Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0016
(715 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q6NP10 Cluster: LD13390p; n=7; Eukaryota|Rep: LD13390p ... 363 2e-99
UniRef50_Q9UBR1 Cluster: Beta-ureidopropionase; n=42; root|Rep: ... 343 2e-93
UniRef50_UPI0000DC0724 Cluster: ureidopropionase, beta; n=1; Rat... 263 3e-69
UniRef50_A7SG03 Cluster: Predicted protein; n=1; Nematostella ve... 250 2e-65
UniRef50_Q5L031 Cluster: Beta-alanine synthase; n=19; Bacteria|R... 184 3e-45
UniRef50_O61697 Cluster: Putative beta-ureidopropionase; n=1; Ma... 170 3e-41
UniRef50_Q2S196 Cluster: Hydrolase, carbon-nitrogen family; n=1;... 99 9e-20
UniRef50_Q1IQA8 Cluster: Nitrilase/cyanide hydratase and apolipo... 96 7e-19
UniRef50_Q972L1 Cluster: 281aa long hypothetical beta-ureidoprop... 95 2e-18
UniRef50_A4J4S3 Cluster: Nitrilase/cyanide hydratase and apolipo... 94 3e-18
UniRef50_Q972X1 Cluster: 264aa long hypothetical beta-ureidoprop... 93 5e-18
UniRef50_A7I5W9 Cluster: Porphyromonas-type peptidyl-arginine de... 93 5e-18
UniRef50_Q97RA3 Cluster: Carbon-nitrogen hydrolase family protei... 93 8e-18
UniRef50_A6DKQ0 Cluster: Carbon-nitrogen hydrolase family protei... 91 2e-17
UniRef50_A4B9A7 Cluster: Probable hydratase; n=2; Bacteria|Rep: ... 89 8e-17
UniRef50_Q1GTC5 Cluster: Nitrilase/cyanide hydratase and apolipo... 87 3e-16
UniRef50_Q9ABL5 Cluster: Hydrolase, carbon-nitrogen family; n=13... 85 1e-15
UniRef50_Q89413 Cluster: A78R protein; n=6; Chlorovirus|Rep: A78... 83 5e-15
UniRef50_A6CCK5 Cluster: Putative uncharacterized protein; n=1; ... 82 1e-14
UniRef50_Q8VYF5 Cluster: N-carbamoylputrescine amidase; n=60; ce... 81 4e-14
UniRef50_Q9UYV8 Cluster: Beta ureidopropionase; n=4; Thermococca... 78 2e-13
UniRef50_A7I2D9 Cluster: Hydrolase, carbon-nitrogen family; n=1;... 78 2e-13
UniRef50_A0QWL8 Cluster: Carbon-nitrogen hydrolase family protei... 76 1e-12
UniRef50_A6QC56 Cluster: Hydrolase; n=2; Bacteria|Rep: Hydrolase... 74 3e-12
UniRef50_Q606Z9 Cluster: Hydrolase, carbon-nitrogen family; n=38... 74 4e-12
UniRef50_A6TPX2 Cluster: Nitrilase/cyanide hydratase and apolipo... 74 4e-12
UniRef50_Q2AH52 Cluster: Nitrilase/cyanide hydratase and apolipo... 73 5e-12
UniRef50_Q0AX54 Cluster: N-carbamoyl-D-amino acid amidohydrolase... 73 9e-12
UniRef50_Q0LQX0 Cluster: Nitrilase/cyanide hydratase and apolipo... 72 1e-11
UniRef50_A7GE66 Cluster: Hydrolase, carbon-nitrogen family; n=13... 71 3e-11
UniRef50_Q6AHZ8 Cluster: Putative uncharacterized protein DKFZp7... 70 5e-11
UniRef50_Q7M8G2 Cluster: HYDROLASE-Predicted amidohydrolase; n=5... 69 9e-11
UniRef50_O30121 Cluster: Putative uncharacterized protein; n=1; ... 69 9e-11
UniRef50_Q183H2 Cluster: Putative carbon-nitrogen hydrolase; n=2... 69 2e-10
UniRef50_A6DDT2 Cluster: HYDROLASE-Predicted amidohydrolase; n=1... 68 2e-10
UniRef50_A6BCC3 Cluster: Carbon-nitrogen hydrolase family protei... 68 3e-10
UniRef50_A4M5M1 Cluster: Nitrilase/cyanide hydratase and apolipo... 66 8e-10
UniRef50_A5C5V4 Cluster: Putative uncharacterized protein; n=1; ... 66 8e-10
UniRef50_O59829 Cluster: Nitrilase; n=2; cellular organisms|Rep:... 66 8e-10
UniRef50_A4J6K3 Cluster: Nitrilase/cyanide hydratase and apolipo... 64 2e-09
UniRef50_Q8W0T9 Cluster: Putative uncharacterized protein SB35P0... 64 2e-09
UniRef50_Q8RUF8 Cluster: AT5g12040/F14F18_210; n=9; Magnoliophyt... 64 2e-09
UniRef50_Q54JM9 Cluster: Putative uncharacterized protein; n=1; ... 64 3e-09
UniRef50_Q2NHR0 Cluster: Predicted amidohydrolase; n=1; Methanos... 64 3e-09
UniRef50_A3H7D3 Cluster: Nitrilase/cyanide hydratase and apolipo... 64 4e-09
UniRef50_Q6N746 Cluster: Nitrilase/cyanide hydratase and apolipo... 63 6e-09
UniRef50_A6TL48 Cluster: Nitrilase/cyanide hydratase and apolipo... 63 6e-09
UniRef50_A1HQ26 Cluster: Nitrilase/cyanide hydratase and apolipo... 63 6e-09
UniRef50_A6CCB9 Cluster: Predicted amidohydrolase; n=1; Planctom... 63 8e-09
UniRef50_A7DPX6 Cluster: Nitrilase/cyanide hydratase and apolipo... 63 8e-09
UniRef50_Q74H63 Cluster: Hydrolase, carbon-nitrogen family; n=8;... 61 2e-08
UniRef50_Q44185 Cluster: N-carbamoyl-D-amino acid hydrolase; n=1... 61 2e-08
UniRef50_A2BNC1 Cluster: Predicted amidohydrolase; n=1; Hyperthe... 60 4e-08
UniRef50_Q4P4D1 Cluster: Putative uncharacterized protein; n=1; ... 60 5e-08
UniRef50_A0TTW8 Cluster: Nitrilase/cyanide hydratase and apolipo... 60 7e-08
UniRef50_A2XD42 Cluster: Putative uncharacterized protein; n=2; ... 60 7e-08
UniRef50_Q6TGW8 Cluster: Nit protein 2; n=22; Fungi/Metazoa grou... 59 1e-07
UniRef50_Q0RPB5 Cluster: Putative methylthioribose recycling pro... 59 1e-07
UniRef50_A0BLB1 Cluster: Chromosome undetermined scaffold_114, w... 58 2e-07
UniRef50_A4SZC4 Cluster: Nitrilase/cyanide hydratase and apolipo... 58 2e-07
UniRef50_Q6L0F7 Cluster: Carbon-nitrogen hydrolase family; n=2; ... 58 2e-07
UniRef50_Q8ZVX6 Cluster: Nitrilase, conjectural; n=4; Pyrobaculu... 58 3e-07
UniRef50_Q1QTM0 Cluster: Nitrilase/cyanide hydratase and apolipo... 57 4e-07
UniRef50_Q1AWK1 Cluster: Nitrilase/cyanide hydratase and apolipo... 57 4e-07
UniRef50_A1HPP3 Cluster: Nitrilase/cyanide hydratase and apolipo... 57 4e-07
UniRef50_Q5KJU9 Cluster: Hydrolase, putative; n=1; Filobasidiell... 56 7e-07
UniRef50_A5FKF8 Cluster: Nitrilase/cyanide hydratase and apolipo... 56 9e-07
UniRef50_Q8WUF0 Cluster: Nitrilase family member 2; n=28; cellul... 56 1e-06
UniRef50_UPI0000E1FE2F Cluster: PREDICTED: similar to Nitrilase ... 55 2e-06
UniRef50_Q9KE11 Cluster: BH1047 protein; n=1; Bacillus haloduran... 55 2e-06
UniRef50_A5V6Z2 Cluster: Nitrilase/cyanide hydratase and apolipo... 55 2e-06
UniRef50_UPI000051A529 Cluster: PREDICTED: similar to Nitrilase ... 55 2e-06
UniRef50_A3TQB8 Cluster: Nitrilase/cyanide hydratase and apolipo... 55 2e-06
UniRef50_A2BKF1 Cluster: Predicted amidohydrolase; n=1; Hyperthe... 55 2e-06
UniRef50_Q2JDM2 Cluster: Nitrilase/cyanide hydratase and apolipo... 54 3e-06
UniRef50_A1IFF1 Cluster: Hydrolase, carbon-nitrogen family; n=1;... 54 3e-06
UniRef50_A0RYH6 Cluster: Amidohydrolase; n=1; Cenarchaeum symbio... 54 3e-06
UniRef50_Q1PXD4 Cluster: Similar to N-carbamoyl-D-amino acid hyd... 54 5e-06
UniRef50_A0QPL8 Cluster: Hydrolase, carbon-nitrogen family prote... 54 5e-06
UniRef50_Q6N4F1 Cluster: Possible amidohydrolase; n=2; Rhodopseu... 53 6e-06
UniRef50_Q2S2E4 Cluster: Hydrolase, carbon-nitrogen family; n=1;... 53 6e-06
UniRef50_A4SSL0 Cluster: Beta-ureidopropionase; n=1; Aeromonas s... 53 8e-06
UniRef50_Q0S9Y1 Cluster: Possible nitrilase; n=4; Actinomycetale... 52 1e-05
UniRef50_A3LZY2 Cluster: Aliphatic nitrilase; n=1; Pichia stipit... 52 1e-05
UniRef50_A4WA35 Cluster: Nitrilase/cyanide hydratase and apolipo... 52 1e-05
UniRef50_A4BQN0 Cluster: Nitrilase/cyanide hydratase and apolipo... 52 1e-05
UniRef50_A3ZLM3 Cluster: Putative nitrilase; n=1; Blastopirellul... 52 1e-05
UniRef50_A0L7H1 Cluster: Nitrilase/cyanide hydratase and apolipo... 52 1e-05
UniRef50_Q17CS4 Cluster: Nitrilase, putative; n=3; Culicidae|Rep... 52 1e-05
UniRef50_Q72HE8 Cluster: Beta-ureidopropionase; n=2; Thermus the... 52 2e-05
UniRef50_P46011 Cluster: Nitrilase 4; n=49; cellular organisms|R... 52 2e-05
UniRef50_Q9ZMC7 Cluster: Putative; n=6; Campylobacterales|Rep: P... 51 2e-05
UniRef50_Q8DCG5 Cluster: Predicted amidohydrolase; n=33; Gammapr... 51 2e-05
UniRef50_Q18UU7 Cluster: Nitrilase/cyanide hydratase and apolipo... 51 2e-05
UniRef50_A5D6C3 Cluster: Putative uncharacterized protein; n=1; ... 51 2e-05
UniRef50_Q5WM18 Cluster: Methylthioribose recycling protein; n=2... 51 3e-05
UniRef50_Q2LUZ0 Cluster: Carbon-nitrogen hydrolase family protei... 51 3e-05
UniRef50_Q1F028 Cluster: Nitrilase/cyanide hydratase and apolipo... 51 3e-05
UniRef50_Q47VH0 Cluster: Hydrolase, carbon-nitrogen family; n=1;... 50 4e-05
UniRef50_A4EPU1 Cluster: Putative hydrolase; n=2; Rhodobacterace... 50 4e-05
UniRef50_P58054 Cluster: UPF0012 hydrolase ybeM; n=33; Proteobac... 50 4e-05
UniRef50_Q86X76 Cluster: Nitrilase homolog 1; n=29; Eumetazoa|Re... 50 4e-05
UniRef50_Q84FR7 Cluster: D-N-carbamoylase; n=1; Arthrobacter cry... 50 6e-05
UniRef50_Q6JHR5 Cluster: Aliphatic amidase; n=1; Saccharopolyspo... 50 8e-05
UniRef50_A3CTE8 Cluster: Nitrilase/cyanide hydratase and apolipo... 50 8e-05
UniRef50_A3EVA0 Cluster: NAD synthase; n=4; Bacteria|Rep: NAD sy... 49 1e-04
UniRef50_Q9HIW8 Cluster: Nitrilase related protein; n=2; Thermop... 49 1e-04
UniRef50_Q3W243 Cluster: GCN5-related N-acetyltransferase:AIR sy... 49 1e-04
UniRef50_A5TTZ3 Cluster: Possible amidohydrolase; n=1; Fusobacte... 49 1e-04
UniRef50_A1S062 Cluster: Nitrilase/cyanide hydratase and apolipo... 49 1e-04
UniRef50_Q1LPP8 Cluster: Nitrilase/cyanide hydratase and apolipo... 48 2e-04
UniRef50_Q2QQ94 Cluster: Hydrolase, carbon-nitrogen family prote... 48 2e-04
UniRef50_P55175 Cluster: UPF0012 hydrolase sll0601; n=40; Cyanob... 48 2e-04
UniRef50_Q6RWQ0 Cluster: Nitrilase; n=3; uncultured organism|Rep... 48 2e-04
UniRef50_A0JSY8 Cluster: Nitrilase/cyanide hydratase and apolipo... 48 2e-04
UniRef50_A0BR54 Cluster: Chromosome undetermined scaffold_122, w... 48 2e-04
UniRef50_Q3A0A3 Cluster: Predicted amidohydrolase; n=1; Pelobact... 48 3e-04
UniRef50_UPI0000D55F17 Cluster: PREDICTED: similar to CG7067-PA;... 47 5e-04
UniRef50_Q0HEI5 Cluster: Nitrilase/cyanide hydratase and apolipo... 47 5e-04
UniRef50_A5WCY0 Cluster: Nitrilase/cyanide hydratase and apolipo... 47 5e-04
UniRef50_A4XN12 Cluster: Nitrilase/cyanide hydratase and apolipo... 47 5e-04
UniRef50_A0U0W3 Cluster: Nitrilase/cyanide hydratase and apolipo... 47 5e-04
UniRef50_Q3IW15 Cluster: Predicted amidohydrolase; n=2; Rhodobac... 46 7e-04
UniRef50_Q75TH8 Cluster: Putative uncharacterized protein GSB07;... 46 7e-04
UniRef50_A1SU00 Cluster: Nitrilase/cyanide hydratase and apolipo... 46 7e-04
UniRef50_A0R400 Cluster: Hydrolase, carbon-nitrogen family prote... 46 7e-04
UniRef50_A0LH50 Cluster: Nitrilase/cyanide hydratase and apolipo... 46 7e-04
UniRef50_Q8FM85 Cluster: Putative uncharacterized protein; n=2; ... 46 0.001
UniRef50_A4BGL8 Cluster: Predicted amidohydrolase; n=1; Reinekea... 46 0.001
UniRef50_Q5DC61 Cluster: SJCHGC06938 protein; n=1; Schistosoma j... 46 0.001
UniRef50_Q5C342 Cluster: SJCHGC04680 protein; n=1; Schistosoma j... 46 0.001
UniRef50_Q5R0H6 Cluster: Predicted amidohydrolase, nitrilase fam... 46 0.001
UniRef50_Q2NTW0 Cluster: Putative uncharacterized protein; n=2; ... 46 0.001
UniRef50_Q4Q8W4 Cluster: Nitrilase, putative; n=6; Trypanosomati... 46 0.001
UniRef50_A6Q8M5 Cluster: Carbon-nitrogen hydrolase family protei... 45 0.002
UniRef50_A5GU42 Cluster: Nitrilase-related protein; n=1; Synecho... 45 0.002
UniRef50_A3Y529 Cluster: Putative uncharacterized protein; n=1; ... 45 0.002
UniRef50_Q4WEA8 Cluster: Hydrolase, carbon-nitrogen family, puta... 45 0.002
UniRef50_Q7UWX1 Cluster: Beta-alanine synthetase; n=1; Pirellula... 45 0.002
UniRef50_Q18UY7 Cluster: Nitrilase/cyanide hydratase and apolipo... 45 0.002
UniRef50_Q9LE50 Cluster: Nitrilase 1 like protein; n=2; Arabidop... 45 0.002
UniRef50_Q60BT4 Cluster: Hydrolase, carbon-nitrogen family; n=15... 44 0.003
UniRef50_Q0LC17 Cluster: NAD+ synthetase; n=1; Herpetosiphon aur... 44 0.003
UniRef50_A6X6J7 Cluster: Nitrilase/cyanide hydratase and apolipo... 44 0.003
UniRef50_A5G317 Cluster: Nitrilase/cyanide hydratase and apolipo... 44 0.003
UniRef50_A0JW88 Cluster: Nitrilase/cyanide hydratase and apolipo... 44 0.003
UniRef50_P55176 Cluster: UPF0012 hydrolase in pqqF 5'region; n=1... 44 0.003
UniRef50_A7FDR9 Cluster: Hydrolase, carbon-nitrogen family prote... 44 0.004
UniRef50_A0LQU6 Cluster: Nitrilase/cyanide hydratase and apolipo... 44 0.004
UniRef50_UPI0000E472D9 Cluster: PREDICTED: similar to Ureidoprop... 44 0.005
UniRef50_Q89E80 Cluster: Bll7207 protein; n=1; Bradyrhizobium ja... 44 0.005
UniRef50_Q28TG7 Cluster: Nitrilase/cyanide hydratase and apolipo... 44 0.005
UniRef50_Q127K6 Cluster: Nitrilase/cyanide hydratase and apolipo... 44 0.005
UniRef50_Q0SBF1 Cluster: Probable nitrilase; n=2; Actinomycetale... 44 0.005
UniRef50_A6DN63 Cluster: Nitrilase/cyanide hydratase and apolipo... 44 0.005
UniRef50_P47016 Cluster: Probable hydrolase NIT2; n=6; Saccharom... 44 0.005
UniRef50_O76463 Cluster: Nitrilase and fragile histidine triad f... 44 0.005
UniRef50_Q15ZG7 Cluster: Nitrilase/cyanide hydratase and apolipo... 43 0.007
UniRef50_Q5KLT5 Cluster: Nitrilase-like protein, putative; n=2; ... 43 0.007
UniRef50_Q5K7Z3 Cluster: Expressed protein; n=1; Filobasidiella ... 43 0.007
UniRef50_Q6KZW3 Cluster: Carbon-nitrogen hydrolase; n=1; Picroph... 43 0.007
UniRef50_O25836 Cluster: Formamidase; n=17; Bacteria|Rep: Formam... 43 0.007
UniRef50_Q0S9R8 Cluster: Probable formamidase; n=1; Rhodococcus ... 43 0.009
UniRef50_A3H5Q5 Cluster: Nitrilase/cyanide hydratase and apolipo... 43 0.009
UniRef50_Q88EJ9 Cluster: Carbon-nitrogen hydrolase family protei... 42 0.011
UniRef50_A6T0X3 Cluster: Nitrilase; n=7; Bacteria|Rep: Nitrilase... 42 0.011
UniRef50_A4YSE7 Cluster: N-carbamoyl-D-amino acid hydrolase; n=1... 42 0.011
UniRef50_A3JKT7 Cluster: Acetyltransferase domain (GNAT family) ... 42 0.011
UniRef50_A5AAF3 Cluster: Contig An02c0310, complete genome; n=5;... 42 0.011
UniRef50_Q6RWN7 Cluster: Nitrilase; n=21; root|Rep: Nitrilase - ... 42 0.015
UniRef50_A6DBX4 Cluster: Nitrilase/cyanide hydratase and apolipo... 42 0.015
UniRef50_Q00Y86 Cluster: Carbon-nitrogen hydrolase; n=2; Ostreoc... 42 0.015
UniRef50_A1RZK0 Cluster: Nitrilase/cyanide hydratase and apolipo... 42 0.015
UniRef50_A7I462 Cluster: Hydrolase in agr operon; n=1; Campyloba... 42 0.020
UniRef50_A5FWH4 Cluster: Nitrilase/cyanide hydratase and apolipo... 42 0.020
UniRef50_A1SE99 Cluster: Nitrilase/cyanide hydratase and apolipo... 42 0.020
UniRef50_Q6C005 Cluster: Similar to sp|P47016 Saccharomyces cere... 42 0.020
UniRef50_Q9V1L5 Cluster: Amidohydrolase, putative; n=2; Thermoco... 42 0.020
UniRef50_Q46AW4 Cluster: Putative amidohydrolase; n=1; Methanosa... 42 0.020
UniRef50_A6CFF3 Cluster: Putative nitrilase; n=1; Planctomyces m... 41 0.026
UniRef50_A4R649 Cluster: Putative uncharacterized protein; n=1; ... 41 0.026
UniRef50_P54608 Cluster: UPF0012 hydrolase yhcX; n=12; Bacteria|... 41 0.026
UniRef50_O66508 Cluster: Putative uncharacterized protein; n=1; ... 41 0.035
UniRef50_A4YP30 Cluster: N-carbamoyl-D-amino acid hydrolase; n=4... 41 0.035
UniRef50_Q4P7D2 Cluster: Putative uncharacterized protein; n=1; ... 41 0.035
UniRef50_A7I641 Cluster: Nitrilase/cyanide hydratase and apolipo... 41 0.035
UniRef50_Q6RWR2 Cluster: Nitrilase; n=1; uncultured organism|Rep... 40 0.046
UniRef50_Q8KFB2 Cluster: Carbon-nitrogen hydrolase family protei... 40 0.046
UniRef50_Q89XU5 Cluster: Amidohydrolase; n=48; Alphaproteobacter... 40 0.046
UniRef50_Q4KB18 Cluster: Hydrolase, carbon-nitrogen family; n=2;... 40 0.061
UniRef50_A4XAH8 Cluster: Nitrilase/cyanide hydratase and apolipo... 40 0.061
UniRef50_A0J1U1 Cluster: Nitrilase/cyanide hydratase and apolipo... 40 0.061
UniRef50_Q1AZG5 Cluster: Nitrilase; n=1; Rubrobacter xylanophilu... 40 0.080
UniRef50_A5UTD2 Cluster: Nitrilase/cyanide hydratase and apolipo... 40 0.080
UniRef50_Q8KCC8 Cluster: Carbon-nitrogen hydrolase family protei... 39 0.11
UniRef50_Q16A64 Cluster: Hydrolase, putative; n=1; Roseobacter d... 39 0.11
UniRef50_A5V962 Cluster: Nitrilase/cyanide hydratase and apolipo... 39 0.11
UniRef50_A2ICY3 Cluster: Cyanide hydratase; n=23; Gammaproteobac... 39 0.11
UniRef50_UPI0000E105FE Cluster: putative hydrolase, carbon-nitro... 39 0.14
UniRef50_Q7URE5 Cluster: Predicted amidohydrolase; n=1; Pirellul... 39 0.14
UniRef50_A3M2Z7 Cluster: Putative glutamine-dependent NAD(+) syn... 39 0.14
UniRef50_Q7QAW0 Cluster: ENSANGP00000011026; n=2; Culicidae|Rep:... 39 0.14
UniRef50_Q6RWE5 Cluster: Nitrilase; n=4; root|Rep: Nitrilase - u... 38 0.19
UniRef50_Q8Y8V0 Cluster: Lmo0792 protein; n=12; Listeria|Rep: Lm... 38 0.19
UniRef50_Q2BKP4 Cluster: Putative carbon-nitrogen hydrolase; n=1... 38 0.19
UniRef50_Q12DE7 Cluster: Nitrilase/cyanide hydratase and apolipo... 38 0.19
UniRef50_A6T2L9 Cluster: Nitrilase; n=1; Janthinobacterium sp. M... 38 0.19
UniRef50_A4U2A6 Cluster: Nitrilase/cyanide hydratase and apolipo... 38 0.19
UniRef50_Q74FF8 Cluster: Hydrolase, carbon-nitrogen family; n=6;... 38 0.25
UniRef50_Q0VS65 Cluster: Carbon-nitrogen hydrolase family protei... 38 0.25
UniRef50_A3JK79 Cluster: Predicted amidohydrolase; n=3; Gammapro... 38 0.25
UniRef50_A2R283 Cluster: Contig An13c0120, complete genome; n=2;... 38 0.25
UniRef50_A1CIE7 Cluster: Hydrolase, carbon-nitrogen family prote... 38 0.25
UniRef50_UPI0000DAE70E Cluster: hypothetical protein Rgryl_01001... 38 0.32
UniRef50_UPI000023E628 Cluster: hypothetical protein FG00821.1; ... 38 0.32
UniRef50_Q2RGR0 Cluster: Nitrilase/cyanide hydratase and apolipo... 38 0.32
UniRef50_Q1MFH8 Cluster: Putative hydrolase; n=1; Rhizobium legu... 38 0.32
UniRef50_A1IFV0 Cluster: Putative hydrolase; n=1; Candidatus Des... 38 0.32
UniRef50_A6SN02 Cluster: Nitrilase; n=3; Sclerotiniaceae|Rep: Ni... 38 0.32
UniRef50_A3HXT3 Cluster: Putative nitrilase; n=1; Algoriphagus s... 37 0.43
UniRef50_A0NZI0 Cluster: Nitrilase/cyanide hydratase and apolipo... 37 0.43
UniRef50_A3LY98 Cluster: Nitrilase superfamily member; n=3; Sacc... 37 0.43
UniRef50_Q92DM8 Cluster: Lin0785 protein; n=5; Bacteria|Rep: Lin... 37 0.57
UniRef50_Q8KFP8 Cluster: Carbon-nitrogen hydrolase family protei... 37 0.57
UniRef50_Q1FPL1 Cluster: Nitrilase/cyanide hydratase and apolipo... 37 0.57
UniRef50_Q9PC24 Cluster: Probable glutamine-dependent NAD(+) syn... 37 0.57
UniRef50_Q5NN79 Cluster: Nitrilase; n=17; Proteobacteria|Rep: Ni... 36 0.75
UniRef50_Q2SQI0 Cluster: Predicted amidohydrolase; n=1; Hahella ... 36 0.75
UniRef50_O94660 Cluster: Nitrilase; n=6; Ascomycota|Rep: Nitrila... 36 0.75
UniRef50_A6UC57 Cluster: Nitrilase/cyanide hydratase and apolipo... 36 0.99
UniRef50_A6GKJ0 Cluster: Putative uncharacterized protein; n=1; ... 36 0.99
UniRef50_Q5V604 Cluster: Nitrilase; n=2; Halobacteriaceae|Rep: N... 36 0.99
UniRef50_O76464 Cluster: Nitrilase and fragile histidine triad f... 36 0.99
UniRef50_Q1GRP3 Cluster: Nitrilase/cyanide hydratase and apolipo... 36 1.3
UniRef50_Q0EPQ3 Cluster: Nitrilase/cyanide hydratase and apolipo... 36 1.3
UniRef50_A7DA57 Cluster: Nitrilase/cyanide hydratase and apolipo... 36 1.3
UniRef50_A3SP65 Cluster: Possible nitrilase; n=2; Rhodobacterace... 36 1.3
UniRef50_A1ZR32 Cluster: Hydrolase, carbon-nitrogen family; n=2;... 36 1.3
UniRef50_A0Y2B3 Cluster: Putative hydrolase, carbon-nitrogen fam... 36 1.3
UniRef50_A0M3E2 Cluster: Carbon-nitrogen hydrolase; n=6; cellula... 36 1.3
UniRef50_A0CJZ7 Cluster: Chromosome undetermined scaffold_2, who... 36 1.3
UniRef50_Q5AHS1 Cluster: Potential N-terminal amidase; n=2; Cand... 36 1.3
UniRef50_Q8TLM7 Cluster: Carbon-nitrogen hydrolase; n=2; Methano... 36 1.3
UniRef50_UPI000023E394 Cluster: hypothetical protein FG01991.1; ... 35 1.7
UniRef50_Q11M91 Cluster: Nitrilase/cyanide hydratase and apolipo... 35 1.7
UniRef50_A1HU09 Cluster: Nitrilase/cyanide hydratase and apolipo... 35 1.7
UniRef50_A1HNR2 Cluster: Nitrilase/cyanide hydratase and apolipo... 35 1.7
UniRef50_A0H2F4 Cluster: Nitrilase/cyanide hydratase and apolipo... 35 1.7
UniRef50_Q9X0Y0 Cluster: Probable glutamine-dependent NAD(+) syn... 35 1.7
UniRef50_Q6RWG5 Cluster: Nitrilase; n=2; uncultured organism|Rep... 35 2.3
UniRef50_Q5PMN3 Cluster: Possible hydrolase; n=4; Salmonella|Rep... 35 2.3
UniRef50_Q2CBA1 Cluster: Putative amidohydrolase; n=1; Oceanicol... 35 2.3
UniRef50_Q04W18 Cluster: Amidohydrolase; n=4; Leptospira|Rep: Am... 35 2.3
UniRef50_A6F4Z1 Cluster: Predicted amidohydrolase; n=4; Gammapro... 35 2.3
UniRef50_P40447 Cluster: Putative nitrilase-like protein NIT1; n... 35 2.3
UniRef50_UPI00015A73E8 Cluster: UPI00015A73E8 related cluster; n... 34 3.0
UniRef50_A6FJ09 Cluster: Putative hydrolase; n=1; Moritella sp. ... 34 3.0
UniRef50_Q483K8 Cluster: Hydrolase, carbon-nitrogen family; n=1;... 34 4.0
UniRef50_Q2ADS5 Cluster: Nitrilase/cyanide hydratase and apolipo... 34 4.0
UniRef50_Q8TPH5 Cluster: Carbon-nitrogen hydrolase; n=1; Methano... 34 4.0
UniRef50_P82605 Cluster: Nitrilase; n=4; Bacteria|Rep: Nitrilase... 34 4.0
UniRef50_Q7MQY7 Cluster: Putative uncharacterized protein; n=1; ... 33 5.3
UniRef50_Q5LLB2 Cluster: Nitrilase family protein; n=7; Bacteria... 33 5.3
UniRef50_P72907 Cluster: Slr1071 protein; n=1; Synechocystis sp.... 33 5.3
UniRef50_A4AR83 Cluster: Apolipoprotein N-acyltransferase; n=1; ... 33 5.3
UniRef50_A4R5F2 Cluster: Putative uncharacterized protein; n=1; ... 33 5.3
UniRef50_Q6RWQ5 Cluster: Nitrilase; n=1; uncultured organism|Rep... 33 7.0
UniRef50_Q3A713 Cluster: Predicted amidohydrolase family protein... 33 7.0
UniRef50_A4M7Y7 Cluster: Nitrilase/cyanide hydratase and apolipo... 33 7.0
UniRef50_Q6QDB8 Cluster: NIT4; n=2; Eukaryota|Rep: NIT4 - Vicia ... 33 7.0
UniRef50_Q871X8 Cluster: Related to amino-terminal amidase; n=1;... 33 7.0
UniRef50_Q2TYD8 Cluster: Carbon-nitrogen hydrolase; n=1; Aspergi... 33 7.0
UniRef50_UPI0000F20135 Cluster: PREDICTED: hypothetical protein;... 33 9.2
UniRef50_Q4SXH0 Cluster: Chromosome undetermined SCAF12413, whol... 33 9.2
UniRef50_Q9ADI8 Cluster: NAD(+) synthase; n=12; Bacteria|Rep: NA... 33 9.2
UniRef50_Q4HN14 Cluster: Hydrolase, carbon-nitrogen family; n=2;... 33 9.2
UniRef50_A0Q650 Cluster: Carbon-nitrogen hydrolase family protei... 33 9.2
UniRef50_Q23384 Cluster: Putative uncharacterized protein nit-1;... 33 9.2
UniRef50_Q2TX19 Cluster: Predicted protein; n=1; Aspergillus ory... 33 9.2
>UniRef50_Q6NP10 Cluster: LD13390p; n=7; Eukaryota|Rep: LD13390p -
Drosophila melanogaster (Fruit fly)
Length = 408
Score = 363 bits (894), Expect = 2e-99
Identities = 166/237 (70%), Positives = 193/237 (81%)
Frame = +3
Query: 3 FEIKAYDFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQ 182
F+IK Y F AR+E+ RK RIVR+G IQ+SI I T PI +QR AI+ KV+ +I AAA
Sbjct: 75 FDIKGYRFTAREEQTRKRRIVRVGAIQNSIVIPTTAPIEKQREAIWNKVKTMIKAAAEAG 134
Query: 183 VNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDD 362
NI+C QEAW MPFAFCTREK PWC+FAE GP+T LAELA Y+MVII ILERD
Sbjct: 135 CNIVCTQEAWTMPFAFCTREKFPWCEFAEEAENGPTTKMLAELAKAYNMVIIHSILERDM 194
Query: 363 IHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAIN 542
HG+TIWNTAVVI+ G+ +GKHRKNHIPRVGDFNESTYY EGNTGHPVFET++GK+A+N
Sbjct: 195 EHGETIWNTAVVISNSGRYLGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETEFGKLAVN 254
Query: 543 ICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
ICYGRHHP NW+MFG+NGAEIVFNPSAT+ LSE LW++EARNAAIANSY+T INR
Sbjct: 255 ICYGRHHPQNWMMFGLNGAEIVFNPSATIGRLSEPLWSIEARNAAIANSYFTVPINR 311
>UniRef50_Q9UBR1 Cluster: Beta-ureidopropionase; n=42; root|Rep:
Beta-ureidopropionase - Homo sapiens (Human)
Length = 384
Score = 343 bits (844), Expect = 2e-93
Identities = 148/237 (62%), Positives = 186/237 (78%)
Frame = +3
Query: 3 FEIKAYDFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQ 182
FE++ Y F A +E+ R+PRIV +GL+Q+ I + + P+ +Q A+ +++ I+ AA
Sbjct: 52 FELQGYAFEAAEEQLRRPRIVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCG 111
Query: 183 VNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDD 362
VNI+C QEAW MPFAFCTREK PW +FAE GP+T F +LA +DMV++SPILERD
Sbjct: 112 VNIICFQEAWTMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAKNHDMVVVSPILERDS 171
Query: 363 IHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAIN 542
HGD +WNTAVVI+ G V+GK RKNHIPRVGDFNESTYY EGN GHPVF+T++G++A+N
Sbjct: 172 EHGDVLWNTAVVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNLGHPVFQTQFGRIAVN 231
Query: 543 ICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
ICYGRHHPLNWLM+ INGAEI+FNPSAT+ LSE LW +EARNAAIAN +TCAINR
Sbjct: 232 ICYGRHHPLNWLMYSINGAEIIFNPSATIGALSESLWPIEARNAAIANHCFTCAINR 288
>UniRef50_UPI0000DC0724 Cluster: ureidopropionase, beta; n=1; Rattus
norvegicus|Rep: ureidopropionase, beta - Rattus
norvegicus
Length = 392
Score = 263 bits (644), Expect = 3e-69
Identities = 119/237 (50%), Positives = 165/237 (69%)
Frame = +3
Query: 3 FEIKAYDFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQ 182
FE+K Y F A KE+ R P+IVR+GL+Q+ I + T P+ +Q A+ +++++I AA
Sbjct: 52 FELKGYAFGAAKEQQRCPQIVRVGLVQNRIPLPTSAPVAEQVSALHKRIEEIAEVAAMCG 111
Query: 183 VNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDD 362
VNI+C QEAWNMPFAFCTREK PW +FAE G +T F + ++ + +I+ L +
Sbjct: 112 VNIICFQEAWNMPFAFCTREKLPWTEFAESAEDGLTTRFCQKGKFQHIVCLIAIFLRQSL 171
Query: 363 IHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAIN 542
G WN+ + G V + + H P + D++ STYY EGN GHPVF+T++G++A+N
Sbjct: 172 TLGLVAWNSLDISVNAGLVNARFKDVHHPVI-DYSYSTYYMEGNLGHPVFQTQFGRIAVN 230
Query: 543 ICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
ICYGRHHPLNWLM+ +NGAEI+FNPSAT+ LSE +W +EARNAAIAN +TCA+NR
Sbjct: 231 ICYGRHHPLNWLMYSVNGAEIIFNPSATIGELSESMWPIEARNAAIANHCFTCALNR 287
>UniRef50_A7SG03 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 359
Score = 250 bits (613), Expect = 2e-65
Identities = 119/237 (50%), Positives = 158/237 (66%)
Frame = +3
Query: 3 FEIKAYDFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQ 182
FE+ Y A EE R+PR+VR+G +Q+ I T+ PI +QR + +++ I+ AAA +
Sbjct: 51 FELAGYKIDAAAEELRQPRLVRIGAVQNKIVEPTNMPIAKQREGLHNRMKDIVKAAALSK 110
Query: 183 VNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDD 362
VN++C QE W MPFAFCTREKQPW +FAE GP+ E A +Y+MVI+SPILERD
Sbjct: 111 VNVICFQECWTMPFAFCTREKQPWTEFAESAEDGPTVRLCQEWAKRYNMVIVSPILERDH 170
Query: 363 IHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAIN 542
H + +WNTAV+I+ G+VIGK RKNHIPRVGDFNESTYY EG+ GH VF+T++
Sbjct: 171 THQEILWNTAVIISNTGEVIGKTRKNHIPRVGDFNESTYYMEGDMGHQVFQTQFDT---- 226
Query: 543 ICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
GR ++W + + G+ + L E LW++EARNAAIANSY+T INR
Sbjct: 227 ---GR---ISWFLVSLQGSHYIL----VALHLCEPLWSIEARNAAIANSYFTVPINR 273
>UniRef50_Q5L031 Cluster: Beta-alanine synthase; n=19; Bacteria|Rep:
Beta-alanine synthase - Geobacillus kaustophilus
Length = 296
Score = 184 bits (447), Expect = 3e-45
Identities = 97/223 (43%), Positives = 132/223 (59%), Gaps = 6/223 (2%)
Frame = +3
Query: 63 VRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTRE 242
V +GLIQ S + D P+ + EK K++ A I+CLQE + P+ FC +
Sbjct: 5 VTIGLIQASHNVHGDEPVEVHKEKAIEKHVKLVKEAKDRGAQIICLQEIFYGPY-FCAEQ 63
Query: 243 KQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVI 422
W + AE + GP+T E+A + +VI+ PI ER+ I T +NTA VI+ G +
Sbjct: 64 NTKWYEAAEEIPNGPTTKMFQEIAKQLGVVIVLPIYEREGIA--TYYNTAAVIDADGTYL 121
Query: 423 GKHRKNHIPRVGDFNEST-----YYFE-GNTGHPVFETKYGKVAINICYGRHHPLNWLMF 584
GK+RK HIP VG NE +YF+ GN G+ VF+T + K+ + ICY RH P +
Sbjct: 122 GKYRKQHIPHVGVGNEGCGFWEKFYFKPGNLGYSVFDTAFAKIGVYICYDRHFPEGARIL 181
Query: 585 GINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
G+ GAEIVFNPSATV+GLSE+LW +E A+AN YY AINR
Sbjct: 182 GLKGAEIVFNPSATVAGLSEYLWKLEQPAHAVANGYYVAAINR 224
>UniRef50_O61697 Cluster: Putative beta-ureidopropionase; n=1;
Manduca sexta|Rep: Putative beta-ureidopropionase -
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Length = 185
Score = 170 bits (414), Expect = 3e-41
Identities = 75/117 (64%), Positives = 92/117 (78%)
Frame = +3
Query: 3 FEIKAYDFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQ 182
FE+ AY FPA+KE+ R PRIV++G+IQHSI TD P+ +Q+ AIF+KV+KII A E
Sbjct: 51 FEVAAYAFPAKKEQTRPPRIVKVGVIQHSIGAPTDRPVNEQKKAIFDKVKKIIDVAGQEG 110
Query: 183 VNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILE 353
VNI+C QE WNMPFAFCTREKQPWC+FAE GP+T FL ELA+KY MVI+S IL+
Sbjct: 111 VNIICFQELWNMPFAFCTREKQPWCEFAESAEEGPTTRFLRELAMKYSMVIVSSILD 167
>UniRef50_Q2S196 Cluster: Hydrolase, carbon-nitrogen family; n=1;
Salinibacter ruber DSM 13855|Rep: Hydrolase,
carbon-nitrogen family - Salinibacter ruber (strain DSM
13855)
Length = 283
Score = 99.1 bits (236), Expect = 9e-20
Identities = 63/219 (28%), Positives = 114/219 (52%), Gaps = 4/219 (1%)
Frame = +3
Query: 69 LGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPF---AFCTR 239
+ L+QH+++ P + R+ ++ + + AAA +++ E PF
Sbjct: 3 IALVQHAVS-----PASPPRV---DRGVRAVQAAADAGADLVVFPELSFTPFYPRVPVAE 54
Query: 240 EKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKV 419
++ D AEPV GP+T LAE A +V++ ++ERD G+ ++T+ V++ G +
Sbjct: 55 RRRSARDLAEPV-PGPTTEALAEAAADGGVVVVFNLMERD---GERTFDTSPVLDADGTL 110
Query: 420 IGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGA 599
+G+ R HI +F+E YY G+TG PV++T G++ + +CY RH+P + A
Sbjct: 111 LGRTRMMHITAYENFHEQGYYDPGDTGAPVYDTAAGRIGVAVCYDRHYPEYLRALALQDA 170
Query: 600 EIVFNPSA-TVSGLSEHLWAVEARNAAIANSYYTCAINR 713
++V P A TV + ++ E R AA+ + ++ NR
Sbjct: 171 DLVVVPQAGTVGEWPDGMYEAELRVAALQHGFFAALANR 209
>UniRef50_Q1IQA8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=52; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Acidobacteria bacterium (strain
Ellin345)
Length = 303
Score = 96.3 bits (229), Expect = 7e-19
Identities = 66/224 (29%), Positives = 111/224 (49%), Gaps = 9/224 (4%)
Frame = +3
Query: 69 LGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQ 248
+GLIQ S P+ ++ +A K + AA + ++CL E + + FC RE
Sbjct: 8 IGLIQMSCG-----PVPEENMA---KALDRVRDAAKQGATVICLPELFQTQY-FCQREDT 58
Query: 249 PWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGK 428
+ AE + GP+T + +LA + +V+++ + ER NTA +++E G + G
Sbjct: 59 ALFELAESI-PGPATKKMGDLARELGVVVVASLFERRA--PGLYHNTAAILDEAGALKGI 115
Query: 429 HRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIV 608
+RK HIP + E Y+ G+ G FETK+G + +C+ + +P + + GA+++
Sbjct: 116 YRKMHIPDDPLYYEKYYFTPGDLGFKTFETKFGPIGTLVCWDQWYPEGARLTALQGAQVL 175
Query: 609 FNPSA--------TVSGLSEH-LWAVEARNAAIANSYYTCAINR 713
F P+A G S+H W R+ AIAN Y +NR
Sbjct: 176 FYPTAIGWHPAEKAEFGESQHDAWRTIQRSHAIANGVYVGVVNR 219
>UniRef50_Q972L1 Cluster: 281aa long hypothetical
beta-ureidopropionase; n=1; Sulfolobus tokodaii|Rep:
281aa long hypothetical beta-ureidopropionase -
Sulfolobus tokodaii
Length = 281
Score = 95.1 bits (226), Expect = 2e-18
Identities = 61/198 (30%), Positives = 96/198 (48%)
Frame = +3
Query: 120 QQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVF 299
+ + A +K + AA + ++ E + + F E + D AEP GP+
Sbjct: 16 ESKEANIQKALEYTKAAVKDGAELIVYNELFTTQY-FPATEDPKFFDLAEPE-DGPTVRV 73
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
AE + +Y + +I I E D ++TA+ I + GKV+GK+RK HIP+V + E +
Sbjct: 74 FAEFSKQYKIGMIITIFEEDKKIKGIYYDTAIFIKD-GKVLGKYRKTHIPQVPGYYEK-F 131
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAV 659
YF+ +PVF+ K+ ICY RH P + + GA+IV P T + W +
Sbjct: 132 YFKPGKEYPVFDFGGYKIGAVICYDRHFPEGVRILTLKGADIVTIP--TTTNFYPETWEL 189
Query: 660 EARNAAIANSYYTCAINR 713
E R A N+ Y +NR
Sbjct: 190 ELRAHAAFNTIYVVGVNR 207
>UniRef50_A4J4S3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Desulfotomaculum
reducens MI-1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Desulfotomaculum
reducens MI-1
Length = 273
Score = 94.3 bits (224), Expect = 3e-18
Identities = 62/185 (33%), Positives = 93/185 (50%)
Frame = +3
Query: 159 ISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVII 338
I AAA I+CL E + E + W + EPV GP+T ++LA + + II
Sbjct: 30 IRQAAAMGAQIICLPELCTTGYRPDLLEDKLW-ELTEPV-PGPTTDVFSQLAKELGIYII 87
Query: 339 SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFET 518
P+ E+ + G I N+AV I++ G+V G RK H + YYF +PVF+T
Sbjct: 88 LPMNEKGAVPG-MIHNSAVFIDKDGEVQGVFRKAHA-----YATERYYFTDGNHYPVFQT 141
Query: 519 KYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYT 698
++GKV + ICY P + + GAE++F PSA E +W + A+ N +
Sbjct: 142 EFGKVGVMICYDMGFPEVARILTLKGAEVIFAPSAWRQE-DEDIWDINIAARALENRLFV 200
Query: 699 CAINR 713
A+NR
Sbjct: 201 AAVNR 205
>UniRef50_Q972X1 Cluster: 264aa long hypothetical
beta-ureidopropionase; n=1; Sulfolobus tokodaii|Rep:
264aa long hypothetical beta-ureidopropionase -
Sulfolobus tokodaii
Length = 264
Score = 93.5 bits (222), Expect = 5e-18
Identities = 59/191 (30%), Positives = 102/191 (53%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
E+ ++++ A + I+ L E N + F + + +AE G + E++ +
Sbjct: 19 ERQVELVNKAIDNKAKIIALDELSNTIY-FPFEQNPKYFSWAETE-RGETLQRFKEISKE 76
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
++ +I PI ERD + +NTA +++ G++IGK+RK H+P+ FNE Y+ G+ G
Sbjct: 77 REVSLIVPIFERDS---NFFYNTAFILDN-GEIIGKYRKTHLPQEEFFNEYYYFKVGDLG 132
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAI 680
P+F+ K K + IC+ RH P + I GA ++F PS V+ E +W +E + A+
Sbjct: 133 FPIFDLKGVKTGVVICHDRHFPEPVRVEVIKGAWLIFIPS--VAAFKE-IWELELKAHAV 189
Query: 681 ANSYYTCAINR 713
N+ Y INR
Sbjct: 190 FNTVYIAGINR 200
>UniRef50_A7I5W9 Cluster: Porphyromonas-type peptidyl-arginine
deiminase; n=1; Candidatus Methanoregula boonei 6A8|Rep:
Porphyromonas-type peptidyl-arginine deiminase -
Methanoregula boonei (strain 6A8)
Length = 640
Score = 93.5 bits (222), Expect = 5e-18
Identities = 72/223 (32%), Positives = 107/223 (47%), Gaps = 8/223 (3%)
Frame = +3
Query: 69 LGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQ 248
+ LIQ I D + + R E+V+K AA +CL E + + F +
Sbjct: 8 IALIQMEIGPDPDRNLNEAR----ERVEK----AAQNGAQFICLPELFRTRY-FPQQIGT 58
Query: 249 PWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGK 428
P AE + G ST +A +Y VII P+ ER + + N AVVI+ G +
Sbjct: 59 PVQSLAETI-PGESTDVFTRIAKEYKAVIIVPVFERSPL--GHLENAAVVIDADGSLHAP 115
Query: 429 HRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIV 608
+ K HIP+ F E Y++ GN + V T+YGK+A+ ICY + P + GAEI+
Sbjct: 116 YYKVHIPQDPKFFEKGYFYPGNH-YAVHATRYGKIAVLICYDQWFPEAARCVSLEGAEII 174
Query: 609 FNPSATVSGLSE--------HLWAVEARNAAIANSYYTCAINR 713
F P+A + +E W + R+ AIANS + A+NR
Sbjct: 175 FYPTAIGNPCTEQPSEGDWQEAWEIIQRSHAIANSVHIAAVNR 217
>UniRef50_Q97RA3 Cluster: Carbon-nitrogen hydrolase family protein;
n=24; Bacteria|Rep: Carbon-nitrogen hydrolase family
protein - Streptococcus pneumoniae
Length = 291
Score = 92.7 bits (220), Expect = 8e-18
Identities = 47/161 (29%), Positives = 85/161 (52%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
+ ++++ AA + I+ L E + P+ FC + + +A+ V + +A +
Sbjct: 22 QTAERLVRQAAEQGAQIILLPELFEHPY-FCQERQYDYYQYAQSVAENTAIQHFKVIAKE 80
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
+V+ E+D G+ ++N+ VI+ G+V+G +RK HIP + E Y+ GNTG
Sbjct: 81 LQVVLPISFYEKD---GNVLYNSIAVIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTG 137
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
V+ T+Y K+ I IC+ + P +NGAE++F P+A
Sbjct: 138 FKVWNTRYAKIGIGICWDQWFPETARCLALNGAELLFYPTA 178
>UniRef50_A6DKQ0 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Lentisphaera araneosa HTCC2155|Rep: Carbon-nitrogen
hydrolase family protein - Lentisphaera araneosa
HTCC2155
Length = 286
Score = 91.1 bits (216), Expect = 2e-17
Identities = 61/196 (31%), Positives = 99/196 (50%), Gaps = 9/196 (4%)
Frame = +3
Query: 153 KIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMV 332
K+I+ AA NI+C QE + + FC + +A+ + F + A + +V
Sbjct: 24 KLIADAAKSGANIICTQELFLSNY-FCREQNTEHFQYAQKIDQELLADF-QQCAKNHGVV 81
Query: 333 IISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVF 512
+ E + ++G +NT+V+I+ G +GK+RK HIP+ F E Y+ GN G PVF
Sbjct: 82 LALSFFE-EALNG-VYYNTSVIIDADGTYLGKYRKLHIPQDPYFEEKFYFTPGNLGVPVF 139
Query: 513 ETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA--------TVSGLSE-HLWAVEA 665
ET++GK+++ IC+ + P + + GAEI+ P+A G + H W
Sbjct: 140 ETQFGKISLIICWDQWFPETARLACLAGAEIILVPTAIGWLPDEKEEHGAQQAHSWTQVQ 199
Query: 666 RNAAIANSYYTCAINR 713
A+AN Y A+NR
Sbjct: 200 LGHAVANGCYYAAVNR 215
>UniRef50_A4B9A7 Cluster: Probable hydratase; n=2; Bacteria|Rep:
Probable hydratase - Reinekea sp. MED297
Length = 289
Score = 89.4 bits (212), Expect = 8e-17
Identities = 46/158 (29%), Positives = 85/158 (53%)
Frame = +3
Query: 150 QKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDM 329
++++ AAA ++ LQE + P+ FC +K+ + FA + P+ A +A + +
Sbjct: 25 ERLVREAAASGAQVILLQELFERPY-FCQHQKEEFRRFATAIDDNPAIAHFAPIARELGV 83
Query: 330 VIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPV 509
V+ E+ G +N+ VV++ G+ +G +RK HIP + E Y+ G+TG V
Sbjct: 84 VLPISFFEQC---GPVAYNSVVVLDADGENLGLYRKTHIPDGPGYCEKFYFTPGDTGFQV 140
Query: 510 FETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
F T++G++ + IC+ + P + GAE++F P+A
Sbjct: 141 FSTRFGRIGVGICWDQWFPETARAMTLMGAELLFYPTA 178
>UniRef50_Q1GTC5 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=9; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Sphingopyxis alaskensis
(Sphingomonas alaskensis)
Length = 300
Score = 87.4 bits (207), Expect = 3e-16
Identities = 56/195 (28%), Positives = 92/195 (47%), Gaps = 6/195 (3%)
Frame = +3
Query: 147 VQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYD 326
V ++ AAAA I+ E + P+ FC E++ A P PS V + LA K
Sbjct: 42 VTALVEAAAARGAQIILPPELFEGPY-FCQVEEEELFATARPTAEHPSVVAMQALAAKCK 100
Query: 327 MVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP 506
+ I + ERD H +NT +I G ++G +RK+HIP + E Y+ GNTG
Sbjct: 101 VAIPTSFFERDGHH---YYNTLAMIGPDGGIMGTYRKSHIPDGPGYEEKYYFRPGNTGFK 157
Query: 507 VFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGL------SEHLWAVEAR 668
++E ++ + +C+ + +P + GAE++F P+A S + +W +
Sbjct: 158 IWEVFDTRIGVGVCWDQWYPECARAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMQ 217
Query: 669 NAAIANSYYTCAINR 713
A++N A NR
Sbjct: 218 GHAVSNCMPVIAANR 232
>UniRef50_Q9ABL5 Cluster: Hydrolase, carbon-nitrogen family; n=13;
Bacteria|Rep: Hydrolase, carbon-nitrogen family -
Caulobacter crescentus (Caulobacter vibrioides)
Length = 292
Score = 85.4 bits (202), Expect = 1e-15
Identities = 48/164 (29%), Positives = 87/164 (53%)
Frame = +3
Query: 132 AIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAEL 311
A +K + I AA++ ++ E + P+ FC +++ W A P P +A L
Sbjct: 20 ANIKKTEGFIREAASKGAQVILPSELFQGPY-FCVAQEERWFAQAHPWREHPVVKAIAPL 78
Query: 312 AVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEG 491
A + +VI I ER+ H +N+ V+ + G ++G +RK+HIP + E Y+ G
Sbjct: 79 AGELGVVIPISIFEREGPH---YFNSLVMADADGSLMGVYRKSHIPDGPGYMEKYYFRPG 135
Query: 492 NTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+TG V++T++G++ + IC+ + +P + GAE +F P+A
Sbjct: 136 DTGFKVWDTRFGRIGVGICWDQWYPECARAMALMGAEALFYPTA 179
>UniRef50_Q89413 Cluster: A78R protein; n=6; Chlorovirus|Rep: A78R
protein - Paramecium bursaria Chlorella virus 1 (PBCV-1)
Length = 298
Score = 83.4 bits (197), Expect = 5e-15
Identities = 44/161 (27%), Positives = 81/161 (50%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
E+ + ++ AAA ++ LQE + + FC + + FA+P ++LA +
Sbjct: 23 ERAEMLVRNAAANGAQVIVLQELFATKY-FCQTQSPQYFKFADPADDSVIVEIFSKLAKE 81
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
+VI P E+D G+ +N+ V + G ++G +RK HIP+ + E Y+ +
Sbjct: 82 LGVVIPIPFFEKD---GNNYYNSVAVADADGSIVGVYRKTHIPQSKCYEEKFYFTPSSNP 138
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+ VFETK+GK+ + IC+ + + GA+ + P+A
Sbjct: 139 YEVFETKFGKMGVLICWDQWFSEAAKCLALEGADFIVYPTA 179
>UniRef50_A6CCK5 Cluster: Putative uncharacterized protein; n=1;
Planctomyces maris DSM 8797|Rep: Putative
uncharacterized protein - Planctomyces maris DSM 8797
Length = 450
Score = 82.2 bits (194), Expect = 1e-14
Identities = 71/228 (31%), Positives = 111/228 (48%), Gaps = 2/228 (0%)
Frame = +3
Query: 21 DFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCL 200
D ++ + +PRIVRL I H + A K+I AA ++ +++ L
Sbjct: 183 DINLKQTKDLQPRIVRLATIHHRPQAGKKPSDKPAQFA------KLIEQAAEQKADLVVL 236
Query: 201 QEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTI 380
E+ + + + + AEP+ GPST + ELA K+D+ I+ + ER +
Sbjct: 237 PESITVYGTGLS-----YAETAEPI-PGPSTQYFGELAKKHDLYIVVGLYERA---AHLV 287
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRH 560
+N AV+I GKV+GK+RK +PR G+ GN +PVFET++GKV + +CY
Sbjct: 288 YNVAVLIGPDGKVVGKYRKVTLPR-GEIEGGV--TPGNE-YPVFETRFGKVGMMVCYDGF 343
Query: 561 HPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEA--RNAAIANSYYT 698
P NGAE++ P V G + L A A + + +S YT
Sbjct: 344 FPEVARELSKNGAEVIAWP---VWGCNPLLGAARACENHVYVISSTYT 388
>UniRef50_Q8VYF5 Cluster: N-carbamoylputrescine amidase; n=60;
cellular organisms|Rep: N-carbamoylputrescine amidase -
Arabidopsis thaliana (Mouse-ear cress)
Length = 326
Score = 80.6 bits (190), Expect = 4e-14
Identities = 47/157 (29%), Positives = 85/157 (54%), Gaps = 1/157 (0%)
Frame = +3
Query: 156 IISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVI 335
++ A A+ NI+ +QE + + FC +++ + A+P P+ + +LA + +VI
Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118
Query: 336 -ISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVF 512
+S E + H +N+ +I+ G +G +RK+HIP + E Y+ G+TG VF
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 174
Query: 513 ETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+TK+ K+ + IC+ + P + GAEI+F P+A
Sbjct: 175 QTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTA 211
>UniRef50_Q9UYV8 Cluster: Beta ureidopropionase; n=4;
Thermococcaceae|Rep: Beta ureidopropionase - Pyrococcus
abyssi
Length = 262
Score = 78.2 bits (184), Expect = 2e-13
Identities = 53/193 (27%), Positives = 98/193 (50%), Gaps = 1/193 (0%)
Frame = +3
Query: 138 FEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAV 317
+ K +K+I A+ + ++ L E ++ + F TRE+ + A+ + G +T FL ++A
Sbjct: 20 YSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEV--FEIAQKIPEGETTTFLMDVAR 77
Query: 318 KYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFE-GN 494
+ I++ E+D GD ++N+AVV+ G IGK+RK H+ F ++FE G+
Sbjct: 78 DTGVYIVAGTAEKD---GDVLYNSAVVVGPRG-FIGKYRKIHL-----FYREKFFFEPGD 128
Query: 495 TGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNA 674
G VF+ + KV + IC+ P + + GA+++ +P+ V + +
Sbjct: 129 LGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVIAHPANLVMPYAPRAMPIR---- 184
Query: 675 AIANSYYTCAINR 713
A+ N YT +R
Sbjct: 185 ALENKVYTVTADR 197
>UniRef50_A7I2D9 Cluster: Hydrolase, carbon-nitrogen family; n=1;
Campylobacter hominis ATCC BAA-381|Rep: Hydrolase,
carbon-nitrogen family - Campylobacter hominis (strain
ATCC BAA-381 / LMG 19568 / NCTC 13146 /CH001A)
Length = 336
Score = 77.8 bits (183), Expect = 2e-13
Identities = 55/200 (27%), Positives = 92/200 (46%), Gaps = 9/200 (4%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
+K ++I A + ++ LQE + FC E+ +FA S F E A K
Sbjct: 22 QKSVEMIEKVAKDGAKLVILQELHEWAY-FCQSERVE--NFALAENFNESLKFWGETAKK 78
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
+ +V+++ + E+ NTA+V G++ GK+RK HIP +F E Y+ G+ G
Sbjct: 79 FGIVLVTSLFEKRA--PGLFHNTAIVFENNGEIAGKYRKMHIPDDPNFYEKFYFTPGDLG 136
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSAT--VSGLSE-------HLW 653
T G++ + +C+ + +P + + GAEI+ P+A G E W
Sbjct: 137 FEPINTSVGRLGVLVCWDQWYPEAARLMALKGAEILIYPTAIGWFDGDDEAEKSRQLEAW 196
Query: 654 AVEARNAAIANSYYTCAINR 713
R A+AN+ A+NR
Sbjct: 197 VAVQRGHAVANALPVIAVNR 216
>UniRef50_A0QWL8 Cluster: Carbon-nitrogen hydrolase family protein;
n=6; Bacteria|Rep: Carbon-nitrogen hydrolase family
protein - Mycobacterium smegmatis (strain ATCC 700084 /
mc(2)155)
Length = 299
Score = 75.8 bits (178), Expect = 1e-12
Identities = 52/189 (27%), Positives = 99/189 (52%), Gaps = 1/189 (0%)
Frame = +3
Query: 66 RLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREK 245
R+ ++Q + + +N + A++E++Q+ ++ A N++ L E + F +RE+
Sbjct: 13 RVAVVQFNPQVGVEN-LKANSEAVYERLQQAVAGGA----NLIVLPELATTGYTFESREE 67
Query: 246 QPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIG 425
AEPV +G + AE A +D+ I+ + E D + +++TAV++ G IG
Sbjct: 68 A--YAHAEPVPSGATVTGWAEFAAAHDVYIVGCLPELDGVE---LFDTAVLVGPEG-YIG 121
Query: 426 KHRKNHIPRVGDFNESTYYFE-GNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAE 602
K+RK H+ +NE +F G+ G+PVF T+ G++ + +C+ P + GA+
Sbjct: 122 KYRKTHL-----WNEEKLFFSPGDLGYPVFHTRIGRIGLLVCWDIWFPETARIVAQQGAD 176
Query: 603 IVFNPSATV 629
I+ P+ V
Sbjct: 177 IICIPTGWV 185
>UniRef50_A6QC56 Cluster: Hydrolase; n=2; Bacteria|Rep: Hydrolase -
Sulfurovum sp. (strain NBC37-1)
Length = 290
Score = 74.1 bits (174), Expect = 3e-12
Identities = 55/194 (28%), Positives = 89/194 (45%), Gaps = 9/194 (4%)
Frame = +3
Query: 159 ISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVII 338
I AA+ ++ LQE + FC E + D+A S F +A K+ +V++
Sbjct: 25 IEEAASNSTELIVLQELHQNEY-FCQSEDTAFFDYAADFDADVS--FWGAVAKKHGIVLV 81
Query: 339 SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFET 518
+ + E+ NTAVV + G + GK+RK HIP F E Y+ G+ G ET
Sbjct: 82 TSLFEKRA--PGLYHNTAVVFEKDGNIAGKYRKMHIPDDPGFYEKFYFTPGDLGFEPIET 139
Query: 519 KYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA-------TVSGLSEHL--WAVEARN 671
GK+ + +C+ + +P + + GA+++ P+A T + L W R+
Sbjct: 140 SVGKLGVLVCWDQWYPEAARIMALKGAQLLIYPTAIGWFDEDTDKEKARQLDSWITIQRS 199
Query: 672 AAIANSYYTCAINR 713
AIAN + NR
Sbjct: 200 HAIANGIPVLSCNR 213
>UniRef50_Q606Z9 Cluster: Hydrolase, carbon-nitrogen family; n=38;
Bacteria|Rep: Hydrolase, carbon-nitrogen family -
Methylococcus capsulatus
Length = 295
Score = 73.7 bits (173), Expect = 4e-12
Identities = 54/194 (27%), Positives = 92/194 (47%), Gaps = 9/194 (4%)
Frame = +3
Query: 159 ISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVII 338
I + A+ +++ L E P+ FC E D AE + GP+T L +A + +V++
Sbjct: 29 IRRSKAKGADLVMLPELHLGPY-FCQTEDCSCFDGAETI-PGPTTAELGSVARELGVVVV 86
Query: 339 SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFET 518
+ + ER NTAVV++ G + GK+RK HIP + E Y+ G+ G +T
Sbjct: 87 ASLFERRA--PGLYHNTAVVLDSDGSLAGKYRKMHIPDDPGYYEKFYFTPGDLGFRPIDT 144
Query: 519 KYGKVAINICYGRHHPLNWLMFGINGAEIV-------FNPSATVSGLSEHL--WAVEARN 671
G++ + +C+ + +P + + GA+++ +NP+ S L W R
Sbjct: 145 SVGRLGVLVCWDQWYPEAARLMALAGADLLLYPTAIGWNPADDEVERSRQLEAWITVQRG 204
Query: 672 AAIANSYYTCAINR 713
A+AN A NR
Sbjct: 205 HAVANGLTVAACNR 218
>UniRef50_A6TPX2 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Alkaliphilus
metalliredigens QYMF|Rep: Nitrilase/cyanide hydratase
and apolipoprotein N-acyltransferase - Alkaliphilus
metalliredigens QYMF
Length = 269
Score = 73.7 bits (173), Expect = 4e-12
Identities = 55/200 (27%), Positives = 93/200 (46%)
Frame = +3
Query: 111 PITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPS 290
PI A ++ Q I AAA++V+++ L E W + K+ + AE G +
Sbjct: 11 PIMNDVEANLKRGQHFIQQAAAQEVDLIVLPELWTTGYYL---SKESFKQLAEHK-DGRT 66
Query: 291 TVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNE 470
+ + A++ + II P +E + ++ A VI+ G++ G K+ + E
Sbjct: 67 VTLMQDQALRSNASIICPFVEITE--DKKLYIAAAVIDHRGELRGTVHKSLLWG----RE 120
Query: 471 STYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHL 650
+ EGN +PVF+TK GKV I ICY P + + G E++ PS S + H
Sbjct: 121 QQIFEEGNIEYPVFDTKIGKVGILICYEMEFPETSRLLALQGVEMIVCPSVW-SLSASHR 179
Query: 651 WAVEARNAAIANSYYTCAIN 710
W ++ A+ N+ Y +N
Sbjct: 180 WDIQLPARALDNTVYVFGVN 199
>UniRef50_Q2AH52 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Halothermothrix
orenii H 168|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Halothermothrix
orenii H 168
Length = 273
Score = 73.3 bits (172), Expect = 5e-12
Identities = 46/145 (31%), Positives = 70/145 (48%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRV 455
+ G +T +E A Y II ++ERD G+ ++NT VI++ G GK+RK H+
Sbjct: 65 IPGRTTEIFSEYARMYKTAIIGNMVERDKNVGEILYNTTFVIDKKGDYTGKYRKVHV--- 121
Query: 456 GDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSG 635
+ YF+ T PVF K+ + CY + + GA+I+F PSA G
Sbjct: 122 --YPAEFTYFKRGTEFPVFNVNGVKIGLATCYDHGFGEMFRILARKGAQIIFIPSAIPKG 179
Query: 636 LSEHLWAVEARNAAIANSYYTCAIN 710
E+L + R A N +T A+N
Sbjct: 180 Y-EYLLKLRTRARAQDNQLFTVAVN 203
>UniRef50_Q0AX54 Cluster: N-carbamoyl-D-amino acid amidohydrolase;
n=1; Syntrophomonas wolfei subsp. wolfei str.
Goettingen|Rep: N-carbamoyl-D-amino acid amidohydrolase
- Syntrophomonas wolfei subsp. wolfei (strain
Goettingen)
Length = 283
Score = 72.5 bits (170), Expect = 9e-12
Identities = 60/194 (30%), Positives = 93/194 (47%), Gaps = 6/194 (3%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
+K ++I+AAA E ++ L E +N P+ + + + +AEP GPST FLA A K
Sbjct: 23 KKAGEMIAAAAGEGAEMVVLPEVFNSPY-----QAELFPRYAEP-FPGPSTDFLAAAACK 76
Query: 321 YDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYY 482
+ + I+ I+ERD I+N++ V +E G++IG+HRK H IP F ES
Sbjct: 77 HGLCIVGGSIIERDS--QGKIYNSSFVFDERGELIGRHRKAHLFDIDIPGRISFRESDTL 134
Query: 483 FEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVE 662
G + K A+ ICY P + GAE++ P+A + W +
Sbjct: 135 NAGE-NITIVHYKSRLFALMICYDCRFPELARAAALEGAELLVIPAAFNTTTGPAHWKLL 193
Query: 663 ARNAAIANSYYTCA 704
R A+ N + A
Sbjct: 194 MRCRAVDNQLFVVA 207
>UniRef50_Q0LQX0 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Herpetosiphon
aurantiacus ATCC 23779|Rep: Nitrilase/cyanide hydratase
and apolipoprotein N-acyltransferase - Herpetosiphon
aurantiacus ATCC 23779
Length = 259
Score = 72.1 bits (169), Expect = 1e-11
Identities = 56/212 (26%), Positives = 97/212 (45%)
Frame = +3
Query: 78 IQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWC 257
+Q ++A++ + + R A V+++ + A +L L E W + +
Sbjct: 1 MQLTVALAQIDLVLGDREANLATVRQLAARAEMAGAALLVLPELWGTGYLL-----EQAH 55
Query: 258 DFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRK 437
+ ++P+ G +A LA ++ + I+ +LERD G+ ++NTA + + GK + +RK
Sbjct: 56 ELSDPLGKGLFEE-VAVLAARHHLAIVGSLLERD---GEQVYNTATLYDAQGKRLHSYRK 111
Query: 438 NHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNP 617
H+ +G E Y G VFET +G A ICY P + + + GA ++ P
Sbjct: 112 THL--IGLMQEDRYLAAGQQAE-VFETAWGTSACAICYDLRFPELFRRYALAGAGVIIIP 168
Query: 618 SATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
+ + EH W R AI N A NR
Sbjct: 169 AEWPTARIEH-WRTLLRARAIENQAVVIACNR 199
>UniRef50_A7GE66 Cluster: Hydrolase, carbon-nitrogen family; n=13;
cellular organisms|Rep: Hydrolase, carbon-nitrogen
family - Clostridium botulinum (strain Langeland / NCTC
10281 / Type F)
Length = 278
Score = 70.9 bits (166), Expect = 3e-11
Identities = 50/203 (24%), Positives = 94/203 (46%), Gaps = 5/203 (2%)
Frame = +3
Query: 114 ITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPST 293
+ +++ +K ++++ A E NI L E +N P+ + +P+ + G +
Sbjct: 13 VQKEKKKNIKKAIEMLTKAKKENCNIAVLPEMFNCPYE--NKCFKPYGEIINEENGGETV 70
Query: 294 VFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVG----- 458
+ + A ++ I++ + +I GD I+NT++V + G +I KHRK H+ +
Sbjct: 71 KAIKKAAKDLELYIVAGSIP--EIEGDKIYNTSMVFDNKGVLIAKHRKVHLFDIDVKGGV 128
Query: 459 DFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGL 638
F ES GN +F T +GK+ + ICY P + + GA+I+F P+A
Sbjct: 129 TFKESDTLTAGNK-ITLFNTPWGKLGVMICYDIRFPELSRIMAVKGAKIIFTPAAFNMTT 187
Query: 639 SEHLWAVEARNAAIANSYYTCAI 707
W ++ A+ N Y +
Sbjct: 188 GPAHWDTLFKSRALDNQVYMVGV 210
>UniRef50_Q6AHZ8 Cluster: Putative uncharacterized protein
DKFZp779O1248; n=1; Homo sapiens|Rep: Putative
uncharacterized protein DKFZp779O1248 - Homo sapiens
(Human)
Length = 186
Score = 70.1 bits (164), Expect = 5e-11
Identities = 36/97 (37%), Positives = 58/97 (59%), Gaps = 1/97 (1%)
Frame = +3
Query: 3 FEIKAYDFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQ 182
FE++ Y F A +E+ R+PRIV +GL+Q+ I + + P+ +Q A+ +++ I+ AA
Sbjct: 52 FELQGYAFEAAEEQLRRPRIVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCG 111
Query: 183 VNILCLQEAWNM-PFAFCTREKQPWCDFAEPVLTGPS 290
VNI+C QEAW + P +E +P C +A PS
Sbjct: 112 VNIICFQEAWILRPH---HQEPRPPCCYAPSCCLIPS 145
>UniRef50_Q7M8G2 Cluster: HYDROLASE-Predicted amidohydrolase; n=5;
Bacteria|Rep: HYDROLASE-Predicted amidohydrolase -
Wolinella succinogenes
Length = 290
Score = 69.3 bits (162), Expect = 9e-11
Identities = 51/205 (24%), Positives = 89/205 (43%), Gaps = 9/205 (4%)
Frame = +3
Query: 126 RLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLA 305
R A ++ +++I A+ ++ +QE + FC E+ + D+A +
Sbjct: 14 REATIQRSRELILEASKGGAELVVMQELHTSEY-FCQSEETRFFDYAS--FYEEDVRIFS 70
Query: 306 ELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYF 485
+A + +V++ ER NTAVV + G + G++RK HIP F E Y+
Sbjct: 71 SIAKEGGVVLVGSFFERRS--AGIYHNTAVVFEKDGSIAGRYRKMHIPDDPGFYEKFYFT 128
Query: 486 EGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA----TVSGLSE--- 644
G+ G GK+ + +C+ + +P + + GA+I+ P+A L E
Sbjct: 129 PGDLGFEPISCSLGKLGVLVCWDQWYPEAARLMALKGADILLYPTAIGWFDADDLDEKER 188
Query: 645 --HLWAVEARNAAIANSYYTCAINR 713
W R A+AN A+NR
Sbjct: 189 QKEAWIAIQRGHAVANGLPVVAVNR 213
>UniRef50_O30121 Cluster: Putative uncharacterized protein; n=1;
Archaeoglobus fulgidus|Rep: Putative uncharacterized
protein - Archaeoglobus fulgidus
Length = 257
Score = 69.3 bits (162), Expect = 9e-11
Identities = 46/137 (33%), Positives = 75/137 (54%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
L +++ + D++II+ + ER+ GD ++N+AV+I++ GK+IGK+RK H+ + NE Y
Sbjct: 70 LLKISEQKDIMIITGVAERE---GDDLYNSAVIIHK-GKIIGKYRKTHLFPLT--NEKKY 123
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAV 659
+ G+ VFET GK+ + ICY P GAEI+ P+ +H W V
Sbjct: 124 FKAGDKLE-VFETHLGKIGLLICYEVRFPELSRKLVKMGAEIIVIPAEFPKERIDH-WRV 181
Query: 660 EARNAAIANSYYTCAIN 710
+ AI N + +N
Sbjct: 182 LLQARAIENQVFVAGVN 198
>UniRef50_Q183H2 Cluster: Putative carbon-nitrogen hydrolase; n=2;
Clostridium difficile|Rep: Putative carbon-nitrogen
hydrolase - Clostridium difficile (strain 630)
Length = 268
Score = 68.5 bits (160), Expect = 2e-10
Identities = 40/139 (28%), Positives = 73/139 (52%), Gaps = 2/139 (1%)
Frame = +3
Query: 300 LAELAVKYDMVIISPI--LERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNES 473
++E A + ++ +ISP LE+ H ++N+AV+ + GK++G++ KNH+ ++
Sbjct: 76 MSEAAKRNNVYLISPYGTLEKGSTH---VYNSAVIFDRKGKIMGEYCKNHL-----WSLE 127
Query: 474 TYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLW 653
YF+G V++ +G+ + ICY P + G+EI+F PSA E +W
Sbjct: 128 AVYFKGGEKVEVYDADFGRFGVMICYDAGFPEVSRELTLKGSEIIFIPSAW-RIQDEDMW 186
Query: 654 AVEARNAAIANSYYTCAIN 710
+ A+ N+ YT +N
Sbjct: 187 DLNVSQRALENTVYTVGVN 205
>UniRef50_A6DDT2 Cluster: HYDROLASE-Predicted amidohydrolase; n=1;
Caminibacter mediatlanticus TB-2|Rep:
HYDROLASE-Predicted amidohydrolase - Caminibacter
mediatlanticus TB-2
Length = 299
Score = 68.1 bits (159), Expect = 2e-10
Identities = 41/145 (28%), Positives = 76/145 (52%)
Frame = +3
Query: 189 ILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIH 368
++ LQE + FC E + D+AE F ++ ++V+++ + E+ +
Sbjct: 32 LVILQELHQNEY-FCKCENTKYFDYAESF--NEDVEFWRRVSEDKNIVLVTSLFEK--VM 86
Query: 369 GDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINIC 548
+NTAVV ++ GK+ GK+RK HIP F E Y+ G+ P+ +T G++ + +C
Sbjct: 87 DGIYYNTAVVFDK-GKIAGKYRKTHIPDDPGFYEKFYFIPGDEIEPI-DTSIGRLGVLVC 144
Query: 549 YGRHHPLNWLMFGINGAEIVFNPSA 623
+ + +P + + GAEI+ P+A
Sbjct: 145 WDQWYPEPARIMALKGAEILIYPTA 169
>UniRef50_A6BCC3 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Vibrio parahaemolyticus AQ3810|Rep: Carbon-nitrogen
hydrolase family protein - Vibrio parahaemolyticus
AQ3810
Length = 167
Score = 67.7 bits (158), Expect = 3e-10
Identities = 37/122 (30%), Positives = 66/122 (54%)
Frame = +3
Query: 201 QEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTI 380
QE + P+ FC +++ + + AE ++ LA + +VI E+ G+T
Sbjct: 40 QELFAAPY-FCKKQEAKYFELAEETANSHLIQEMSALAKELGVVIPVSYFEKA---GNTF 95
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRH 560
+N+ V+I+ G V+ +RK+HIP ++E Y+ G+TG V++TK+GK IC+ +
Sbjct: 96 FNSLVMIDADGTVLDNYRKSHIPDGPGYSEKYYFSPGDTGFKVWQTKFGKFGAGICWDQW 155
Query: 561 HP 566
P
Sbjct: 156 FP 157
>UniRef50_A4M5M1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Petrotoga mobilis
SJ95|Rep: Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Petrotoga mobilis SJ95
Length = 276
Score = 66.1 bits (154), Expect = 8e-10
Identities = 41/151 (27%), Positives = 76/151 (50%)
Frame = +3
Query: 258 DFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRK 437
+ AE + G +T + +A KY++ I++ ILE+D + ++T+++I+E GK++GK+RK
Sbjct: 59 NLAEIIPDGETTQEVVRIAKKYNISIVANILEKDPLIIGKYYDTSILIDESGKLLGKYRK 118
Query: 438 NHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNP 617
+ F + + T + + K K+ ++ICY P + + + GA+I+
Sbjct: 119 IFV-----FPKEKFRLSEGTSIEIIDWKGIKIGLSICYDHAFPELYRIMALRGAQILIIT 173
Query: 618 SATVSGLSEHLWAVEARNAAIANSYYTCAIN 710
SA G E L V A N + +N
Sbjct: 174 SAVPKGF-EKLVEVRTSARAQDNQLFAIGVN 203
>UniRef50_A5C5V4 Cluster: Putative uncharacterized protein; n=1;
Vitis vinifera|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 317
Score = 66.1 bits (154), Expect = 8e-10
Identities = 39/145 (26%), Positives = 77/145 (53%), Gaps = 1/145 (0%)
Frame = +3
Query: 150 QKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDM 329
++++ A + NI+ +QE + + FC +++ + A+P P+ + + +LA + +
Sbjct: 28 ERLVRDAHRKGANIILIQELFE-GYYFCQAQREDFFQRAKPYKGHPTILRMQKLAKELGV 86
Query: 330 VI-ISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP 506
VI +S E ++ H +N+ +++ G +G +RK+HIP + E Y+ G+TG
Sbjct: 87 VIPVSFFEEANNAH----YNSIAIVDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFK 142
Query: 507 VFETKYGKVAINICYGRHHPLNWLM 581
VFETK+ K+ + + N LM
Sbjct: 143 VFETKFAKIGVGLIVILFRQTNRLM 167
>UniRef50_O59829 Cluster: Nitrilase; n=2; cellular organisms|Rep:
Nitrilase - Schizosaccharomyces pombe (Fission yeast)
Length = 272
Score = 66.1 bits (154), Expect = 8e-10
Identities = 42/150 (28%), Positives = 74/150 (49%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
AE GPS ++ LA KY + II E+++ + I+N+ + I E G + G +RK H
Sbjct: 61 AEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEKQSNIIYNSCIYITENGNLGGVYRKVH 120
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+ F+ +F+ + P+FET +GK+ + IC+ P + +NGA+++ +
Sbjct: 121 L-----FDTERKHFKKGSDFPIFETSFGKLGVMICWDTAFPEVARIHALNGADLLVVATN 175
Query: 624 TVSGLSEHLWAVEARNAAIANSYYTCAINR 713
+ S+ W + + A N A NR
Sbjct: 176 WENPYSDD-WDLVTKARAFENCIPLVAANR 204
>UniRef50_A4J6K3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Desulfotomaculum
reducens MI-1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Desulfotomaculum
reducens MI-1
Length = 277
Score = 64.5 bits (150), Expect = 2e-09
Identities = 57/190 (30%), Positives = 87/190 (45%), Gaps = 2/190 (1%)
Frame = +3
Query: 147 VQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYD 326
++K I+ AAA+Q I+C E M +RE DF + G + +FL +LA
Sbjct: 25 LEKFINEAAAQQAEIICFPE---MCIQGYSREIP---DFLLQSIDGEAILFLKKLAQNKG 78
Query: 327 MVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP 506
+ II+ + E+ + T VVI G+ I +RK H+ +E YY GN
Sbjct: 79 ITIIAGMAEK--CLNKRPFITQVVIRP-GQNIDYYRKTHLGN----SEQPYYQAGNE-IK 130
Query: 507 VFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNP--SATVSGLSEHLWAVEARNAAI 680
F T+ + I IC+ H P + + GAE++F P S T+ G + +W A
Sbjct: 131 TFSTEKTTIGIQICWDTHFPEMTTILSLRGAEVIFAPHASPTIVGDRKAIWLKYLAARAY 190
Query: 681 ANSYYTCAIN 710
NS + A N
Sbjct: 191 DNSVFLAACN 200
>UniRef50_Q8W0T9 Cluster: Putative uncharacterized protein
SB35P03.20; n=1; Sorghum bicolor|Rep: Putative
uncharacterized protein SB35P03.20 - Sorghum bicolor
(Sorghum) (Sorghum vulgare)
Length = 580
Score = 64.5 bits (150), Expect = 2e-09
Identities = 46/183 (25%), Positives = 83/183 (45%), Gaps = 6/183 (3%)
Frame = +3
Query: 162 SAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTG--PSTVFLAELAVKYDMVI 335
+AA + Q+ +E W+ C+ + +AE + G PS L+E+A + I
Sbjct: 372 AAAPSSQIKANMQKEIWS-----CSYAMETLASYAEDIDGGESPSISMLSEVAAAKKITI 426
Query: 336 ISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRV---GDFN-ESTYYFEGNTGH 503
+ + ++NT VI GK++ KHRK H+ + GD + + F G
Sbjct: 427 VGGSIPEKA--SGKMFNTCCVIGPDGKILAKHRKLHLFEIDIPGDITLKESDTFTGGQET 484
Query: 504 PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIA 683
+ +T G++ I IC+ P +++ GA ++ PSA E LW + ++ A+
Sbjct: 485 TIVDTDVGRIGIGICHDIRFPELAMLYRSKGAHLICYPSAFNMSTGELLWDLMQKSRAVD 544
Query: 684 NSY 692
N +
Sbjct: 545 NQF 547
>UniRef50_Q8RUF8 Cluster: AT5g12040/F14F18_210; n=9;
Magnoliophyta|Rep: AT5g12040/F14F18_210 - Arabidopsis
thaliana (Mouse-ear cress)
Length = 369
Score = 64.5 bits (150), Expect = 2e-09
Identities = 58/209 (27%), Positives = 90/209 (43%), Gaps = 12/209 (5%)
Frame = +3
Query: 114 ITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTG--- 284
+T + +K I AA++ ++ L E WN P++ + +AE + G
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPYS-----NDSFPVYAEEIDAGGDA 151
Query: 285 -PSTVFLAELAVKYDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH----- 443
PST L+E++ + + II I ER GD ++NT V G++ KHRK H
Sbjct: 152 SPSTAMLSEVSKRLKITIIGGSIPERV---GDRLYNTCCVFGSDGELKAKHRKIHLFDID 208
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
IP F ES G T + +T G++ I ICY +++ GA ++ P A
Sbjct: 209 IPGKITFMESKTLTAGET-PTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGA 267
Query: 624 TVSGLSEHLWAVEARNAAIANSYY--TCA 704
W + R A N Y TC+
Sbjct: 268 FNMTTGPLHWELLQRARATDNQLYVATCS 296
>UniRef50_Q54JM9 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 328
Score = 64.1 bits (149), Expect = 3e-09
Identities = 50/191 (26%), Positives = 82/191 (42%), Gaps = 5/191 (2%)
Frame = +3
Query: 153 KIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMV 332
K I AA ++ L E +N P++ T EK + E V L+E A + +
Sbjct: 75 KHIDEAAKNGAKLISLPECFNSPYSTSTFEKYSETEDGETVKK------LSEAAKRNQIF 128
Query: 333 IISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFEGNT 497
++ + D I+NT + N+ G+V+ KHRK H +P F ES G++
Sbjct: 129 LVGGSIPEIDKATGKIYNTCFIFNDKGEVVKKHRKIHLFDIDVPNKIRFKESETLTPGDS 188
Query: 498 GHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAA 677
V + Y K+ + ICY P +++ GA+ + P A W + R A
Sbjct: 189 -FSVVDIGYCKIGVAICYDIRFPELAMLYSKMGAKFLIYPGAFNMVTGPAHWELLQRGRA 247
Query: 678 IANSYYTCAIN 710
+ N + AI+
Sbjct: 248 VDNQVFVAAIS 258
>UniRef50_Q2NHR0 Cluster: Predicted amidohydrolase; n=1;
Methanosphaera stadtmanae DSM 3091|Rep: Predicted
amidohydrolase - Methanosphaera stadtmanae (strain DSM
3091)
Length = 274
Score = 64.1 bits (149), Expect = 3e-09
Identities = 49/193 (25%), Positives = 88/193 (45%), Gaps = 5/193 (2%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
E ++I A++ ++ L E +N P+ + + ++ E T + + ++A +
Sbjct: 22 EHAIQLIKKASSNGAKLITLPEMFNTPY-----DNSKFIEYCEEETTSKTLNSMQDIARE 76
Query: 321 YDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVG----DFNESTYYF 485
++ + S I E++ H ++NTA +IN GK+IGKHRK H+ + F ES
Sbjct: 77 ENIYLQSGSIPEKESNH---LYNTAYLINPKGKIIGKHRKMHMFDIDTDNMKFTESDTLT 133
Query: 486 EGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEA 665
G++ + +T ++I ICY P W + N ++I+ P A W
Sbjct: 134 PGDSVTTI-KTPLANISIAICYDIRFPELWTLMNKNNSDIILLPGAFNKTTGPLHWETLI 192
Query: 666 RNAAIANSYYTCA 704
+ AI N Y A
Sbjct: 193 KARAIDNQCYVVA 205
>UniRef50_A3H7D3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Caldivirga
maquilingensis IC-167|Rep: Nitrilase/cyanide hydratase
and apolipoprotein N-acyltransferase - Caldivirga
maquilingensis IC-167
Length = 279
Score = 63.7 bits (148), Expect = 4e-09
Identities = 49/149 (32%), Positives = 70/149 (46%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
AEP L G S L E+A + II+ I ERD G ++N+AV I E G ++ +RK H
Sbjct: 59 AEP-LDGKSIGELTEIAREGKCTIITGIAERDKDTG-VVYNSAVAIGENG-LMALYRKRH 115
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+P G F+ES Y+ G PVF K + ICY +P + GA + SA
Sbjct: 116 LPSYGVFDESRYFGVGRGDAPVFSMNGTKAGLAICYDAFYPEVSRSLMLKGARVQVYISA 175
Query: 624 TVSGLSEHLWAVEARNAAIANSYYTCAIN 710
+S + R A+ N + +N
Sbjct: 176 -APDMSRPHFETFIRARAMENVSFVIYVN 203
>UniRef50_Q6N746 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=11;
Proteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Rhodopseudomonas
palustris
Length = 579
Score = 63.3 bits (147), Expect = 6e-09
Identities = 50/162 (30%), Positives = 81/162 (50%)
Frame = +3
Query: 228 FCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINE 407
+C ++ F EP+ G +T AELA K+D I+ + E D+ +N+AV+I
Sbjct: 51 YCWYDRAEVAPFVEPI-PGATTARFAELARKHDCYIVVGLPEVDE--DGIYYNSAVLIGP 107
Query: 408 FGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFG 587
G +IG+HRK H P + +E + G+ + VF+T G++A+ IC H +
Sbjct: 108 EG-LIGRHRKTH-PYI---SEPKWSAAGDLHNQVFDTPIGRIALLICMDIHFVETARLMA 162
Query: 588 INGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
+ GA+I+ + S + L+E A + A NS Y NR
Sbjct: 163 LGGADIICHIS---NWLAERTPAPYWISRAFENSCYVIESNR 201
Score = 42.7 bits (96), Expect = 0.009
Identities = 34/114 (29%), Positives = 58/114 (50%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
GP+T LA LA + + ++ + ERD GD ++N+AV+I G I +RK H+
Sbjct: 349 GPATDRLAALASELSLYLVCGLAERD---GDILYNSAVLIAPDG-TITTYRKTHLTE--- 401
Query: 462 FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
NE + G++ V +T G+V + I + P + + G +I+ P+A
Sbjct: 402 -NERGWAQPGDS-FVVCDTPLGRVGLLIGHDAIFPEAGRVLALRGCDIIACPAA 453
>UniRef50_A6TL48 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Clostridiaceae|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Alkaliphilus
metalliredigens QYMF
Length = 296
Score = 63.3 bits (147), Expect = 6e-09
Identities = 42/151 (27%), Positives = 75/151 (49%)
Frame = +3
Query: 258 DFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRK 437
+ EP+ G T + +LA + ++ P+ ER + ++N++++I++ G++IGK+RK
Sbjct: 62 EILEPI-PGRHTRDIQKLAKELGTHVVFPLYERGKNKRE-VFNSSLMIDDRGEIIGKYRK 119
Query: 438 NHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNP 617
H P + E + V +TK GK+ + ICY P + + GAEI+ P
Sbjct: 120 TH-PFPTERKEGGGWTTPGNETVVVDTKLGKIGMIICYDGDFPELSRVLALKGAEIITRP 178
Query: 618 SATVSGLSEHLWAVEARNAAIANSYYTCAIN 710
SA + S +W + + A N Y +N
Sbjct: 179 SALLR--SFEIWEMTNKARAYDNHVYVLGVN 207
>UniRef50_A1HQ26 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Thermosinus
carboxydivorans Nor1|Rep: Nitrilase/cyanide hydratase
and apolipoprotein N-acyltransferase - Thermosinus
carboxydivorans Nor1
Length = 258
Score = 63.3 bits (147), Expect = 6e-09
Identities = 48/184 (26%), Positives = 83/184 (45%)
Frame = +3
Query: 159 ISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVII 338
++ A + +++ L E W +A RE W + E G + ++ ++ KY II
Sbjct: 25 LAQEGAARADVVVLPEIWTTGYAL--REVDKWAEDVE----GLTISEMSNISRKYGAYII 78
Query: 339 SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFET 518
+ + ++N AVVI G V ++RK H+ + E ++ G+ F
Sbjct: 79 AGSIPLRK--NGKVYNGAVVIGPDGNVAAEYRKIHLFSM--MGEERFFAAGDR-RCTFNL 133
Query: 519 KYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYT 698
K I ICY P + + ++GA+IVF P+ + EH W + +R AI N +
Sbjct: 134 KGVTAGIAICYDLRFPELFRVLALDGAQIVFLPAEWPTARGEH-WHLLSRTRAIENQVFL 192
Query: 699 CAIN 710
C +N
Sbjct: 193 CVVN 196
>UniRef50_A6CCB9 Cluster: Predicted amidohydrolase; n=1;
Planctomyces maris DSM 8797|Rep: Predicted
amidohydrolase - Planctomyces maris DSM 8797
Length = 282
Score = 62.9 bits (146), Expect = 8e-09
Identities = 51/193 (26%), Positives = 91/193 (47%)
Frame = +3
Query: 135 IFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELA 314
I EK+++ +A A+ V C +C + +AE + GPST L E+
Sbjct: 22 IIEKIKETAAAGASLTVFPECALTG------YCFASLEEALPYAESI-PGPSTDRLQEIC 74
Query: 315 VKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGN 494
+ + ++ +LE+ + ++N AV+I G V+G +RK H+P +G +T G+
Sbjct: 75 RELNHSVVVGMLEQAE---QGVYNAAVLITPEG-VLGSYRKIHLPYLGVDRFAT---PGD 127
Query: 495 TGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNA 674
V+ + +NICY P + + I GA+++ P+ +G + H+
Sbjct: 128 RDFAVYSHPEANIGLNICYDSAFPESSRIMTIEGADLIVLPTNWPTG-ANHVAEHAINTR 186
Query: 675 AIANSYYTCAINR 713
++ N Y CAINR
Sbjct: 187 SMENGIYYCAINR 199
>UniRef50_A7DPX6 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Crenarchaeota|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Candidatus
Nitrosopumilus maritimus SCM1
Length = 268
Score = 62.9 bits (146), Expect = 8e-09
Identities = 50/189 (26%), Positives = 82/189 (43%), Gaps = 1/189 (0%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
+K+ I AA++ LC + M + ++ + AE + G +A A +
Sbjct: 19 KKIISFIEKAASKNAT-LCAFPEFMMFYTNSSQTPKQLATLAETI-NGNFVNTIANTAKE 76
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
+ ++ E+ D +++T+ VI++ GKVI +RK H+ F ES G+
Sbjct: 77 NHVQVVGSFYEKSR-KKDRVYDTSFVIDKTGKVISTYRKIHLYDALGFRESDKMASGSKI 135
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSG-LSEHLWAVEARNAA 677
+T GKV + ICY P G+E++ PSA V G + E W + A
Sbjct: 136 AKPVKTTIGKVGMMICYDLRFPEMSRSLAAAGSEVLVAPSAWVKGNMKEEHWITINKTRA 195
Query: 678 IANSYYTCA 704
I N Y A
Sbjct: 196 IENGCYVIA 204
>UniRef50_Q74H63 Cluster: Hydrolase, carbon-nitrogen family; n=8;
Desulfuromonadales|Rep: Hydrolase, carbon-nitrogen
family - Geobacter sulfurreducens
Length = 259
Score = 61.3 bits (142), Expect = 2e-08
Identities = 51/189 (26%), Positives = 85/189 (44%), Gaps = 1/189 (0%)
Frame = +3
Query: 147 VQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVF-LAELAVKY 323
VQK + A++ + L E W+ +A+ + E P V L L+ +
Sbjct: 26 VQKALRRLASQGCRLAVLPEMWSTGYAYK--------ELNELAKRTPEVVAELGRLSREL 77
Query: 324 DMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGH 503
+MVI+ + E HG+ ++NTA V++ G+++G +RK H+ + + S +G
Sbjct: 78 EMVIVGSMPEP---HGEKVFNTAYVLDR-GELLGSYRKIHLFSLMGEDRS---LDGGDRW 130
Query: 504 PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIA 683
V +T G++ + ICY P + GAEI+ P+ EH W R AI
Sbjct: 131 LVVDTHVGRLGVFICYDLRFPELARRLAVEGAEIIVVPAEWPKPREEH-WRALLRARAIE 189
Query: 684 NSYYTCAIN 710
N + A N
Sbjct: 190 NQLFVVAAN 198
>UniRef50_Q44185 Cluster: N-carbamoyl-D-amino acid hydrolase; n=10;
Proteobacteria|Rep: N-carbamoyl-D-amino acid hydrolase -
Agrobacterium tumefaciens
Length = 304
Score = 61.3 bits (142), Expect = 2e-08
Identities = 27/84 (32%), Positives = 49/84 (58%), Gaps = 8/84 (9%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRVGDFN--------ESTYYFEGNTGHPVFETKYGKVA 536
+NT++++++ GK++GK+RK H+P ++ E Y+ G+ G PV++ K+
Sbjct: 109 FNTSILVDKSGKIVGKYRKIHLPGHKEYEAYRPFQHLEKRYFEPGDLGFPVYDVDAAKMG 168
Query: 537 INICYGRHHPLNWLMFGINGAEIV 608
+ IC R P W + G+ GAEI+
Sbjct: 169 MFICNDRRWPETWRVMGLKGAEII 192
>UniRef50_A2BNC1 Cluster: Predicted amidohydrolase; n=1;
Hyperthermus butylicus DSM 5456|Rep: Predicted
amidohydrolase - Hyperthermus butylicus (strain DSM 5456
/ JCM 9403)
Length = 269
Score = 60.5 bits (140), Expect = 4e-08
Identities = 36/124 (29%), Positives = 63/124 (50%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
AEP L GP F A LA +Y + +++ + E+ G +NTA +I G+++ +RK H
Sbjct: 63 AEP-LEGPWIGFFARLAREYSVHVVATLYEKSKAGGKP-YNTAALIAPTGELLAVYRKIH 120
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+ + ES Y+ G + K ++A+ +C+ P + + + GAE+V P+A
Sbjct: 121 LFDAYGYRESDYFMPGAEPAKLATIKGFRIALAVCFDLRFPELFRTYALQGAELVAVPAA 180
Query: 624 TVSG 635
G
Sbjct: 181 WYRG 184
>UniRef50_Q4P4D1 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 373
Score = 60.1 bits (139), Expect = 5e-08
Identities = 45/144 (31%), Positives = 68/144 (47%), Gaps = 6/144 (4%)
Frame = +3
Query: 285 PSTVFLAELAVKYDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH-----I 446
PS L+E A + ++V++ + ERDD+ G+ I+N++ V NE G++I HRK H I
Sbjct: 143 PSLKMLSETAREANVVLVGGSVPERDDLTGN-IYNSSCVFNEKGQLISIHRKLHLFDIDI 201
Query: 447 PRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSAT 626
P F ES G +F+ G+ + ICY P ++ G GA + P A
Sbjct: 202 PGKMTFQESE-TLAGGDRVTLFDCSLGRFGLGICYDLRFPEPAMIAGRLGAGCIIYPGAF 260
Query: 627 VSGLSEHLWAVEARNAAIANSYYT 698
+ W + R A N YT
Sbjct: 261 NTTTGPVSWELLLRARATDNQVYT 284
>UniRef50_A0TTW8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=5;
Proteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Burkholderia
cenocepacia MC0-3
Length = 299
Score = 59.7 bits (138), Expect = 7e-08
Identities = 46/154 (29%), Positives = 78/154 (50%)
Frame = +3
Query: 159 ISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVII 338
I AA ++ L E + + F R++ AE V GP+ +A + ++ I+
Sbjct: 42 IETAARNGAALIVLPELASSGYVFEDRDEA--LALAELVPDGPTARAFEAIARRLNVHIV 99
Query: 339 SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFET 518
S I ERD G ++N+A+ G +G +RK H+ D NE ++ G+ G PVF+T
Sbjct: 100 SGIAERD---GARLYNSALFAGPGGH-LGVYRKLHL---WD-NEKRFFEPGDRGVPVFDT 151
Query: 519 KYGKVAINICYGRHHPLNWLMFGINGAEIVFNPS 620
G++A+ ICY P + + + GA++V P+
Sbjct: 152 PLGRIAMAICYDVWFPETFRLAVMQGADLVCVPT 185
>UniRef50_A2XD42 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 349
Score = 59.7 bits (138), Expect = 7e-08
Identities = 49/180 (27%), Positives = 81/180 (45%), Gaps = 10/180 (5%)
Frame = +3
Query: 114 ITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTG--- 284
+T + + ++ I AAAA ++ L E WN P++ + ++AE + G
Sbjct: 55 VTADKARNIARAREAIEAAAAGGAKLVLLPEIWNGPYS-----NDSFPEYAEDIEAGGDA 109
Query: 285 -PSTVFLAELAVKYDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH----- 443
PS ++E+A + ++ I ER G+ ++NT V G++ GKHRK H
Sbjct: 110 APSFSMMSEVARSLQITLVGGSISERS---GNKLYNTCCVFGSDGELKGKHRKIHLFDID 166
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
IP F ES G V +T G++ I ICY +++ GA ++ P A
Sbjct: 167 IPGKITFKESKTLTAGQ-DLTVVDTDVGRIGIGICYDIRFQELAMLYAARGAHLLCYPGA 225
>UniRef50_Q6TGW8 Cluster: Nit protein 2; n=22; Fungi/Metazoa
group|Rep: Nit protein 2 - Danio rerio (Zebrafish)
(Brachydanio rerio)
Length = 277
Score = 58.8 bits (136), Expect = 1e-07
Identities = 48/189 (25%), Positives = 82/189 (43%), Gaps = 5/189 (2%)
Frame = +3
Query: 144 KVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKY 323
+ Q +++ AA + ++ L E +N P+ + ++AE + G ST L+E A K
Sbjct: 23 RAQTLVTEAAGQGAKVVVLPECFNSPYGTGFFK-----EYAEKI-PGESTQVLSETAKKC 76
Query: 324 DMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFE 488
+ ++ + +D G ++NT V G ++ HRK H +P F ES
Sbjct: 77 GIYLVGGSIPEED--GGKLYNTCSVFGPDGTLLVTHRKIHLFDIDVPGKIRFQESETLSP 134
Query: 489 GNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEAR 668
G + +FET Y KV + ICY ++ G +++ P A W + R
Sbjct: 135 GKS-LSMFETPYCKVGVGICYDIRFAELAQIYAKKGCQLLVYPGAFNMTTGPAHWELLQR 193
Query: 669 NAAIANSYY 695
A+ N Y
Sbjct: 194 GRAVDNQVY 202
>UniRef50_Q0RPB5 Cluster: Putative methylthioribose recycling
protein; n=1; Frankia alni ACN14a|Rep: Putative
methylthioribose recycling protein - Frankia alni
(strain ACN14a)
Length = 262
Score = 58.8 bits (136), Expect = 1e-07
Identities = 53/195 (27%), Positives = 92/195 (47%), Gaps = 2/195 (1%)
Frame = +3
Query: 132 AIFEKVQKIISAAAAEQVNILCLQEAWNMP-FAFCTREKQPWCDFAEPVLTGPSTVFLAE 308
++ ++V+++++ + +++ L E W F F + Q AEP LTGP+ L E
Sbjct: 12 SVADRVRRVLADLRSTDADLVVLPELWATGYFRFDAYQAQ-----AEP-LTGPTLTALRE 65
Query: 309 LAVKYDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYF 485
+A + +++ ++ER D + NT +I G ++ +RK H+ G +E+
Sbjct: 66 VARERRFHLVAGSLVERAD--DGRLHNTTALIGPGGDILHTYRKIHLFGYGS-DEARLLT 122
Query: 486 EGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEA 665
G T V T+ G + + CY P + + G GA++V SA + EH W V
Sbjct: 123 PGTTVDAV-RTELGCIGLATCYDLRFPELFRLLGDAGADLVAVVSAWPAARLEH-WRVLT 180
Query: 666 RNAAIANSYYTCAIN 710
R AI N + A N
Sbjct: 181 RARAIENQVHLVACN 195
>UniRef50_A0BLB1 Cluster: Chromosome undetermined scaffold_114,
whole genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_114,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 284
Score = 58.4 bits (135), Expect = 2e-07
Identities = 51/203 (25%), Positives = 96/203 (47%), Gaps = 12/203 (5%)
Frame = +3
Query: 132 AIFEKVQKIISAAAA-------EQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPS 290
A+FE QKI+ AA ++ ++ L E +N F +K DF++ +
Sbjct: 15 AVFETKQKILEGVAASIRDCVQKECKVIFLGEFFNTIFETNQLKKNAE-DFSDKN-NRET 72
Query: 291 TVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNE 470
+ +L+ ++ ++II + E D ++N A+ N+ GK++G++RK H+ V D
Sbjct: 73 YELMKQLSEEFQIMIIGGLPEVAD---GKLFNAALAFND-GKLVGQYRKCHLFDV-DIPG 127
Query: 471 STYYFEGNT-----GHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSG 635
+FE NT + +F+++YG+ + ICY P+ + G +++ PSA
Sbjct: 128 GITHFESNTFGSGNDYCIFDSQYGRYGLGICYDIRFPIYSQVMRDQGCQVLSFPSAFNQT 187
Query: 636 LSEHLWAVEARNAAIANSYYTCA 704
W + R+ A+ N Y +
Sbjct: 188 TGPLHWELLNRSRALDNQVYVAS 210
>UniRef50_A4SZC4 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase precursor; n=1;
Polynucleobacter sp. QLW-P1DMWA-1|Rep: Nitrilase/cyanide
hydratase and apolipoprotein N-acyltransferase precursor
- Polynucleobacter sp. QLW-P1DMWA-1
Length = 622
Score = 58.0 bits (134), Expect = 2e-07
Identities = 46/163 (28%), Positives = 77/163 (47%), Gaps = 1/163 (0%)
Frame = +3
Query: 144 KVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQ-PWCDFAEPVLTGPSTVFLAELAVK 320
K+ I + AA ++ E + F + T E+ P D G +T ++A K
Sbjct: 42 KMADISADAAKNGAKLIVFPEMASTGFLYMTLEQAGPNVD----TFPGKATAAFGQVAQK 97
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
Y+ I +E D G +N+A ++ G G +RK+ + VGD N + GN G
Sbjct: 98 YNTYIAWGYIELDPKTG-VAYNSAAIVGPNG-FSGNYRKHQLA-VGDDN--LFRAPGNIG 152
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATV 629
PVF T GK+A+ +CY + L+ + A+I+ P+A++
Sbjct: 153 FPVFNTPIGKIALLVCYDDSQLQSLLLPALRNADIIAYPTASL 195
Score = 41.1 bits (92), Expect = 0.026
Identities = 30/93 (32%), Positives = 49/93 (52%)
Frame = +3
Query: 261 FAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKN 440
FAEP L G S + LA K+ + ++ + E D + TA++ + GK IG +RK+
Sbjct: 373 FAEP-LNGKSYNIASSLAKKFQVNLLFSMPEITD---GKYYETAILFDYTGKQIGLYRKS 428
Query: 441 HIPRVGDFNESTYYFEGNTGHPVFETKYGKVAI 539
H+ + E T+ GN PVF + G++A+
Sbjct: 429 HLNDI----EKTWATAGNE-LPVFNSSIGRIAV 456
>UniRef50_Q6L0F7 Cluster: Carbon-nitrogen hydrolase family; n=2;
Thermoplasmatales|Rep: Carbon-nitrogen hydrolase family
- Picrophilus torridus
Length = 256
Score = 58.0 bits (134), Expect = 2e-07
Identities = 48/192 (25%), Positives = 91/192 (47%), Gaps = 1/192 (0%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
EK++K AA+ +++ E F F + +K+ + AEP+ + +++ +
Sbjct: 20 EKLRKYTEIAASNGADLIVFPEY----FMFYSNDKKYLNENAEPI----NGIWVKNVIKI 71
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
++ IS I+ ++++ + +++TAV I+ G V G +RK + + ES Y GN
Sbjct: 72 FNENSISGIVCINELNDNNVFDTAVYIS--GDVKGYYRKKMLYDAFGYRESDIYKSGNGP 129
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSG-LSEHLWAVEARNAA 677
++ I ICY P + + NGA+++ PS SG + E W R A
Sbjct: 130 FNLYRINDISFGILICYEIRFPELFRNYSKNGADMIIIPSGWFSGPVKEEQWLSLLRARA 189
Query: 678 IANSYYTCAINR 713
+ N+ Y + N+
Sbjct: 190 LENTVYIASSNQ 201
>UniRef50_Q8ZVX6 Cluster: Nitrilase, conjectural; n=4;
Pyrobaculum|Rep: Nitrilase, conjectural - Pyrobaculum
aerophilum
Length = 258
Score = 57.6 bits (133), Expect = 3e-07
Identities = 36/133 (27%), Positives = 63/133 (47%), Gaps = 1/133 (0%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
LA++A + + LER ++NT V+++ GK +G +RK H+ + ES
Sbjct: 61 LAKIAAETGAYVAGGFLERGP--RPKVFNTTVLVSPAGKAVGTYRKTHLFDAYGYKESEA 118
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSG-LSEHLWA 656
G +F+ + K+ +C+ P + + GA++V P+A SG L E +
Sbjct: 119 VEPGGELSGIFDVRQIKIGFAVCFELRFPEVFRELALGGAQLVAVPAAWYSGPLKEEILH 178
Query: 657 VEARNAAIANSYY 695
V AR A+ N +
Sbjct: 179 VLARARAVENGVF 191
>UniRef50_Q1QTM0 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Chromohalobacter
salexigens DSM 3043|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Chromohalobacter
salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB13768)
Length = 260
Score = 57.2 bits (132), Expect = 4e-07
Identities = 40/152 (26%), Positives = 74/152 (48%)
Frame = +3
Query: 258 DFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRK 437
+ AEPV GP AELA ++++ ++ + ER + N+AV+I++ G+ I + K
Sbjct: 55 ELAEPV-GGPIAQRAAELAAEHELFLLFGLAERQA--DGRLTNSAVLIDDRGERIATYHK 111
Query: 438 NHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNP 617
+ ++ +F V ET+ G++ + ICY P GA+++ +P
Sbjct: 112 RQL-----WDREHAFFAAGEDCCVVETRLGRLGLMICYDNEFPEVARALATQGAQVILSP 166
Query: 618 SATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
+A + +E A++ R A+ N + INR
Sbjct: 167 TANMVPNAER-QALQIRARALDNQCFVACINR 197
>UniRef50_Q1AWK1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=4; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Rubrobacter xylanophilus (strain DSM
9941 / NBRC 16129)
Length = 276
Score = 57.2 bits (132), Expect = 4e-07
Identities = 49/190 (25%), Positives = 86/190 (45%), Gaps = 5/190 (2%)
Frame = +3
Query: 150 QKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDM 329
+ +I AAA ++ L E W+ C ++ + + AEP+ GP+T FL LA + +
Sbjct: 27 EALIREAAAAGATLVALPELWS-----CHGLEEVYRENAEPI-PGPTTEFLGSLARELGI 80
Query: 330 VIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNT--G 500
++ ILER + + NT+ + G ++ +RK H+ V + + Y N G
Sbjct: 81 YLLGGSILERVS-GSERLGNTSTLYAPDGSLVAVYRKVHLFDV-EVSGRRYLESANIAPG 138
Query: 501 HPVFETKYGKVAI--NICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNA 674
K G V + ++CY P + + + GAE++ P+A + W + R
Sbjct: 139 GEAVAAKAGPVTVGLSVCYDVRFPELYRLLALRGAEVLAVPAAFTLQTGKDHWELLLRAR 198
Query: 675 AIANSYYTCA 704
A+ N Y A
Sbjct: 199 AVENQAYVLA 208
>UniRef50_A1HPP3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Thermosinus
carboxydivorans Nor1|Rep: Nitrilase/cyanide hydratase
and apolipoprotein N-acyltransferase - Thermosinus
carboxydivorans Nor1
Length = 259
Score = 57.2 bits (132), Expect = 4e-07
Identities = 38/144 (26%), Positives = 67/144 (46%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
GP+ L + A + I+ + +I ++NT VI+ G+V+GK+ K H+ V
Sbjct: 61 GPTVKMLQQFAKDNGVEIVGGSIA--EIRDGKVYNTIYVIDSAGEVVGKYSKIHL--VPM 116
Query: 462 FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLS 641
+E Y G+ +F+ +GK +CY + GAE++F P+ +
Sbjct: 117 MDEEKYLTPGDR-QGLFDLSFGKAGGIVCYDLRFTELTRALALKGAEVLFIPAEWPAIRG 175
Query: 642 EHLWAVEARNAAIANSYYTCAINR 713
H W + ++ AI N + A+NR
Sbjct: 176 RH-WLILSQARAIENQMFVVAVNR 198
>UniRef50_Q5KJU9 Cluster: Hydrolase, putative; n=1; Filobasidiella
neoformans|Rep: Hydrolase, putative - Cryptococcus
neoformans (Filobasidiella neoformans)
Length = 301
Score = 56.4 bits (130), Expect = 7e-07
Identities = 50/192 (26%), Positives = 78/192 (40%), Gaps = 14/192 (7%)
Frame = +3
Query: 162 SAAAAEQVNILCLQEAWNMPFAFCT----REKQP-----WCDFAEPVLTGPSTVFLAELA 314
+AA++ + ++ L E WN P+A + EK P W E G + L E+A
Sbjct: 37 AAASSPKPQLIVLPEIWNSPYAVSSFREYSEKVPEVGSKWKSLKEGE-EGETIKALREMA 95
Query: 315 VKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTY 479
+I + D D I+NT V + G ++ H+K H IP F ES
Sbjct: 96 RSSGCWLIGGSIPERDEKTDNIYNTCTVYDPEGTLVAVHQKVHLFDIDIPGKQTFKESDT 155
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAV 659
G + F T +GK+ + ICY P ++ G + P+A + W +
Sbjct: 156 -LTGGSHLTTFTTPFGKIGLGICYDIRFPEMAMIAARQGCIAMIYPAAFNTTTGPMHWTL 214
Query: 660 EARNAAIANSYY 695
R A+ N Y
Sbjct: 215 LQRARAVDNEIY 226
>UniRef50_A5FKF8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=5; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Flavobacterium johnsoniae UW101
Length = 321
Score = 56.0 bits (129), Expect = 9e-07
Identities = 41/162 (25%), Positives = 80/162 (49%), Gaps = 1/162 (0%)
Frame = +3
Query: 147 VQKIISAAAAEQVNILCLQEAWNMPFAFC-TREKQPWCDFAEPVLTGPSTVFLAELAVKY 323
++K+ A+ E +++ E + F + ++ D AE + +G S + L E+A K
Sbjct: 25 IEKLSQKASIEGCDVISFHECSITGYTFARSLSREQMLDLAELIPSGESILKLTEIAKKN 84
Query: 324 DMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGH 503
D+VI++ + E+D+ + ++ V +++ G ++ K+RK H P + Y +
Sbjct: 85 DIVILAGLFEKDE--NNNLFKAQVCVDKNG-LVAKYRKLH-PFINP------YLTAGDRY 134
Query: 504 PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATV 629
+FE + K I ICY + N + GA+I+F P T+
Sbjct: 135 CIFEIEGWKCGILICYDNNIIENVRATKLLGADIIFMPHVTM 176
>UniRef50_Q8WUF0 Cluster: Nitrilase family member 2; n=28; cellular
organisms|Rep: Nitrilase family member 2 - Homo sapiens
(Human)
Length = 276
Score = 55.6 bits (128), Expect = 1e-06
Identities = 56/216 (25%), Positives = 95/216 (43%), Gaps = 6/216 (2%)
Frame = +3
Query: 66 RLGLIQHSIA-ISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTRE 242
RL LIQ I+ I +DN +T + I AA + I+ L E +N P+
Sbjct: 5 RLALIQLQISSIKSDN-VT--------RACSFIREAATQGAKIVSLPECFNSPYG----- 50
Query: 243 KQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVI 422
+ + ++AE + G ST L+E+A + + +I + +D ++NT V G ++
Sbjct: 51 AKYFPEYAEKI-PGESTQKLSEVAKECSIYLIGGSIPEED--AGKLYNTCAVFGPDGTLL 107
Query: 423 GKHRKNH-----IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFG 587
K+RK H +P F ES G++ F+T Y +V + ICY ++
Sbjct: 108 AKYRKIHLFDIDVPGKITFQESKTLSPGDS-FSTFDTPYCRVGLGICYDMRFAELAQIYA 166
Query: 588 INGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYY 695
G +++ P A W + R+ A+ N Y
Sbjct: 167 QRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVY 202
>UniRef50_UPI0000E1FE2F Cluster: PREDICTED: similar to Nitrilase
family, member 2; n=2; Coelomata|Rep: PREDICTED: similar
to Nitrilase family, member 2 - Pan troglodytes
Length = 411
Score = 55.2 bits (127), Expect = 2e-06
Identities = 56/216 (25%), Positives = 94/216 (43%), Gaps = 6/216 (2%)
Frame = +3
Query: 66 RLGLIQHSIA-ISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTRE 242
RL LIQ I+ I +DN +T + I AA + I+ L E +N P+
Sbjct: 140 RLALIQLQISSIKSDN-VT--------RACSFIREAATQGAKIVSLPECFNSPYG----- 185
Query: 243 KQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVI 422
+ + ++AE + G ST L E+A + + +I + +D ++NT V G ++
Sbjct: 186 TKYFPEYAEKI-PGESTQKLCEVAKECSIYLIGGSIPEED--AGKLYNTCAVFGPDGTLL 242
Query: 423 GKHRKNH-----IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFG 587
K+RK H +P F ES G++ F+T Y +V + ICY ++
Sbjct: 243 AKYRKIHLFDIDVPGKITFQESKTLSPGDS-FSTFDTPYCRVGLGICYDMRFAELAQIYA 301
Query: 588 INGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYY 695
G +++ P A W + R+ A+ N Y
Sbjct: 302 QRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVY 337
>UniRef50_Q9KE11 Cluster: BH1047 protein; n=1; Bacillus
halodurans|Rep: BH1047 protein - Bacillus halodurans
Length = 271
Score = 55.2 bits (127), Expect = 2e-06
Identities = 40/140 (28%), Positives = 67/140 (47%)
Frame = +3
Query: 294 VFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNES 473
+FL ELA ++++ I++ + + + ++N A+V + G + ++ K H+ V +E
Sbjct: 67 LFLKELAREHNVNIVAGSIAKKE--KGKLYNRALVFDRRGHTVYQYDKIHL--VPMLSEP 122
Query: 474 TYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLW 653
Y G+ VFE + K+ + ICY P + GAEIVF + + H W
Sbjct: 123 DYLTGGDAAASVFELEGTKMGLVICYDLRFPELMRSLALEGAEIVFIVAEWPEARAVH-W 181
Query: 654 AVEARNAAIANSYYTCAINR 713
V R AI N Y + NR
Sbjct: 182 EVLQRARAIENQSYVISCNR 201
>UniRef50_A5V6Z2 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Sphingomonas
wittichii RW1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Sphingomonas
wittichii RW1
Length = 384
Score = 55.2 bits (127), Expect = 2e-06
Identities = 49/160 (30%), Positives = 73/160 (45%), Gaps = 15/160 (9%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISP-ILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPR 452
L GP L E+A +Y++ I ++ER D +NTA +I G+V+ ++ K HIP
Sbjct: 83 LDGPEMRRLGEVAKEYNLYIAGGGVVERVKEFPDRWFNTAFIIGPSGEVVLRYHKWHIPA 142
Query: 453 ---VGD-----FNESTYYFEGN--TGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAE 602
+G F+E F G+ T PV +T+ GK+ C+ P G NG E
Sbjct: 143 SIGLGTSPHDIFDEYKEVFGGDISTLFPVIDTEIGKLGTMTCHDGCTPEVSRALGYNGVE 202
Query: 603 IVFNPSA--TVSGLSE--HLWAVEARNAAIANSYYTCAIN 710
++ +P A V G+S+ W R A N Y N
Sbjct: 203 VICHPVALQEVEGVSQPWDFWMFTRRTRAHDNMAYVLGSN 242
>UniRef50_UPI000051A529 Cluster: PREDICTED: similar to Nitrilase and
fragile histidine triad fusion protein CG7067-PA; n=1;
Apis mellifera|Rep: PREDICTED: similar to Nitrilase and
fragile histidine triad fusion protein CG7067-PA - Apis
mellifera
Length = 304
Score = 54.8 bits (126), Expect = 2e-06
Identities = 43/150 (28%), Positives = 64/150 (42%), Gaps = 7/150 (4%)
Frame = +3
Query: 276 LTGPSTVFLAELA-VKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPR 452
L G + E+A + + + I E D + + I NT ++IN G+++ +RK H+
Sbjct: 85 LNGSTVTSYKEIAKINKIWLSLGGIHEALDNNREHISNTHILINSEGEIVSTYRKIHLFD 144
Query: 453 VGDFN------ESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFN 614
+ + N ES Y G P T GK+A++ICY P GAEI+
Sbjct: 145 MDNKNTGVRLMESDYVLPGQKIEPPISTPIGKLALSICYDMRFPELSFSLRNMGAEILTY 204
Query: 615 PSATVSGLSEHLWAVEARNAAIANSYYTCA 704
PSA W + R AI Y A
Sbjct: 205 PSAFTYQTGAAHWEILLRARAIETQCYVVA 234
>UniRef50_A3TQB8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Janibacter sp.
HTCC2649|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Janibacter sp.
HTCC2649
Length = 310
Score = 54.8 bits (126), Expect = 2e-06
Identities = 39/145 (26%), Positives = 68/145 (46%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRV 455
L GP T +A + +V+ ER G ++N +V+IN G+++G +RK H
Sbjct: 72 LPGPMTAPFQAVARELGIVLCVGTYERGPERG-IVYNASVLINSDGELLGVYRKTHPFCT 130
Query: 456 GDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSG 635
+ + G+T V +T G++ + IC+ +P + + GAEI+ PSA +
Sbjct: 131 EAVSGGGWVTPGDTV-TVCDTAIGRIGMIICFDGDYPELSRIQAVQGAEIICRPSALLR- 188
Query: 636 LSEHLWAVEARNAAIANSYYTCAIN 710
S +W + +R A N + N
Sbjct: 189 -SADIWELTSRARAYDNHVFVIGAN 212
>UniRef50_A2BKF1 Cluster: Predicted amidohydrolase; n=1;
Hyperthermus butylicus DSM 5456|Rep: Predicted
amidohydrolase - Hyperthermus butylicus (strain DSM 5456
/ JCM 9403)
Length = 272
Score = 54.8 bits (126), Expect = 2e-06
Identities = 52/187 (27%), Positives = 84/187 (44%), Gaps = 2/187 (1%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNM-PFAFCTREKQPWCDFAEPVLTGPSTVFLAELAV 317
E++++++S E +I+ L E N+ P E + AE P FL E++
Sbjct: 22 ERLRRLLSRYRVE-ADIIVLPEYGNVYPAGLRAAEVRAR---AENPKDSPFIRFLEEISS 77
Query: 318 KYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNT 497
+Y VI+S LER GD +++ V++ +V +RK + ES G
Sbjct: 78 EYTAVIVSGFLERS---GDCAYSSIVMVEPGKEVQVVYRKTVLFDALGVRESKSLCRGEQ 134
Query: 498 GHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSG-LSEHLWAVEARNA 674
PV E + +V +C+ P + GAE+V P+A G L E V AR+
Sbjct: 135 PPPVLEVRGVRVGFIVCFELRFPELARSLALRGAELVAVPAAWYRGNLKEEHLLVTARSR 194
Query: 675 AIANSYY 695
A+ N+ Y
Sbjct: 195 ALENTVY 201
>UniRef50_Q2JDM2 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=12;
Actinomycetales|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Frankia sp. (strain
CcI3)
Length = 404
Score = 54.0 bits (124), Expect = 3e-06
Identities = 52/190 (27%), Positives = 81/190 (42%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
++V++++ Q +++ L E W + R + AE LTGP+ L E A +
Sbjct: 22 DRVRRVLGEIRQTQADLVVLPELWVTGYFHFDRYEAE----AE-ALTGPTVTALREAARE 76
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
+++ + G ++NT V+I G + +RK H+ G E+ G T
Sbjct: 77 RGCHLVAGSIVERSADG-RLFNTTVLIGPDGMIRHAYRKVHLFGYGSA-EARLLTPGATV 134
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAI 680
V T+ G V + CY P + + GAEIV SA +H W V R AI
Sbjct: 135 GTV-PTELGIVGLATCYDLRFPELFRLLAEGGAEIVVVVSAWPLARLDH-WRVLTRTRAI 192
Query: 681 ANSYYTCAIN 710
N Y A N
Sbjct: 193 ENQVYLVACN 202
>UniRef50_A1IFF1 Cluster: Hydrolase, carbon-nitrogen family; n=1;
Candidatus Desulfococcus oleovorans Hxd3|Rep: Hydrolase,
carbon-nitrogen family - Candidatus Desulfococcus
oleovorans Hxd3
Length = 270
Score = 54.0 bits (124), Expect = 3e-06
Identities = 49/185 (26%), Positives = 77/185 (41%)
Frame = +3
Query: 159 ISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVII 338
I+ AA+ ++ L E W P F R A T ++ A ++ MVI
Sbjct: 31 IAGLAAQGADLAVLPELW--PCGFDNRHLA-----AHAAQTPRILEIVSAQAAEHSMVIA 83
Query: 339 SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFET 518
+ E D I NT VV++ G+ G++RK H+ G E ++ +G V +T
Sbjct: 84 GSVPEAGP---DGICNTLVVMDRDGREAGRYRKIHLFSAG--GEERFFAKGKAW-AVCDT 137
Query: 519 KYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYT 698
GK+ + ICY P + ++GA V P+ +H W + AI N +
Sbjct: 138 AAGKLGLMICYDLRFPELCRVLALDGAACVIVPAQWPEARIDH-WNALLKARAIENQLFV 196
Query: 699 CAINR 713
NR
Sbjct: 197 VGANR 201
>UniRef50_A0RYH6 Cluster: Amidohydrolase; n=1; Cenarchaeum
symbiosum|Rep: Amidohydrolase - Cenarchaeum symbiosum
Length = 269
Score = 54.0 bits (124), Expect = 3e-06
Identities = 46/187 (24%), Positives = 77/187 (41%), Gaps = 3/187 (1%)
Frame = +3
Query: 144 KVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPV--LTGPSTVFLAELAV 317
++ K +S AAA ++ E F T Q + A + GP +A+ A
Sbjct: 21 RIVKYVSEAAAGGAGLVAFPEF----MMFYTPPGQTPAELARLAENIDGPFVKSVADAAR 76
Query: 318 KYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNT 497
Y + ++ I ER G +++T+ ++ G ++ +RK H+ F ES G+
Sbjct: 77 DYSIEVVGTIYERSPRRG-RVYDTSFLLGRDGSLLSSYRKIHLYDALGFKESAKLAPGDR 135
Query: 498 GHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLS-EHLWAVEARNA 674
+ G + + ICY P +GA ++ PSA V G + E W R
Sbjct: 136 MTVPSGSSVGSLGMLICYDLRFPEAARTLASSGAGVIVAPSAWVQGKNKEDQWITMNRAR 195
Query: 675 AIANSYY 695
A+ N Y
Sbjct: 196 AMENGCY 202
>UniRef50_Q1PXD4 Cluster: Similar to N-carbamoyl-D-amino acid
hydrolase; n=1; Candidatus Kuenenia stuttgartiensis|Rep:
Similar to N-carbamoyl-D-amino acid hydrolase -
Candidatus Kuenenia stuttgartiensis
Length = 277
Score = 53.6 bits (123), Expect = 5e-06
Identities = 44/166 (26%), Positives = 74/166 (44%), Gaps = 5/166 (3%)
Frame = +3
Query: 222 FAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVI 401
F+F +E++ FAE TG FL + ++K+ + II + + NT +V
Sbjct: 45 FSFIGQEREN-ITFAEERETGEIVHFLKKFSMKHSVAIIGGSVPLRSSSKAKVTNTCLVF 103
Query: 402 NEFGKVIGKHRKNHIPRVGDFNESTYYFEGNT---GHPVFETK-YGKV-AINICYGRHHP 566
++ G +IG + K H+ ++ T Y E + G + K +G + + ICY P
Sbjct: 104 DQSGVIIGSYDKIHLFDF-HLDDKTVYRESHYVKHGKHIETVKLFGHIMGLCICYDLRFP 162
Query: 567 LNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
+ + G E++F PSA + W + R AI N Y A
Sbjct: 163 ELFRKLMLRGMEVLFAPSAFTMETGKDHWEILLRARAIENQCYVVA 208
>UniRef50_A0QPL8 Cluster: Hydrolase, carbon-nitrogen family protein;
n=6; Bacteria|Rep: Hydrolase, carbon-nitrogen family
protein - Mycobacterium smegmatis (strain ATCC 700084 /
mc(2)155)
Length = 330
Score = 53.6 bits (123), Expect = 5e-06
Identities = 42/163 (25%), Positives = 72/163 (44%), Gaps = 8/163 (4%)
Frame = +3
Query: 159 ISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVII 338
I AA E + L E + + T D AE + GP+ AE A + +
Sbjct: 47 IDRAAGEGAKAVFLPEITLLRYPADTPAGPNPGDVAEDLTGGPTFELAAEAARANGIFVH 106
Query: 339 SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEG------NTG 500
+ + E+ +NTA++++ G+++G+ RK HIP + E TY+ G
Sbjct: 107 ASLYEKAPAADGLGYNTAILVSPEGELVGRTRKMHIPISAGYYEDTYFRPGPARPSDGDP 166
Query: 501 HPVF--ETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+PV+ E ++ + C+ P + + GAEIV P+A
Sbjct: 167 YPVYSPEGLGARIGLPTCWDEWFPEVARCYSLGGAEIVVYPTA 209
>UniRef50_Q6N4F1 Cluster: Possible amidohydrolase; n=2;
Rhodopseudomonas palustris|Rep: Possible amidohydrolase
- Rhodopseudomonas palustris
Length = 557
Score = 53.2 bits (122), Expect = 6e-06
Identities = 39/153 (25%), Positives = 71/153 (46%), Gaps = 1/153 (0%)
Frame = +3
Query: 153 KIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWC-DFAEPVLTGPSTVFLAELAVKYDM 329
+ + AA + ++ E + + F + E C + AE + GP LA L+ K+ +
Sbjct: 29 RYVEDAARQGAELIVFPECMDTGYLFDSPEH---CRELAETLTDGPFVKALAALSRKHGV 85
Query: 330 VIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPV 509
I S I E D + I+NT ++ + G+V + K + ++ ++ G G PV
Sbjct: 86 YIASGITEWDPAK-EKIFNTGIMFDRKGEVACHYHKQFLAT----HDQNWFAFGERGCPV 140
Query: 510 FETKYGKVAINICYGRHHPLNWLMFGINGAEIV 608
ET GK+ + IC+ P + + GAE++
Sbjct: 141 VETDLGKIGLLICFDGRIPEIFRAMTMQGAEVI 173
Score = 50.8 bits (116), Expect = 3e-05
Identities = 33/120 (27%), Positives = 57/120 (47%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
+A+++ +Y +I +PI+ER ++ T V+I GK IG++RK H+ E
Sbjct: 360 VAKISARYGCLIAAPIVERA---AAGLYVTTVLIGSDGKEIGRYRKTHLTA-----EERK 411
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAV 659
+ +PVF+T +G++ + Y P I A+I+ P+A L AV
Sbjct: 412 WAVAGFDYPVFDTPFGRIGVMSGYDAVFPETSRCLAIGAADIILWPAALREPFERELLAV 471
>UniRef50_Q2S2E4 Cluster: Hydrolase, carbon-nitrogen family; n=1;
Salinibacter ruber DSM 13855|Rep: Hydrolase,
carbon-nitrogen family - Salinibacter ruber (strain DSM
13855)
Length = 281
Score = 53.2 bits (122), Expect = 6e-06
Identities = 53/202 (26%), Positives = 89/202 (44%), Gaps = 7/202 (3%)
Frame = +3
Query: 129 LAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAE 308
L + + + + S + + +++ L E + + F + K AEP+ G S L
Sbjct: 15 LEVDQNLAAVESLLRSVEADLIVLPELFTSGYFF--QSKDDLERVAEPIPNGKSVAALRG 72
Query: 309 LAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFE 488
A +++ + ERD GD +N+AVV+ G+V +RK H+ F E T FE
Sbjct: 73 WADSLGATLVAGLAERD---GDHFYNSAVVVRPDGRV-DTYRKVHL-----FYEETILFE 123
Query: 489 -GNTGHPVFE------TKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEH 647
G+ G VFE T Y ++ + +C+ + P + GA+++ +PS V
Sbjct: 124 AGDLGFRVFEEHTAAGTSY-RLGVMVCFDWYFPEAARTLALRGADVIAHPSNLVLPHCPD 182
Query: 648 LWAVEARNAAIANSYYTCAINR 713
V AR N +T NR
Sbjct: 183 SMPVRARE----NHVFTITANR 200
>UniRef50_A4SSL0 Cluster: Beta-ureidopropionase; n=1; Aeromonas
salmonicida subsp. salmonicida A449|Rep:
Beta-ureidopropionase - Aeromonas salmonicida (strain
A449)
Length = 277
Score = 52.8 bits (121), Expect = 8e-06
Identities = 40/163 (24%), Positives = 71/163 (43%), Gaps = 5/163 (3%)
Frame = +3
Query: 240 EKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKV 419
E+Q + D AE + GP LA A +Y + +++ + I +++V + G++
Sbjct: 47 ERQGYLDGAERIGEGPIQQQLAAWAKEYGIWLVAGAMPTAIPGSAHIHTSSLVFDPAGEL 106
Query: 420 IGKHRKNHIPRVGDFNESTYYFEGNTGHP-----VFETKYGKVAINICYGRHHPLNWLMF 584
G + K H+ V + Y E T P + ++ +G + ++ICY P +
Sbjct: 107 KGHYHKIHLFDVDVADNQGRYRESETFSPGQDCVLIDSPFGPLGLSICYDLRFPELYRQL 166
Query: 585 GINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
GA ++ P+A + E W R AI N Y A N+
Sbjct: 167 ARAGARVLLVPAAFTAVTGEAHWEPLLRARAIENQCYVVAANQ 209
>UniRef50_Q0S9Y1 Cluster: Possible nitrilase; n=4;
Actinomycetales|Rep: Possible nitrilase - Rhodococcus
sp. (strain RHA1)
Length = 270
Score = 52.4 bits (120), Expect = 1e-05
Identities = 49/190 (25%), Positives = 82/190 (43%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
E+V+ +++ A E+V+++ L E W + + + AE L G + LA +AV+
Sbjct: 21 ERVRNLLTGLA-ERVDLIVLPELWRVGY----NHFDDYSTAAE-TLGGGTVQVLAAVAVE 74
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
I + + G + NTAV+I G++ + K H+ D E+ G
Sbjct: 75 RQCYIHAGSIVEQGEEG-RLRNTAVLIGPDGQIHHHYSKVHVFGY-DSLEAQLLQPGTQI 132
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAI 680
H +T +G +A CY P W GA++V P+A EH W + A+
Sbjct: 133 HTT-DTPFGPIAATTCYDLRFPGLWTELVAAGAQLVIVPAAWPKARKEH-WRLLTSARAV 190
Query: 681 ANSYYTCAIN 710
N + A N
Sbjct: 191 DNQVFVIACN 200
>UniRef50_A3LZY2 Cluster: Aliphatic nitrilase; n=1; Pichia
stipitis|Rep: Aliphatic nitrilase - Pichia stipitis
(Yeast)
Length = 323
Score = 52.4 bits (120), Expect = 1e-05
Identities = 41/154 (26%), Positives = 70/154 (45%), Gaps = 8/154 (5%)
Frame = +3
Query: 111 PITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCD---FAEPV-- 275
P+ + A EKV +S AA++ N++ E + F K P + F + V
Sbjct: 15 PVMMNKEATMEKVFNGVSEAASKGANLIVFPETYVSAFPLWGACKAPIDNHHLFKQLVES 74
Query: 276 ---LTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI 446
+ GP L L + +V++ ER + +WN+ V+I+E G IG H + +
Sbjct: 75 SIYIDGPEISSLQSLCKELSVVVLLGFNERSRVSVGCLWNSYVLIDENG-TIGAHHRKLV 133
Query: 447 PRVGDFNESTYYFEGNTGHPVFETKYGKVAINIC 548
P F + ++ +G V ++KYGK+ IC
Sbjct: 134 PTF--FEKLSWANGDGSGLNVIDSKYGKIGCLIC 165
>UniRef50_A4WA35 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=12; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Enterobacter sp. 638
Length = 326
Score = 52.0 bits (119), Expect = 1e-05
Identities = 49/166 (29%), Positives = 71/166 (42%), Gaps = 6/166 (3%)
Frame = +3
Query: 147 VQKIISAAAAEQVNIL-----CLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAEL 311
++K I AA EQVNIL C+ W++P AEP+ PS + L
Sbjct: 28 IEKFIEQAALEQVNILVFPEMCITGYWHVPKLTAAEVSA----LAEPIAESPSLTLIRSL 83
Query: 312 AVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEG 491
A+K+ M+I ++ER D ++N V G + HRK H F
Sbjct: 84 AIKHQMLIGVGLIERAD--DGRLYNAYVACMPDG-TMHTHRKLHA-----FEHPA--ISS 133
Query: 492 NTGHPVFETKYG-KVAINICYGRHHPLNWLMFGINGAEIVFNPSAT 626
VF+T +G KV I IC+ + N + GA+I+ P T
Sbjct: 134 GDRFTVFDTPWGVKVGILICWDNNLVENVRATALLGADILLAPHQT 179
>UniRef50_A4BQN0 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Nitrococcus
mobilis Nb-231|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Nitrococcus mobilis
Nb-231
Length = 287
Score = 52.0 bits (119), Expect = 1e-05
Identities = 45/195 (23%), Positives = 78/195 (40%), Gaps = 4/195 (2%)
Frame = +3
Query: 132 AIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAEL 311
A E ++I+ A A +++ L E FAF R++ AEP GP FLAE
Sbjct: 21 ANLESADRLIAEAVAGGADLVALPEN----FAFVGRDETGKLAIAEPDDGGPIQSFLAER 76
Query: 312 AVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYY--- 482
A ++ + ++ + +V G+ ++ K H+ V + Y
Sbjct: 77 ARRHGIFLVGGTIPLHTSDQRRARAACLVYGPSGERCARYDKIHLFDVAVSADERYCESE 136
Query: 483 -FEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAV 659
+ +F+T + +V + +CY P + GAE++ PSA + W +
Sbjct: 137 TLQAGNNAVIFDTPFARVGLAVCYDLRFPELFRELVARGAELLVVPSAFTALTGAAHWEL 196
Query: 660 EARNAAIANSYYTCA 704
R A+ N Y A
Sbjct: 197 LVRTRAVENLCYLVA 211
>UniRef50_A3ZLM3 Cluster: Putative nitrilase; n=1; Blastopirellula
marina DSM 3645|Rep: Putative nitrilase -
Blastopirellula marina DSM 3645
Length = 258
Score = 52.0 bits (119), Expect = 1e-05
Identities = 46/197 (23%), Positives = 91/197 (46%), Gaps = 6/197 (3%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
+ +++I+ AA ++ L E +N + + AE + +GP+ V + + A+K
Sbjct: 12 QTAERLIAQAAERGAQLVVLPELFNY-----LGRLENLVEHAETI-SGPTAVRMRKAALK 65
Query: 321 YDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYY 482
+ + +++ ER + ++NT+++ + GK IG +RK H +P V +ES++
Sbjct: 66 HQIYLVAGSFAERSETES-RVFNTSLIFDPLGKQIGVYRKIHLFDIDLPDV-QVHESSFV 123
Query: 483 FEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVE 662
G+ + +T G VA ICY P + + + P+A + W +
Sbjct: 124 APGSE-VSLCQTALGGVAQAICYDLRFPEIVRSYDLEKVACLALPAAFTAKTGAAHWQIL 182
Query: 663 ARNAAIANSYYTCAINR 713
R+ AI N + A N+
Sbjct: 183 VRSRAIENQLFLIAANQ 199
>UniRef50_A0L7H1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Proteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Magnetococcus sp.
(strain MC-1)
Length = 275
Score = 52.0 bits (119), Expect = 1e-05
Identities = 43/194 (22%), Positives = 85/194 (43%), Gaps = 4/194 (2%)
Frame = +3
Query: 144 KVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKY 323
+ ++++ AA +L L E N F F EK+ +P GPS + A ++
Sbjct: 26 RAEQLLEEAATAGAKLLVLPE--NFSF-FGADEKEKLAHQEDPQ-HGPSLRMVQAFAQRH 81
Query: 324 DMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRV----GDFNESTYYFEG 491
+++ + D + N++ V+N+ G+V+ ++ K H+ V G+ +
Sbjct: 82 GAWVVAGSIPTDVGESQRVANSSFVVNDQGQVVARYDKIHLFDVTLNGGEGYRESDMIRA 141
Query: 492 NTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARN 671
+ V ++ +G++ ++ICY P + GAEI P+A + W + R
Sbjct: 142 GSQPVVVDSPFGRIGLSICYDLRFPELYRALTDAGAEIFTVPAAFTLTTGQVHWELLLRA 201
Query: 672 AAIANSYYTCAINR 713
A+ N + A N+
Sbjct: 202 RAVENFCHLLAPNQ 215
>UniRef50_Q17CS4 Cluster: Nitrilase, putative; n=3; Culicidae|Rep:
Nitrilase, putative - Aedes aegypti (Yellowfever
mosquito)
Length = 477
Score = 52.0 bits (119), Expect = 1e-05
Identities = 36/113 (31%), Positives = 51/113 (45%), Gaps = 4/113 (3%)
Frame = +3
Query: 378 IWNTAVVINEFGKVIGKHRKNHIPRVGD----FNESTYYFEGNTGHPVFETKYGKVAINI 545
I+NT +VI+ G+++ ++RK H+ V F ES G+ P ET G+V + I
Sbjct: 135 IYNTHIVIDNEGQLVAQYRKLHMFNVVTPEFKFRESETVRSGSELVPPIETPIGRVGLQI 194
Query: 546 CYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
CY + GAEI+ PSA W V R AI N + A
Sbjct: 195 CYDVRFAEASTLLRKQGAEILTYPSAFAVSTGRAHWEVLLRARAIENQCFVIA 247
>UniRef50_Q72HE8 Cluster: Beta-ureidopropionase; n=2; Thermus
thermophilus|Rep: Beta-ureidopropionase - Thermus
thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Length = 292
Score = 51.6 bits (118), Expect = 2e-05
Identities = 28/98 (28%), Positives = 50/98 (51%)
Frame = +3
Query: 333 IISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVF 512
++ ERD+ +N+A + +V+ HRK +P G F+E Y G F
Sbjct: 85 VVVGFYERDE---GAYYNSAAYLELPHRVVHVHRKVFLPTYGVFDEERYLARGRRVE-AF 140
Query: 513 ETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSAT 626
T++G+ A+ IC H + + ++GAE+++ PSA+
Sbjct: 141 RTRFGRAALLICEDFWHSITATIAALDGAEVIYVPSAS 178
>UniRef50_P46011 Cluster: Nitrilase 4; n=49; cellular organisms|Rep:
Nitrilase 4 - Arabidopsis thaliana (Mouse-ear cress)
Length = 355
Score = 51.6 bits (118), Expect = 2e-05
Identities = 40/145 (27%), Positives = 65/145 (44%), Gaps = 1/145 (0%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
GP LA +A KY + ++ ++ER+ G T++ T + + G +GKHRK +P
Sbjct: 115 GPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRKL-MPTA-- 168
Query: 462 FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHP-LNWLMFGINGAEIVFNPSATVSGL 638
+ F + PVF+T GK+ IC+ P L M+ G EI P+A
Sbjct: 169 LERCIWGFGDGSTIPVFDTPIGKIGAAICWENRMPSLRTAMYA-KGIEIYCAPTAD---- 223
Query: 639 SEHLWAVEARNAAIANSYYTCAINR 713
S W + A+ + + N+
Sbjct: 224 SRETWLASMTHIALEGGCFVLSANQ 248
>UniRef50_Q9ZMC7 Cluster: Putative; n=6; Campylobacterales|Rep:
Putative - Helicobacter pylori J99 (Campylobacter pylori
J99)
Length = 294
Score = 51.2 bits (117), Expect = 2e-05
Identities = 42/184 (22%), Positives = 84/184 (45%), Gaps = 9/184 (4%)
Frame = +3
Query: 186 NILCLQEAWNMPFAFCTREKQPWCDF-----AEPVLTGPSTVFLAELAVKYDMVIISPIL 350
N++ L E ++ + ++ DF E L + L++ A D I++ +
Sbjct: 45 NLIVLPELFDSGYCVNDKDADFGLDFKAIEHGEETLKNETLRALSDFAKSSDTHIVACSI 104
Query: 351 ERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYG- 527
E+++ ++++A +I GK++GKHRK ++ + + F+ + VF +G
Sbjct: 105 EKNN---KKLYDSAYIIPPKGKIVGKHRKIYL-----WGDEKSRFKRGKKYEVFTLDFGD 156
Query: 528 ---KVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYT 698
KV + ICY + + + GAE++ PSA + W + ++ A+ N +
Sbjct: 157 FSAKVGLQICYETGFGVGANLLVLQGAEVLIYPSA-FGKARAYNWDLLSKARALENGCFV 215
Query: 699 CAIN 710
CA N
Sbjct: 216 CACN 219
>UniRef50_Q8DCG5 Cluster: Predicted amidohydrolase; n=33;
Gammaproteobacteria|Rep: Predicted amidohydrolase -
Vibrio vulnificus
Length = 274
Score = 51.2 bits (117), Expect = 2e-05
Identities = 40/160 (25%), Positives = 71/160 (44%), Gaps = 6/160 (3%)
Frame = +3
Query: 243 KQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVI 422
++ + AEP+ GP +A+LA + + ++ I G + T++V G+ +
Sbjct: 49 REDYHQHAEPLGNGPLQQAMAQLAKRLAVTLV--IGSMPIRQGHDVTTTSLVFGPNGERL 106
Query: 423 GKHRKNHIPRV------GDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMF 584
G + K H+ V G + ES + G+ V T G++ ++ICY P +
Sbjct: 107 GHYSKLHMFDVEVSDGHGHYRESDSFLAGDRSS-VVATPIGRLGLSICYDVRFPALYQTL 165
Query: 585 GINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
GA+I+ P+A + E W + R AI N + A
Sbjct: 166 RQKGADILLVPAAFTAVTGEAHWEILLRARAIENQCWVIA 205
>UniRef50_Q18UU7 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Desulfitobacterium hafniense|Rep: Nitrilase/cyanide
hydratase and apolipoprotein N-acyltransferase -
Desulfitobacterium hafniense (strain DCB-2)
Length = 289
Score = 51.2 bits (117), Expect = 2e-05
Identities = 52/218 (23%), Positives = 93/218 (42%), Gaps = 2/218 (0%)
Frame = +3
Query: 63 VRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTRE 242
+R+GL Q + T++ L +++ + A+++ V++LC E A
Sbjct: 5 IRIGLAQFEAKVGD----TERNL---QEIIRTAEVASSQGVSLLCYPEC-----ALHGYS 52
Query: 243 KQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVI 422
+ + A+P L + L E A ++++ ++E+ + +V + +
Sbjct: 53 PKDASEIADP-LDSMAVARLRECARDLGLILLVGMVEKSPEGKKPYISQLIVFPDREPEV 111
Query: 423 GKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAE 602
+RK H+ R+ +YF P+F K +I IC+ H P + + GAE
Sbjct: 112 --YRKVHLGRI-----EQHYFTAGDSFPIFAAGGVKFSIGICWDWHFPELSAICSLKGAE 164
Query: 603 IVFNPSAT--VSGLSEHLWAVEARNAAIANSYYTCAIN 710
I F P A+ VSG + +W A NS Y CA N
Sbjct: 165 IQFAPHASPVVSGDRKEIWKRYLGARAYDNSVYLCACN 202
>UniRef50_A5D6C3 Cluster: Putative uncharacterized protein; n=1;
Pelotomaculum thermopropionicum SI|Rep: Putative
uncharacterized protein - Pelotomaculum
thermopropionicum SI
Length = 256
Score = 51.2 bits (117), Expect = 2e-05
Identities = 45/157 (28%), Positives = 67/157 (42%), Gaps = 14/157 (8%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPILERDD--IHGDTIWNTAVVINEFGKVIGKHRKNHIP 449
+ G T LAE A +Y + I LERD I +NT +I GK+I K+RK +
Sbjct: 86 IPGEETERLAEKAKEYQIYIAGCALERDKDWIDDGYFFNTHFIIGPDGKIIHKYRKITVA 145
Query: 450 RVGDFNESTY--YFEGNTGH--------PVFETKYGKVAINICYGRHHPLNWLMFGINGA 599
+ S + Y + H PV +T+ GK+ C H P G+ GA
Sbjct: 146 THYELAVSPHDVYDKFVAMHGDDLSVFLPVTDTEIGKIGTITCMDGHFPETARALGVQGA 205
Query: 600 EIVFNPSATVSGLS--EHLWAVEARNAAIANSYYTCA 704
E++ +P +S + +W + R A N Y A
Sbjct: 206 EVILHPLLVEPMMSPPQEIWQMMNRMRAWENVCYVIA 242
>UniRef50_Q5WM18 Cluster: Methylthioribose recycling protein; n=2;
Bacillaceae|Rep: Methylthioribose recycling protein -
Bacillus clausii (strain KSM-K16)
Length = 275
Score = 50.8 bits (116), Expect = 3e-05
Identities = 41/144 (28%), Positives = 68/144 (47%), Gaps = 1/144 (0%)
Frame = +3
Query: 282 GPSTV-FLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVG 458
G T+ FL +LA + + +++ + G I+NTA+VI+ GK++ + K H+ V
Sbjct: 74 GVETIAFLQQLARAHRIHMVAGSIATKKDGG--IYNTALVIDAQGKLVYTYDKVHL--VP 129
Query: 459 DFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGL 638
NE Y G+ +FE K+A+ ICY P + GAE++F +
Sbjct: 130 MLNEPAYMQGGSVPPALFELDGVKMAVLICYDLRFPELARRLALEGAEVLFIVAEWPLAR 189
Query: 639 SEHLWAVEARNAAIANSYYTCAIN 710
+ H W + AI N +Y + N
Sbjct: 190 AMH-WKALQQARAIENQFYLLSCN 212
>UniRef50_Q2LUZ0 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Syntrophus aciditrophicus SB|Rep: Carbon-nitrogen
hydrolase family protein - Syntrophus aciditrophicus
(strain SB)
Length = 268
Score = 50.8 bits (116), Expect = 3e-05
Identities = 37/159 (23%), Positives = 78/159 (49%), Gaps = 1/159 (0%)
Frame = +3
Query: 135 IFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELA 314
+ E +Q+ S + ++L L E +N + F ++ + AE + G +T FL +A
Sbjct: 15 VAENLQQTESLINCTKADLLVLPELFNTGYLFTAHQEV--AELAEEIPGGRTTEFLCGMA 72
Query: 315 VKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFE-G 491
+ I++ + ER+ +N+AV+++ G +G +RK H+ FNE +F+ G
Sbjct: 73 RRGGSFIVAGLAEREK---GRFYNSAVLVSPRG-YLGTYRKIHL-----FNEEKLWFQPG 123
Query: 492 NTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIV 608
+ +++ ++ I IC+ P + + GA+++
Sbjct: 124 DRAPELYDLGICRIGIMICFDWFFPEFMRILSLKGADVI 162
>UniRef50_Q1F028 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Clostridium
oremlandii OhILAs|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Clostridium
oremlandii OhILAs
Length = 261
Score = 50.8 bits (116), Expect = 3e-05
Identities = 50/192 (26%), Positives = 84/192 (43%), Gaps = 1/192 (0%)
Frame = +3
Query: 138 FEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFL-AELA 314
F+K +++I AA E + + L E W+ F F + +CD G T L ++L+
Sbjct: 19 FKKAEELIRLAAKENPDTIALPETWSTGF-FPKENIKEFCD-----QNGNRTKRLFSKLS 72
Query: 315 VKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGN 494
+ ++ II+ + + D I+NT+ + N+ G+ I ++ K H+ + YFE
Sbjct: 73 KELNVNIIAGSVINE--KQDGIYNTSYIFNKQGECIAEYDKTHL---FSYMGEDQYFEKG 127
Query: 495 TGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNA 674
+G VFE K I ICY + +I+F A L H W +
Sbjct: 128 SGITVFELDGIKCGIVICYDIRFVELVRTLALQEIKILF-VVAQWPMLRIHHWQILNEAR 186
Query: 675 AIANSYYTCAIN 710
AI N + +N
Sbjct: 187 AIENQIFVACVN 198
>UniRef50_Q47VH0 Cluster: Hydrolase, carbon-nitrogen family; n=1;
Colwellia psychrerythraea 34H|Rep: Hydrolase,
carbon-nitrogen family - Colwellia psychrerythraea
(strain 34H / ATCC BAA-681) (Vibriopsychroerythus)
Length = 273
Score = 50.4 bits (115), Expect = 4e-05
Identities = 43/193 (22%), Positives = 82/193 (42%), Gaps = 6/193 (3%)
Frame = +3
Query: 144 KVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVF-LAELAVK 320
K+ +++S A Q ++ L F +++ + D A TG L ELA K
Sbjct: 21 KIAELLSKITASQEDVQHLVVLPECCLYFGSKDSEQ-LDLAIASATGNDLCLALGELAKK 79
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI--PRVGDFNES---TYYF 485
+ + +++ + N++ V N G++IG++ K H+ V D +S + Y
Sbjct: 80 FKVYLVAGTIPILSTSSTKFTNSSCVFNPEGELIGQYDKIHLFDVNVSDSTKSYCESRYT 139
Query: 486 EGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEA 665
+ + T++ + +++C+ P + I GA+I+ PSA + W
Sbjct: 140 QAGKEISMVNTEFANIGLSVCFDLRFPNLFQQLSIAGADIITVPSAFTRVTGKAHWQTLL 199
Query: 666 RNAAIANSYYTCA 704
+ AI N Y A
Sbjct: 200 QARAIENQVYIVA 212
>UniRef50_A4EPU1 Cluster: Putative hydrolase; n=2;
Rhodobacteraceae|Rep: Putative hydrolase - Roseobacter
sp. SK209-2-6
Length = 264
Score = 50.4 bits (115), Expect = 4e-05
Identities = 56/192 (29%), Positives = 83/192 (43%), Gaps = 4/192 (2%)
Frame = +3
Query: 147 VQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYD 326
+++ +S + V++L L E + + +R D AEP GPS +AELA +
Sbjct: 26 LRECLSQLDGQHVDLLLLPELFLTGYNIGSRVT----DRAEPA-DGPSAQAIAELARAHR 80
Query: 327 MVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP 506
+ I ER D I+N+A I++ G ++ HRK +P F E ++ G G+
Sbjct: 81 IAIHYGFAERQD---GQIFNSASCISKDGTLLATHRKLLLPP--GF-EGDHFCPG-IGYT 133
Query: 507 VFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNA---- 674
FE VA ICY P + GAE+V P+A + W V A
Sbjct: 134 QFELNGFNVATLICYDAEFPETFRAVAQAGAELVLVPTALGA-----QWGVVANTVIPAR 188
Query: 675 AIANSYYTCAIN 710
A N Y C N
Sbjct: 189 AFENGIYVCYAN 200
>UniRef50_P58054 Cluster: UPF0012 hydrolase ybeM; n=33;
Proteobacteria|Rep: UPF0012 hydrolase ybeM - Escherichia
coli O157:H7
Length = 262
Score = 50.4 bits (115), Expect = 4e-05
Identities = 33/109 (30%), Positives = 49/109 (44%), Gaps = 1/109 (0%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRH 560
WN V + + G ++ ++ K H+ ES GN P+ E + KV + CY
Sbjct: 93 WNMLVAL-QAGNIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLR 151
Query: 561 HPLNWLMFGINGAEIVFNPSATVSG-LSEHLWAVEARNAAIANSYYTCA 704
P L + GAEI+ P+A V G L EH W+ A+ + Y A
Sbjct: 152 FPELALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVA 200
>UniRef50_Q86X76 Cluster: Nitrilase homolog 1; n=29; Eumetazoa|Rep:
Nitrilase homolog 1 - Homo sapiens (Human)
Length = 327
Score = 50.4 bits (115), Expect = 4e-05
Identities = 37/114 (32%), Positives = 47/114 (41%), Gaps = 5/114 (4%)
Frame = +3
Query: 378 IWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFEGNTGHPVFETKYGKVAIN 542
I+N V++N G V+ +RK H IP G ES G + T GK+ +
Sbjct: 142 IYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLA 201
Query: 543 ICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
+CY P L GAEI+ PSA S W V R AI Y A
Sbjct: 202 VCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVA 255
>UniRef50_Q84FR7 Cluster: D-N-carbamoylase; n=1; Arthrobacter
crystallopoietes|Rep: D-N-carbamoylase - Arthrobacter
crystallopoietes
Length = 315
Score = 50.0 bits (114), Expect = 6e-05
Identities = 26/84 (30%), Positives = 45/84 (53%), Gaps = 8/84 (9%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRVGDFN--------ESTYYFEGNTGHPVFETKYGKVA 536
+NT++++N+ G ++GK+RK H+P D E Y+ EG+ G VF+ +V
Sbjct: 105 YNTSILVNKHGDIVGKYRKMHLPGHADNREGLPNQHLEKKYFREGDLGFGVFDFHGVQVG 164
Query: 537 INICYGRHHPLNWLMFGINGAEIV 608
+ +C R P + + GAE+V
Sbjct: 165 MCLCNDRRWPEVYRSLALQGAELV 188
>UniRef50_Q6JHR5 Cluster: Aliphatic amidase; n=1; Saccharopolyspora
spinosa|Rep: Aliphatic amidase - Saccharopolyspora
spinosa
Length = 308
Score = 49.6 bits (113), Expect = 8e-05
Identities = 27/83 (32%), Positives = 46/83 (55%), Gaps = 1/83 (1%)
Frame = +3
Query: 378 IWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP-VFETKYGKVAINICYG 554
++NTA+ + G +G +RK HIP +G + G+ G P VF+T +G+V + IC+
Sbjct: 113 VYNTALALGPAG-TLGHYRKQHIPFMG---ADRFVAPGDDGAPRVFDTPFGRVGMMICFD 168
Query: 555 RHHPLNWLMFGINGAEIVFNPSA 623
P + + GA+I+ P+A
Sbjct: 169 LRFPESARELALAGADIIVMPTA 191
>UniRef50_A3CTE8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Methanoculleus
marisnigri JR1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Methanoculleus
marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Length = 265
Score = 49.6 bits (113), Expect = 8e-05
Identities = 49/190 (25%), Positives = 76/190 (40%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
E ++ AAA +++C E F K P EP L GP T A +A +
Sbjct: 21 EAAGRMAGEAAAAGASLICFPEQ----FVTGWSPKVP-PGSGEP-LDGPLTAAFARIAEE 74
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
+ + I+E + NT VV++E G+++ + K H+ E YY G+
Sbjct: 75 NGIAVAGSIVEAGLENRPK--NTTVVLDEDGELLAAYAKIHL--FSPEGEDRYYTAGDR- 129
Query: 501 HPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAI 680
F K I +CY P + ++ I G E + P+A H W A+
Sbjct: 130 IATFTVDGVKFGIAVCYDLRFPELFRIYAIAGVECMLVPAAWPCSRLSH-WETLLPARAL 188
Query: 681 ANSYYTCAIN 710
N YY +N
Sbjct: 189 ENRYYVTGVN 198
>UniRef50_A3EVA0 Cluster: NAD synthase; n=4; Bacteria|Rep: NAD
synthase - Leptospirillum sp. Group II UBA
Length = 592
Score = 49.2 bits (112), Expect = 1e-04
Identities = 30/87 (34%), Positives = 48/87 (55%), Gaps = 2/87 (2%)
Frame = +3
Query: 372 DTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICY 551
D I+N A V++ GK+ G +RK ++P G F+E+ Y+ EG PV E + ++ INIC
Sbjct: 90 DDIYNAAAVLHG-GKLHGIYRKQYLPNYGVFDENRYFQEG-VESPVLEYRSARLGINICE 147
Query: 552 GRHHPLN--WLMFGINGAEIVFNPSAT 626
+P + + AE + N SA+
Sbjct: 148 DIWYPKGPLYTQTLMGDAECILNLSAS 174
>UniRef50_Q9HIW8 Cluster: Nitrilase related protein; n=2;
Thermoplasma|Rep: Nitrilase related protein -
Thermoplasma acidophilum
Length = 270
Score = 49.2 bits (112), Expect = 1e-04
Identities = 37/145 (25%), Positives = 68/145 (46%), Gaps = 1/145 (0%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
+EP L G + E+A II I ER+ + +NTA+ I+E G ++ K+RK H
Sbjct: 56 SEP-LDGKFVKSITEIARSESQKIILNIPERNQYNLKP-FNTAIYIDELGLIL-KYRKLH 112
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+ F ES+ + +G+ +F + + ICY P M ++GA+++ +
Sbjct: 113 LFDAFGFRESSVFEKGDARPAIFNGSGDPLGVLICYDLRFPEPARMLALDGAKLIIYQAG 172
Query: 624 TVSGLSEH-LWAVEARNAAIANSYY 695
+G ++ W + A+ N +
Sbjct: 173 WFAGERKYDQWKTLLKARAMENGVF 197
>UniRef50_Q3W243 Cluster: GCN5-related N-acetyltransferase:AIR
synthase related protein:Nitrilase/cyanide hydratase and
apolipoprotein N- acyltransferase:AIR synthase related
protein, C-terminal; n=14; Actinomycetales|Rep:
GCN5-related N-acetyltransferase:AIR synthase related
protein:Nitrilase/cyanide hydratase and apolipoprotein
N- acyltransferase:AIR synthase related protein,
C-terminal - Frankia sp. EAN1pec
Length = 807
Score = 48.8 bits (111), Expect = 1e-04
Identities = 36/109 (33%), Positives = 55/109 (50%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
GP LA A+ DMV+ + ERD G +N+AV ++ G V+G+HRK H P
Sbjct: 563 GPEITRLA--AIAGDMVVCAGYAERD---GRYRYNSAVCVHGDG-VLGRHRKVHQP---- 612
Query: 462 FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIV 608
ES Y E F++ G++ + ICY + P + + GA+I+
Sbjct: 613 LGESLAY-EAGRSFTAFDSPLGRMGMMICYDKAFPESGRSLALAGADII 660
>UniRef50_A5TTZ3 Cluster: Possible amidohydrolase; n=1;
Fusobacterium nucleatum subsp. polymorphum ATCC
10953|Rep: Possible amidohydrolase - Fusobacterium
nucleatum subsp. polymorphum ATCC 10953
Length = 274
Score = 48.8 bits (111), Expect = 1e-04
Identities = 50/192 (26%), Positives = 87/192 (45%), Gaps = 2/192 (1%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWC-DFAEPVLTGPSTVFLAELAV 317
+K+ + I AA E V+I+C E + + T E Q DF + L E A
Sbjct: 28 KKIFERIEEAAKENVDIICFPELATIGYTITTDELQNLPEDFNNTFIEK-----LQEKAK 82
Query: 318 KYDMVIISPILE-RDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGN 494
+ + I+ LE + +N+ + I++ GK++ RK ++ + E T + G+
Sbjct: 83 LFKIHILVGYLESKTTKKSKDFYNSCIFIDDEGKILANARKVYLWK----KEKTKFKAGD 138
Query: 495 TGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNA 674
V +TK+GK+ I ICY + + GAEI+F PS S +E+ W ++
Sbjct: 139 K-FIVKDTKFGKIGILICYDLEFFEPARIECLKGAEIIFVPSLW-SLNAENRWHIDLAAN 196
Query: 675 AIANSYYTCAIN 710
++ N + N
Sbjct: 197 SLFNLLFMVGCN 208
>UniRef50_A1S062 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Thermofilum
pendens Hrk 5|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Thermofilum pendens
(strain Hrk 5)
Length = 279
Score = 48.8 bits (111), Expect = 1e-04
Identities = 38/121 (31%), Positives = 55/121 (45%), Gaps = 9/121 (7%)
Frame = +3
Query: 378 IWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP-VFETKYGKVAINICYG 554
I+ +V + G V G K ++P G F ES Y+ EG+ VFE +VA IC
Sbjct: 92 IYENSVAVVRDGSVAGVVSKLYLPDYGLFEESRYFREGSCSREGVFECGGWRVAPIICED 151
Query: 555 RHHPLNWLMFGINGAEIVF-NPSATVSGL-------SEHLWAVEARNAAIANSYYTCAIN 710
HP + GA++VF + S+ + GL E +W A A+ N+ Y N
Sbjct: 152 AWHPEPAELAARRGADVVFIHASSPIRGLYGSGEANIERVWEAIAVTRAVENACYVVFAN 211
Query: 711 R 713
R
Sbjct: 212 R 212
>UniRef50_Q1LPP8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=5;
Betaproteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Ralstonia
metallidurans (strain CH34 / ATCC 43123 / DSM 2839)
Length = 273
Score = 48.4 bits (110), Expect = 2e-04
Identities = 37/147 (25%), Positives = 66/147 (44%), Gaps = 6/147 (4%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI----P 449
GP FLA+ A ++ + ++ L ++NT++ + G+ + ++ K H+
Sbjct: 68 GPVQSFLADAARRHRVWLVGGTLPMWCNDDARVYNTSLAFDPHGRRVARYDKIHLFGFTK 127
Query: 450 RVGDFNESTYYFEGNTGHPV-FETKYGKVAINICYGRHHP-LNWLMFGINGAEIVFNPSA 623
++ES G T PV F+ G+VA+++CY P L + G N ++ P+A
Sbjct: 128 GTESYDESRTILAGKT--PVAFDAPCGRVAMSVCYDLRFPELYRGLAGKNDVSLILMPAA 185
Query: 624 TVSGLSEHLWAVEARNAAIANSYYTCA 704
+ W + R AI N Y A
Sbjct: 186 FTYTTGQAHWEILLRARAIENQCYVLA 212
>UniRef50_Q2QQ94 Cluster: Hydrolase, carbon-nitrogen family protein,
expressed; n=4; Magnoliophyta|Rep: Hydrolase,
carbon-nitrogen family protein, expressed - Oryza sativa
subsp. japonica (Rice)
Length = 323
Score = 48.4 bits (110), Expect = 2e-04
Identities = 52/200 (26%), Positives = 86/200 (43%), Gaps = 9/200 (4%)
Frame = +3
Query: 132 AIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAEL 311
A + ++ AA+ V LC E F+F + AEP L GP L
Sbjct: 61 ANYATCSRLAKEAASSGVKFLCFPEV----FSFIGSKDGESIKIAEP-LDGPIMQRYCSL 115
Query: 312 AVKYDMVI-ISPILER--DDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFN 467
A + M + + E+ DD H +NT V+I++ G++ +RK H +P +
Sbjct: 116 AKESSMWLSLGGFQEKGPDDSHQ---YNTHVLIDDSGEIRSSYRKIHLFDVDVPGNMVYK 172
Query: 468 ESTYYFEGNTGHPVFETKYGKVAINICYGRHHP-LNWLMFGINGAEIVFNPSATVSGLSE 644
ES + G+T V ++ +G++ + +CY P L + + A+++ PSA E
Sbjct: 173 ESRFTTAGDTVVAV-DSPFGRLGLTVCYDLRFPELYQCLRFKHQAQVLLVPSAFTKVTGE 231
Query: 645 HLWAVEARNAAIANSYYTCA 704
W + R AI Y A
Sbjct: 232 AHWEILLRARAIETQCYVIA 251
>UniRef50_P55175 Cluster: UPF0012 hydrolase sll0601; n=40;
Cyanobacteria|Rep: UPF0012 hydrolase sll0601 -
Synechocystis sp. (strain PCC 6803)
Length = 272
Score = 48.4 bits (110), Expect = 2e-04
Identities = 45/196 (22%), Positives = 87/196 (44%), Gaps = 8/196 (4%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
++ +++I A + ++ L E FAF E + + A + T FL +A +
Sbjct: 22 QEAEELIDLAVRQGAELVGLPEN----FAFLGNETEK-LEQATAIATATEK-FLQTMAQR 75
Query: 321 YDMVIISPILERDDIHGDT--IWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTY 479
+ + I++ + G+ +NTA +I G+ + ++ K H +P + ES
Sbjct: 76 FQVTILAGGFPFP-VAGEAGKAYNTATLIAPNGQELARYHKVHLFDVNVPDGNTYWESAT 134
Query: 480 YFEGNTGHPVFET-KYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWA 656
G PV+ + +G + ++ICY P + GA+++F P+A + + W
Sbjct: 135 VMAGQKYPPVYHSDSFGNLGLSICYDVRFPELYRYLSRQGADVLFVPAAFTAYTGKDHWQ 194
Query: 657 VEARNAAIANSYYTCA 704
V + AI N+ Y A
Sbjct: 195 VLLQARAIENTCYVIA 210
>UniRef50_Q6RWQ0 Cluster: Nitrilase; n=3; uncultured organism|Rep:
Nitrilase - uncultured organism
Length = 325
Score = 48.0 bits (109), Expect = 2e-04
Identities = 61/229 (26%), Positives = 98/229 (42%), Gaps = 19/229 (8%)
Frame = +3
Query: 78 IQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEA----------WNMPFA 227
I + AI + PI A EK ++I AA N++ EA W A
Sbjct: 7 IVRAAAIQAE-PIVLDCDATVEKACRLIGEAAENGANLIVFPEAFIPVYPNAAIWGRGLA 65
Query: 228 FC--TREKQPWCDF--AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAV 395
R+K W + GP+T LA+ A + ++ + ER + +T++NT +
Sbjct: 66 TFGGQRQKYVWTRLWNNSVEIPGPATDRLAKAAHEARATVVMGLNERA-VDNNTLYNTLL 124
Query: 396 VINEFGKVIGKHRK----NHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHH 563
I G+++GKHRK NH + +G+T VF+T GKV IC+ +
Sbjct: 125 FIGPDGRLLGKHRKLMPTNHERMIWGMG------DGSTLR-VFDTPCGKVGGLICWENYM 177
Query: 564 PL-NWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAI 707
PL + ++G G +I P+A + V ARN A + ++
Sbjct: 178 PLARYALYG-QGEQIHVAPTAH----DGEITLVNARNTAYEGRLFVISV 221
>UniRef50_A0JSY8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=4;
Actinomycetales|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Arthrobacter sp.
(strain FB24)
Length = 344
Score = 48.0 bits (109), Expect = 2e-04
Identities = 41/168 (24%), Positives = 75/168 (44%), Gaps = 16/168 (9%)
Frame = +3
Query: 258 DFAEPVLTGPSTVFLAELAVKYDMVIISPILER---DDIHGDTIW-NTAVVINEFGKVIG 425
D AE +LTGP+ F A A ++ + + + + +R D D + NT+V+++ G+++
Sbjct: 91 DLAEDLLTGPTFRFAAGAARRHGITVHASLYQRAENPDGSDDGLGLNTSVLVSPEGELLA 150
Query: 426 KHRKNHIPRVGDFNESTYYFEGNTGHPVFETKY------GKVAINICYGRHHPLNWLMFG 587
+ K HIP + E ++ G +E ++ + C+ P ++
Sbjct: 151 RTHKLHIPVTAGYYEDKFFRPGPAVEDAYEVHSPAELGGARLGMPTCWDEWFPELARLYS 210
Query: 588 INGAEIVFNPSATVSGL------SEHLWAVEARNAAIANSYYTCAINR 713
+ GAEI+ P+A S ++ LW IAN + A NR
Sbjct: 211 LGGAEILVYPTAIGSEPDHPDFDTQPLWQQVIVGNGIANGLFMVAPNR 258
>UniRef50_A0BR54 Cluster: Chromosome undetermined scaffold_122,
whole genome shotgun sequence; n=2;
Oligohymenophorea|Rep: Chromosome undetermined
scaffold_122, whole genome shotgun sequence - Paramecium
tetraurelia
Length = 281
Score = 48.0 bits (109), Expect = 2e-04
Identities = 41/159 (25%), Positives = 71/159 (44%), Gaps = 6/159 (3%)
Frame = +3
Query: 108 NPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGP 287
N IT + V+ I AA + + L E +N + + DF + TG
Sbjct: 12 NAITATKTQTLALVKDQIKEAAIQGSKVCILGECFNSYYVKAQLQNNAE-DFGK---TGE 67
Query: 288 STVF--LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
++E++ ++ ++II I E+ GD ++NTA N G+++ +RK H+ +
Sbjct: 68 RQTLDLISEISKQFGIMIIGSIPEKS---GDKMYNTAFCFNN-GQLLVTYRKTHLFDIDI 123
Query: 462 FNESTYY----FEGNTGHPVFETKYGKVAINICYGRHHP 566
+ TY F + + +T+YGK I ICY P
Sbjct: 124 PGKITYKESLTFSAGDNYKIVDTEYGKFGIGICYDIRFP 162
>UniRef50_Q3A0A3 Cluster: Predicted amidohydrolase; n=1; Pelobacter
carbinolicus DSM 2380|Rep: Predicted amidohydrolase -
Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1)
Length = 278
Score = 47.6 bits (108), Expect = 3e-04
Identities = 51/197 (25%), Positives = 84/197 (42%), Gaps = 7/197 (3%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
E + + AA + ++C E A C + + AE V+ G ++ LA LA K
Sbjct: 31 EGIARWTEQAARQGAEMVCFPE-----LAICGYTRSGIGELAE-VVPGRASCHLAALARK 84
Query: 321 YDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTG 500
+ MV+ + ++E+ G + T +V + G I ++RK H+ R E + G+
Sbjct: 85 HRMVVSAGLIEKS---GSACYITQLVASADGS-IERYRKTHLGR----REREVFCAGDA- 135
Query: 501 HPVFETKYGK-----VAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSE--HLWAV 659
PVF T+ AI +CY H P + + GA+++ P A + LW
Sbjct: 136 LPVFTTRSRAGMPITFAIGLCYDLHFPELATAYAVQGAQLLLAPHAAPHAGPDRMQLWQR 195
Query: 660 EARNAAIANSYYTCAIN 710
A N+ Y A N
Sbjct: 196 YMGARAYDNTMYVAACN 212
>UniRef50_UPI0000D55F17 Cluster: PREDICTED: similar to CG7067-PA;
n=2; Coelomata|Rep: PREDICTED: similar to CG7067-PA -
Tribolium castaneum
Length = 445
Score = 46.8 bits (106), Expect = 5e-04
Identities = 54/192 (28%), Positives = 82/192 (42%), Gaps = 6/192 (3%)
Frame = +3
Query: 147 VQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYD 326
V++++S AA +Q I+ L EA + + K FAEP L G LA K
Sbjct: 26 VKQLVSEAAQKQAKIVFLPEASD----YIAANKNEAKAFAEP-LNGTLMNEYRNLA-KTR 79
Query: 327 MVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVG-DFNESTYYFE 488
V +S + ++ I+NT V+I++ G++ ++K H IP + + ES
Sbjct: 80 KVWLSVGGFHELVNEHQIFNTHVLIDDEGEIKSVYKKLHLFDVSIPELNVNLRESDLNEA 139
Query: 489 GNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEAR 668
G P T G +A+ ICY P ++ GA I+ PSA W R
Sbjct: 140 GRHLVPPVMTPAGPLALAICYDLRFPELSIIQRKQGANILTYPSAFTKATGALHWETLLR 199
Query: 669 NAAIANSYYTCA 704
+ AI Y A
Sbjct: 200 SRAIETQCYVIA 211
>UniRef50_Q0HEI5 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=18; Shewanella|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Shewanella sp. (strain MR-4)
Length = 282
Score = 46.8 bits (106), Expect = 5e-04
Identities = 35/140 (25%), Positives = 63/140 (45%), Gaps = 5/140 (3%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
L+ LA +Y + +++ + G +++ + ++ G +G++ K H+ V + +
Sbjct: 75 LSALAARYCVYMVAGTIPALAEDG-RVYSRCYLFDDKGDTLGQYDKLHLFDVDVADGTKQ 133
Query: 480 YFEGNTGHP-----VFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSE 644
Y E T P V +T +GK+ + ICY P + + GAEI+ PSA E
Sbjct: 134 YRESETFCPGNHISVIDTPFGKIGLTICYDLRFPDLFRALRLAGAEIITVPSAFTKVTGE 193
Query: 645 HLWAVEARNAAIANSYYTCA 704
W V + AI + A
Sbjct: 194 AHWQVLLQARAIETQCFILA 213
>UniRef50_A5WCY0 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=42; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Psychrobacter sp. PRwf-1
Length = 545
Score = 46.8 bits (106), Expect = 5e-04
Identities = 48/193 (24%), Positives = 82/193 (42%), Gaps = 6/193 (3%)
Frame = +3
Query: 135 IFEKVQKIISAAAAEQVNILCLQEAWNMPF-AFCTREKQPWCDFAEPVLTGPSTVF---L 302
+ ++V+ + A + CL E +N P C Q + A L + F +
Sbjct: 242 LLQQVEFFVDIMADYNADFACLPEFFNAPLMGLCESTDQ---NIAIRFLADYTEWFKNEI 298
Query: 303 AELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYY 482
+ LAV Y++ +I+ + D + + ++N + + G V + RK HI S +
Sbjct: 299 SNLAVSYNVNVITGSMPLFDENEEVLYNVSYLCRRDGTV-EEQRKIHIT---PHERSAWV 354
Query: 483 FEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNP--SATVSGLSEHLWA 656
EG VF+T G++ I ICY P + + +I+F P + T +G
Sbjct: 355 IEGGNKVQVFDTDAGRIGILICYDVEFPELARLLALEDMDILFVPFWTDTKNGYLRVRHC 414
Query: 657 VEARNAAIANSYY 695
+AR AI N Y
Sbjct: 415 AQAR--AIENECY 425
>UniRef50_A4XN12 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1;
Caldicellulosiruptor saccharolyticus DSM 8903|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Caldicellulosiruptor saccharolyticus
(strain ATCC 43494 / DSM 8903)
Length = 287
Score = 46.8 bits (106), Expect = 5e-04
Identities = 45/166 (27%), Positives = 78/166 (46%)
Frame = +3
Query: 123 QRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFL 302
+R ++E + +I AA + +++ EA N R K + +P L G + +
Sbjct: 31 KRKTLYE-ICYLIEQAAKDHPDLIVTPEAVNAIIPSNKRTKF-FKQLTDP-LDGETVKKV 87
Query: 303 AELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYY 482
E+A KY I+ + + + +N+A+ IN G ++ + K H+ VG E T
Sbjct: 88 CEIAKKYRCNIVVGLYTSRE---NKAYNSALFINRKGDIVDVYDKVHLA-VG---EETNL 140
Query: 483 FEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPS 620
GN VF+T GKV I IC+ P + ++GA+I+ P+
Sbjct: 141 CPGNE-FKVFDTDIGKVGILICWDMQFPEAARILALSGADIIICPT 185
>UniRef50_A0U0W3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=4; Burkholderia
cepacia complex|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Burkholderia
cenocepacia MC0-3
Length = 275
Score = 46.8 bits (106), Expect = 5e-04
Identities = 39/151 (25%), Positives = 66/151 (43%), Gaps = 1/151 (0%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
AEP L GPS + A + ++ + E+DD +NTA++++EFG++ ++RK+H
Sbjct: 59 AEP-LDGPSVSAIRAAARDAHVAVVIGVAEQDD---GRYFNTAILVDEFGELRLRYRKSH 114
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+ + FE V E + KV + IC+ P GAE++ P
Sbjct: 115 L-----YESDVGVFEAGGTFDVCEWRGVKVGMLICFDLEFPETARALARAGAELIVIPDG 169
Query: 624 TVSGLSE-HLWAVEARNAAIANSYYTCAINR 713
+ H + R A+ N + NR
Sbjct: 170 MMQPHGHVHRKMIPVR--ALENQVFVAMANR 198
>UniRef50_Q3IW15 Cluster: Predicted amidohydrolase; n=2; Rhodobacter
sphaeroides|Rep: Predicted amidohydrolase - Rhodobacter
sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 /
DSM158)
Length = 280
Score = 46.4 bits (105), Expect = 7e-04
Identities = 45/137 (32%), Positives = 61/137 (44%), Gaps = 16/137 (11%)
Frame = +3
Query: 261 FAEPVLTGPS---TVFLAELAVKYDMVIISPIL---ERDDIH---------GDTIWNTAV 395
F E LTG S L + AV + ++PIL DIH G NTA
Sbjct: 39 FPECFLTGGSFDDRAALLQAAVDIERGDLAPILLAAREADIHVVVGFYQKSGPQALNTAA 98
Query: 396 VINEFGKVIGKHRKNHIP-RVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLN 572
+I G +IG H K H+P +GD EG + VF+T G++ + ICY P
Sbjct: 99 LIGPEG-IIGLHHKMHLPFMIGDRFADIPQIEGPS---VFDTAIGRIGLAICYEIRFPEV 154
Query: 573 WLMFGINGAEIVFNPSA 623
+ GAE+V P+A
Sbjct: 155 IRTLALEGAELVVLPAA 171
>UniRef50_Q75TH8 Cluster: Putative uncharacterized protein GSB07;
n=1; Geobacillus stearothermophilus|Rep: Putative
uncharacterized protein GSB07 - Bacillus
stearothermophilus (Geobacillus stearothermophilus)
Length = 273
Score = 46.4 bits (105), Expect = 7e-04
Identities = 36/145 (24%), Positives = 69/145 (47%), Gaps = 1/145 (0%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
G + +++LA + + + +E+D H ++N+ ++I+ G+ IG +RK H+
Sbjct: 68 GSTFQHMSQLAQTFQLYLAYGYVEKD--HTGNLYNSLMLIDPNGQCIGNYRKIHLTPF-- 123
Query: 462 FNESTYYFEGNTGHPVF-ETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGL 638
E ++ +G PV +T+ G++ + IC+ P ++GAE++ P A S
Sbjct: 124 --EKAWFSKG--AEPVLVDTELGRIGLMICWDLAFPELARYLAVHGAELLLVPCAWESPF 179
Query: 639 SEHLWAVEARNAAIANSYYTCAINR 713
A AI N+ Y A N+
Sbjct: 180 HAPFQKF-AMARAIDNTVYVAACNQ 203
>UniRef50_A1SU00 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Psychromonas
ingrahamii 37|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Psychromonas
ingrahamii (strain 37)
Length = 274
Score = 46.4 bits (105), Expect = 7e-04
Identities = 43/172 (25%), Positives = 66/172 (38%), Gaps = 9/172 (5%)
Frame = +3
Query: 225 AFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVIN 404
A C +K + +E + G L+ LA Y +I D I+ T +V +
Sbjct: 44 ALCIADKDHYLALSENLGKGYYQSLLSALAKHYQCYLICGSFPIKSTITDKIFTTCLVFS 103
Query: 405 EFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETK--------YG-KVAINICYGR 557
G++I + K H+ + Y E +T P E K Y KV + ICY
Sbjct: 104 PLGELISHYHKMHLFDAQVADHKGIYKESDTFVPGQEVKLFNWDCGAYSVKVGLTICYDL 163
Query: 558 HHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
P + GA+I+ P+A + W + AI N Y A N+
Sbjct: 164 RFPGLFQTLRKQGADILLVPAAFTQTTGQAHWLPLLQARAIENQCYIIAANQ 215
>UniRef50_A0R400 Cluster: Hydrolase, carbon-nitrogen family protein;
n=1; Mycobacterium smegmatis str. MC2 155|Rep:
Hydrolase, carbon-nitrogen family protein -
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Length = 281
Score = 46.4 bits (105), Expect = 7e-04
Identities = 26/86 (30%), Positives = 44/86 (51%)
Frame = +3
Query: 360 DIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAI 539
++ GDT++N+A+VI + GKV+G +RK H+ E + G V +T ++ +
Sbjct: 90 EVDGDTLYNSAIVIGD-GKVVGTYRKAHLWAA----EPEIFATGVEAGTVIDTAICRLGV 144
Query: 540 NICYGRHHPLNWLMFGINGAEIVFNP 617
ICY P + GAE++ P
Sbjct: 145 AICYDNEFPELPRRLALRGAEVLALP 170
>UniRef50_A0LH50 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Syntrophobacter
fumaroxidans MPOB|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Syntrophobacter
fumaroxidans (strain DSM 10017 / MPOB)
Length = 260
Score = 46.4 bits (105), Expect = 7e-04
Identities = 35/133 (26%), Positives = 63/133 (47%)
Frame = +3
Query: 312 AVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEG 491
A ++ MV++ + E D I+NT+ VI+ G++ G +RK H+ + +E ++ G
Sbjct: 73 ARRHGMVLVGSLPESVD---GRIYNTSYVIDANGEIAGSYRKVHLFSL--HHEDLHFGRG 127
Query: 492 NTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARN 671
T V T+ G++ + ICY P ++GA I+ S +H W++ R
Sbjct: 128 ETS-LVCSTEAGELGVMICYDLRFPELGRKLALDGARIMCVSSHWPDIRIDH-WSLLLRA 185
Query: 672 AAIANSYYTCAIN 710
A+ N + N
Sbjct: 186 RAVENQLFVIGCN 198
>UniRef50_Q8FM85 Cluster: Putative uncharacterized protein; n=2;
Corynebacterium|Rep: Putative uncharacterized protein -
Corynebacterium efficiens
Length = 296
Score = 46.0 bits (104), Expect = 0.001
Identities = 49/176 (27%), Positives = 77/176 (43%), Gaps = 6/176 (3%)
Frame = +3
Query: 126 RLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLA 305
++A E V+ + AAA+ +L EA + F ++Q AE + TG +
Sbjct: 47 KMANLELVRTTATDAAAQGARLLIYPEATSQAFGTGRLDEQ-----AEDLHTGAFATGVQ 101
Query: 306 ELAVKYDMVIISPI------LERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFN 467
+LA +VI++ + +E+D + NTA+V G G H+ N G +
Sbjct: 102 QLAEDLGVVIVAGMFTPADTVEQDGKTLHRVHNTALVTGN-GLHEGYHKINTYDAFG-YR 159
Query: 468 ESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSG 635
ES GN H VF+ KV + ICY P + GAEI+ P++ G
Sbjct: 160 ESDTVKPGNELH-VFDLDGVKVGVAICYDLRFPTQFQELARAGAEIIVVPTSWQDG 214
>UniRef50_A4BGL8 Cluster: Predicted amidohydrolase; n=1; Reinekea
sp. MED297|Rep: Predicted amidohydrolase - Reinekea sp.
MED297
Length = 271
Score = 46.0 bits (104), Expect = 0.001
Identities = 31/112 (27%), Positives = 52/112 (46%), Gaps = 6/112 (5%)
Frame = +3
Query: 396 VINEFGKVIGKHRKNHI------PRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGR 557
V++ G+++G + K H+ R G + ES Y G+ + T +G++ +++CY
Sbjct: 104 VVDADGELVGFYDKIHLFDAEVGDRQGAYRESDSYSGGDKVVTLL-TPWGRLGLSVCYDL 162
Query: 558 HHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
P + GA+ V PSA + E W R AI N Y A+N+
Sbjct: 163 RFPELFRALNDQGADFVTLPSAFTAKTGEAHWEPLCRARAIENGYSLIAVNQ 214
>UniRef50_Q5DC61 Cluster: SJCHGC06938 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC06938 protein - Schistosoma
japonicum (Blood fluke)
Length = 290
Score = 46.0 bits (104), Expect = 0.001
Identities = 56/227 (24%), Positives = 94/227 (41%), Gaps = 15/227 (6%)
Frame = +3
Query: 60 IVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQ-VNILCLQEAWNMPFAFCT 236
++RL L+Q + + TD + A ++ +IS A +E ++CL E + P
Sbjct: 1 MLRLALVQ--MFVGTD------KAANLKRASDLISRAVSEHSAQLVCLPECFTSPIG--A 50
Query: 237 REKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGK 416
+ +P +AEPV GP+ L+ A + + ++ + G I+N N G+
Sbjct: 51 KYFEP---YAEPVPNGPACQMLSNAAKSHKIWLVGGSISERGSDGK-IYNCCATYNPDGE 106
Query: 417 VIGKHRKNH-----IPRVGDFNES--------TYYFEGNTGHPVFETKYGKVAINICYGR 557
++G +RK H IP F ES T+ FE + +V I ICY
Sbjct: 107 LVGLYRKLHLFDIDIPGQFTFKESASLSSGKETFSFEMPLKSSENKISVIRVGIGICYDI 166
Query: 558 HHP-LNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYY 695
P L+ L G +++ P+A W + R A+ Y
Sbjct: 167 RFPELSLLYANQLGCQLLLFPAAFNPKTGSLHWELLGRARALDTQCY 213
>UniRef50_Q5C342 Cluster: SJCHGC04680 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC04680 protein - Schistosoma
japonicum (Blood fluke)
Length = 238
Score = 46.0 bits (104), Expect = 0.001
Identities = 60/212 (28%), Positives = 88/212 (41%), Gaps = 23/212 (10%)
Frame = +3
Query: 138 FEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAV 317
F + K I+ A A V I+ L E F F + + AE VL GP LA
Sbjct: 32 FNQAVKYINKAIASGVKIVFLPEC----FDFVVLSHKETLNLAE-VLKGPLVTRYCSLAA 86
Query: 318 KYDMVI-ISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI------------PRV- 455
+ ++ I + + + D I+N+ +VIN G+++G + K H+ P +
Sbjct: 87 RENLWISLGGAHIKSSDNDDQIYNSHIVINSDGQIVGVYHKVHLFDANLNAEEITTPNIK 146
Query: 456 ----GDFNESTYYFEGNTGHPVFE-TKYGKVAINICYGRHHP--LNWLMFGINGAEIVFN 614
F ES G V E T G + + ICY P ++L + N A ++
Sbjct: 147 STCTQSFCESKVTRSGMEAPNVIENTPIGNLGLAICYDLRFPELASYLRYARN-AHVIAY 205
Query: 615 PSA--TVSGLSEHLWAVEARNAAIANSYYTCA 704
PSA T +G S H W R AI N Y A
Sbjct: 206 PSAFSTRTGESGH-WHTLLRARAIENQCYIVA 236
>UniRef50_Q5R0H6 Cluster: Predicted amidohydrolase, nitrilase
family; n=2; Idiomarina|Rep: Predicted amidohydrolase,
nitrilase family - Idiomarina loihiensis
Length = 265
Score = 45.6 bits (103), Expect = 0.001
Identities = 49/206 (23%), Positives = 83/206 (40%), Gaps = 5/206 (2%)
Frame = +3
Query: 102 TDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLT 281
+ P Q LAI V K++ A + ++ L EA++ F R + AEP
Sbjct: 2 SSRPDPQDNLAI---VAKLLEQLPAARPQLVVLPEAFSC-FGAGDRAQLA---MAEPYKD 54
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
G LA LA K+++ ++ L D G+ +++ G ++ ++ K H+ V
Sbjct: 55 GEVQKQLAALAKKHEVYLVGGTLPVDA--GERFSAASILFGPDGAILNRYDKIHLFDVDV 112
Query: 462 FNESTYYFEGNTGHP-----VFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSAT 626
+ + Y E P ET +G V + +CY P + G++I+ PSA
Sbjct: 113 ADNTKEYRESKWTQPGSKVVTTETDFGVVGMAVCYDLRFPELFRALRQAGSQIIVLPSAF 172
Query: 627 VSGLSEHLWAVEARNAAIANSYYTCA 704
+ W R AI + A
Sbjct: 173 TQVTGKAHWHALVRARAIEQQVFIVA 198
>UniRef50_Q2NTW0 Cluster: Putative uncharacterized protein; n=2;
Sodalis glossinidius str. 'morsitans'|Rep: Putative
uncharacterized protein - Sodalis glossinidius (strain
morsitans)
Length = 271
Score = 45.6 bits (103), Expect = 0.001
Identities = 35/138 (25%), Positives = 62/138 (44%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
L++LA + + II+ +ER + G + V E + RK H+ GD E +
Sbjct: 72 LSQLAGELGLHIIAGFVERGERTGQVYNSAGVWAPEGQSWLHAQRKIHL--WGD--EKKW 127
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAV 659
+ EG + + T GK+ + +CY P +F + +I+F A S ++W +
Sbjct: 128 FSEGEQ-YEIIATPLGKIGVMVCYDLGFPEVARIFALRQVDILF-VIAAWSEAEAYIWDI 185
Query: 660 EARNAAIANSYYTCAINR 713
A+ N + A+NR
Sbjct: 186 NCAARALENGVFLVAVNR 203
>UniRef50_Q4Q8W4 Cluster: Nitrilase, putative; n=6;
Trypanosomatidae|Rep: Nitrilase, putative - Leishmania
major
Length = 279
Score = 45.6 bits (103), Expect = 0.001
Identities = 40/199 (20%), Positives = 80/199 (40%), Gaps = 5/199 (2%)
Frame = +3
Query: 114 ITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPST 293
+T+++ A +K +I+ AA + L E +N P+ + + +++E + G T
Sbjct: 14 VTREKAANIKKAVTMITEAAKRGSKLAVLPECFNCPYG-----TKYFDEYSEALAPGNET 68
Query: 294 VFLAELAVKYDMV-IISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD--- 461
K + + I++ + G ++N+++ G + HRK H+ +
Sbjct: 69 FDAMSQCAKANSIWIVAGSIPEKSADGK-LFNSSMTFGSDGALKHVHRKVHLFCINTDTV 127
Query: 462 -FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGL 638
F+ES GN + ++ K + IC+ +P + G + P A
Sbjct: 128 RFDESEVLSAGNDATAISLDEHTKFGVAICFDIRYPFLAWKYAEQGTSFIVYPGAFNMVT 187
Query: 639 SEHLWAVEARNAAIANSYY 695
W + AR A+ N Y
Sbjct: 188 GPMHWQLAARARAVDNQQY 206
>UniRef50_A6Q8M5 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Sulfurovum sp. NBC37-1|Rep: Carbon-nitrogen
hydrolase family protein - Sulfurovum sp. (strain
NBC37-1)
Length = 377
Score = 45.2 bits (102), Expect = 0.002
Identities = 41/189 (21%), Positives = 82/189 (43%), Gaps = 2/189 (1%)
Frame = +3
Query: 63 VRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTRE 242
+RLG+ Q A+S + + ++++ I A + + +L E + +
Sbjct: 63 IRLGIYQAQ-AVSGEGATAKN----LKRMEHAIRLAKEKHIQLLSFPELYIPGYTLSPAM 117
Query: 243 KQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTI--WNTAVVINEFGK 416
+ F + GP+ ELA + ++ I+ P E+ T+ +++ VI+E GK
Sbjct: 118 VKKVAQFKD----GPAVTKARELARRNNIAILLPYAEKAKHSDGTLAYYDSIAVIDEHGK 173
Query: 417 VIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGING 596
++ +RK H+ G + F GN + V+ V + CY P + + G
Sbjct: 174 LLNSYRKTHL--YGQQERDNWSF-GNGDYQVYHFFGFPVGVLNCYECEFPELSRILALKG 230
Query: 597 AEIVFNPSA 623
A+++ P+A
Sbjct: 231 AKLIVGPTA 239
>UniRef50_A5GU42 Cluster: Nitrilase-related protein; n=1;
Synechococcus sp. RCC307|Rep: Nitrilase-related protein
- Synechococcus sp. (strain RCC307)
Length = 305
Score = 45.2 bits (102), Expect = 0.002
Identities = 34/126 (26%), Positives = 63/126 (50%), Gaps = 9/126 (7%)
Frame = +3
Query: 96 ISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPV 275
+S + + +Q++ + + Q + A + +L L E WN P+ + + + +FAEP+
Sbjct: 14 VSPEPQVNRQQVCHWLE-QAMTQAGTSSSPKLLMLPEVWNSPY-----QAERFAEFAEPI 67
Query: 276 -------LTGPSTVF--LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGK 428
GPS +A+ AV + + +I+ + G I+NTA VI+ G ++ K
Sbjct: 68 PELGADLRDGPSDSLKVVADFAVSHRVSVIAGSIPECSSDG-RIFNTATVISPAGCLLAK 126
Query: 429 HRKNHI 446
HRK H+
Sbjct: 127 HRKMHL 132
>UniRef50_A3Y529 Cluster: Putative uncharacterized protein; n=1;
Marinomonas sp. MED121|Rep: Putative uncharacterized
protein - Marinomonas sp. MED121
Length = 277
Score = 45.2 bits (102), Expect = 0.002
Identities = 31/108 (28%), Positives = 46/108 (42%), Gaps = 1/108 (0%)
Frame = +3
Query: 393 VVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHP-L 569
V + G+ + + K H GD E Y+ GN VFE + I ICY P L
Sbjct: 103 VTLGRNGEYLTHYDKIHTAEYGDAAELKYFKRGNH-LSVFEVDGVRAGIIICYDMRFPEL 161
Query: 570 NWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
+ G +++ +P A LS H W + A+ N Y +IN+
Sbjct: 162 IRRLCGEFSVDVILHPVAFAQDLSFHTWKQFVVSRALENQVYFMSINQ 209
>UniRef50_Q4WEA8 Cluster: Hydrolase, carbon-nitrogen family,
putative; n=1; Aspergillus fumigatus|Rep: Hydrolase,
carbon-nitrogen family, putative - Aspergillus fumigatus
(Sartorya fumigata)
Length = 321
Score = 45.2 bits (102), Expect = 0.002
Identities = 29/92 (31%), Positives = 44/92 (47%), Gaps = 2/92 (2%)
Frame = +3
Query: 351 ERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI--PRVGDFNESTYYFEGNTGHPVFETKY 524
E D G ++NTA I+ G ++G ++K +I P S G H VF+T
Sbjct: 121 EADGKEGFNLYNTAYFISNDGSILGSYQKKNIWHPERPHLTSS-----GEAPHEVFDTPI 175
Query: 525 GKVAINICYGRHHPLNWLMFGINGAEIVFNPS 620
GKV + IC+ P + +GAE+V P+
Sbjct: 176 GKVGLLICWDLAFPEAFRELIASGAEVVIIPT 207
>UniRef50_Q7UWX1 Cluster: Beta-alanine synthetase; n=1; Pirellula
sp.|Rep: Beta-alanine synthetase - Rhodopirellula
baltica
Length = 303
Score = 44.8 bits (101), Expect = 0.002
Identities = 37/140 (26%), Positives = 72/140 (51%)
Frame = +3
Query: 144 KVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKY 323
+++ + A+A+ I+CL E + + + + A P+ G T L+E+A K
Sbjct: 75 RIENAVEEASAKGAEIVCLPET--CLYGWVNAKAH---ELAHPI-PGKDTDALSEIAKKN 128
Query: 324 DMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGH 503
+ + + E++ GD ++++ V+I++ G++I KHRK + V S Y G++
Sbjct: 129 RVFLSVGLSEKE---GDQLYDSVVLIDDEGELILKHRKMN---VLTHLMSPPYTRGDSVE 182
Query: 504 PVFETKYGKVAINICYGRHH 563
+ ETK+G+V + IC H
Sbjct: 183 -IVETKFGRVGMLICADTFH 201
>UniRef50_Q18UY7 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Desulfitobacterium hafniense|Rep: Nitrilase/cyanide
hydratase and apolipoprotein N-acyltransferase -
Desulfitobacterium hafniense (strain DCB-2)
Length = 356
Score = 44.8 bits (101), Expect = 0.002
Identities = 40/168 (23%), Positives = 77/168 (45%), Gaps = 11/168 (6%)
Frame = +3
Query: 240 EKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKV 419
+ Q + D + G T L + A +Y+ +I+ + +N+A +I+ G+V
Sbjct: 68 DHQYYVDHMAIDIPGEETELLGKKAKEYNAYLIAQAKVKHPEFPGRFFNSAFLIDPKGEV 127
Query: 420 IGKHRK--------NHIPR-VGDFNESTYYFEGNTGHPVFETKYGKVAINICY--GRHHP 566
I + K + +P V D Y ++ ++ + V +T+ G++ + +C H P
Sbjct: 128 ILQSYKMQVFCQEHSTVPHDVWDKWIELYGYKLDSFYSVADTEIGRIGLLVCQEGDYHEP 187
Query: 567 LNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAIN 710
L +NGAEI++ SA ++ W ++ R A+ N+ Y A N
Sbjct: 188 ARGL--AMNGAEIIYRSSAPEPAVANGWWELQNRARALDNTCYVVAPN 233
>UniRef50_Q9LE50 Cluster: Nitrilase 1 like protein; n=2; Arabidopsis
thaliana|Rep: Nitrilase 1 like protein - Arabidopsis
thaliana (Mouse-ear cress)
Length = 316
Score = 44.8 bits (101), Expect = 0.002
Identities = 48/197 (24%), Positives = 81/197 (41%), Gaps = 8/197 (4%)
Frame = +3
Query: 138 FEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAV 317
F +++ AA ++C E F+F ++ AEP L GP LA
Sbjct: 54 FATCSRLVQEAALAGAKLICFPEN----FSFVGDKEGESVKIAEP-LDGPVMERYCSLAR 108
Query: 318 KYDMVI-ISPILER-DDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNEST 476
++ + + ER DD H + NT VVI++ G + ++K H +P + ES+
Sbjct: 109 DSNIWLSLGGFQERFDDTH---LCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESS 165
Query: 477 YYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGING-AEIVFNPSATVSGLSEHLW 653
+ G V ++ G++ + +CY P + A+++ PSA E W
Sbjct: 166 FTVPGTKIVSV-DSPVGRLGLTVCYDLRFPKIYQQLRFEQKAQVLLVPSAFTKVTGEAHW 224
Query: 654 AVEARNAAIANSYYTCA 704
+ R AI Y A
Sbjct: 225 EILLRARAIETQCYVIA 241
>UniRef50_Q60BT4 Cluster: Hydrolase, carbon-nitrogen family; n=15;
Proteobacteria|Rep: Hydrolase, carbon-nitrogen family -
Methylococcus capsulatus
Length = 273
Score = 44.4 bits (100), Expect = 0.003
Identities = 35/155 (22%), Positives = 67/155 (43%), Gaps = 5/155 (3%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
AE +GP FLA A ++ + ++ + G + + +V ++ G+ +G++ K H
Sbjct: 59 AETDGSGPIQEFLAGAAERHKVWLVGGTMPMCAGDG-RVRASCLVYDDHGRRVGRYDKIH 117
Query: 444 -----IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIV 608
+P + + E T V ++ +G + I ICY P + G +++
Sbjct: 118 LFDVVVPGTEETYRESLTIEPGTVPLVLDSPFGALGIAICYDLRFPELFRRMAQQGLDLL 177
Query: 609 FNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
P+A + W + R A+ N YT A N+
Sbjct: 178 AVPAAFTARTGAAHWEILVRARAVENLCYTVASNQ 212
>UniRef50_Q0LC17 Cluster: NAD+ synthetase; n=1; Herpetosiphon
aurantiacus ATCC 23779|Rep: NAD+ synthetase -
Herpetosiphon aurantiacus ATCC 23779
Length = 622
Score = 44.4 bits (100), Expect = 0.003
Identities = 22/66 (33%), Positives = 39/66 (59%)
Frame = +3
Query: 429 HRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIV 608
HRK +P G F+E+ + E F+T++G+VAI IC H L+ + ++GA+++
Sbjct: 121 HRKMFLPTYGVFDEARFV-EAGRQIAAFDTRFGRVAILICEDAWHSLSGTVAALDGAQML 179
Query: 609 FNPSAT 626
+ SA+
Sbjct: 180 YVVSAS 185
>UniRef50_A6X6J7 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Ochrobactrum
anthropi ATCC 49188|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Ochrobactrum anthropi
(strain ATCC 49188 / DSM 6882 / NCTC 12168)
Length = 279
Score = 44.4 bits (100), Expect = 0.003
Identities = 43/153 (28%), Positives = 64/153 (41%), Gaps = 6/153 (3%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
AE V GP+ + A ++ + + + L + I+N+ V N GK I +RK H
Sbjct: 57 AESVPGGPAYKMAQDFAREHKVFVHAGTLMEKVPNEKRIYNSTFVFNREGKEIAHYRKIH 116
Query: 444 I-PRVGDFNESTYYFEGNTGHP-----VFETKYGKVAINICYGRHHPLNWLMFGINGAEI 605
+ VG + T Y E T P V++ KV ICY +L GA++
Sbjct: 117 MFDIVGP--DGTAYKESATVKPGENVVVYDLDGFKVGCAICYDIRFAELYLELEKAGADV 174
Query: 606 VFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
+ P+A + W V AR AI Y A
Sbjct: 175 IVLPAAFTLQTGKDHWEVLARARAIETQTYFAA 207
>UniRef50_A5G317 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=5;
Proteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Acidiphilium cryptum
(strain JF-5)
Length = 284
Score = 44.4 bits (100), Expect = 0.003
Identities = 47/193 (24%), Positives = 79/193 (40%), Gaps = 10/193 (5%)
Frame = +3
Query: 156 IISAAAAEQVNILCLQEAWNM---PFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYD 326
I +A AA++ ++ L E W+ A T + G + FL E A ++
Sbjct: 31 IDAAVAADRPGLVSLPEVWSCLGGDRAAKTEAAEVLPAAGSGETGGDAYEFLRETARRHR 90
Query: 327 MVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI-----PRVGDFNESTYYFEG 491
+ + + GD ++NT +V + G+ I ++RK H+ P + ES Y
Sbjct: 91 IHVHGGSIGEQG--GDRLYNTTLVFDPDGREIARYRKIHLFDITTPDGQGYRESATY--- 145
Query: 492 NTGHPVFETKYG--KVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEA 665
G V + G V ++ICY P +L GA+++ P+A + W V
Sbjct: 146 GAGDAVVTCRIGGLTVGLSICYDMRFPELYLALHRAGADLIMVPAAFTLQTGKDHWDVLL 205
Query: 666 RNAAIANSYYTCA 704
R AI + A
Sbjct: 206 RARAIETQCWIAA 218
>UniRef50_A0JW88 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2; Arthrobacter|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Arthrobacter sp. (strain FB24)
Length = 363
Score = 44.4 bits (100), Expect = 0.003
Identities = 42/161 (26%), Positives = 72/161 (44%), Gaps = 16/161 (9%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPI-LERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPR 452
+ GP T LA+ AV+ + I + + + +D+ D +N A +I+ G++I K K
Sbjct: 79 IPGPETDELAKKAVELNTYIAAELYMVKDEDFPDRHFNVAFIIDPQGEIIYKRYKATSDA 138
Query: 453 -----VGDFNESTYYFE-----GNTG-----HPVFETKYGKVAINICYGRHHPLNWLMFG 587
+G+ N + E GN PV +T+ G + IC+ +P
Sbjct: 139 YEGGMLGNMNPHDVWDEWIEKKGNGNAMDAIFPVAKTEIGNIGYAICHEGVYPEVPRGLA 198
Query: 588 INGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAIN 710
+NGAEI+ + + +W ++ R A+ NS Y A N
Sbjct: 199 MNGAEIIIRGTLIEPAVQNGMWELQNRAHAMFNSAYIVAPN 239
>UniRef50_P55176 Cluster: UPF0012 hydrolase in pqqF 5'region; n=11;
Pseudomonas|Rep: UPF0012 hydrolase in pqqF 5'region -
Pseudomonas fluorescens
Length = 285
Score = 44.4 bits (100), Expect = 0.003
Identities = 39/143 (27%), Positives = 61/143 (42%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
GPS +A +A I+ ER + G I+N +I+ G+ + +RK H+ GD
Sbjct: 82 GPSAQRIAAIAQAAGTAILYGYPERS-VDGQ-IYNAVQLIDAQGQRLCNYRKTHL--FGD 137
Query: 462 FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLS 641
+ S + G P+ E K+ ICY P N + GAE++ P+A +
Sbjct: 138 LDHSMFS-AGEDDFPLVELDGWKLGFLICYDIEFPENARRLALAGAELILVPTANMIPY- 195
Query: 642 EHLWAVEARNAAIANSYYTCAIN 710
+ + V R A N Y N
Sbjct: 196 DFVADVTIRARAFENQCYVAYAN 218
>UniRef50_A7FDR9 Cluster: Hydrolase, carbon-nitrogen family protein;
n=16; Enterobacteriaceae|Rep: Hydrolase, carbon-nitrogen
family protein - Yersinia pseudotuberculosis IP 31758
Length = 289
Score = 44.0 bits (99), Expect = 0.004
Identities = 41/150 (27%), Positives = 70/150 (46%), Gaps = 9/150 (6%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVI---ISPILERDDIHGDTIWNTAVVINEFGKVIGKHR 434
AE GP + E+A +Y + I P++ R+ D I +++++ ++ G++ ++
Sbjct: 57 AEQHNDGPLQQEVREMARRYGVWIQVGSMPMVSRES--PDLITSSSLLFDDQGELKARYD 114
Query: 435 KNHIPRVGDFNE-STYYFEGNTGHP-----VFETKYGKVAINICYGRHHPLNWLMFGING 596
K H+ V D N+ +Y E +T P V +T G++ + ICY P + G
Sbjct: 115 KIHMFDV-DINDIHGHYRESDTYQPGQQLTVVDTPVGRLGMTICYDLRFPGLFQALRAQG 173
Query: 597 AEIVFNPSATVSGLSEHLWAVEARNAAIAN 686
AEI+ P+A E W R AI N
Sbjct: 174 AEIISVPAAFTKMTGEAHWETLLRARAIEN 203
>UniRef50_A0LQU6 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Acidothermus
cellulolyticus 11B|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Acidothermus
cellulolyticus (strain ATCC 43068 / 11B)
Length = 272
Score = 44.0 bits (99), Expect = 0.004
Identities = 56/203 (27%), Positives = 89/203 (43%), Gaps = 13/203 (6%)
Frame = +3
Query: 141 EKVQKIIS-AAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPV--LTGPSTVFLAEL 311
++V +++ A+ +++ L E W +P AF +R FAE L GP L +
Sbjct: 18 DRVDRVVDLVASCRDADLVVLPELW-VPGAFASRF------FAEVATELPGPIIPRLGAV 70
Query: 312 AVKYDMVIIS-PILERDDIHGDTI-WNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYF 485
A + I++ +ER D D I +NTAV++N G + +RK H+ + E+
Sbjct: 71 AKELGAFIMAGTFIERADPATDRIGYNTAVLLNPDGAIAHTYRKVHLFGFHE-GEARMLA 129
Query: 486 EGN-------TGHPVFET-KYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLS 641
GN G + ET YG + CY P + + G +++ PS +
Sbjct: 130 AGNDVTTCRLEGGRMTETATYG---TSTCYDLRFPELYRILVDQGCDLLVIPSGWPAQRL 186
Query: 642 EHLWAVEARNAAIANSYYTCAIN 710
EH W V R AI N + A N
Sbjct: 187 EH-WRVLTRARAIENQLFVVACN 208
>UniRef50_UPI0000E472D9 Cluster: PREDICTED: similar to
Ureidopropionase, beta, partial; n=1; Strongylocentrotus
purpuratus|Rep: PREDICTED: similar to Ureidopropionase,
beta, partial - Strongylocentrotus purpuratus
Length = 57
Score = 43.6 bits (98), Expect = 0.005
Identities = 18/41 (43%), Positives = 27/41 (65%)
Frame = +3
Query: 3 FEIKAYDFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQ 125
FE+K + A E+ R PR+VR+GLIQ+ I + T P+ +Q
Sbjct: 17 FEVKGHQIAAGCEQLRSPRLVRIGLIQNQIVLPTTAPVKEQ 57
>UniRef50_Q89E80 Cluster: Bll7207 protein; n=1; Bradyrhizobium
japonicum|Rep: Bll7207 protein - Bradyrhizobium
japonicum
Length = 307
Score = 43.6 bits (98), Expect = 0.005
Identities = 25/88 (28%), Positives = 45/88 (51%), Gaps = 12/88 (13%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHI-----PRVG---DFNESTYYFEGNTGHPVFET----KY 524
+N A++++ G+++G++RK H+ PR G E Y+ G+ G P F +
Sbjct: 94 YNCAILVDRDGEILGRYRKVHLPGSVEPRPGARYQQLEKRYFEYGDLGFPAFRAGSAWAH 153
Query: 525 GKVAINICYGRHHPLNWLMFGINGAEIV 608
+ + IC R P +W + G+ G E+V
Sbjct: 154 AIMGMMICNDRRWPESWRVLGLQGVELV 181
>UniRef50_Q28TG7 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2; Jannaschia sp.
CCS1|Rep: Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Jannaschia sp. (strain CCS1)
Length = 298
Score = 43.6 bits (98), Expect = 0.005
Identities = 31/112 (27%), Positives = 50/112 (44%), Gaps = 5/112 (4%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNHIPRV-----GDFNESTYYFEGNTGHPVFETKYGKVAINIC 548
N V++ G ++ ++ K H+ V ES Y G+ V +T +G +A++IC
Sbjct: 99 NHTVLVAPSGDIVARYDKIHLFDVFLDGRRATGESDRYAPGSEA-VVADTPFGPMALSIC 157
Query: 549 YGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
Y P + + + G+ ++F PSA W V R AI N Y A
Sbjct: 158 YDLRFPHLYRDYALAGSTVMFIPSAFTVPTGRAHWEVLLRARAIENGAYVIA 209
>UniRef50_Q127K6 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=12; root|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Polaromonas sp. (strain JS666 / ATCC
BAA-500)
Length = 313
Score = 43.6 bits (98), Expect = 0.005
Identities = 34/116 (29%), Positives = 53/116 (45%), Gaps = 1/116 (0%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPILERD-DIHGDTIWNTAVVINEFGKVIGKHRKNHIPR 452
L G L E A +++ I+ I ERD + G T++NT V I G+V +HRK +P
Sbjct: 78 LGGGDLAELCEAARAHNVTIVCGINERDRERGGGTLYNTVVTIGADGRVQNRHRK-LMPT 136
Query: 453 VGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPS 620
+ + +G V +T G++ IC+ + PL G EI P+
Sbjct: 137 --NPERMVHGLGDASGLRVVDTPAGRIGCLICWENYMPLARYALYAQGVEIYIAPT 190
>UniRef50_Q0SBF1 Cluster: Probable nitrilase; n=2;
Actinomycetales|Rep: Probable nitrilase - Rhodococcus
sp. (strain RHA1)
Length = 318
Score = 43.6 bits (98), Expect = 0.005
Identities = 35/116 (30%), Positives = 57/116 (49%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRV 455
L GP + ++A D+VI E D G +N AV ++ G ++G +RK H P
Sbjct: 76 LDGPEIRRVVDMAG--DLVITLGFCEAD---GADRYNAAVTVHGDG-ILGSYRKVHQP-- 127
Query: 456 GDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
E+ Y G+ + F+T G++ + ICY + P ++GAEI+ + SA
Sbjct: 128 --LGENLCYRAGDK-YEAFDTPVGRMGMQICYDKAFPEAARTLALDGAEIITSLSA 180
>UniRef50_A6DN63 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Lentisphaera
araneosa HTCC2155|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Lentisphaera araneosa
HTCC2155
Length = 292
Score = 43.6 bits (98), Expect = 0.005
Identities = 33/141 (23%), Positives = 63/141 (44%), Gaps = 6/141 (4%)
Frame = +3
Query: 291 TVFLAELAVKYDMVII-SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI-----PR 452
T L+ L+ Y + I+ + ER + + ++N++ + + G ++ +RK H+ P
Sbjct: 87 TDLLSPLSKTYKIAIVWGGLAERQE---NKVFNSSFIFDADGHLLDVYRKTHLFQIFTPG 143
Query: 453 VGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVS 632
+E+ Y G+TG V + + I+ICY P + G +++ N +A
Sbjct: 144 KKAIDETETYEHGDTGPCVVKINDWSIGISICYDLRFP--EFLRNYAGCDLMINSAAFTK 201
Query: 633 GLSEHLWAVEARNAAIANSYY 695
+ W V R A+ N Y
Sbjct: 202 ATGKAHWEVLMRARAVENQSY 222
>UniRef50_P47016 Cluster: Probable hydrolase NIT2; n=6;
Saccharomycetales|Rep: Probable hydrolase NIT2 -
Saccharomyces cerevisiae (Baker's yeast)
Length = 307
Score = 43.6 bits (98), Expect = 0.005
Identities = 35/124 (28%), Positives = 52/124 (41%), Gaps = 6/124 (4%)
Frame = +3
Query: 342 PILERDDIHG-DTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFEGNTGH 503
P E+D + G D + N + I+ GK++ +++K H +P ES G
Sbjct: 95 PPSEQDLLEGNDRVRNVLLYIDHEGKILQEYQKLHLFDVDVPNGPILKESKSVQPGKAIP 154
Query: 504 PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIA 683
+ E+ GK+ ICY P L GAEI+ PSA E W + R A+
Sbjct: 155 DIIESPLGKLGSAICYDIRFPEFSLKLRSMGAEILCFPSAFTIKTGEAHWELLGRARAVD 214
Query: 684 NSYY 695
Y
Sbjct: 215 TQCY 218
>UniRef50_O76463 Cluster: Nitrilase and fragile histidine triad
fusion protein NitFhit [Includes:
Bis(5'-adenosyl)-triphosphatase (EC 3.6.1.29)
(Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
(Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
Nitrilase homolog (EC 3.5.-.-)]; n=4; Bilateria|Rep:
Nitrilase and fragile histidine triad fusion protein
NitFhit [Includes: Bis(5'-adenosyl)-triphosphatase (EC
3.6.1.29) (Diadenosine 5',5'''-P1,P3-triphosphate
hydrolase) (Dinucleosidetriphosphatase) (AP3A hydrolase)
(AP3Aase); Nitrilase homolog (EC 3.5.-.-)] -
Caenorhabditis elegans
Length = 440
Score = 43.6 bits (98), Expect = 0.005
Identities = 37/139 (26%), Positives = 58/139 (41%), Gaps = 6/139 (4%)
Frame = +3
Query: 306 ELAVKYDMVI-ISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFN 467
ELA K+++ + + + +D WNT ++I+ G ++ K H IP
Sbjct: 83 ELARKHNIWLSLGGLHHKDPSDAAHPWNTHLIIDSDGVTRAEYNKLHLFDLEIPGKVRLM 142
Query: 468 ESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEH 647
ES + G P +T G++ ++ICY P L GA+++ PSA
Sbjct: 143 ESEFSKAGTEMIPPVDTPIGRLGLSICYDVRFPELSLWNRKRGAQLLSFPSAFTLNTGLA 202
Query: 648 LWAVEARNAAIANSYYTCA 704
W R AI N Y A
Sbjct: 203 HWETLLRARAIENQCYVVA 221
>UniRef50_Q15ZG7 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Alteromonadales|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Pseudoalteromonas
atlantica (strain T6c / BAA-1087)
Length = 276
Score = 43.2 bits (97), Expect = 0.007
Identities = 38/154 (24%), Positives = 68/154 (44%), Gaps = 7/154 (4%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
AE + GP L +A +Y + +++ + + D + ++IN+ G+ + +++K H
Sbjct: 57 AESLGDGPIQARLMGMAKQYGVWLVAGSMPLKSENPDKFTASCLLINDAGERVTEYQKIH 116
Query: 444 I--PRVGD----FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHP-LNWLMFGINGAE 602
+ +V D + ES Y G+T V +T +G + + ICY P L M +
Sbjct: 117 LFDVQVADNTKTYCESKYTQAGSTLVSVPDTPFGHLGLAICYDVRFPGLFQAMAEHKALD 176
Query: 603 IVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
++ P+A E W AI N Y A
Sbjct: 177 VIALPAAFTQKTGEAHWQALLSARAIENQCYLVA 210
>UniRef50_Q5KLT5 Cluster: Nitrilase-like protein, putative; n=2;
Filobasidiella neoformans|Rep: Nitrilase-like protein,
putative - Cryptococcus neoformans (Filobasidiella
neoformans)
Length = 356
Score = 43.2 bits (97), Expect = 0.007
Identities = 57/220 (25%), Positives = 89/220 (40%), Gaps = 18/220 (8%)
Frame = +3
Query: 99 STDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVL 278
ST +P+ +++ +K+I A A L EA + F K F+ P+
Sbjct: 53 STSDPVHNLKIS-----EKVIRNAVAAGAKACFLPEASD----FINPSKTESRKFSHPLP 103
Query: 279 TGPSTVFLAELAVKYDMVIISPILER-DDIHGDTIWNTAVVINEFGKVIGKHRKNHI--- 446
T+ L LA + +VI + E +D + ++NT V+I + G ++ +RK H+
Sbjct: 104 KHEYTIGLQRLAKELGIVISVGVHEGPEDESEERVYNTHVLIGKDGGILASYRKIHLFDV 163
Query: 447 ----PRVGD--------FNESTYYFEGNTGHPVFETK-YGKVAINICYGRHHPLNWLMFG 587
P D ES G P E + G + + ICY P ++
Sbjct: 164 ELSKPPAPDGTPRPPQRTGESERILAGQAVTPPVEVEGIGNIGLEICYDIRFPELSIILT 223
Query: 588 INGAEIVFNPSA-TVSGLSEHLWAVEARNAAIANSYYTCA 704
GAE++ PSA TV +H W R AI Y A
Sbjct: 224 RLGAEVLLFPSAFTVKTGRDH-WGTLCRATAIQYQSYLIA 262
>UniRef50_Q5K7Z3 Cluster: Expressed protein; n=1; Filobasidiella
neoformans|Rep: Expressed protein - Cryptococcus
neoformans (Filobasidiella neoformans)
Length = 337
Score = 43.2 bits (97), Expect = 0.007
Identities = 33/127 (25%), Positives = 57/127 (44%)
Frame = +3
Query: 270 PVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIP 449
P+ +GP + +++ + L ++ + NTA I+E G + G++ K ++
Sbjct: 120 PLKSGPRPSKVTLQQLEWAKYLEQHPLSSEENSTPIVKNTAFFIDEEGVLQGEYVKQNLW 179
Query: 450 RVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATV 629
E Y G VFETK+GK + IC+ HP G +I+F P+ +
Sbjct: 180 HP----EREYIVAGIEPRQVFETKWGKAGLLICWDMSHPSAAQELADLGVDIIFAPTYWM 235
Query: 630 SGLSEHL 650
+ SE L
Sbjct: 236 ATDSEPL 242
>UniRef50_Q6KZW3 Cluster: Carbon-nitrogen hydrolase; n=1;
Picrophilus torridus|Rep: Carbon-nitrogen hydrolase -
Picrophilus torridus
Length = 239
Score = 43.2 bits (97), Expect = 0.007
Identities = 28/83 (33%), Positives = 44/83 (53%)
Frame = +3
Query: 372 DTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICY 551
+ ++N + +I++ G +IG K ++ ES YY GN + VFET +GK+ I ICY
Sbjct: 72 EKLFNRSYIISD-GALIGYQDKINLY----MGESIYYNPGNKIN-VFETMHGKIGIAICY 125
Query: 552 GRHHPLNWLMFGINGAEIVFNPS 620
P + GA ++ NPS
Sbjct: 126 DLDFPYYAKILIKKGASLILNPS 148
>UniRef50_O25836 Cluster: Formamidase; n=17; Bacteria|Rep:
Formamidase - Helicobacter pylori (Campylobacter pylori)
Length = 334
Score = 43.2 bits (97), Expect = 0.007
Identities = 36/144 (25%), Positives = 66/144 (45%), Gaps = 1/144 (0%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
G T A+ + + + I+ER+ +NTA++I+ G++I K+RK +
Sbjct: 82 GKETELYAKACKEAKVYGVFSIMERNPDSNKNPYNTAIIIDPQGEIILKYRK-----LFP 136
Query: 462 FNESTYYFEGNTGHPVFETKYG-KVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGL 638
+N ++ G+ G PV E G K+A+ IC+ P G + S + +
Sbjct: 137 WNPIEPWYPGDLGMPVCEGPGGSKLAVCICHDGMIPELAREAAYKGCNVYIRISGYSTQV 196
Query: 639 SEHLWAVEARNAAIANSYYTCAIN 710
++ W + R+ A N YT ++N
Sbjct: 197 NDQ-WILTNRSNAWHNLMYTVSVN 219
>UniRef50_Q0S9R8 Cluster: Probable formamidase; n=1; Rhodococcus sp.
RHA1|Rep: Probable formamidase - Rhodococcus sp. (strain
RHA1)
Length = 299
Score = 42.7 bits (96), Expect = 0.009
Identities = 39/155 (25%), Positives = 66/155 (42%), Gaps = 2/155 (1%)
Frame = +3
Query: 252 WCDFAEPVLTGPSTVFLAELAVKYDMVII-SPILERDDIHGDTIWNTAVVINEFGKVIGK 428
W D L GP + LA + + ++ + ER D D I+NTA+ ++ G+V+ +
Sbjct: 62 WMDKVALPLAGPHIDRICALAEETGLWLVPGSLYERGD--DDKIYNTAIAVSPLGEVVAR 119
Query: 429 HRKNHIPRVGDFNESTYYFEGNTGHPVFETK-YGKVAINICYGRHHPLNWLMFGINGAEI 605
+RK V + G+ VF+ G++ + ICY P GAE+
Sbjct: 120 YRK-----VFPWQPYEQTAPGSE-FVVFDIPGIGRIGLAICYDGSFPETARQLAWLGAEV 173
Query: 606 VFNPSATVSGLSEHLWAVEARNAAIANSYYTCAIN 710
+ P+ T + + V +R A N Y +N
Sbjct: 174 IIQPTLTTT-RDREMELVCSRANAWTNQVYVVNVN 207
>UniRef50_A3H5Q5 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Caldivirga
maquilingensis IC-167|Rep: Nitrilase/cyanide hydratase
and apolipoprotein N-acyltransferase - Caldivirga
maquilingensis IC-167
Length = 284
Score = 42.7 bits (96), Expect = 0.009
Identities = 36/125 (28%), Positives = 66/125 (52%), Gaps = 4/125 (3%)
Frame = +3
Query: 258 DFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRK 437
+ A+P+ GP + LA+ A + + + + + ER +G I++ AV ++ G ++ K+RK
Sbjct: 58 ELAKPI-PGPYSDALADAARESGIYVAAGLTER---YGGRIYDAAVFLSPKGDLLWKYRK 113
Query: 438 -NHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLM---FGINGAEI 605
N +P +S Y G V ET+YG++ +NIC + P N ++ GA +
Sbjct: 114 INLLPD----EQSIYEVGDRVG--VVETEYGRIGVNICID-NAPSNLVLAHSMARMGAVM 166
Query: 606 VFNPS 620
+ +PS
Sbjct: 167 ILSPS 171
>UniRef50_Q88EJ9 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Pseudomonas putida KT2440|Rep: Carbon-nitrogen
hydrolase family protein - Pseudomonas putida (strain
KT2440)
Length = 273
Score = 42.3 bits (95), Expect = 0.011
Identities = 38/151 (25%), Positives = 62/151 (41%), Gaps = 5/151 (3%)
Frame = +3
Query: 267 EPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI 446
EP GP+ +LA ++ + + G ++NT+VV + G +G++RK H+
Sbjct: 58 EPHSGGPAYEMCKKLAQDCNVYVHTGSFYESTPDGSRVYNTSVVFDPKGNELGRYRKIHL 117
Query: 447 -----PRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVF 611
P + ES+ G T V + + K ICY P + GA+++
Sbjct: 118 FDIVTPDGMRYGESSAVAPG-TEVSVVDIEGLKYGFAICYDIRFPELFQKLVALGADVIV 176
Query: 612 NPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
P+A + W V R AI Y A
Sbjct: 177 LPAAFTLQTGKDHWDVLCRARAIETQCYFLA 207
>UniRef50_A6T0X3 Cluster: Nitrilase; n=7; Bacteria|Rep: Nitrilase -
Janthinobacterium sp. (strain Marseille) (Minibacterium
massiliensis)
Length = 316
Score = 42.3 bits (95), Expect = 0.011
Identities = 32/116 (27%), Positives = 52/116 (44%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRV 455
L GP +AE M + +ER+ T++ T + N ++GKHRK +P
Sbjct: 81 LKGPEVEAIAEATRNTGMFAVIGCIEREL---GTLYCTVLFFNGAQGLVGKHRKL-MPTA 136
Query: 456 GDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
G+ + F + PVF+T GK+ IC+ + P+ + G I P+A
Sbjct: 137 GE--RLIWGFGDGSTMPVFDTPLGKIGAVICWENYMPMLRMYMYSQGIGIYCAPTA 190
>UniRef50_A4YSE7 Cluster: N-carbamoyl-D-amino acid hydrolase; n=11;
Proteobacteria|Rep: N-carbamoyl-D-amino acid hydrolase -
Bradyrhizobium sp. (strain ORS278)
Length = 318
Score = 42.3 bits (95), Expect = 0.011
Identities = 22/83 (26%), Positives = 41/83 (49%), Gaps = 7/83 (8%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRVGDFNE-------STYYFEGNTGHPVFETKYGKVAI 539
+NTA+++++ +++ K+RK H+P + YFE G V + G + +
Sbjct: 109 YNTAILVDKDARIVSKYRKVHLPGHAEHEPWRKFQHLEKRYFEPGRGFGVADAFGGVIGM 168
Query: 540 NICYGRHHPLNWLMFGINGAEIV 608
IC R P + + G+ G E+V
Sbjct: 169 AICNDRRWPETYRVMGLQGVEMV 191
>UniRef50_A3JKT7 Cluster: Acetyltransferase domain (GNAT family)
fused to predicted amidohydrolase (Nitrilase family)
protein; n=2; Alteromonadales|Rep: Acetyltransferase
domain (GNAT family) fused to predicted amidohydrolase
(Nitrilase family) protein - Marinobacter sp. ELB17
Length = 508
Score = 42.3 bits (95), Expect = 0.011
Identities = 39/134 (29%), Positives = 61/134 (45%), Gaps = 2/134 (1%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
++++AV Y++ II+ + I D ++N + + + G+V + RK HI +
Sbjct: 297 MSDMAVSYNINIITGSMPL--IENDRVYNVSYLCHRDGRV-DEQRKIHIT---PHERRDW 350
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNP--SATVSGLSEHLW 653
EG VFET G+VAI ICY P M +I+ P + T +G
Sbjct: 351 VIEGGNEFKVFETDAGRVAILICYDIEFPELGRMAAEQEVDIICVPFWTDTKNGYLRVRH 410
Query: 654 AVEARNAAIANSYY 695
+AR AI N Y
Sbjct: 411 CAQAR--AIENECY 422
>UniRef50_A5AAF3 Cluster: Contig An02c0310, complete genome; n=5;
Trichocomaceae|Rep: Contig An02c0310, complete genome -
Aspergillus niger
Length = 320
Score = 42.3 bits (95), Expect = 0.011
Identities = 28/82 (34%), Positives = 41/82 (50%), Gaps = 1/82 (1%)
Frame = +3
Query: 378 IWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFE-GNTGHPVFETKYGKVAINICYG 554
++NTA I+ G ++G +RK +I E Y G+ H VF+T GKV + IC+
Sbjct: 104 LYNTAYFISNDGSILGHYRKKNIWHP----ERPYLTSSGHDPHEVFDTPIGKVGLLICWD 159
Query: 555 RHHPLNWLMFGINGAEIVFNPS 620
P + GAEIV P+
Sbjct: 160 LAFPEAFRELICKGAEIVVIPT 181
>UniRef50_Q6RWN7 Cluster: Nitrilase; n=21; root|Rep: Nitrilase -
uncultured organism
Length = 353
Score = 41.9 bits (94), Expect = 0.015
Identities = 28/94 (29%), Positives = 50/94 (53%), Gaps = 1/94 (1%)
Frame = +3
Query: 327 MVIISPILERD-DIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGH 503
+ ++ + ER+ + G +++NTA+VI G++IG+HRK + G +G+T
Sbjct: 98 VTVVIGVNERNTEASGASLYNTALVIGPLGQLIGRHRK--LVPTGPERMVWAQGDGST-L 154
Query: 504 PVFETKYGKVAINICYGRHHPLNWLMFGINGAEI 605
V++T GK++ IC+ + PL GA I
Sbjct: 155 DVYDTPVGKLSTLICWENYMPLARYAMAAWGARI 188
>UniRef50_A6DBX4 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Caminibacter
mediatlanticus TB-2|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Caminibacter
mediatlanticus TB-2
Length = 247
Score = 41.9 bits (94), Expect = 0.015
Identities = 24/79 (30%), Positives = 40/79 (50%)
Frame = +3
Query: 312 AVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEG 491
++K+D ++ +D+ I+N+A+ + G +H K H+P G F E ++F G
Sbjct: 60 SLKFDKDVVLGAAIKDE---GRIYNSALYL---GDSFHRHNKVHLPTYGVFEEGRFFFRG 113
Query: 492 NTGHPVFETKYGKVAINIC 548
F TK+GK I IC
Sbjct: 114 K-DFSCFNTKFGKTTIFIC 131
>UniRef50_Q00Y86 Cluster: Carbon-nitrogen hydrolase; n=2;
Ostreococcus|Rep: Carbon-nitrogen hydrolase -
Ostreococcus tauri
Length = 307
Score = 41.9 bits (94), Expect = 0.015
Identities = 45/156 (28%), Positives = 66/156 (42%), Gaps = 13/156 (8%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDM-VIISPILERDDIHGDTIW-NTAVVINEFGKVIGK-HRKNHI 446
L GP A +A ++ M + + + ERDD GD NT V++ G + G+ +RK H+
Sbjct: 82 LDGPIVRACAAMAREHGMWMSLGGVAERDDAGGDARRRNTHVLLTPLGTIHGEPYRKIHL 141
Query: 447 PRV-------GDFNESTYYFEGN--TGHPVFETKYGKVAINICYGRHHPLNWLMFGI-NG 596
G ES + G T H T +G V +++CY P + +G
Sbjct: 142 FDAEGVGVGGGGLMESEWTAPGRELTSHA---TDFGTVGVSVCYDVRFPDVYQALRFEHG 198
Query: 597 AEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
A+I+ PSA W V R AI Y A
Sbjct: 199 ADILIVPSAFTKITGRAHWEVLLRARAIETQCYVVA 234
>UniRef50_A1RZK0 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Thermofilum
pendens Hrk 5|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Thermofilum pendens
(strain Hrk 5)
Length = 286
Score = 41.9 bits (94), Expect = 0.015
Identities = 33/117 (28%), Positives = 51/117 (43%), Gaps = 2/117 (1%)
Frame = +3
Query: 369 GDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYG-KVAINI 545
G ++N AV+ E GKV +RK H+ + ES+ + G PV G ++ I +
Sbjct: 102 GPGVYNAAVLAEE-GKVKAVYRKIHLFDAYGYRESSVFSPGR--EPVVADLKGLRLGIAV 158
Query: 546 CYGRHHPLNWLMFGINGAEIVFNPSATVSG-LSEHLWAVEARNAAIANSYYTCAINR 713
C+ P + + GAE+ PSA G W A N+ Y A+N+
Sbjct: 159 CFDLRFPELFRSMFLRGAEVFVVPSAWYRGPYKVEQWKALTAARAHENTSYLVAVNQ 215
>UniRef50_A7I462 Cluster: Hydrolase in agr operon; n=1;
Campylobacter hominis ATCC BAA-381|Rep: Hydrolase in agr
operon - Campylobacter hominis (strain ATCC BAA-381 /
LMG 19568 / NCTC 13146 /CH001A)
Length = 256
Score = 41.5 bits (93), Expect = 0.020
Identities = 40/193 (20%), Positives = 84/193 (43%), Gaps = 2/193 (1%)
Frame = +3
Query: 138 FEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAV 317
+EKV + A +++ +I+ L E ++ F F ++ + + D + F E V
Sbjct: 19 YEKVLNFMQDAISKKTDIIVLPELFDTGF-FPSKNLEKFADKNAFRAREIFSNFARENCV 77
Query: 318 KYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNT 497
++ I E + D ++N + + ++ GK+I + K H+ G+ ES +
Sbjct: 78 N---IVAGSICE---MRNDKLFNASYIFDKNGKIIANYDKIHLFSTGNEKESEIF---TP 128
Query: 498 GHPVFETKYGKV--AINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARN 671
G + + ++ I ICY + + G ++F + +H + + A+
Sbjct: 129 GEKIISFRLNEIPCGIMICYDLRFAEIAKILALRGISVLFVVAQWPLKRIKH-FEILAKA 187
Query: 672 AAIANSYYTCAIN 710
AI N ++ CA+N
Sbjct: 188 RAIENEFFVCALN 200
>UniRef50_A5FWH4 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Acidiphilium
cryptum JF-5|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Acidiphilium cryptum
(strain JF-5)
Length = 266
Score = 41.5 bits (93), Expect = 0.020
Identities = 25/85 (29%), Positives = 43/85 (50%)
Frame = +3
Query: 369 GDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINIC 548
GD + N+A++I+E G +RK H+ GD + + G+ G PV + + + IC
Sbjct: 91 GDGVANSAILIDEAGGARLIYRKVHL--FGDLDRGMFALPGD-GFPVVAWRGLSLGLAIC 147
Query: 549 YGRHHPLNWLMFGINGAEIVFNPSA 623
Y P M + GA+++ P+A
Sbjct: 148 YDIEFPETARMMALAGADLILVPTA 172
>UniRef50_A1SE99 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=4;
Actinomycetales|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Nocardioides sp.
(strain BAA-499 / JS614)
Length = 280
Score = 41.5 bits (93), Expect = 0.020
Identities = 43/163 (26%), Positives = 69/163 (42%), Gaps = 10/163 (6%)
Frame = +3
Query: 252 WCDFAEPVLTGPSTVFLAELAVKYDMVI-ISPILERDDIHGDT------IWNTAVVINEF 410
W AE ++ GP+ +A +A + + + I+ER + D +WNT+V+I+
Sbjct: 63 WRATAE-LMNGPTIAQMASVAREVGVWLHAGSIIERAEDGADRGAERRGLWNTSVLISPQ 121
Query: 411 GKVIGKHRKNHIPRVGDFNESTYYFEGN---TGHPVFETKYGKVAINICYGRHHPLNWLM 581
G V +RK H GD E G V +T +V + CY P +
Sbjct: 122 GTVHKTYRKIHRFGFGD-GEPRVLEAGTDLAVAELVHDTGASRVGMATCYDLRFPELFRR 180
Query: 582 FGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAIN 710
G GA+++ P+A EH W + R A+ N + N
Sbjct: 181 LGDLGADVIVLPAAWPMRRVEH-WRLLGRARALENQAWVLQCN 222
>UniRef50_Q6C005 Cluster: Similar to sp|P47016 Saccharomyces
cerevisiae YJL126w NIT2 nitrilase; n=1; Yarrowia
lipolytica|Rep: Similar to sp|P47016 Saccharomyces
cerevisiae YJL126w NIT2 nitrilase - Yarrowia lipolytica
(Candida lipolytica)
Length = 289
Score = 41.5 bits (93), Expect = 0.020
Identities = 36/114 (31%), Positives = 51/114 (44%), Gaps = 6/114 (5%)
Frame = +3
Query: 372 DTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFEGNTGHPVFETKYGKVA 536
D + NT + ++ G ++ +++K H +P ES G+ FET G V
Sbjct: 98 DRVRNTLLWLDSNGDIVNRYQKVHLFDVEVPNGPILQESKSVEPGSELPKPFETPVGTVG 157
Query: 537 INICYGRHHPLNWLMFGINGAEIVFNPSA-TVSGLSEHLWAVEARNAAIANSYY 695
ICY P L+ GA+I+ PSA TV + H W V AR AI Y
Sbjct: 158 PAICYDIRFPELALLLRKQGAQILQFPSAFTVRTGAAH-WHVLARARAIDTQCY 210
>UniRef50_Q9V1L5 Cluster: Amidohydrolase, putative; n=2;
Thermococcaceae|Rep: Amidohydrolase, putative -
Pyrococcus abyssi
Length = 226
Score = 41.5 bits (93), Expect = 0.020
Identities = 33/107 (30%), Positives = 55/107 (51%)
Frame = +3
Query: 228 FCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINE 407
+C + W DF+ L G +++LA + + +I +LE + ++N+A++++
Sbjct: 44 YCLTGFREW-DFSGASLYGEIVERVSKLARENSVYVIFGLLEP---YKSCVYNSALLLDR 99
Query: 408 FGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINIC 548
G+VI KHRK F E + GNT T++GKVAI IC
Sbjct: 100 NGEVILKHRK--------FQEPMKFCTGNTVKTA-RTEFGKVAIIIC 137
>UniRef50_Q46AW4 Cluster: Putative amidohydrolase; n=1;
Methanosarcina barkeri str. Fusaro|Rep: Putative
amidohydrolase - Methanosarcina barkeri (strain Fusaro /
DSM 804)
Length = 287
Score = 41.5 bits (93), Expect = 0.020
Identities = 29/104 (27%), Positives = 48/104 (46%), Gaps = 1/104 (0%)
Frame = +3
Query: 405 EFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYG-KVAINICYGRHHPLNWLM 581
E G + G +RK H + E+ Y+ +G++ P+ K K+ ICY P
Sbjct: 122 ESGTLAGSYRKTHPFKT----ENNYFSKGDSIEPISLKKQNLKIGFEICYDLRFPEVARK 177
Query: 582 FGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
+ G++++ +A + SEH W + A+ AI N A NR
Sbjct: 178 LSLAGSDLLVTTAAFPNPRSEH-WNILAKARAIENQIPHIACNR 220
>UniRef50_A6CFF3 Cluster: Putative nitrilase; n=1; Planctomyces
maris DSM 8797|Rep: Putative nitrilase - Planctomyces
maris DSM 8797
Length = 343
Score = 41.1 bits (92), Expect = 0.026
Identities = 40/168 (23%), Positives = 69/168 (41%), Gaps = 9/168 (5%)
Frame = +3
Query: 93 AISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQP------W 254
A++ +P+ + A EK +I AA ++ E + F + P +
Sbjct: 9 ALAHVSPVFLNKDATVEKSCSLIREAARNGAQMIVFPETYIPAFPVWCALQAPIHNHDLF 68
Query: 255 CDFAEPVLT--GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGK 428
C+ A + GP +AE A + +M + E + IWN +I + G ++
Sbjct: 69 CELAANSIKVDGPELAQIAETARECEMFVSMGFNEGTTVSDGCIWNANALIGDDGNILCH 128
Query: 429 HRKNHIPRVGDFNESTYYFEGN-TGHPVFETKYGKVAINICYGRHHPL 569
HRK V F E + G+ G V T+ G++ + IC +PL
Sbjct: 129 HRK----IVPTFYEKLVWSPGDGAGLEVCATRLGRLGMLICGENTNPL 172
>UniRef50_A4R649 Cluster: Putative uncharacterized protein; n=1;
Magnaporthe grisea|Rep: Putative uncharacterized protein
- Magnaporthe grisea (Rice blast fungus) (Pyricularia
grisea)
Length = 521
Score = 41.1 bits (92), Expect = 0.026
Identities = 28/103 (27%), Positives = 52/103 (50%), Gaps = 7/103 (6%)
Frame = +3
Query: 261 FAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTI-----WNTAVVINEFGKVIG 425
F EPV +G S ++ A+KY+ + E+ D + +N+ +++NE G+ +
Sbjct: 61 FLEPVGSGISALWARTTALKYNCKVAIGYPEKADSSSSFLLQGAFFNSLLMVNENGETLA 120
Query: 426 KHRKNHIPRVGDFNESTYYFEGNTG--HPVFETKYGKVAINIC 548
+RK H+ D+ + + FEG G H V + G+V + +C
Sbjct: 121 NYRKQHL----DYADKGWAFEGAGGFFHDVID-GLGRVTMGVC 158
>UniRef50_P54608 Cluster: UPF0012 hydrolase yhcX; n=12;
Bacteria|Rep: UPF0012 hydrolase yhcX - Bacillus subtilis
Length = 513
Score = 41.1 bits (92), Expect = 0.026
Identities = 44/136 (32%), Positives = 61/136 (44%), Gaps = 5/136 (3%)
Frame = +3
Query: 306 ELAVKYDMVII--SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
+LAVKY++ II S +E + I+N A + G I K K HI NE +
Sbjct: 305 DLAVKYNVNIIGGSHFVEEEG----KIYNIAYLFRRDG-TIEKQYKLHITP----NERKW 355
Query: 480 Y-FEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATV--SGLSEHL 650
+ VF+T GK+AI ICY P + GA+I+F P T G
Sbjct: 356 WGISAGDQVRVFDTDCGKIAIQICYDIEFPELARIAADKGAKIIFTPFCTEDRQGYLRVR 415
Query: 651 WAVEARNAAIANSYYT 698
+ +AR A+ N YT
Sbjct: 416 YCSQAR--AVENQIYT 429
>UniRef50_O66508 Cluster: Putative uncharacterized protein; n=1;
Aquifex aeolicus|Rep: Putative uncharacterized protein -
Aquifex aeolicus
Length = 246
Score = 40.7 bits (91), Expect = 0.035
Identities = 25/58 (43%), Positives = 34/58 (58%)
Frame = +3
Query: 378 IWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICY 551
I NTA +I + G+VIGK K + + F+E Y+ G + VFETK GK I IC+
Sbjct: 85 ILNTAFLIED-GRVIGKRSKIKLFPI--FDEDKYFIPGKE-NKVFETKLGKAGILICF 138
>UniRef50_A4YP30 Cluster: N-carbamoyl-D-amino acid hydrolase; n=4;
Proteobacteria|Rep: N-carbamoyl-D-amino acid hydrolase -
Bradyrhizobium sp. (strain ORS278)
Length = 332
Score = 40.7 bits (91), Expect = 0.035
Identities = 27/88 (30%), Positives = 46/88 (52%), Gaps = 12/88 (13%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHI-----PRVG---DFNESTYYFEGNTGHPVFET--KYGK 530
+N+A++++ G++I K+RK H+ PR G E Y+ G+ G P ++G
Sbjct: 94 FNSAILVDADGQLISKYRKVHLPGSVEPREGARYQQLEKRYFGYGDLGFPAVRAGPEWGG 153
Query: 531 --VAINICYGRHHPLNWLMFGINGAEIV 608
+ + IC R P +W M G+ G E+V
Sbjct: 154 AIMGMMICNDRRWPESWRMLGMQGVELV 181
>UniRef50_Q4P7D2 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 352
Score = 40.7 bits (91), Expect = 0.035
Identities = 34/114 (29%), Positives = 54/114 (47%), Gaps = 6/114 (5%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRV---GDFN--ESTYYFEGNTGHPVFETKYGKVAINI 545
+NT ++I+ G+++ ++RK H+ V G ES +G+ +T +GK+ +
Sbjct: 208 YNTQLLIDHSGEILDRYRKLHLFDVDIKGGLKILESDSTIKGDRLLTPRQTPFGKLGMLT 267
Query: 546 CYGRHHPLNWLMFGINGAEIVFNPSA-TVSGLSEHLWAVEARNAAIANSYYTCA 704
CY P L GA+++ PSA TV + H W V R AI Y A
Sbjct: 268 CYDLRFPEPSLSLRRQGAQVLTYPSAFTVRTGAAH-WEVLLRARAIETQSYVLA 320
>UniRef50_A7I641 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Candidatus
Methanoregula boonei 6A8|Rep: Nitrilase/cyanide
hydratase and apolipoprotein N-acyltransferase -
Methanoregula boonei (strain 6A8)
Length = 265
Score = 40.7 bits (91), Expect = 0.035
Identities = 26/109 (23%), Positives = 48/109 (44%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHH 563
NTA+ I+ G ++ + K H+ G +++ F TG F + ++ + ICY
Sbjct: 91 NTAIAIDRNGTILTTYAKIHLFTPGREDQA---FSPGTGLATFALEGVQIGLAICYDLRF 147
Query: 564 PLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAIN 710
P + ++ G V P+A +H W + ++ A N Y +N
Sbjct: 148 PEIFRLYRQRGVHAVIVPAAWPKSRLKH-WELFIQSRAAENQMYIAGVN 195
>UniRef50_Q6RWR2 Cluster: Nitrilase; n=1; uncultured organism|Rep:
Nitrilase - uncultured organism
Length = 336
Score = 40.3 bits (90), Expect = 0.046
Identities = 35/123 (28%), Positives = 54/123 (43%), Gaps = 3/123 (2%)
Frame = +3
Query: 261 FAEPVLT--GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHR 434
FAE +T GP T +A ++M + + ER G T++NT + G ++G+HR
Sbjct: 69 FAEQAITIPGPETECIAAACRAHNMTVAIGVTERPARAG-TLYNTLLYFGPDGMILGRHR 127
Query: 435 KNHIPRVGDFNESTYYFEGN-TGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVF 611
K + FNE + G+ T ET V IC+ PL + G +I
Sbjct: 128 K----LMPTFNERMVWGMGDGTTLRTIETPQAVVGGLICWENFMPLARTVLYTQGEQIHV 183
Query: 612 NPS 620
P+
Sbjct: 184 APT 186
>UniRef50_Q8KFB2 Cluster: Carbon-nitrogen hydrolase family protein;
n=3; Chlorobiaceae|Rep: Carbon-nitrogen hydrolase family
protein - Chlorobium tepidum
Length = 519
Score = 40.3 bits (90), Expect = 0.046
Identities = 38/137 (27%), Positives = 63/137 (45%), Gaps = 3/137 (2%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
AEPV GPS +AE+A I+ E D G +N+A V+ + GK++ +RK
Sbjct: 62 AEPV-DGPSVQAMAEIAEAAGCYIVLGYPEIDPCTG-ICYNSAAVLGQDGKLVLNYRKVT 119
Query: 444 IPRVGDFNESTYYFEGN-TGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPS 620
E+ + G+ +FET +G+ A+ IC ++ L + GA+++ P+
Sbjct: 120 A-------EARWACPGSHMQESLFETPWGRAAVLICSDSYYGLIPRAAALRGADLLLVPA 172
Query: 621 ATVSGLSE--HLWAVEA 665
G + LW A
Sbjct: 173 NWPGGSLDPRELWRARA 189
>UniRef50_Q89XU5 Cluster: Amidohydrolase; n=48;
Alphaproteobacteria|Rep: Amidohydrolase - Bradyrhizobium
japonicum
Length = 292
Score = 40.3 bits (90), Expect = 0.046
Identities = 30/112 (26%), Positives = 45/112 (40%), Gaps = 5/112 (4%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINIC 548
N + +I G V+ + K H +P + ES Y G T + + +G+V + IC
Sbjct: 101 NRSFLIGPEGNVLASYDKIHMFDIELPDGESYRESANYQPGETA-VISDLPWGRVGLTIC 159
Query: 549 YGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
Y P + +GA + PSA E W V R AI + A
Sbjct: 160 YDVRFPALYRALAESGAYFITVPSAFTRKTGEAHWHVLLRARAIETGCFVFA 211
>UniRef50_Q4KB18 Cluster: Hydrolase, carbon-nitrogen family; n=2;
Bacteria|Rep: Hydrolase, carbon-nitrogen family -
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Length = 325
Score = 39.9 bits (89), Expect = 0.061
Identities = 26/91 (28%), Positives = 42/91 (46%), Gaps = 1/91 (1%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
L E A + ++ + ER HG +++N+ V I G ++ HRK E T
Sbjct: 95 LREAARVNSVTVVMGMNERSRRHGGSLYNSLVTIGPEGTILNVHRK----LTPTHTERTV 150
Query: 480 YFEGN-TGHPVFETKYGKVAINICYGRHHPL 569
+ G+ G V +T G+V +C+ HPL
Sbjct: 151 WANGDAAGLRVVDTAVGRVGGLVCWEHWHPL 181
>UniRef50_A4XAH8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2; Salinispora|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Salinispora tropica CNB-440
Length = 270
Score = 39.9 bits (89), Expect = 0.061
Identities = 43/154 (27%), Positives = 66/154 (42%), Gaps = 7/154 (4%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDM-VIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKN 440
AEPV G F A+ A + + V++ I ER + +NT +V + G + +RK
Sbjct: 54 AEPV-DGEVGRFFADAAQRLGVWVVVGSIHERGP-DPEHSYNTCLVFDRSGTLAASYRKI 111
Query: 441 HIPRVGDFNESTYYFEGNT----GHPVFETKYG-KVAINICYGRHHP-LNWLMFGINGAE 602
H+ V + Y E T PV G +V ++ICY P L + GA+
Sbjct: 112 HLYDV-EIPGRVSYLESATVAAGAQPVVVDVEGIRVGLSICYDLRFPELYRQLVTDGGAD 170
Query: 603 IVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
++ P+A + W V R AI N + A
Sbjct: 171 LLLVPAAFMLHTGRDHWEVLLRARAIENQCFVAA 204
>UniRef50_A0J1U1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Shewanella woodyi
ATCC 51908|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Shewanella woodyi
ATCC 51908
Length = 288
Score = 39.9 bits (89), Expect = 0.061
Identities = 25/111 (22%), Positives = 54/111 (48%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRV 455
+ G T L ++A + + +++ + E D G++ ++T+ +I+ G +IGK+R+ H
Sbjct: 64 IPGECTDKLCQIAKEGGIYLVAGLFEVD---GESYFSTSFLISPTGNIIGKYRRVHC--- 117
Query: 456 GDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIV 608
F Y + PVF T G++ + Y + P++ + +I+
Sbjct: 118 --FEMERKYISQGSDFPVFNTDIGRIGLLQGYDINFPISCMELYCKEVDII 166
>UniRef50_Q1AZG5 Cluster: Nitrilase; n=1; Rubrobacter xylanophilus
DSM 9941|Rep: Nitrilase - Rubrobacter xylanophilus
(strain DSM 9941 / NBRC 16129)
Length = 359
Score = 39.5 bits (88), Expect = 0.080
Identities = 36/162 (22%), Positives = 76/162 (46%), Gaps = 8/162 (4%)
Frame = +3
Query: 108 NPITQQRLAIFEKVQKIISAAAAEQVNILCLQEA-------WNMPFAFCTREKQPWCDFA 266
+P+ + A +K++ +++ AA ++ E+ WN+ + F
Sbjct: 17 SPVHLKPDATVDKLESLVAEAARGGAQLVVFSESFIPAFPVWNLVLPPVDQHDLFRRLFL 76
Query: 267 EPVLT-GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
VL GP T LAE+A ++D+ + + ER +I ++NT ++ G+++ HR+
Sbjct: 77 NSVLVPGPITRRLAEIAKRHDVYLSVGVTERTNISMGCLYNTNLLFAPTGELL-NHRRKL 135
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPL 569
+P + + + + + PV +T+ G + + IC +PL
Sbjct: 136 VPTWAE-KLTHAWGDASDLRPV-QTELGNIGVLICGENTNPL 175
>UniRef50_A5UTD2 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase precursor; n=2;
Roseiflexus|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase precursor - Roseiflexus
sp. RS-1
Length = 509
Score = 39.5 bits (88), Expect = 0.080
Identities = 23/78 (29%), Positives = 36/78 (46%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHH 563
+ AV+ G +G+H K ++ GD ++ + G VF T YG V + +C+ RH
Sbjct: 331 DAAVLFGPDGNEVGRHAKINL--TGD-EQAFGFVPGPRDFQVFTTPYGNVGLGVCWDRHV 387
Query: 564 PLNWLMFGINGAEIVFNP 617
P GA +V P
Sbjct: 388 PWITRELARAGAHVVLMP 405
>UniRef50_Q8KCC8 Cluster: Carbon-nitrogen hydrolase family protein;
n=10; Chlorobiaceae|Rep: Carbon-nitrogen hydrolase
family protein - Chlorobium tepidum
Length = 286
Score = 39.1 bits (87), Expect = 0.11
Identities = 35/139 (25%), Positives = 56/139 (40%), Gaps = 9/139 (6%)
Frame = +3
Query: 324 DMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGH 503
D+ I +E D +G ++N+A + + G HRK ++P G F E Y+ G
Sbjct: 77 DICIFCGGIELSDDYG--VYNSAFMFED-GAGRSVHRKIYLPTYGMFEELRYFSAGRQIE 133
Query: 504 PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIV---------FNPSATVSGLSEHLWA 656
V + GKV + IC H + GA+++ +P V + W
Sbjct: 134 TVTSRRIGKVGVAICEDFWHMSVPYLLAHQGAKLLLVLMSSPLRLSPGQGVPAIVTQ-WQ 192
Query: 657 VEARNAAIANSYYTCAINR 713
A +A S Y +NR
Sbjct: 193 TIASTSAFLLSCYVACVNR 211
>UniRef50_Q16A64 Cluster: Hydrolase, putative; n=1; Roseobacter
denitrificans OCh 114|Rep: Hydrolase, putative -
Roseobacter denitrificans (strain ATCC 33942 / OCh 114)
(Erythrobactersp. (strain OCh 114)) (Roseobacter
denitrificans)
Length = 261
Score = 39.1 bits (87), Expect = 0.11
Identities = 23/82 (28%), Positives = 38/82 (46%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHH 563
N VVI+ G + ++ K H+ GD + + + G VF+ KV + ICY
Sbjct: 92 NACVVIDNTGTQVARYHKTHL--FGDVDRAQFS-AGAALSEVFDLAGWKVGLAICYDVEF 148
Query: 564 PLNWLMFGINGAEIVFNPSATV 629
P + GAE++ P+A +
Sbjct: 149 PELIRSLALRGAEVILTPTANM 170
>UniRef50_A5V962 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Sphingomonas
wittichii RW1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Sphingomonas
wittichii RW1
Length = 268
Score = 39.1 bits (87), Expect = 0.11
Identities = 33/117 (28%), Positives = 49/117 (41%), Gaps = 5/117 (4%)
Frame = +3
Query: 369 GDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFEGNTGHPVFETKYGKV 533
GD NT++V + G+ IG++ K H +P ES G+ V + + KV
Sbjct: 88 GDRFLNTSLVFDRQGECIGRYSKLHRFDIDLPDGTAIRESDVVDRGD-AITVVDIEGLKV 146
Query: 534 AINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
A+ ICY P + GA+++ P+A W V R AI Y A
Sbjct: 147 ALTICYDLRFPELFRALVDLGADLITVPAAFTFQTGADHWEVLLRARAIETECYIAA 203
>UniRef50_A2ICY3 Cluster: Cyanide hydratase; n=23;
Gammaproteobacteria|Rep: Cyanide hydratase - Pseudomonas
aeruginosa
Length = 282
Score = 39.1 bits (87), Expect = 0.11
Identities = 30/111 (27%), Positives = 49/111 (44%), Gaps = 6/111 (5%)
Frame = +3
Query: 390 AVVINEFGKVIGKHRKNHIPRV------GDFNESTYYFEGNTGHPVFETKYGKVAINICY 551
+++I+E G+ + ++ K H+ V G + ES Y G V +T G++ + +CY
Sbjct: 100 SLLIDEHGERVARYDKLHLFDVDVADARGRYRESDDYAFGQK-IVVADTPVGRLGLTVCY 158
Query: 552 GRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
P + GAE++ PSA + W V R AI Y A
Sbjct: 159 DLRFPELYTALREAGAELITAPSAFTAVTGAAHWQVLVRARAIETQCYLLA 209
>UniRef50_UPI0000E105FE Cluster: putative hydrolase, carbon-nitrogen
family protein; n=1; alpha proteobacterium HTCC2255|Rep:
putative hydrolase, carbon-nitrogen family protein -
alpha proteobacterium HTCC2255
Length = 279
Score = 38.7 bits (86), Expect = 0.14
Identities = 33/144 (22%), Positives = 63/144 (43%), Gaps = 6/144 (4%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
L+++A Y + +++ + + ++ TA + G+++ ++ K H+ V + +
Sbjct: 77 LSDIAKTYHIWLVAGSIPTPSPDPNKMFATAWCFDPSGELVAQYNKTHLFDVSITDNTGT 136
Query: 480 YFEGNTGHP-----VFETKYGKVAINICYG-RHHPLNWLMFGINGAEIVFNPSATVSGLS 641
Y E T P V +T++G+V I ICY R L M N + + P+A
Sbjct: 137 YQESATTMPGSDVVVLDTEFGRVGICICYDIRFSTLFNAMVKENAIDYLVVPAAFTYQTG 196
Query: 642 EHLWAVEARNAAIANSYYTCAINR 713
+ W + AI Y A N+
Sbjct: 197 QAHWHHLLASRAIEYQCYVIAANQ 220
>UniRef50_Q7URE5 Cluster: Predicted amidohydrolase; n=1; Pirellula
sp.|Rep: Predicted amidohydrolase - Rhodopirellula
baltica
Length = 314
Score = 38.7 bits (86), Expect = 0.14
Identities = 31/116 (26%), Positives = 52/116 (44%), Gaps = 1/116 (0%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
A P + P+ L E + I L R D D + N+A++I+ G ++G++ K H
Sbjct: 87 AAPTIDSPAIGRLIEACQANRLTITIGTLIRKD--RDELHNSALMIDGSG-LLGRYNKVH 143
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYG-KVAINICYGRHHPLNWLMFGINGAEIV 608
+P +G + G F T+ G V + ICY P G+ GA+++
Sbjct: 144 LPHLG---VDRFVDRGLFCDQTFTTQSGCNVGLGICYDSSFPEPMRALGLAGADVI 196
>UniRef50_A3M2Z7 Cluster: Putative glutamine-dependent NAD(+)
synthetase (NAD(+) synthase); n=1; Acinetobacter
baumannii ATCC 17978|Rep: Putative glutamine-dependent
NAD(+) synthetase (NAD(+) synthase) - Acinetobacter
baumannii (strain ATCC 17978 / NCDC KC 755)
Length = 364
Score = 38.7 bits (86), Expect = 0.14
Identities = 25/82 (30%), Positives = 47/82 (57%)
Frame = +3
Query: 303 AELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYY 482
A+L+ D+V++ + + + G +N+A V+ + G+V+G K+++P G F+E Y+
Sbjct: 46 AQLSEVKDIVMVFGFVNQTE-DGQR-YNSAAVMKD-GQVLGVFNKHNLPNYGVFDEKRYF 102
Query: 483 FEGNTGHPVFETKYGKVAINIC 548
+G+ H VFE K + IC
Sbjct: 103 QKGHQ-HLVFEYLGHKFGVLIC 123
>UniRef50_Q7QAW0 Cluster: ENSANGP00000011026; n=2; Culicidae|Rep:
ENSANGP00000011026 - Anopheles gambiae str. PEST
Length = 278
Score = 38.7 bits (86), Expect = 0.14
Identities = 42/189 (22%), Positives = 78/189 (41%), Gaps = 5/189 (2%)
Frame = +3
Query: 159 ISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVII 338
I A ++ L E +N P++ T E + AE + G ++ LA++A + + ++
Sbjct: 30 IRQAKDRGARLIILPECFNSPYS--TAE---FGRHAEEIPRGETSQALAKVAAELGVYLV 84
Query: 339 SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFEGNTGH 503
+ G ++NT V G+++ K+RK H IP F ES G+
Sbjct: 85 GGTYPERE--GTRLYNTCPVFGPKGELLCKYRKLHLFDMDIPGRCTFQESAALTAGDR-L 141
Query: 504 PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIA 683
F K+ + IC+ + P + G +++ PSA W + R A+
Sbjct: 142 ATFSIGSLKIGLGICWDKRFPELAACYRQLGCDMMIFPSAFDPYTGPLHWDLLGRARALD 201
Query: 684 NSYYTCAIN 710
N + ++
Sbjct: 202 NQMFVALVS 210
>UniRef50_Q6RWE5 Cluster: Nitrilase; n=4; root|Rep: Nitrilase -
uncultured organism
Length = 332
Score = 38.3 bits (85), Expect = 0.19
Identities = 38/131 (29%), Positives = 60/131 (45%), Gaps = 4/131 (3%)
Frame = +3
Query: 303 AELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYY 482
AEL V + + ILE T++N+ ++I+E GK+ G HRK V + E +
Sbjct: 95 AELGVVISIGVNEKILEGPG--NGTLYNSLLLIDESGKLAGHHRK----LVPTYTERMVW 148
Query: 483 FEGN-TGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEI---VFNPSATVSGLSEHL 650
G+ G T G+V IC+ PL+ + ++G EI V+ V L+
Sbjct: 149 GMGDGGGMEAISTAAGRVGGLICWEHWMPLSRQVLHMSGEEIHVAVWPTVHEVHQLASRH 208
Query: 651 WAVEARNAAIA 683
+A E R +A
Sbjct: 209 YAFEGRCFVLA 219
>UniRef50_Q8Y8V0 Cluster: Lmo0792 protein; n=12; Listeria|Rep:
Lmo0792 protein - Listeria monocytogenes
Length = 296
Score = 38.3 bits (85), Expect = 0.19
Identities = 39/143 (27%), Positives = 66/143 (46%), Gaps = 2/143 (1%)
Frame = +3
Query: 201 QEAWNMPFAF-CTREKQPWCDFAEPVLTGPSTVF-LAELAVKYDMVIISPILERDDIHGD 374
++A+N P A E+ W D E + + V L +LA + + I + L + + +
Sbjct: 55 EDAFNHPLATGFGAERFKWLD--EAIAADSAYVSTLKKLAKELQIGICATYLSKTEQNSQ 112
Query: 375 TIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYG 554
NTA++I+ G++I + K H DF+ G V E K+ + ICY
Sbjct: 113 ---NTAIIIDRKGEIILDYAKVH---TCDFSLEILLQSGEE-FKVCEFDGIKLGVMICYD 165
Query: 555 RHHPLNWLMFGINGAEIVFNPSA 623
R P + + + GAEI+ P+A
Sbjct: 166 REFPESARILMLKGAEIILVPNA 188
>UniRef50_Q2BKP4 Cluster: Putative carbon-nitrogen hydrolase; n=1;
Neptuniibacter caesariensis|Rep: Putative
carbon-nitrogen hydrolase - Neptuniibacter caesariensis
Length = 276
Score = 38.3 bits (85), Expect = 0.19
Identities = 25/84 (29%), Positives = 36/84 (42%)
Frame = +3
Query: 462 FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLS 641
+ ES Y+ G V +T G ++ICY P ++ GA I+ PSA +
Sbjct: 132 YRESDYFTPGKE-LVVEQTSVGCFGLSICYDLRFPEHYQRLADMGANIMLVPSAFTAVTG 190
Query: 642 EHLWAVEARNAAIANSYYTCAINR 713
+ W V R AI Y A N+
Sbjct: 191 KAHWEVLLRARAIETQSYVIAANQ 214
>UniRef50_Q12DE7 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=6;
Proteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Polaromonas sp.
(strain JS666 / ATCC BAA-500)
Length = 321
Score = 38.3 bits (85), Expect = 0.19
Identities = 21/83 (25%), Positives = 39/83 (46%), Gaps = 7/83 (8%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRVGDFNE-------STYYFEGNTGHPVFETKYGKVAI 539
+NT+++++ FG+++ K+RK H+P + YF G V G + +
Sbjct: 109 YNTSILVDRFGQIVAKYRKVHLPGHKEHEPWRRFQHLEKRYFTPGPGFGVTNAFGGVMGM 168
Query: 540 NICYGRHHPLNWLMFGINGAEIV 608
IC R + + G+ G E+V
Sbjct: 169 AICNDRRWAETYRVMGLQGVEMV 191
>UniRef50_A6T2L9 Cluster: Nitrilase; n=1; Janthinobacterium sp.
Marseille|Rep: Nitrilase - Janthinobacterium sp. (strain
Marseille) (Minibacterium massiliensis)
Length = 355
Score = 38.3 bits (85), Expect = 0.19
Identities = 22/92 (23%), Positives = 44/92 (47%), Gaps = 1/92 (1%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRV 455
+ GP L E A ++ + + I E I +W+T ++I + G ++ +HRK +
Sbjct: 79 INGPEVAQLREAARRHGVFVSMGINEGTPISMGCVWDTNILIGDDGSILNRHRK----LI 134
Query: 456 GDFNESTYYFEGN-TGHPVFETKYGKVAINIC 548
E + G+ +G V +T+ G++ +C
Sbjct: 135 ATHWEKLAWASGDGSGLRVVDTRIGRIGALVC 166
>UniRef50_A4U2A6 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=3;
Magnetospirillum|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Magnetospirillum
gryphiswaldense
Length = 279
Score = 38.3 bits (85), Expect = 0.19
Identities = 28/119 (23%), Positives = 51/119 (42%), Gaps = 5/119 (4%)
Frame = +3
Query: 363 IHGDTIWNTAVVINEFGKVIGKHRKNHIPRVG-----DFNESTYYFEGNTGHPVFETKYG 527
+ G + N + VI++ G ++G++ K H+ V + ES + G+ V +G
Sbjct: 94 LDGGMVANRSYVIDKNGLILGRYDKIHMFDVDLGGGESYRESATFTPGDRATMV-RLPWG 152
Query: 528 KVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
++ +++CY P + + GA + P+A W V R AI Y A
Sbjct: 153 RLGLSVCYDLRFPHLYRAYANAGAHFLAVPAAFTRTTGRAHWHVLLRARAIETGCYVFA 211
>UniRef50_Q74FF8 Cluster: Hydrolase, carbon-nitrogen family; n=6;
Geobacter|Rep: Hydrolase, carbon-nitrogen family -
Geobacter sulfurreducens
Length = 283
Score = 37.9 bits (84), Expect = 0.25
Identities = 26/89 (29%), Positives = 47/89 (52%), Gaps = 1/89 (1%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRH 560
+NT++ + E G+V HRK ++P G F+E Y G F++++G++ + IC
Sbjct: 93 FNTSLYL-EGGEVRHVHRKVYLPTYGLFDEQRYLARGE-HFRAFDSRFGRMGLLICEDMW 150
Query: 561 HPLNWLMFGINGAEIVFNPSATVS-GLSE 644
H + ++GA V S++ GL+E
Sbjct: 151 HLSAPYILAMDGATTVICLSSSPGRGLTE 179
>UniRef50_Q0VS65 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Alcanivorax borkumensis SK2|Rep: Carbon-nitrogen
hydrolase family protein - Alcanivorax borkumensis
(strain SK2 / ATCC 700651 / DSM 11573)
Length = 285
Score = 37.9 bits (84), Expect = 0.25
Identities = 36/153 (23%), Positives = 62/153 (40%), Gaps = 14/153 (9%)
Frame = +3
Query: 297 FLAELAVKYDMVIIS---PILERDD---IHGDTIWNTAVVINEFGKVIGKHRKNHI---- 446
+L E A + M II P L R D + + ++ ++ G+V+G++ K H+
Sbjct: 69 WLCEQASRLGMAIIGGSIPSLTRPDGEPVPAPRVRTRSLAVSSEGQVVGRYDKLHLFDAQ 128
Query: 447 --PRVGDFNESTYYFEGNTGHPVFETKYG--KVAINICYGRHHPLNWLMFGINGAEIVFN 614
G + ES ++ G + G +V + ICY P GAE++
Sbjct: 129 VHDAQGQYRESDFF---EPGEAIVTAPLGGVQVGLAICYDLRFPALAQRLTSAGAELLVY 185
Query: 615 PSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
PSA + + W + R A+ Y N+
Sbjct: 186 PSAFTAVTGKAHWELLLRATAVQTGCYVLGANQ 218
>UniRef50_A3JK79 Cluster: Predicted amidohydrolase; n=3;
Gammaproteobacteria|Rep: Predicted amidohydrolase -
Marinobacter sp. ELB17
Length = 280
Score = 37.9 bits (84), Expect = 0.25
Identities = 37/147 (25%), Positives = 60/147 (40%), Gaps = 11/147 (7%)
Frame = +3
Query: 297 FLAELAVKYDMVIIS---PILERDD--IHGDTIWNTAVVINEFGKVIGKHRKNHI----- 446
FLA+ A + I+ P+ R D + D + + +V N+ G + ++ K H+
Sbjct: 73 FLAQQAKTLKIWIVGGSLPLALRPDGSVMADRVRASCLVFNDLGDEVARYDKIHLFDAQV 132
Query: 447 -PRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
G + ES + G+ V +T G++ + +CY P + GA+ V PSA
Sbjct: 133 DDAHGQYRESDTFEAGDQVVTV-DTPAGRLGLAVCYDLRFPELFRALRDKGADWVCLPSA 191
Query: 624 TVSGLSEHLWAVEARNAAIANSYYTCA 704
W R AI N Y A
Sbjct: 192 FTWKTGNAHWHALIRARAIENQLYVVA 218
>UniRef50_A2R283 Cluster: Contig An13c0120, complete genome; n=2;
Aspergillus|Rep: Contig An13c0120, complete genome -
Aspergillus niger
Length = 598
Score = 37.9 bits (84), Expect = 0.25
Identities = 39/154 (25%), Positives = 61/154 (39%), Gaps = 12/154 (7%)
Frame = +3
Query: 240 EKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKV 419
E + W + + +LT P T L + A + II E D GD + G +
Sbjct: 64 ELEDWFEHGD-ILTAPRTKALFDTAHDLAVDIIVGFAEATDT-GDHFNSCVYYHAATGSI 121
Query: 420 IGKHRKNHI---------PRVGDFNESTYYFEGNTGHPVFETK---YGKVAINICYGRHH 563
+ K+RK H+ P+ + E Y+ G+ G F K + + IC R
Sbjct: 122 LSKYRKVHLPGDVEPLPDPKAVNQLEKRYFKPGDLGFQAFREKDVVDPILGMMICNDRRW 181
Query: 564 PLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEA 665
+W +G+ G EIV T +G + W A
Sbjct: 182 AESWREYGLQGVEIVACGYNT-NGFAPQFWGQSA 214
>UniRef50_A1CIE7 Cluster: Hydrolase, carbon-nitrogen family protein;
n=8; Pezizomycotina|Rep: Hydrolase, carbon-nitrogen
family protein - Aspergillus clavatus
Length = 260
Score = 37.9 bits (84), Expect = 0.25
Identities = 35/114 (30%), Positives = 51/114 (44%), Gaps = 7/114 (6%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNHI--PRVGD---FNESTYYFEGNTGHPVFETKYGKVAINIC 548
NT + I+ G + +++K H+ + D ES +G P FET G+V + IC
Sbjct: 81 NTLIWIDNKGVITQRYQKIHLFDVEIKDGPILKESASVEKGTDILPPFETPLGRVGLAIC 140
Query: 549 YGRHHPLNWLMFGINGAEIVFNPSA-TV-SGLSEHLWAVEARNAAIANSYYTCA 704
+ P L A+I+ PSA TV +GL+ W R AI Y A
Sbjct: 141 FDLRFPEISLALKRQNAQIITYPSAFTVPTGLAH--WETLIRARAIETQSYVVA 192
>UniRef50_UPI0000DAE70E Cluster: hypothetical protein
Rgryl_01001070; n=1; Rickettsiella grylli|Rep:
hypothetical protein Rgryl_01001070 - Rickettsiella
grylli
Length = 543
Score = 37.5 bits (83), Expect = 0.32
Identities = 21/65 (32%), Positives = 32/65 (49%)
Frame = +3
Query: 414 KVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGIN 593
K++ + K ++P G F+E Y F+ T H +F K V I IC H L N
Sbjct: 104 KIVNTYHKQYLPNYGVFDECRY-FKSGTQHGLFNIKGLPVGILICEDLWHSQPALTHKEN 162
Query: 594 GAEIV 608
GA+++
Sbjct: 163 GAKLL 167
>UniRef50_UPI000023E628 Cluster: hypothetical protein FG00821.1;
n=1; Gibberella zeae PH-1|Rep: hypothetical protein
FG00821.1 - Gibberella zeae PH-1
Length = 305
Score = 37.5 bits (83), Expect = 0.32
Identities = 37/149 (24%), Positives = 60/149 (40%), Gaps = 7/149 (4%)
Frame = +3
Query: 141 EKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVK 320
E+ K++++AA Q +L L EA + + + + AEP T L E A +
Sbjct: 20 EQCVKLVASAARGQAKVLFLPEAAD----YIASNGKESLELAEPQSTSSFVSGLREAARE 75
Query: 321 YDMVIISPILERDDI-----HGDTIWNTAVVINEFGKV--IGKHRKNHIPRVGDFNESTY 479
+ + + I RD+ I N + IN G++ + K H G ES
Sbjct: 76 HRVAVHVGIHHRDETDIGQEQSKRILNRTIYINADGQIDDTATYDKLHAFDFGKMKESDT 135
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHP 566
G T F+T G++ IC+ P
Sbjct: 136 VQPGKTLTAPFDTPIGRIGSLICFDLRFP 164
>UniRef50_Q2RGR0 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Moorella
thermoacetica ATCC 39073|Rep: Nitrilase/cyanide
hydratase and apolipoprotein N-acyltransferase -
Moorella thermoacetica (strain ATCC 39073)
Length = 245
Score = 37.5 bits (83), Expect = 0.32
Identities = 33/125 (26%), Positives = 53/125 (42%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
LA +A + + + I+ R + G+ ++N+A V G V +RK ++ +
Sbjct: 64 LARIARRAADLGVGLIVGRAEFAGERLFNSASVFLPDGSV-HTYRKIYLT-----DAEAR 117
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAV 659
YF TGH VF K K + IC +++P GA +F SA E W +
Sbjct: 118 YFTPGTGHLVFNYKGSKFGVIICRDQNYPELARQIAAEGARALFILSAHYYQPGEARWKL 177
Query: 660 EARNA 674
A
Sbjct: 178 PKNRA 182
>UniRef50_Q1MFH8 Cluster: Putative hydrolase; n=1; Rhizobium
leguminosarum bv. viciae 3841|Rep: Putative hydrolase -
Rhizobium leguminosarum bv. viciae (strain 3841)
Length = 252
Score = 37.5 bits (83), Expect = 0.32
Identities = 27/93 (29%), Positives = 44/93 (47%)
Frame = +3
Query: 330 VIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPV 509
V++ ++E DD ++NTAVV+ E G ++G++RK H+ FE P+
Sbjct: 82 VLVMGLIEIDD---GRLFNTAVVV-ERGVLLGRYRKTHLL------PGERAFEAGKDSPL 131
Query: 510 FETKYGKVAINICYGRHHPLNWLMFGINGAEIV 608
F + INICY + P +GA +
Sbjct: 132 FAIGALRFGINICYDTNFPEAAAKVAASGASAI 164
>UniRef50_A1IFV0 Cluster: Putative hydrolase; n=1; Candidatus
Desulfococcus oleovorans Hxd3|Rep: Putative hydrolase -
Candidatus Desulfococcus oleovorans Hxd3
Length = 272
Score = 37.5 bits (83), Expect = 0.32
Identities = 35/141 (24%), Positives = 57/141 (40%), Gaps = 3/141 (2%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
L+ +A +D+VI++ + + G + +VI+ G G + K HI E
Sbjct: 71 LSRMATAFDIVILAGTVA--EAAGGRVTACHLVISPNGSA-GGYTKLHIAPP----EKQL 123
Query: 480 YFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEH---L 650
+ G P+FE K + +CY H P + GA+I+F P A+ E
Sbjct: 124 FVPGRKV-PLFEAKGAVFGVQLCYDAHFPELSTAMALKGADILFVPHASPRNTPEEKLAS 182
Query: 651 WAVEARNAAIANSYYTCAINR 713
W A N + A N+
Sbjct: 183 WMRHLPARAYDNGVFVAACNQ 203
>UniRef50_A6SN02 Cluster: Nitrilase; n=3; Sclerotiniaceae|Rep:
Nitrilase - Botryotinia fuckeliana B05.10
Length = 1187
Score = 37.5 bits (83), Expect = 0.32
Identities = 33/108 (30%), Positives = 50/108 (46%), Gaps = 4/108 (3%)
Frame = +3
Query: 363 IHGDT--IWNTAVVINEFGKVIGKHRKNHI--PRVGDFNESTYYFEGNTGHPVFETKYGK 530
+H +T + NTA I+ GK++ + K ++ P ST N H F+T GK
Sbjct: 109 LHPETSHLHNTAHFISPEGKIVSSYNKKNLWHPERPHLTSST-----NDAHTTFDTPLGK 163
Query: 531 VAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNA 674
V + IC+ P + GA+I+ P T LS+ A ARN+
Sbjct: 164 VGMLICWDAAFPEAFRELVSQGAKIIIIP--TFWTLSDCTPAGLARNS 209
>UniRef50_A3HXT3 Cluster: Putative nitrilase; n=1; Algoriphagus sp.
PR1|Rep: Putative nitrilase - Algoriphagus sp. PR1
Length = 305
Score = 37.1 bits (82), Expect = 0.43
Identities = 37/139 (26%), Positives = 64/139 (46%), Gaps = 1/139 (0%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTY 479
L +L + ++ ++ + ER HG +++ + + I+ G ++G HRK I G E
Sbjct: 88 LEKLCHQLNIYLVCGVTERMKQHG-SLYCSMIYISPKG-LLGVHRK--IKPTGI--ERLV 141
Query: 480 YFEGNTGHPV-FETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWA 656
+ E + V F+TK GK+ IC+ + PL + G EI P+A + W
Sbjct: 142 WAEASGDSLVTFDTKIGKLGGLICWENYMPLARMAMYSQGVEIYIAPTAD----ARESWV 197
Query: 657 VEARNAAIANSYYTCAINR 713
R+ AI + A N+
Sbjct: 198 DTMRHIAIEGRCFVLACNQ 216
>UniRef50_A0NZI0 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Rhodobacteraceae|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Stappia aggregata IAM
12614
Length = 258
Score = 37.1 bits (82), Expect = 0.43
Identities = 23/84 (27%), Positives = 39/84 (46%)
Frame = +3
Query: 372 DTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICY 551
+ ++N A G+ +G +RK I G+ ++++ F T + VFE K A+ ICY
Sbjct: 89 EAVYNAATAFGTSGEQLGHYRK--IQLFGEMEKASFNF--GTQYTVFEFGGRKTALLICY 144
Query: 552 GRHHPLNWLMFGINGAEIVFNPSA 623
P + G +V P+A
Sbjct: 145 DVEFPQHCRRLAEQGVSLVLVPTA 168
>UniRef50_A3LY98 Cluster: Nitrilase superfamily member; n=3;
Saccharomycetaceae|Rep: Nitrilase superfamily member -
Pichia stipitis (Yeast)
Length = 309
Score = 37.1 bits (82), Expect = 0.43
Identities = 52/199 (26%), Positives = 82/199 (41%), Gaps = 13/199 (6%)
Frame = +3
Query: 147 VQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYD 326
V K+I A +QV++L L EA + + +R Q + A T + F++ + +
Sbjct: 27 VNKLIQQAVQKQVSVLFLPEATD----YLSRNAQHSYELA----TSTHSKFVSVIQKQLQ 78
Query: 327 MVIISPILERDDIH-----GDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFN-----EST 476
+ +S IH G + N + ++ GK+I +++K H+ V N ES
Sbjct: 79 SLNLSDFYVAIGIHEPTEGGKRVQNNQLWLDAQGKIISRYQKIHLFDVNIKNGPILQESK 138
Query: 477 YYFEGNTG-HPV-FETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHL 650
GN P+ V + ICY P L GA I+ PSA + E
Sbjct: 139 SVEPGNKILEPLAIANSDFSVGLAICYDIRFPELALRLRKLGASIITYPSAFTTKTGEAH 198
Query: 651 WAVEARNAAI-ANSYYTCA 704
W + R A+ A SY A
Sbjct: 199 WELLGRARAVDAQSYVVMA 217
>UniRef50_Q92DM8 Cluster: Lin0785 protein; n=5; Bacteria|Rep:
Lin0785 protein - Listeria innocua
Length = 296
Score = 36.7 bits (81), Expect = 0.57
Identities = 25/80 (31%), Positives = 41/80 (51%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHH 563
NTA++I+ G++I + K H DF+ G+ + V E K+ + ICY R
Sbjct: 113 NTAIIIDRNGEIILDYAKVH---TCDFSLEALLQSGDEFN-VCEFDGIKLGVMICYDREF 168
Query: 564 PLNWLMFGINGAEIVFNPSA 623
P + + + GAEI+ P+A
Sbjct: 169 PESARVLMLKGAEIILVPNA 188
>UniRef50_Q8KFP8 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Chlorobaculum tepidum|Rep: Carbon-nitrogen
hydrolase family protein - Chlorobium tepidum
Length = 271
Score = 36.7 bits (81), Expect = 0.57
Identities = 51/204 (25%), Positives = 83/204 (40%), Gaps = 3/204 (1%)
Frame = +3
Query: 111 PITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPS 290
P +R A E ++ ++ A+ I+ L E + + F +RE+ FAE G +
Sbjct: 11 PRLGERQANLEAIRSLLDPVEAD---IVVLPELCSSGYFFTSREEL--APFAESP-GGVA 64
Query: 291 TVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNE 470
F LA +II+ + E +N+ V +RK+H+ F +
Sbjct: 65 CSFFQGLADAKRAIIIAGMPETAQ---GCFYNSVFVFRPGVADPLVYRKSHL-----FYK 116
Query: 471 STYYFE-GNTGHPVFETKYGKVAINI--CYGRHHPLNWLMFGINGAEIVFNPSATVSGLS 641
+ FE G+TG PV + ++I I CY P + + GA+++ PS V+
Sbjct: 117 ERFVFEPGDTGFPVIRDEQLDISIGIMLCYDWRFPEVSRVLALGGADLIACPSNLVT--- 173
Query: 642 EHLWAVEARNAAIANSYYTCAINR 713
W AI N Y NR
Sbjct: 174 -DAWRKVMPARAIENKLYVAVANR 196
>UniRef50_Q1FPL1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Clostridium phytofermentans ISDg
Length = 318
Score = 36.7 bits (81), Expect = 0.57
Identities = 27/95 (28%), Positives = 47/95 (49%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHH 563
N+A+VI++ G +I + K H DF+ + G V + K+ + ICY R +
Sbjct: 131 NSAMVIDKNGNIIMTYSKVH---TCDFSLESLVESGEE-FKVCDFHGIKLGVMICYDREY 186
Query: 564 PLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEAR 668
P + M + GAEI+ P+ + G+ + A+ R
Sbjct: 187 PESARMLMLKGAEIIVVPN-SCGGMKPRVQALSTR 220
>UniRef50_Q9PC24 Cluster: Probable glutamine-dependent NAD(+)
synthetase (EC 6.3.5.1) (NAD(+) synthase
[glutamine-hydrolysing]); n=9; Proteobacteria|Rep:
Probable glutamine-dependent NAD(+) synthetase (EC
6.3.5.1) (NAD(+) synthase [glutamine-hydrolysing]) -
Xylella fastidiosa
Length = 545
Score = 36.7 bits (81), Expect = 0.57
Identities = 35/116 (30%), Positives = 53/116 (45%), Gaps = 8/116 (6%)
Frame = +3
Query: 369 GDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYY-FEGNTGHPVFETKYGKVAINI 545
G ++N A V+ + G+V +RK +P F+E Y+ + N VF+ K V + I
Sbjct: 92 GSVVYNVASVLCD-GQVEQTYRKRELPNYAVFDERRYFEVDPNGSRCVFKVKGVPVGVLI 150
Query: 546 CYGR--HHPLNWLMFGINGAEIVFNPSATVSGLSEH-----LWAVEARNAAIANSY 692
C PL + G GAE+V P+A+ +H L A AR A +Y
Sbjct: 151 CEDLWFSEPLADTVCG--GAELVLVPNASPYERGKHAQRDALLAERARETGAAIAY 204
>UniRef50_Q5NN79 Cluster: Nitrilase; n=17; Proteobacteria|Rep:
Nitrilase - Zymomonas mobilis
Length = 329
Score = 36.3 bits (80), Expect = 0.75
Identities = 32/133 (24%), Positives = 57/133 (42%), Gaps = 1/133 (0%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
G T + A K ++ ++ER + T++ TA+ G +IGKHRK +P
Sbjct: 83 GKETARIGSFAAKMKAYLVVGVIERSEA---TLYCTALFFAPDGTLIGKHRK-LMPTA-- 136
Query: 462 FNESTYYFEGN-TGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGL 638
E + +G+ + + +T GK+ IC+ + P+ + G I P+
Sbjct: 137 -TERLVWGQGDGSTIEILDTAVGKLGAAICWENYMPVLRQVMYAGGVNIWCAPTVD---- 191
Query: 639 SEHLWAVEARNAA 677
+W V R+ A
Sbjct: 192 QREIWQVSMRHIA 204
>UniRef50_Q2SQI0 Cluster: Predicted amidohydrolase; n=1; Hahella
chejuensis KCTC 2396|Rep: Predicted amidohydrolase -
Hahella chejuensis (strain KCTC 2396)
Length = 265
Score = 36.3 bits (80), Expect = 0.75
Identities = 44/163 (26%), Positives = 71/163 (43%), Gaps = 1/163 (0%)
Frame = +3
Query: 147 VQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYD 326
V ++++ A +V+++C EA+ +P K C P + + +LA K
Sbjct: 21 VDRLVTEATLSKVDLICFPEAY-VPGIRGMDFKVENCS---PRKMQSALDRICDLARKTS 76
Query: 327 MVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP 506
+ II P +E G N A V++ G ++GK KNH+ + E Y+ G
Sbjct: 77 INIILP-MEWPAPEGRR--NVAFVVDRRGVLLGKQTKNHLEQ----GEEAYFIPG-ARRQ 128
Query: 507 VFETKYGKVAINICY-GRHHPLNWLMFGINGAEIVFNPSATVS 632
+F+ K I I + G P GA+IVF+P T S
Sbjct: 129 LFDADGVKFGIVISHEGWRCPETVRWAASRGAKIVFHPHFTGS 171
>UniRef50_O94660 Cluster: Nitrilase; n=6; Ascomycota|Rep: Nitrilase
- Schizosaccharomyces pombe (Fission yeast)
Length = 276
Score = 36.3 bits (80), Expect = 0.75
Identities = 33/138 (23%), Positives = 53/138 (38%), Gaps = 5/138 (3%)
Frame = +3
Query: 306 ELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFN-----E 470
E A K+ + + + E + + ++ + G++I ++ K H+ V N E
Sbjct: 71 ESATKHSIFVNICVHEPSKVKNKLLNSSLFIEPLHGEIISRYSKAHLFDVEIKNGPTLKE 130
Query: 471 STYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHL 650
S G P +T GKV IC+ P + GA I+ PSA
Sbjct: 131 SNTTLRGEAILPPCKTPLGKVGSAICFDIRFPEQAIKLRNMGAHIITYPSAFTEKTGAAH 190
Query: 651 WAVEARNAAIANSYYTCA 704
W V R A+ + Y A
Sbjct: 191 WEVLLRARALDSQCYVIA 208
>UniRef50_A6UC57 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Sinorhizobium|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Sinorhizobium medicae
WSM419
Length = 258
Score = 35.9 bits (79), Expect = 0.99
Identities = 34/128 (26%), Positives = 58/128 (45%)
Frame = +3
Query: 258 DFAEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRK 437
+ AEP GP L ++ + II+ E+ G T++N+AV ++ +RK
Sbjct: 55 ELAEPP-DGPIVQELRRISRQTGTAIIAGFAEQS---GHTVYNSAVHVDG-DTAPTVYRK 109
Query: 438 NHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNP 617
+H+ GD+ E + + +FE + + ICY P N + GA+ V P
Sbjct: 110 SHL--YGDY-ERSLFSPAEPSTRLFEHRGVTCGMLICYDVEFPENVRRLALAGADAVLVP 166
Query: 618 SATVSGLS 641
+A +G S
Sbjct: 167 TALPAGWS 174
>UniRef50_A6GKJ0 Cluster: Putative uncharacterized protein; n=1;
Plesiocystis pacifica SIR-1|Rep: Putative
uncharacterized protein - Plesiocystis pacifica SIR-1
Length = 554
Score = 35.9 bits (79), Expect = 0.99
Identities = 34/118 (28%), Positives = 54/118 (45%), Gaps = 3/118 (2%)
Frame = +3
Query: 285 PSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDF 464
P AELA + D ++ LE D D +NT V ++ G V G H K + G+
Sbjct: 374 PLLARFAELADEVDAYVVIN-LETIDPASDARYNTVVALDPEGAVAGTHHKFEL-YGGER 431
Query: 465 NESTYYFEGNTGHPVFETKYGKVAINIC---YGRHHPLNWLMFGINGAEIVFNPSATV 629
+ T +T F+T +G+V + C YGR H L+ G++ + ++ TV
Sbjct: 432 DALTPGGAVST----FDTPFGRVGLLTCADIYGRPHLHEELVNGLDARIVAWSAEWTV 485
>UniRef50_Q5V604 Cluster: Nitrilase; n=2; Halobacteriaceae|Rep:
Nitrilase - Haloarcula marismortui (Halobacterium
marismortui)
Length = 366
Score = 35.9 bits (79), Expect = 0.99
Identities = 26/104 (25%), Positives = 46/104 (44%), Gaps = 2/104 (1%)
Frame = +3
Query: 300 LAELAVKYDMVIISPILERDDIHG-DTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNEST 476
L E + D+ ++ E D G +T++N+ + G+++G+HRK + E
Sbjct: 86 LGEAVAEADLTLVLGTNEISDRQGSETLYNSLFYFDNTGELMGRHRK----LMPTHEERA 141
Query: 477 YYFEGNTGH-PVFETKYGKVAINICYGRHHPLNWLMFGINGAEI 605
+ G+ +ET G++ ICY H L+ G EI
Sbjct: 142 IWGRGDPSSLATYETDVGRLGGLICYENHMTLSKAALTTMGEEI 185
>UniRef50_O76464 Cluster: Nitrilase and fragile histidine triad
fusion protein NitFhit (NFT-1 protein) [Includes:
Bis(5'-adenosyl)-triphosphatase (EC 3.6.1.29)
(Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
(Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
Nitrilase homolog (EC 3.5.-.-)]; n=18; Eumetazoa|Rep:
Nitrilase and fragile histidine triad fusion protein
NitFhit (NFT-1 protein) [Includes:
Bis(5'-adenosyl)-triphosphatase (EC 3.6.1.29)
(Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
(Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
Nitrilase homolog (EC 3.5.-.-)] - Drosophila
melanogaster (Fruit fly)
Length = 460
Score = 35.9 bits (79), Expect = 0.99
Identities = 48/202 (23%), Positives = 81/202 (40%), Gaps = 6/202 (2%)
Frame = +3
Query: 117 TQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTV 296
T + A +V +++ A ++ +L L E + F + + +E L G
Sbjct: 43 TSDKAANLSQVIELVDRAKSQNACMLFLPECCD----FVGESRTQTIELSEG-LDGELMA 97
Query: 297 FLAELAVKYDMVIIS--PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD--- 461
ELA K + + IS + ER+D I+N V++NE G++ +RK H+ V
Sbjct: 98 QYRELA-KCNKIWISLGGVHERND---QKIFNAHVLLNEKGELAAVYRKLHMFDVTTKEV 153
Query: 462 -FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGL 638
ES G T G++ + ICY ++ GA ++ PSA
Sbjct: 154 RLRESDTVTPGYCLERPVSTPVGQIGLQICYDLRFAEPAVLLRKLGANLLTYPSAFTYAT 213
Query: 639 SEHLWAVEARNAAIANSYYTCA 704
+ W + R AI + A
Sbjct: 214 GKAHWEILLRARAIETQCFVVA 235
>UniRef50_Q1GRP3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Sphingomonadales|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Sphingopyxis
alaskensis (Sphingomonas alaskensis)
Length = 286
Score = 35.5 bits (78), Expect = 1.3
Identities = 28/112 (25%), Positives = 49/112 (43%), Gaps = 5/112 (4%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINIC 548
N + VI G++ ++ K H +P ++ ES Y G + +T G++ ++IC
Sbjct: 103 NRSHVIAADGRIRARYDKIHMFDVQLPSGENWQESAAY-AGGDALCIVDTPLGRLGLSIC 161
Query: 549 YGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
Y P + +GA ++ P+A E W V R AI + + A
Sbjct: 162 YDLRFPELYRALVDSGATLIAIPAAFTVPTGEAHWHVLLRARAIETACHVVA 213
>UniRef50_Q0EPQ3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase precursor; n=1;
Thermoanaerobacter ethanolicus X514|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase precursor - Thermoanaerobacter
ethanolicus X514
Length = 360
Score = 35.5 bits (78), Expect = 1.3
Identities = 23/84 (27%), Positives = 39/84 (46%)
Frame = +3
Query: 378 IWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGR 557
++N +++ GK++G+ +K H+ DF E N +F GKVA +C
Sbjct: 165 LYNGGALVSREGKILGRQKKIHLT---DFEEKIGLKRENELE-IFSLDIGKVACPVCMDA 220
Query: 558 HHPLNWLMFGINGAEIVFNPSATV 629
+ + + GAEIV P A +
Sbjct: 221 TYFETFKIASQKGAEIVILPIANM 244
>UniRef50_A7DA57 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2; Methylobacterium
extorquens PA1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Methylobacterium
extorquens PA1
Length = 369
Score = 35.5 bits (78), Expect = 1.3
Identities = 26/99 (26%), Positives = 43/99 (43%), Gaps = 1/99 (1%)
Frame = +3
Query: 276 LTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRV 455
L GP + A ++ +++ E + +WN V+I G ++ HRK V
Sbjct: 80 LDGPEIGAVRAAARRHGVLVSLGFSESTEASVGCLWNANVLIGRDGAILNHHRK----LV 135
Query: 456 GDFNESTYYFEGNT-GHPVFETKYGKVAINICYGRHHPL 569
F E + G+ G V T+ G+V + IC +PL
Sbjct: 136 PTFYEKLIWANGDARGLRVTRTEIGRVGMLICGENTNPL 174
>UniRef50_A3SP65 Cluster: Possible nitrilase; n=2;
Rhodobacteraceae|Rep: Possible nitrilase - Roseovarius
nubinhibens ISM
Length = 284
Score = 35.5 bits (78), Expect = 1.3
Identities = 28/109 (25%), Positives = 44/109 (40%), Gaps = 5/109 (4%)
Frame = +3
Query: 384 NTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP-----VFETKYGKVAINIC 548
N +I G ++G++ K H+ V D Y E T P + +T ++ IC
Sbjct: 100 NRGYMIAPDGSIVGRYDKIHLFDV-DLGPGQSYRESATVAPGGQAVIHDTPKARIGHAIC 158
Query: 549 YGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYY 695
Y P + GAEI+ P+A E W + R AI + +
Sbjct: 159 YDLRFPALFHTLACEGAEILCCPAAFTKLTGEAHWHILNRARAIETTRF 207
>UniRef50_A1ZR32 Cluster: Hydrolase, carbon-nitrogen family; n=2;
Bacteria|Rep: Hydrolase, carbon-nitrogen family -
Microscilla marina ATCC 23134
Length = 289
Score = 35.5 bits (78), Expect = 1.3
Identities = 29/111 (26%), Positives = 53/111 (47%), Gaps = 2/111 (1%)
Frame = +3
Query: 300 LAELAVKYDMVII-SPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNEST 476
+ ++A KY + ++ + E+ + + I+NTA VIN G+V+ ++ K + F
Sbjct: 45 MQKMAKKYGIWLVPGSVFEKRE---NLIYNTASVINPQGEVVTRYSK-----MFPFYPYE 96
Query: 477 YYFEGNTGHPVFET-KYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSAT 626
+ VF+ GK I+ICY P + GAE++ +P+ T
Sbjct: 97 VGVTPGSQFCVFDVPNVGKFGISICYDMWFPETIRTLTVMGAEVILHPTMT 147
>UniRef50_A0Y2B3 Cluster: Putative hydrolase, carbon-nitrogen family
protein; n=3; Alteromonadales|Rep: Putative hydrolase,
carbon-nitrogen family protein - Alteromonadales
bacterium TW-7
Length = 279
Score = 35.5 bits (78), Expect = 1.3
Identities = 25/107 (23%), Positives = 42/107 (39%), Gaps = 6/107 (5%)
Frame = +3
Query: 402 NEFGKVIGKHRKNHI------PRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHH 563
N G+ + + K H+ + G + ES + G+ V E+ +GK+ + +CY
Sbjct: 108 NNQGECVATYNKIHLFDVNVDDKTGSYRESDFTQAGSDV-VVVESPFGKLGLTVCYDLRF 166
Query: 564 PLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
+ GAE++ PSA + W AI Y A
Sbjct: 167 SALFTALARKGAEVILVPSAFTMVTGQAHWQPLLAARAIETQCYVVA 213
>UniRef50_A0M3E2 Cluster: Carbon-nitrogen hydrolase; n=6; cellular
organisms|Rep: Carbon-nitrogen hydrolase - Gramella
forsetii (strain KT0803)
Length = 311
Score = 35.5 bits (78), Expect = 1.3
Identities = 29/107 (27%), Positives = 48/107 (44%), Gaps = 1/107 (0%)
Frame = +3
Query: 378 IWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETK-YGKVAINICYG 554
I+NTA VIN G+V+ ++RK + F + VF+ K I+ICY
Sbjct: 90 IYNTASVINPEGEVVTRYRK-----MFPFYPYEVGVTPGSQFCVFDVPGVAKFGISICYD 144
Query: 555 RHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYY 695
P + GAE++ +P+ T + E ++ AA+ Y+
Sbjct: 145 MWFPETVRTLSVMGAEVILHPTMTGTIDREIELSIVRAMAAVNQCYF 191
>UniRef50_A0CJZ7 Cluster: Chromosome undetermined scaffold_2, whole
genome shotgun sequence; n=2; Oligohymenophorea|Rep:
Chromosome undetermined scaffold_2, whole genome shotgun
sequence - Paramecium tetraurelia
Length = 274
Score = 35.5 bits (78), Expect = 1.3
Identities = 25/93 (26%), Positives = 46/93 (49%)
Frame = +3
Query: 168 AAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPI 347
++ ++++IL L E + + + +K F E GP+ F ++A + +
Sbjct: 35 SSKDEIDILVLPEMALIGYYY--PDKNAIKPFLEQYGKGPTYEFCKQIAQRLKCYVSCGY 92
Query: 348 LERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI 446
E D GD ++N+AVV+N G+ I RK H+
Sbjct: 93 AEVD---GDKLYNSAVVVNREGEAILNVRKKHL 122
>UniRef50_Q5AHS1 Cluster: Potential N-terminal amidase; n=2; Candida
albicans|Rep: Potential N-terminal amidase - Candida
albicans (Yeast)
Length = 374
Score = 35.5 bits (78), Expect = 1.3
Identities = 19/57 (33%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIIS--PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHI 446
GPS EL++KY +++ P DD + I+N+ V N FG++I +RK+ +
Sbjct: 89 GPSLNLGRELSIKYQSILVIGYPEFSHDD---NKIYNSCAVFNRFGQLIYNYRKSFL 142
>UniRef50_Q8TLM7 Cluster: Carbon-nitrogen hydrolase; n=2;
Methanosarcina|Rep: Carbon-nitrogen hydrolase -
Methanosarcina acetivorans
Length = 309
Score = 35.5 bits (78), Expect = 1.3
Identities = 28/104 (26%), Positives = 47/104 (45%), Gaps = 1/104 (0%)
Frame = +3
Query: 405 EFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYG-KVAINICYGRHHPLNWLM 581
E G + G + K H + E+ Y+ +G++ P+ K K+ + ICY P
Sbjct: 148 ESGVLAGTYLKTHPFKA----ENGYFSKGSSIEPISLKKQNLKIGLEICYELRFPEVARK 203
Query: 582 FGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
I G++++ +A + +EH W A+ AI N A NR
Sbjct: 204 LSIAGSDLLVTIAAFPNPRAEH-WKTLAKARAIENQIPHIACNR 246
>UniRef50_UPI000023E394 Cluster: hypothetical protein FG01991.1;
n=1; Gibberella zeae PH-1|Rep: hypothetical protein
FG01991.1 - Gibberella zeae PH-1
Length = 319
Score = 35.1 bits (77), Expect = 1.7
Identities = 32/108 (29%), Positives = 49/108 (45%), Gaps = 11/108 (10%)
Frame = +3
Query: 375 TIWNTAVVINEFGKVIGKHRKNH--IPR---VGDF-----NESTYYFEGNTGHPVFETKY 524
T+ NT+ I+ G ++G + K + IP + F N S F H V +T
Sbjct: 111 TLLNTSDFIDHDGNLLGTYTKTNLWIPERLTLTSFVDHARNTSKDEFAAPNPHQVIDTPL 170
Query: 525 GKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSG-LSEHLWAVEA 665
G+V I +C+ P + + GA+I+ PS SG +SE A A
Sbjct: 171 GRVGILVCWDLAFPEAFRQLVLAGAKIIIIPSYWTSGDMSEEGLAYNA 218
>UniRef50_Q11M91 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Mesorhizobium sp.
BNC1|Rep: Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Mesorhizobium sp. (strain BNC1)
Length = 272
Score = 35.1 bits (77), Expect = 1.7
Identities = 28/113 (24%), Positives = 44/113 (38%), Gaps = 5/113 (4%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNH-----IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINI 545
+NT+VVI GK + + K H +P + ES GN ++ V +++
Sbjct: 95 YNTSVVIGPDGKQLATYDKIHRYDVDLPSGLSYRESDTNDAGNVA-VTYDHNGTNVGLSV 153
Query: 546 CYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCA 704
CY +L GA+++ P+A W R AI Y A
Sbjct: 154 CYDVRFGSLYLELAARGAQVITIPAAFTFETGAAHWDTLVRARAIETQCYVAA 206
>UniRef50_A1HU09 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Thermosinus
carboxydivorans Nor1|Rep: Nitrilase/cyanide hydratase
and apolipoprotein N-acyltransferase - Thermosinus
carboxydivorans Nor1
Length = 275
Score = 35.1 bits (77), Expect = 1.7
Identities = 23/84 (27%), Positives = 33/84 (39%), Gaps = 3/84 (3%)
Frame = +3
Query: 468 ESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSAT---VSGL 638
E +Y G+ PVF +V +C +H+P + GAE++ P AT
Sbjct: 123 EKKHYAAGDF-LPVFALPEARVGFQLCLEQHYPEITQTLALRGAELILCPHATPRLTPAE 181
Query: 639 SEHLWAVEARNAAIANSYYTCAIN 710
W + R A N Y A N
Sbjct: 182 RRDSWHISLRARAYDNCVYILATN 205
>UniRef50_A1HNR2 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=3; Firmicutes|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Thermosinus carboxydivorans Nor1
Length = 284
Score = 35.1 bits (77), Expect = 1.7
Identities = 26/88 (29%), Positives = 43/88 (48%), Gaps = 3/88 (3%)
Frame = +3
Query: 372 DTIWNTAVVINEFGKVIGKHRKNHIP-RVGDFNESTYYFEGNTGHP--VFETKYGKVAIN 542
+ ++N A + GK++ + + + P V ++N + GH VFET+ G +AI
Sbjct: 99 ERLYNVAHLFYPNGKIVRQPKLHITPTEVKEWNMAA-------GHDINVFETEKGTIAIL 151
Query: 543 ICYGRHHPLNWLMFGINGAEIVFNPSAT 626
CY P M GA+++F PS T
Sbjct: 152 TCYDIEFPEIVRMVRAKGADVIFCPSCT 179
>UniRef50_A0H2F4 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Chloroflexus
aggregans DSM 9485|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Chloroflexus
aggregans DSM 9485
Length = 322
Score = 35.1 bits (77), Expect = 1.7
Identities = 22/83 (26%), Positives = 36/83 (43%)
Frame = +3
Query: 372 DTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICY 551
D +++ AV+ G G+ K ++ D + G V T +G V + +CY
Sbjct: 134 DGLYDAAVLFGPDGSERGRQAKINVT---DEERQAGFIAGPRRSVVVTTPFGIVGLGVCY 190
Query: 552 GRHHPLNWLMFGINGAEIVFNPS 620
RH P M G++IV P+
Sbjct: 191 DRHTPEVTRMLAKAGSQIVAMPA 213
>UniRef50_Q9X0Y0 Cluster: Probable glutamine-dependent NAD(+)
synthetase (EC 6.3.5.1) (NAD(+) synthase
[glutamine-hydrolyzing]); n=6; Bacteria|Rep: Probable
glutamine-dependent NAD(+) synthetase (EC 6.3.5.1)
(NAD(+) synthase [glutamine-hydrolyzing]) - Thermotoga
maritima
Length = 576
Score = 35.1 bits (77), Expect = 1.7
Identities = 26/87 (29%), Positives = 43/87 (49%), Gaps = 5/87 (5%)
Frame = +3
Query: 381 WNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYG--KVAINICYG 554
+N A V+ + G+++G +RK +P G F+E Y+ G + K G KV + IC
Sbjct: 95 YNAAAVVKD-GEILGVYRKISLPNYGVFDERRYF---KPGEELLVVKIGNIKVGVTICED 150
Query: 555 RHHPLN---WLMFGINGAEIVFNPSAT 626
+P+ L G G ++ N SA+
Sbjct: 151 IWNPVEPSASLSLG-EGVHLIANLSAS 176
>UniRef50_Q6RWG5 Cluster: Nitrilase; n=2; uncultured organism|Rep:
Nitrilase - uncultured organism
Length = 309
Score = 34.7 bits (76), Expect = 2.3
Identities = 18/52 (34%), Positives = 31/52 (59%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRK 437
GP+T +A+LA + + I+ ER G+T++NTA+ G+++ HRK
Sbjct: 86 GPATARIAKLAGELGVTIVVGAHERA---GNTLYNTALTFGPEGRLLNHHRK 134
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 697,220,589
Number of Sequences: 1657284
Number of extensions: 14243797
Number of successful extensions: 33302
Number of sequences better than 10.0: 279
Number of HSP's better than 10.0 without gapping: 32123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33167
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 57438021881
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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