Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0019
(724 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic... 257 2e-67
UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor... 244 2e-63
UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;... 187 3e-46
UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=... 163 3e-39
UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor... 155 1e-36
UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor... 152 1e-35
UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative; ... 143 5e-33
UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor... 122 7e-27
UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release fa... 111 2e-23
UniRef50_P58227 Cluster: Peptide chain release factor subunit 1;... 103 3e-21
UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;... 96 9e-19
UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus ... 90 6e-17
UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2; Cr... 89 8e-17
UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;... 85 2e-15
UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;... 83 7e-15
UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-... 80 6e-14
UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:... 77 3e-13
UniRef50_Q46FF0 Cluster: Peptide chain release factor, subunit 1... 73 7e-12
UniRef50_Q9YAF1 Cluster: Peptide chain release factor subunit 1;... 66 6e-10
UniRef50_Q8ZU81 Cluster: Peptide chain release factor subunit 1;... 66 1e-09
UniRef50_Q3UUT4 Cluster: 16 days neonate thymus cDNA, RIKEN full... 35 2.3
UniRef50_A0CCA2 Cluster: Chromosome undetermined scaffold_167, w... 34 4.1
UniRef50_UPI00006CC094 Cluster: hypothetical protein TTHERM_0021... 33 7.1
>UniRef50_UPI0000DB724B Cluster: PREDICTED: similar to eukaryotic
release factor 1 CG5605-PA, isoform A; n=1; Apis
mellifera|Rep: PREDICTED: similar to eukaryotic release
factor 1 CG5605-PA, isoform A - Apis mellifera
Length = 265
Score = 257 bits (630), Expect = 2e-67
Identities = 124/126 (98%), Positives = 126/126 (100%)
Frame = +2
Query: 347 SEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNI 526
+EE+SADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNI
Sbjct: 3 NEETSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNI 62
Query: 527 KSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINT 706
KSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINT
Sbjct: 63 KSRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINT 122
Query: 707 SLYLCD 724
SLYLCD
Sbjct: 123 SLYLCD 128
>UniRef50_P62495 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=137; Eukaryota|Rep: Eukaryotic peptide
chain release factor subunit 1 - Homo sapiens (Human)
Length = 437
Score = 244 bits (597), Expect = 2e-63
Identities = 117/125 (93%), Positives = 121/125 (96%)
Frame = +2
Query: 350 EESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIK 529
+ S+ADRNVEIWKIKKLIKSLE ARGNGTSMISLIIPPKDQISRV+KMLADEFGTASNIK
Sbjct: 4 DPSAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIK 63
Query: 530 SRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTS 709
SRVNRLSVLGAITSVQ RLKLY KVPPNGLV+YCGTIVTEEGKEKKVNIDFEPFKPINTS
Sbjct: 64 SRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIVTEEGKEKKVNIDFEPFKPINTS 123
Query: 710 LYLCD 724
LYLCD
Sbjct: 124 LYLCD 128
>UniRef50_Q8SRZ6 Cluster: PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1;
n=1; Encephalitozoon cuniculi|Rep: PEPTIDE CHAIN RELEASE
FACTOR SUBUNIT 1 - Encephalitozoon cuniculi
Length = 386
Score = 187 bits (455), Expect = 3e-46
Identities = 83/117 (70%), Positives = 104/117 (88%)
Frame = +2
Query: 374 VEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSV 553
VE+W++KKL+++L +RGNGTSMIS+IIPPKDQISRVSKML DE+GTASNIKSRVNRLSV
Sbjct: 5 VEMWRMKKLLRNLRDSRGNGTSMISIIIPPKDQISRVSKMLTDEYGTASNIKSRVNRLSV 64
Query: 554 LGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
LGAITS Q +LK Y+K PPNGL I+ GT++T++ KEKK+++ EP KP+NTSLY+CD
Sbjct: 65 LGAITSAQSKLKQYSKTPPNGLGIFVGTVLTDQNKEKKISVGIEPIKPVNTSLYMCD 121
>UniRef50_Q98RP4 Cluster: Eukaryotic release factor 1 homolog; n=1;
Guillardia theta|Rep: Eukaryotic release factor 1
homolog - Guillardia theta (Cryptomonas phi)
Length = 409
Score = 163 bits (397), Expect = 3e-39
Identities = 74/116 (63%), Positives = 90/116 (77%)
Frame = +2
Query: 377 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVL 556
E+WKIKK + L +GNGTSMISL+IP + IS+VSKML+DE TASNIKSR+NR SVL
Sbjct: 9 ELWKIKKTYQYLSTIKGNGTSMISLVIPSNEHISKVSKMLSDELATASNIKSRINRSSVL 68
Query: 557 GAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
AI+SVQ +LK YTK P NGL+IYCGT E KEKK+ I+ EP KP+N+S Y+CD
Sbjct: 69 SAISSVQQKLKYYTKTPNNGLIIYCGTFTDENNKEKKILIEIEPLKPLNSSFYICD 124
>UniRef50_Q9NAX8 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=6; Trypanosomatidae|Rep: Eukaryotic peptide
chain release factor subunit 1 - Trypanosoma brucei
brucei
Length = 452
Score = 155 bits (376), Expect = 1e-36
Identities = 71/125 (56%), Positives = 97/125 (77%)
Frame = +2
Query: 350 EESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIK 529
E S +++ +E +K+KKLI+ LE ARG GTS+IS+ + PK+QIS + L +E+GTASNIK
Sbjct: 5 ELSESEKAIERYKVKKLIQMLESARGMGTSVISIYMTPKEQISGMVTKLNNEYGTASNIK 64
Query: 530 SRVNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTS 709
S N+LSV AIT+ RLK Y ++PPNGL++YCGT++T E KEKK+ +D EPFKP++ S
Sbjct: 65 SHTNKLSVQSAITASLGRLKQYNRLPPNGLLLYCGTVLTAENKEKKLTLDIEPFKPVSRS 124
Query: 710 LYLCD 724
LYLCD
Sbjct: 125 LYLCD 129
>UniRef50_Q9NCP1 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=2; Giardia intestinalis|Rep: Eukaryotic
peptide chain release factor subunit 1 - Giardia lamblia
(Giardia intestinalis)
Length = 457
Score = 152 bits (368), Expect = 1e-35
Identities = 69/120 (57%), Positives = 93/120 (77%)
Frame = +2
Query: 365 DRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNR 544
+R + IW++K+ I+ LE G+GTS+ISL+IPP +Q+S V+ ML +E+GTASNIKSRVN+
Sbjct: 15 ERQIAIWQLKRTIEKLESYTGSGTSVISLLIPPGEQLSSVTGMLTNEYGTASNIKSRVNK 74
Query: 545 LSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
+VL AITS RLKLY ++PPNGL +YCG I E+GK KV ID+ P+KPI+ +YLCD
Sbjct: 75 NAVLSAITSAMSRLKLYNRLPPNGLAVYCGEI-EEDGKNTKVVIDYTPYKPISNFIYLCD 133
>UniRef50_A2EPZ2 Cluster: Eukaryotic release factor 1, putative;
n=2; Trichomonas vaginalis|Rep: Eukaryotic release
factor 1, putative - Trichomonas vaginalis G3
Length = 438
Score = 143 bits (346), Expect = 5e-33
Identities = 65/117 (55%), Positives = 89/117 (76%)
Frame = +2
Query: 374 VEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSV 553
+E++KIK+LI L A G+GTSM++++IPP D + R+ + L DE GTASNIK+RVNR SV
Sbjct: 14 IELFKIKRLINYLRDAHGDGTSMVTILIPPGDAVHRMRQKLTDELGTASNIKNRVNRQSV 73
Query: 554 LGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
AIT+ +L + PPNGL ++CGTI+ EEGKEKK+ DFEPF+P++ SLY+CD
Sbjct: 74 EAAITAAIQKLGQFNSTPPNGLCVFCGTII-EEGKEKKLMKDFEPFRPLHQSLYICD 129
>UniRef50_Q5CD84 Cluster: Eukaryotic peptide chain release factor
subunit 1; n=5; Eukaryota|Rep: Eukaryotic peptide chain
release factor subunit 1 - Loxodes striatus
Length = 436
Score = 122 bits (295), Expect = 7e-27
Identities = 56/115 (48%), Positives = 83/115 (72%), Gaps = 1/115 (0%)
Frame = +2
Query: 383 WKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGA 562
WKI++LI+ LE A+GNGTS+ISLIIPPK+QIS +++ L D G A NIKS+ + +V A
Sbjct: 12 WKIRRLIEYLEKAKGNGTSLISLIIPPKEQISLINQKLTDAHGRAQNIKSKAVQKAVQDA 71
Query: 563 ITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGK-EKKVNIDFEPFKPINTSLYLCD 724
I S + +L + ++PPNGL++YCG + E+ K EK++ EP + INT+ +LC+
Sbjct: 72 IISTKQKLSVIKQIPPNGLILYCGKFIGEDEKTEKQILEVLEPLRAINTTFFLCE 126
>UniRef50_Q965E7 Cluster: Eukaryotic polypeptide chain release
factor 1; n=3; Paramecium tetraurelia|Rep: Eukaryotic
polypeptide chain release factor 1 - Paramecium
tetraurelia
Length = 437
Score = 111 bits (267), Expect = 2e-23
Identities = 53/124 (42%), Positives = 84/124 (67%), Gaps = 1/124 (0%)
Frame = +2
Query: 356 SSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSR 535
+ A+ +E +++KKLIK+L R GTS++S+ IPPK IS ++ L ++ A++IK +
Sbjct: 6 NDAEIALEQFRLKKLIKTLSQERTAGTSVVSVYIPPKRIISDITNRLNTQYAEAASIKDK 65
Query: 536 VNRLSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGK-EKKVNIDFEPFKPINTSL 712
NR+SV AI + RL+ Y K P NGLV++CG + +GK EKK+++ EP++P++ SL
Sbjct: 66 GNRISVQEAIQAAILRLRPYNKAPNNGLVVFCGIVQQADGKGEKKISVVIEPYRPLDLSL 125
Query: 713 YLCD 724
Y CD
Sbjct: 126 YFCD 129
>UniRef50_P58227 Cluster: Peptide chain release factor subunit 1;
n=5; Thermoplasmatales|Rep: Peptide chain release factor
subunit 1 - Thermoplasma volcanium
Length = 418
Score = 103 bits (248), Expect = 3e-21
Identities = 52/120 (43%), Positives = 70/120 (58%)
Frame = +2
Query: 365 DRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNR 544
D + ++ K+ ++ L G GT +ISL IPP+ QIS V L DE+ T+SNIKS+ R
Sbjct: 7 DEQIRRYEFKRALEELSKLHGRGTELISLYIPPEKQISDVVAYLRDEYSTSSNIKSKSTR 66
Query: 545 LSVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
+VL AI S+ RLK Y PPNGLV + G I T + + EP +PI T +Y CD
Sbjct: 67 KNVLAAIESIMSRLKYYRFPPPNGLVFFVGHIATRGDQTEMYTKIIEPPEPITTFMYKCD 126
>UniRef50_Q8U0J4 Cluster: Peptide chain release factor subunit 1;
n=5; Archaea|Rep: Peptide chain release factor subunit 1
- Pyrococcus furiosus
Length = 420
Score = 95.9 bits (228), Expect = 9e-19
Identities = 47/119 (39%), Positives = 69/119 (57%)
Frame = +2
Query: 368 RNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRL 547
R+ +++++KK I L+ RG GT +ISL IP +S+V + L +E+ TA NIKS+ R
Sbjct: 7 RDAQLYELKKKIDELKKIRGRGTELISLYIPAGYDLSKVMQQLREEYSTAQNIKSKTTRK 66
Query: 548 SVLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
+VLGA+ LKLY + P NGL ++ G + EG P +P+N LY CD
Sbjct: 67 NVLGALERAMQHLKLYKQTPENGLALFVGNVSEMEGVTDIRLWAIIPPEPLNVRLYRCD 125
>UniRef50_A7DMG3 Cluster: ERF1 domain 2 protein; n=1; Candidatus
Nitrosopumilus maritimus SCM1|Rep: ERF1 domain 2 protein
- Candidatus Nitrosopumilus maritimus SCM1
Length = 426
Score = 89.8 bits (213), Expect = 6e-17
Identities = 47/122 (38%), Positives = 71/122 (58%), Gaps = 4/122 (3%)
Frame = +2
Query: 371 NVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLS 550
+V+++KI+K ++ L G GT +I++ IP Q+ + L E GTA NIKS + R
Sbjct: 11 SVKVYKIRKTLEELSKKSGRGTELITVYIPKGKQLHEIIGTLQQEQGTADNIKSDLTRSH 70
Query: 551 VLGAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEG---KEKKVNI-DFEPFKPINTSLYL 718
V+ ++ V RLKLY K P GLV++CG + EEG + V + + +P K +N LY
Sbjct: 71 VVDSLGKVVQRLKLYKKTPERGLVMFCGALPPEEGGPLGSEVVKVWEIDPPKDLNQYLYR 130
Query: 719 CD 724
CD
Sbjct: 131 CD 132
>UniRef50_A0RX46 Cluster: Peptide chain release factor 1; n=2;
Crenarchaeota|Rep: Peptide chain release factor 1 -
Cenarchaeum symbiosum
Length = 411
Score = 89.4 bits (212), Expect = 8e-17
Identities = 46/119 (38%), Positives = 67/119 (56%), Gaps = 4/119 (3%)
Frame = +2
Query: 380 IWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLG 559
++KI+K++ L G GT +IS+ IP Q+ + +L +E GTA NIKS + R V+
Sbjct: 1 MYKIRKMLDELSGISGRGTELISVYIPKGKQLHEIISVLKEEQGTADNIKSDLTRTHVVD 60
Query: 560 AITSVQHRLKLYTKVPPNGLVIYCGTIVTE----EGKEKKVNIDFEPFKPINTSLYLCD 724
++ V RL+LY K P GLVI+CG + E G E + +P K + T LY CD
Sbjct: 61 SLAKVVQRLRLYKKTPERGLVIFCGALPPEGGGPTGSETVRAFEIDPPKELRTFLYRCD 119
>UniRef50_Q58239 Cluster: Peptide chain release factor subunit 1;
n=12; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Methanococcus jannaschii
Length = 421
Score = 85.0 bits (201), Expect = 2e-15
Identities = 46/117 (39%), Positives = 69/117 (58%), Gaps = 1/117 (0%)
Frame = +2
Query: 377 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVL 556
+++ KK++K L+ +G GT +ISL IP +IS V++ L +E ASNIKS+ R +V
Sbjct: 8 QLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQASNIKSKSTRKNVQ 67
Query: 557 GAITSVQHRLKLYTKVPPNGLVIYCGTIV-TEEGKEKKVNIDFEPFKPINTSLYLCD 724
AI ++ RLKL + G+VI+ G + + G EK EP +PI T +Y CD
Sbjct: 68 SAIEAILQRLKLLKEPLEKGVVIFAGMVPRSGPGTEKMETYVIEPPEPIKTYIYRCD 124
>UniRef50_Q9HNF0 Cluster: Peptide chain release factor subunit 1;
n=7; Euryarchaeota|Rep: Peptide chain release factor
subunit 1 - Halobacterium salinarium (Halobacterium
halobium)
Length = 416
Score = 83.0 bits (196), Expect = 7e-15
Identities = 47/131 (35%), Positives = 75/131 (57%), Gaps = 2/131 (1%)
Frame = +2
Query: 338 NKMSEESSADRNVEIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTA 517
++ E S DR ++ +K+I+ L+ G+GT ++++ IPP QIS V + E A
Sbjct: 2 SEQDEVPSEDRRK--YEFRKVIEELKDYEGSGTQLVTIYIPPDKQISDVVAHVTQEHSEA 59
Query: 518 SNIKSRVNRLSVLGAITSVQHRLKLYTKVPP-NGLVIYCGTIVTEEGKEKKVNIDFE-PF 691
SNIKS+ R +V A+TS++ RL+ Y PP NG+V++ G + + G+ V E P
Sbjct: 60 SNIKSKQTRTNVQDALTSIKDRLRYYDTFPPDNGMVVFSGAVDSGGGRTDMVTEVLESPP 119
Query: 692 KPINTSLYLCD 724
+PI + Y CD
Sbjct: 120 QPIESFRYHCD 130
>UniRef50_Q8TS00 Cluster: Peptide chain release factor subunit 1-2;
n=9; Euryarchaeota|Rep: Peptide chain release factor
subunit 1-2 - Methanosarcina acetivorans
Length = 415
Score = 79.8 bits (188), Expect = 6e-14
Identities = 44/116 (37%), Positives = 67/116 (57%)
Frame = +2
Query: 377 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVL 556
E + KK +++L+ G T +ISL IPP QIS V+K L +E ASNI S++ R +V
Sbjct: 8 EKYVFKKQLETLKSKNGRSTELISLYIPPDKQISDVTKHLREEHEQASNIISKLIRNNVQ 67
Query: 557 GAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
A+ S+ +L+ K+P NG+V + GT+ T + VN P +P+ +Y CD
Sbjct: 68 EALHSLLAKLRSLHKIPENGIVYFTGTVDTGANRTGMVNEVLFPPEPVADYIYRCD 123
>UniRef50_Q74N59 Cluster: NEQ052; n=1; Nanoarchaeum equitans|Rep:
NEQ052 - Nanoarchaeum equitans
Length = 358
Score = 77.4 bits (182), Expect = 3e-13
Identities = 41/116 (35%), Positives = 63/116 (54%)
Frame = +2
Query: 377 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVL 556
E +K+K L++ L+ G GT ++SL IP I ++ L E+ TA+NIK R +V
Sbjct: 5 EKYKLKSLLRQLKSIEGRGTELVSLYIPSNYPIGKILDQLNYEYATAANIKDDTTRKNVQ 64
Query: 557 GAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
A+ + + LK + K+P NGLVI+ G I ++ ++ P K N LY CD
Sbjct: 65 AALAKIINYLKDFKKIPENGLVIFAGNIAGRGDQDVRLWAIVPPEKS-NLKLYRCD 119
>UniRef50_Q46FF0 Cluster: Peptide chain release factor, subunit 1;
n=2; Methanosarcinaceae|Rep: Peptide chain release
factor, subunit 1 - Methanosarcina barkeri (strain
Fusaro / DSM 804)
Length = 415
Score = 72.9 bits (171), Expect = 7e-12
Identities = 41/116 (35%), Positives = 62/116 (53%)
Frame = +2
Query: 377 EIWKIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVL 556
E + KK +++L G T +ISL IP QIS V+ L +E AS IKS++ +V
Sbjct: 8 EKYVFKKKLETLRNKNGKSTELISLYIPSDKQISDVTNHLREEHEQASKIKSKLTSNNVQ 67
Query: 557 GAITSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
GA+ S+ +L+ K+P NG+V + G I T + V P +P+ +Y CD
Sbjct: 68 GALDSLLAKLRSLNKIPENGIVYFTGVIDTGANRTGMVTEVLIPPEPVVDYIYHCD 123
>UniRef50_Q9YAF1 Cluster: Peptide chain release factor subunit 1;
n=9; Thermoprotei|Rep: Peptide chain release factor
subunit 1 - Aeropyrum pernix
Length = 373
Score = 66.5 bits (155), Expect = 6e-10
Identities = 38/113 (33%), Positives = 61/113 (53%)
Frame = +2
Query: 386 KIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAI 565
++ +L+K L+ T ++SL IPP +S V +L E+ NIK + R +V A+
Sbjct: 16 ELARLLKELKKWSAPATVLLSLYIPPGRPLSDVMTLLRQEYSITDNIKLKRTRQAVKRAL 75
Query: 566 TSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
++ RL++ T PPNGLV++CG ++ GK + F P +PI Y D
Sbjct: 76 SAAMDRLQMLTSTPPNGLVLFCGEDMS-TGKFE--CFMFSPPEPIRVFYYRTD 125
>UniRef50_Q8ZU81 Cluster: Peptide chain release factor subunit 1;
n=5; Thermoproteaceae|Rep: Peptide chain release factor
subunit 1 - Pyrobaculum aerophilum
Length = 388
Score = 65.7 bits (153), Expect = 1e-09
Identities = 34/113 (30%), Positives = 59/113 (52%)
Frame = +2
Query: 386 KIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEFGTASNIKSRVNRLSVLGAI 565
+++ + L+ +G T++I+L I + I V +L E+ TA+NIK + R V +
Sbjct: 18 ELRAFVNLLKKFKGYATTLITLYINAERPIPDVVNLLRSEWSTAANIKDKTTRTHVQDTL 77
Query: 566 TSVQHRLKLYTKVPPNGLVIYCGTIVTEEGKEKKVNIDFEPFKPINTSLYLCD 724
+ + LK K P NG+ ++ G + +G + V P +PINT Y+CD
Sbjct: 78 ERIINNLKGEAKAPENGMAVFAGFHMINQGNYEWVYYVVIPPQPINTFKYICD 130
>UniRef50_Q3UUT4 Cluster: 16 days neonate thymus cDNA, RIKEN
full-length enriched library, clone:A130075O06
product:hypothetical protein, full insert sequence; n=1;
Mus musculus|Rep: 16 days neonate thymus cDNA, RIKEN
full-length enriched library, clone:A130075O06
product:hypothetical protein, full insert sequence - Mus
musculus (Mouse)
Length = 103
Score = 34.7 bits (76), Expect = 2.3
Identities = 13/22 (59%), Positives = 18/22 (81%)
Frame = +2
Query: 659 EKKVNIDFEPFKPINTSLYLCD 724
+KK +DFEPFKP++TSL + D
Sbjct: 28 KKKARVDFEPFKPMSTSLRVAD 49
>UniRef50_A0CCA2 Cluster: Chromosome undetermined scaffold_167,
whole genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_167,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 243
Score = 33.9 bits (74), Expect = 4.1
Identities = 21/62 (33%), Positives = 26/62 (41%)
Frame = -1
Query: 382 YFDISIGRRFFRHFVSLFI*LINHKHYICINN*NNVFAQHQNFK*TNTMEREPEFQFQTA 203
Y I R + FVS+F LI H C N+ NN F Q Q + + E F
Sbjct: 54 YVTIPCARSLSQIFVSVFFNLIYLFHIFCNNSINNYFQQMQYYYFSQYFRSHSEMHFNNL 113
Query: 202 LI 197
LI
Sbjct: 114 LI 115
>UniRef50_UPI00006CC094 Cluster: hypothetical protein
TTHERM_00215930; n=1; Tetrahymena thermophila SB210|Rep:
hypothetical protein TTHERM_00215930 - Tetrahymena
thermophila SB210
Length = 905
Score = 33.1 bits (72), Expect = 7.1
Identities = 17/41 (41%), Positives = 26/41 (63%)
Frame = +2
Query: 386 KIKKLIKSLEMARGNGTSMISLIIPPKDQISRVSKMLADEF 508
K++ LI+ L NGT+ +SL I K Q +++S+ L DEF
Sbjct: 182 KVEYLIEQLLEGSNNGTNTLSLKILQKQQANQLSQDLKDEF 222
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 699,898,591
Number of Sequences: 1657284
Number of extensions: 13827925
Number of successful extensions: 34303
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 33200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 34291
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 58677691418
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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