Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0016
(715 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 66 5e-12
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 60 2e-10
SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual 36 0.004
SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 28 1.5
SPAC30C2.03 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 1.5
SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe... 27 2.0
SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D |Schi... 27 2.0
SPAC3H1.10 |||phytochelatin synthetase |Schizosaccharomyces pomb... 27 2.7
SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr ... 26 4.7
SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyce... 25 8.1
SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces pom... 25 8.1
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 66.1 bits (154), Expect = 5e-12
Identities = 42/150 (28%), Positives = 74/150 (49%)
Frame = +3
Query: 264 AEPVLTGPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH 443
AE GPS ++ LA KY + II E+++ + I+N+ + I E G + G +RK H
Sbjct: 61 AEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEKQSNIIYNSCIYITENGNLGGVYRKVH 120
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+ F+ +F+ + P+FET +GK+ + IC+ P + +NGA+++ +
Sbjct: 121 L-----FDTERKHFKKGSDFPIFETSFGKLGVMICWDTAFPEVARIHALNGADLLVVATN 175
Query: 624 TVSGLSEHLWAVEARNAAIANSYYTCAINR 713
+ S+ W + + A N A NR
Sbjct: 176 WENPYSDD-WDLVTKARAFENCIPLVAANR 204
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 60.5 bits (140), Expect = 2e-10
Identities = 61/220 (27%), Positives = 93/220 (42%), Gaps = 7/220 (3%)
Frame = +3
Query: 57 RIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCT 236
R R+GL+Q + + RL + E AA N++ L E +N P+
Sbjct: 42 RAFRIGLVQLANTKDKSENLQLARLKVLE--------AAKNGSNVIVLPEIFNSPYG--- 90
Query: 237 REKQPWCDFAEPVL-TGPSTVFLAELAVKYDMVIIS-PILERDDIHGDTIWNTAVVINEF 410
+ +AEP+ + PS L+ +A + I ER D ++NTA+V +
Sbjct: 91 --TGYFNQYAEPIEESSPSYQALSSMAKDTKTYLFGGSIPERKD---GKLYNTAMVFDPS 145
Query: 411 GKVIGKHRKNH-----IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNW 575
GK+I HRK H IP F ES G+ V +T+YGK + ICY P
Sbjct: 146 GKLIAVHRKIHLFDIDIPGGVSFRESDSLSPGDAMTMV-DTEYGKFGLGICYDIRFPELA 204
Query: 576 LMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYY 695
++ NG ++ P A W + AR A+ N +
Sbjct: 205 MIAARNGCSVMIYPGAFNLSTGPLHWELLARARAVDNEMF 244
>SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 276
Score = 36.3 bits (80), Expect = 0.004
Identities = 33/138 (23%), Positives = 53/138 (38%), Gaps = 5/138 (3%)
Frame = +3
Query: 306 ELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFN-----E 470
E A K+ + + + E + + ++ + G++I ++ K H+ V N E
Sbjct: 71 ESATKHSIFVNICVHEPSKVKNKLLNSSLFIEPLHGEIISRYSKAHLFDVEIKNGPTLKE 130
Query: 471 STYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHL 650
S G P +T GKV IC+ P + GA I+ PSA
Sbjct: 131 SNTTLRGEAILPPCKTPLGKVGSAICFDIRFPEQAIKLRNMGAHIITYPSAFTEKTGAAH 190
Query: 651 WAVEARNAAIANSYYTCA 704
W V R A+ + Y A
Sbjct: 191 WEVLLRARALDSQCYVIA 208
>SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1234
Score = 27.9 bits (59), Expect = 1.5
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = +3
Query: 600 EIVFNPSATVSGLSEHLWAVEARNAAIA 683
EI+ +PS +S +S+H W + R+ I+
Sbjct: 374 EIMKHPSLLISAISQHFWVLALRDPIIS 401
>SPAC30C2.03 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 210
Score = 27.9 bits (59), Expect = 1.5
Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 2/49 (4%)
Frame = +3
Query: 84 HSIAIS--TDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPF 224
HSI++ + I+Q+ + I QK+ A + + + C+ AW +PF
Sbjct: 26 HSISLGYLSKEKISQEEMQIIRATQKL---ARTKYMTLYCIPRAWLIPF 71
>SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 27.5 bits (58), Expect = 2.0
Identities = 17/59 (28%), Positives = 26/59 (44%)
Frame = +3
Query: 534 AINICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAIN 710
A+ CY +H W+ + + E+ NP T + L LW V+A I+ A N
Sbjct: 332 ALKACYRKH----WVQYILEQNELGRNPYNTTNVLRAILWLVKAWTTDISPEIIENAFN 386
>SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1107
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/29 (44%), Positives = 19/29 (65%), Gaps = 2/29 (6%)
Frame = -1
Query: 253 HGCFSLVQNAKGMFQA--SCKQRILTCSA 173
+GCF+ QN KGM +A ++R LTC +
Sbjct: 976 YGCFTDFQNVKGMSKAIFIVQRRALTCKS 1004
>SPAC3H1.10 |||phytochelatin synthetase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 414
Score = 27.1 bits (57), Expect = 2.7
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +3
Query: 354 RDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNES 473
+D I TI N + I+ KV+G+ H VG F+ES
Sbjct: 168 KDVISCSTIENKIMAISFCRKVLGQTGDGHFSPVGGFSES 207
>SPCC320.09 |hem15||ferrochelatase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 384
Score = 26.2 bits (55), Expect = 4.7
Identities = 19/63 (30%), Positives = 27/63 (42%), Gaps = 4/63 (6%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHR----KNHIP 449
GP+ V + + ++ + P LER GD I N GK I K R +NH
Sbjct: 31 GPTAVVMMNMGGPSNLDEVGPFLERLFTDGDII-PLGYFQNSLGKFIAKRRTPKVQNHYS 89
Query: 450 RVG 458
+G
Sbjct: 90 DIG 92
>SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 610
Score = 25.4 bits (53), Expect = 8.1
Identities = 10/30 (33%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = +3
Query: 252 WCDF-AEPVLTGPSTVFLAELAVKYDMVII 338
+C+F + P+L P V + +LA YD ++
Sbjct: 372 FCEFPSNPLLNSPDLVRIRKLADSYDFAVV 401
>SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 592
Score = 25.4 bits (53), Expect = 8.1
Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 3/36 (8%)
Frame = +3
Query: 399 INEFGKVIGKHRKNHIPRVGDF---NESTYYFEGNT 497
+ + GK +GKHRKN + R G F EST G T
Sbjct: 90 LKDEGK-LGKHRKNRLRRPGRFPVRQESTIDIPGLT 124
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,027,982
Number of Sequences: 5004
Number of extensions: 66101
Number of successful extensions: 166
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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