Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0027
(309 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44100.1 68415.m05484 Rab GDP dissociation inhibitor (GDI1) i... 122 4e-29
At3g59920.1 68416.m06687 Rab GDP dissociation inhibitor (GDI2) i... 121 9e-29
At5g09550.1 68418.m01106 Rab GDP dissociation inhibitor, putativ... 60 2e-10
At3g06540.1 68416.m00758 GDP dissociation inhibitor family prote... 33 0.052
At3g63460.2 68416.m07146 WD-40 repeat family protein hypothetica... 29 0.64
At3g63460.1 68416.m07145 WD-40 repeat family protein hypothetica... 29 0.64
At5g04340.1 68418.m00426 zinc finger (C2H2 type) family protein ... 28 1.5
At3g14950.1 68416.m01891 tetratricopeptide repeat (TPR)-containi... 27 2.6
At3g06660.1 68416.m00784 PAPA-1-like family protein / zinc finge... 26 5.9
At1g50120.1 68414.m05621 expressed protein 26 5.9
At3g19100.1 68416.m02427 calcium-dependent protein kinase, putat... 25 7.8
At2g27420.1 68415.m03314 cysteine proteinase, putative contains ... 25 7.8
>At2g44100.1 68415.m05484 Rab GDP dissociation inhibitor (GDI1)
identical to GDP dissociation inhibitor [Arabidopsis
thaliana] GI:1655424
Length = 445
Score = 122 bits (295), Expect = 4e-29
Identities = 59/103 (57%), Positives = 77/103 (74%), Gaps = 1/103 (0%)
Frame = +2
Query: 2 ILSGMLSVSGKKVLHIDRNKYYGGESASITPLEELFAKFNAPAPDETY-GRGRDWNVDLI 178
ILSG+LSV G KVLH+DRN YYGGES S+ L +L+ KF + G RD+NVD++
Sbjct: 18 ILSGLLSVDGLKVLHMDRNDYYGGESTSLN-LNQLWKKFRGEEKAPAHLGSSRDYNVDMM 76
Query: 179 PKFLMANGLLVKLLIHTGVTRXLEFKSIEGSYVYKGGKICKVP 307
PKF+MANG LV++LIHT VT+ L FK+++GSYV+ GK+ KVP
Sbjct: 77 PKFMMANGKLVRVLIHTDVTKYLSFKAVDGSYVFVQGKVQKVP 119
>At3g59920.1 68416.m06687 Rab GDP dissociation inhibitor (GDI2)
identical to Rab GDP dissociation inhibitor AtGDI2
[Arabidopsis thaliana] GI:2446981
Length = 444
Score = 121 bits (292), Expect = 9e-29
Identities = 61/104 (58%), Positives = 77/104 (74%), Gaps = 2/104 (1%)
Frame = +2
Query: 2 ILSGMLSVSGKKVLHIDRNKYYGGESASITPLEELFAKFNAP--APDETYGRGRDWNVDL 175
ILSG+LSV G KVLH+DRN YYGGES S+ L +L+ KF AP E G RD+NVD+
Sbjct: 18 ILSGLLSVDGVKVLHMDRNDYYGGESTSLN-LNQLWKKFRGEEKAP-EHLGASRDYNVDM 75
Query: 176 IPKFLMANGLLVKLLIHTGVTRXLEFKSIEGSYVYKGGKICKVP 307
+PKF+M NG LV+ LIHT VT+ L FK+++GSYV+ GK+ KVP
Sbjct: 76 MPKFMMGNGKLVRTLIHTDVTKYLSFKAVDGSYVFVKGKVQKVP 119
>At5g09550.1 68418.m01106 Rab GDP dissociation inhibitor, putative
strong similarity to GDP dissociation inhibitor protein
OsGDI1 [Oryza sativa] GI:2384758; contains Pfam profile
PF00996: GDP dissociation inhibitor
Length = 365
Score = 60.5 bits (140), Expect = 2e-10
Identities = 27/39 (69%), Positives = 33/39 (84%)
Frame = +2
Query: 191 MANGLLVKLLIHTGVTRXLEFKSIEGSYVYKGGKICKVP 307
MANGLLV+ LIHT VT+ L FK+++GS+VYK GKI KVP
Sbjct: 1 MANGLLVQTLIHTDVTKYLNFKAVDGSFVYKKGKIYKVP 39
>At3g06540.1 68416.m00758 GDP dissociation inhibitor family protein
/ Rab GTPase activator family protein similar to
SP|P26374 Rab proteins geranylgeranyltransferase
component A 2 (Rab escort protein 2) {Homo sapiens};
contains Pfam profile PF00996: GDP dissociation
inhibitor
Length = 563
Score = 32.7 bits (71), Expect = 0.052
Identities = 17/45 (37%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +2
Query: 2 ILSGMLSVSGKKVLHIDRNKYYGGESASIT-PLEELFAKFNAPAP 133
+L+ S SG VLH+D N +YG AS++ P F N+ +P
Sbjct: 28 VLAAAASSSGSSVLHLDPNPFYGSHFASLSLPDLTSFLHSNSVSP 72
Score = 32.3 bits (70), Expect = 0.068
Identities = 18/53 (33%), Positives = 30/53 (56%), Gaps = 2/53 (3%)
Frame = +2
Query: 155 RDWNVDLI-PKFLMANGLLVKLLIHTGVTRXLEFKSIEGSYV-YKGGKICKVP 307
R +NVDL P+ + + L++ +G +EFKSI+ S+V G++ VP
Sbjct: 118 RRFNVDLAGPRVVFCADESINLMLKSGANNYVEFKSIDASFVGDSSGELRNVP 170
>At3g63460.2 68416.m07146 WD-40 repeat family protein hypothetical
protein contains similarity to ec31p [Oryza sativa]
gi|13928450|dbj|BAB47154; contains Pfam profile PF00400:
WD domain, G-beta repeat
Length = 1102
Score = 29.1 bits (62), Expect = 0.64
Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 4/50 (8%)
Frame = +2
Query: 161 WN-VDLIPKFLMANGLLVKLLIHTGVTRXLEFKSIEGSYVYKG---GKIC 298
WN + LI N L+ L +H G R LEF +I + + G G+IC
Sbjct: 99 WNPLSLIGSQPSENALVGHLSVHKGPVRGLEFNAISSNLLASGADDGEIC 148
>At3g63460.1 68416.m07145 WD-40 repeat family protein hypothetical
protein contains similarity to ec31p [Oryza sativa]
gi|13928450|dbj|BAB47154; contains Pfam profile PF00400:
WD domain, G-beta repeat
Length = 1104
Score = 29.1 bits (62), Expect = 0.64
Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 4/50 (8%)
Frame = +2
Query: 161 WN-VDLIPKFLMANGLLVKLLIHTGVTRXLEFKSIEGSYVYKG---GKIC 298
WN + LI N L+ L +H G R LEF +I + + G G+IC
Sbjct: 99 WNPLSLIGSQPSENALVGHLSVHKGPVRGLEFNAISSNLLASGADDGEIC 148
>At5g04340.1 68418.m00426 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 238
Score = 27.9 bits (59), Expect = 1.5
Identities = 19/61 (31%), Positives = 32/61 (52%)
Frame = +2
Query: 20 SVSGKKVLHIDRNKYYGGESASITPLEELFAKFNAPAPDETYGRGRDWNVDLIPKFLMAN 199
++ G K H + K GG S+S++ E++ + + + RG D N+ IP+F M N
Sbjct: 162 ALGGHKRCHYE-GKNGGGVSSSVSNSEDVGSTSHVSSGH----RGFDLNIPPIPEFSMVN 216
Query: 200 G 202
G
Sbjct: 217 G 217
>At3g14950.1 68416.m01891 tetratricopeptide repeat (TPR)-containing
protein low similarity to SP|Q99615 DnaJ homolog
subfamily C member 7 (Tetratricopeptide repeat protein
2) {Homo sapiens}; contains Pfam profile PF00515: TPR
Domain
Length = 721
Score = 27.1 bits (57), Expect = 2.6
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = +2
Query: 56 NKYYGGESASITPLEELFAKFNAPAPD 136
N +GGE I+ LEEL A PA D
Sbjct: 621 NMEFGGEVEEISSLEELKAALTRPASD 647
>At3g06660.1 68416.m00784 PAPA-1-like family protein / zinc finger
(HIT type) family protein contains Pfam domains,
PF04795: PAPA-1-like conserved region and PF04438: HIT
zinc finger
Length = 427
Score = 25.8 bits (54), Expect = 5.9
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +2
Query: 197 NGLLVKLLIHTGVTRXLEFKSIEGSY 274
N L VKL + GVTR L+ S G+Y
Sbjct: 71 NKLKVKLKLGAGVTRTLQTNSEAGTY 96
>At1g50120.1 68414.m05621 expressed protein
Length = 532
Score = 25.8 bits (54), Expect = 5.9
Identities = 16/56 (28%), Positives = 26/56 (46%)
Frame = +2
Query: 26 SGKKVLHIDRNKYYGGESASITPLEELFAKFNAPAPDETYGRGRDWNVDLIPKFLM 193
S V+ + K G AS++P A P P E + RGR +N+ + + L+
Sbjct: 316 SSNSVVPSQQPKQTNGAGASMSP----GAGAREPVPSEGFTRGRSYNIRMDDQVLL 367
>At3g19100.1 68416.m02427 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium/calmodulin-dependent
protein kinase CaMK3 [Nicotiana tabacum]
gi|16904226|gb|AAL30820; contains protein kinase domain,
Pfam:PF00069; contains serine/threonine protein kinase
domain, INTERPRO:IPR002290
Length = 599
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/24 (50%), Positives = 16/24 (66%), Gaps = 2/24 (8%)
Frame = +2
Query: 68 GGESASIT--PLEELFAKFNAPAP 133
GGES S+T PL +L F+ P+P
Sbjct: 71 GGESKSVTSTPLRQLARAFHPPSP 94
>At2g27420.1 68415.m03314 cysteine proteinase, putative contains
similarity to cysteine protease SPCP1 GI:13491750 from
[Ipomoea batatas]
Length = 348
Score = 25.4 bits (53), Expect = 7.8
Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 4/55 (7%)
Frame = +2
Query: 74 ESASITPLEELFAKFNAPAPDETYGRGR----DWNVDLIPKFLMANGLLVKLLIH 226
E+++I E+ A+FN DET R R N++ + F M N + K+ I+
Sbjct: 28 EASAIEKHEQWMARFNRVYSDETEKRNRFNIFKKNLEFVQNFNMNNKITYKVDIN 82
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,775,590
Number of Sequences: 28952
Number of extensions: 125011
Number of successful extensions: 369
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 365
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 367
length of database: 12,070,560
effective HSP length: 70
effective length of database: 10,043,920
effective search space used: 321405440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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