g34180.1 68414.m04239 no apical meristem (NAM) family protein... 27 8.7 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 44.0 bits (99), Expect = 9e-05 Identities = 32/93 (34%), Positives = 50/93 (53%), Gaps = 8/93 (8%) Frame = +2 Query: 236 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 391 D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125 Query: 392 SVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 490 +++ + +GVLSV P KVP + I I+ Sbjct: 126 EIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156 >At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (HSP22.0-ER) identical to endomembrane-localized small heat shock protein GI:511795 from [Arabidopsis thaliana] Length = 195 Score = 43.2 bits (97), Expect = 2e-04 Identities = 31/104 (29%), Positives = 56/104 (53%), Gaps = 8/104 (7%) Frame = +2 Query: 203 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 358 K + ++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135 Query: 359 RYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 490 ++ LP+ ESV+++L +GVL++ + P V+G R + IA Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTINLTKLSPEKVKGPRVVNIA 178 >At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HSP17.4-CI) identical to 17.4 kDa class I heat shock protein SP:P19036 from [Arabidopsis thaliana] Length = 156 Score = 41.1 bits (92), Expect = 7e-04 Identities = 28/78 (35%), Positives = 42/78 (53%), Gaps = 8/78 (10%) Frame = +2 Query: 236 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 391 DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124 Query: 392 SVESRLSSDGVLSVIAPR 445 V++ + +GVLSV P+ Sbjct: 125 EVKASM-ENGVLSVTVPK 141 >At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17.6A (HSP17.7-CII) identical to heat shock protein 17.6A GI:3256075 from [Arabidopsis thaliana] Length = 156 Score = 39.1 bits (87), Expect = 0.003 Identities = 34/126 (26%), Positives = 58/126 (46%), Gaps = 9/126 (7%) Frame = +2 Query: 137 SREYYRPWRHLAAAARDLGSSIKSDKDKFQVNLDVQHFAPEEISVKTA-DGYIVVEGKHE 313 SR Y R + +AA D + D + +D+ +EI V+ + +VV GK + Sbjct: 33 SRAYMRDAKAMAATPAD----VIEHPDAYVFAVDMPGIKGDEIQVQIENENVLVVSGKRQ 88 Query: 314 -EKKDQHGY----ISRQ---FTRRYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEG 469 + K+ G + R+ F R++ LP+ E + S +DGVL V P+ PP + Sbjct: 89 RDNKENEGVKFVRMERRMGKFMRKFQLPDNADLEKI-SAACNDGVLKVTIPKLPPPEPKK 147 Query: 470 ERKIPI 487 + I + Sbjct: 148 PKTIQV 153 >At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HSP18.1-CI) identical to 18.2 kDa class I heat shock protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana]; contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 161 Score = 37.1 bits (82), Expect = 0.011 Identities = 24/78 (30%), Positives = 43/78 (55%), Gaps = 8/78 (10%) Frame = +2 Query: 236 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 391 D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127 Query: 392 SVESRLSSDGVLSVIAPR 445 V++ + +GVL+V+ P+ Sbjct: 128 EVKATM-ENGVLTVVVPK 144 >At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha crystallin family; identified in Scharf, K-D., et al, Cell Stress & Chaperones (2001) 6: 225-237. Length = 153 Score = 36.7 bits (81), Expect = 0.014 Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 8/78 (10%) Frame = +2 Query: 236 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 391 D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE + Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121 Query: 392 SVESRLSSDGVLSVIAPR 445 V++ + +GVL+V P+ Sbjct: 122 QVKAAM-ENGVLTVTVPK 138 >At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HSP17.8-CI) similar to 17.5 kDa class I heat shock protein SP:P04793 from [Glycine max]; contains Pfam PF00011: Hsp20/alpha crystallin family Length = 157 Score = 34.3 bits (75), Expect = 0.076 Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 8/78 (10%) Frame = +2 Query: 236 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 391 D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE + Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123 Query: 392 SVESRLSSDGVLSVIAPR 445 V++ + +GVL+V P+ Sbjct: 124 QVKASM-ENGVLTVTVPK 140 >At1g78910.1 68414.m09199 pseudouridine synthase family protein low similarity to SP|P23851 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli}; contains Pfam profile PF00849: RNA pseudouridylate synthase Length = 478 Score = 29.1 bits (62), Expect = 2.8 Identities = 23/59 (38%), Positives = 26/59 (44%), Gaps = 2/59 (3%) Frame = -3 Query: 401 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPG 231 T I+ YS A I N A L P+ PR S PPF LPG+NAG G Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADG 72 >At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein contains Pfam profile: PF04615 Utp14 protein Length = 822 Score = 28.7 bits (61), Expect = 3.8 Identities = 17/54 (31%), Positives = 23/54 (42%) Frame = +2 Query: 257 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 418 +EI D Y EG EE+ ++ R + L E C E+VES D Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101 >At3g49540.1 68416.m05414 expressed protein Length = 166 Score = 28.3 bits (60), Expect = 5.0 Identities = 17/49 (34%), Positives = 28/49 (57%) Frame = +2 Query: 374 EGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKIPIAQTGPVRKEVK 520 E E+ E+++ + ++V AP KV AVE E+K A+ PV+ E + Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKK---AEAEPVKAEAE 142 >At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing protein low similarity to fertility restorer [Petunia x hybrida] GI:22128587, post-transcriptional control of chloroplast gene expression CRP1 [Zea mays] GI:3289002; contains Pfam profile PF01535: PPR repeat Length = 991 Score = 27.5 bits (58), Expect = 8.7 Identities = 15/48 (31%), Positives = 20/48 (41%) Frame = -3 Query: 422 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 279 HHL + ++ P S P S + H SS PRCS+P Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73 >At1g34180.1 68414.m04239 no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) domain; similar to NAM-like protein GI:8809651 from (Arabidopsis thaliana) Length = 564 Score = 27.5 bits (58), Expect = 8.7 Identities = 16/50 (32%), Positives = 24/50 (48%) Frame = +2 Query: 296 VEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDGVLSVIAPR 445 +E E D G RQF +P+G +AE ++S L ++ I PR Sbjct: 229 IEKLLNEIPDAPGVNQRQFDEFVGVPQGNSAEVIQSTLLNNSSGEYIDPR 278 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,012,760 Number of Sequences: 28952 Number of extensions: 339929 Number of successful extensions: 948 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 912 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 948 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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