BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brS-0020 (596 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha ... 29 0.15 AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha ... 25 2.5 DQ303468-1|ABC18327.1| 1115|Anopheles gambiae putative methopren... 24 3.2 M93690-2|AAA29363.1| 1212|Anopheles gambiae unknown protein. 24 4.3 AJ439398-8|CAD28131.1| 1283|Anopheles gambiae putative different... 24 4.3 M93689-1|AAA29368.1| 442|Anopheles gambiae protein ( Anopheles ... 23 9.9 AJ439353-10|CAD27932.1| 3325|Anopheles gambiae F25C8.3 protein p... 23 9.9 >AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha 1 chain protein. Length = 1024 Score = 28.7 bits (61), Expect = 0.15 Identities = 21/67 (31%), Positives = 25/67 (37%), Gaps = 5/67 (7%) Frame = +2 Query: 227 GSKRAPGGTAALGNPPGTPRPGFS-----MSRRGANGLNIIPPPKGENGSRSEWPTLRPG 391 G K PG A+G P PG S G GL +P P G NG + P Sbjct: 476 GDKGEPGFPGAIGRPGKVGVPGLSGEAGAKGEMGIQGLPGLPGPAGLNGLPGMKGDMGPL 535 Query: 392 GTAHRSC 412 G +C Sbjct: 536 GEKGDAC 542 Score = 27.1 bits (57), Expect = 0.46 Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 2/48 (4%) Frame = +2 Query: 227 GSKRAPGGTAALGN--PPGTPRPGFSMSRRGANGLNIIPPPKGENGSR 364 G + PG G+ PPG P R +GLN + P+G G R Sbjct: 618 GQRGLPGPQGEKGDQGPPGFIGPKGDKGERDRDGLNGLNGPQGMKGDR 665 Score = 26.6 bits (56), Expect = 0.61 Identities = 17/55 (30%), Positives = 21/55 (38%) Frame = +2 Query: 227 GSKRAPGGTAALGNPPGTPRPGFSMSRRGANGLNIIPPPKGENGSRSEWPTLRPG 391 G + PG G P G G +GLN P KG+ G + P R G Sbjct: 663 GDRGMPGLEGVAGLPGMVGEKG-DRGLPGMSGLNGAPGEKGQKGETPQLPPQRKG 716 Score = 26.2 bits (55), Expect = 0.80 Identities = 20/63 (31%), Positives = 24/63 (38%), Gaps = 2/63 (3%) Frame = +2 Query: 224 NGSKRAPGGTAALGNPPGTPRPGFSMSRRGANGLNII--PPPKGENGSRSEWPTLRPGGT 397 N P G PG P S +G GL+++ P PKG G R P GG Sbjct: 67 NSGPVGPPGAPGRDGMPGAPGLPGSKGVKGDPGLSMVGPPGPKGNPGLRG--PKGERGGM 124 Query: 398 AHR 406 R Sbjct: 125 GDR 127 Score = 24.6 bits (51), Expect = 2.5 Identities = 11/24 (45%), Positives = 13/24 (54%) Frame = -3 Query: 312 PRRDIENPGLGVPGGLPRAAVPPG 241 P+ D PGL P G+P A PG Sbjct: 726 PKGDKGLPGLAGPAGIPGAPGAPG 749 >AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha 1 chain precursor protein. Length = 801 Score = 24.6 bits (51), Expect = 2.5 Identities = 16/48 (33%), Positives = 19/48 (39%), Gaps = 2/48 (4%) Frame = +2 Query: 227 GSKRAPG--GTAALGNPPGTPRPGFSMSRRGANGLNIIPPPKGENGSR 364 G K PG G L P G P R G G+ P +GE G + Sbjct: 226 GQKGEPGNDGLEGLPGPQGEVGPRGFPGRPGEKGVPGTPGVRGERGDK 273 Score = 24.6 bits (51), Expect = 2.5 Identities = 14/44 (31%), Positives = 17/44 (38%) Frame = +2 Query: 227 GSKRAPGGTAALGNPPGTPRPGFSMSRRGANGLNIIPPPKGENG 358 G APGG PG P +G G++ KGE G Sbjct: 398 GPAGAPGGGEGRPGAPGPKGPRGYEGPQGPKGMDGFDGEKGERG 441 Score = 24.2 bits (50), Expect = 3.2 Identities = 18/52 (34%), Positives = 21/52 (40%), Gaps = 2/52 (3%) Frame = +2 Query: 227 GSKRAPGGTAALGNP--PGTPRPGFSMSRRGANGLNIIPPPKGENGSRSEWP 376 G+ PG G P GTP +G GL P P GE G +E P Sbjct: 604 GASGVPGERGYPGMPGEDGTPGLRGEPGPKGEPGLLGPPGPSGEPGRDAEIP 655 Score = 23.8 bits (49), Expect = 4.3 Identities = 18/53 (33%), Positives = 26/53 (49%), Gaps = 3/53 (5%) Frame = +2 Query: 242 PGGTAALGNPPGTPRPGFSMSRRGA---NGLNIIPPPKGENGSRSEWPTLRPG 391 PG G P P ++ ++G +GL +P P+GE G R +P RPG Sbjct: 207 PGTKGEKGEPARHPE-NYNKGQKGEPGNDGLEGLPGPQGEVGPRG-FPG-RPG 256 Score = 22.6 bits (46), Expect = 9.9 Identities = 14/41 (34%), Positives = 17/41 (41%) Frame = +2 Query: 248 GTAALGNPPGTPRPGFSMSRRGANGLNIIPPPKGENGSRSE 370 G A G P PG +G G +P KGE G + E Sbjct: 536 GDAKEGRPGAPGLPGRD-GEKGEPGRPGLPGAKGERGLKGE 575 >DQ303468-1|ABC18327.1| 1115|Anopheles gambiae putative methoprene-tolerant protein protein. Length = 1115 Score = 24.2 bits (50), Expect = 3.2 Identities = 11/20 (55%), Positives = 11/20 (55%) Frame = -3 Query: 396 VPPGRNVGHSDLDPFSPFGG 337 VPP N H PFSP GG Sbjct: 827 VPPLPNSQHYFTQPFSPSGG 846 >M93690-2|AAA29363.1| 1212|Anopheles gambiae unknown protein. Length = 1212 Score = 23.8 bits (49), Expect = 4.3 Identities = 7/21 (33%), Positives = 12/21 (57%) Frame = +1 Query: 262 WQSTWNTQAWVLNVSTWSKRV 324 WQS W+ +A + W+ R+ Sbjct: 944 WQSQWDAEADTSRYTRWTHRI 964 >AJ439398-8|CAD28131.1| 1283|Anopheles gambiae putative differentiation regulator protein. Length = 1283 Score = 23.8 bits (49), Expect = 4.3 Identities = 10/17 (58%), Positives = 10/17 (58%) Frame = +2 Query: 236 RAPGGTAALGNPPGTPR 286 R PG AA G PP T R Sbjct: 908 RGPGAAAATGPPPPTHR 924 >M93689-1|AAA29368.1| 442|Anopheles gambiae protein ( Anopheles gambiae T1 retroposon. ). Length = 442 Score = 22.6 bits (46), Expect = 9.9 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = -3 Query: 279 VPGGLPRAAVPPGARFDPFA 220 V GLP+ + ARFDP A Sbjct: 331 VDRGLPKQRIHERARFDPSA 350 >AJ439353-10|CAD27932.1| 3325|Anopheles gambiae F25C8.3 protein protein. Length = 3325 Score = 22.6 bits (46), Expect = 9.9 Identities = 8/22 (36%), Positives = 15/22 (68%) Frame = +2 Query: 74 CVIVYITLSHILQNTDYMNIFS 139 C++ Y+T + I +++ NIFS Sbjct: 2237 CIMRYVTATFINAASNFKNIFS 2258 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 615,458 Number of Sequences: 2352 Number of extensions: 13653 Number of successful extensions: 44 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 43 length of database: 563,979 effective HSP length: 61 effective length of database: 420,507 effective search space used: 57609459 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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