Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0016
(715 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 357 3e-99
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 82 3e-16
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 81 9e-16
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 64 7e-11
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 62 5e-10
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 52 5e-07
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 50 2e-06
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 48 5e-06
At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 45 6e-05
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 43 2e-04
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329... 34 0.081
At1g11660.1 68414.m01339 heat shock protein, putative strong sim... 32 0.33
At2g35060.1 68415.m04301 potassium transporter family protein si... 30 1.3
At4g04850.1 68417.m00707 K+ efflux antiporter, putative (KEA3) s... 30 1.8
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 357 bits (879), Expect = 3e-99
Identities = 163/237 (68%), Positives = 193/237 (81%)
Frame = +3
Query: 3 FEIKAYDFPARKEECRKPRIVRLGLIQHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQ 182
F+++A F A KE+ R PR+VR+GLIQ+SIA+ T P + Q IF+K++ II AA
Sbjct: 70 FDLQAASFSADKEQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAG 129
Query: 183 VNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVIISPILERDD 362
VNILCLQEAW MPFAFCTRE++ WC+FAEPV G ST FL ELA KY+MVI+SPILERD
Sbjct: 130 VNILCLQEAWTMPFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDI 187
Query: 363 IHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVFETKYGKVAIN 542
HG+ +WNTAV+I G +IGKHRKNHIPRVGDFNESTYY EG+TGHPVFET +GK+A+N
Sbjct: 188 DHGEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGKIAVN 247
Query: 543 ICYGRHHPLNWLMFGINGAEIVFNPSATVSGLSEHLWAVEARNAAIANSYYTCAINR 713
ICYGRHHPLNWL FG+NGAEIVFNPSATV LSE +W +EARNAAIANSY+ +INR
Sbjct: 248 ICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPMWPIEARNAAIANSYFVGSINR 304
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 82.2 bits (194), Expect = 3e-16
Identities = 47/159 (29%), Positives = 87/159 (54%), Gaps = 1/159 (0%)
Frame = +3
Query: 150 QKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDM 329
++++ A A+ NI+ +QE + + FC +++ + A+P P+ + +LA + +
Sbjct: 31 ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89
Query: 330 VI-ISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHP 506
VI +S E + H +N+ +I+ G +G +RK+HIP + E Y+ G+TG
Sbjct: 90 VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFK 145
Query: 507 VFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
VF+TK+ K+ + IC+ + P + GAEI+F P+A
Sbjct: 146 VFQTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTA 184
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 80.6 bits (190), Expect = 9e-16
Identities = 47/157 (29%), Positives = 85/157 (54%), Gaps = 1/157 (0%)
Frame = +3
Query: 156 IISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAVKYDMVI 335
++ A A+ NI+ +QE + + FC +++ + A+P P+ + +LA + +VI
Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118
Query: 336 -ISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGHPVF 512
+S E + H +N+ +I+ G +G +RK+HIP + E Y+ G+TG VF
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 174
Query: 513 ETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
+TK+ K+ + IC+ + P + GAEI+F P+A
Sbjct: 175 QTKFAKIGVAICWDQWFPEAARAMVLQGAEILFYPTA 211
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 64.5 bits (150), Expect = 7e-11
Identities = 58/209 (27%), Positives = 90/209 (43%), Gaps = 12/209 (5%)
Frame = +3
Query: 114 ITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTG--- 284
+T + +K I AA++ ++ L E WN P++ + +AE + G
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPYS-----NDSFPVYAEEIDAGGDA 151
Query: 285 -PSTVFLAELAVKYDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH----- 443
PST L+E++ + + II I ER GD ++NT V G++ KHRK H
Sbjct: 152 SPSTAMLSEVSKRLKITIIGGSIPERV---GDRLYNTCCVFGSDGELKAKHRKIHLFDID 208
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
IP F ES G T + +T G++ I ICY +++ GA ++ P A
Sbjct: 209 IPGKITFMESKTLTAGET-PTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGA 267
Query: 624 TVSGLSEHLWAVEARNAAIANSYY--TCA 704
W + R A N Y TC+
Sbjct: 268 FNMTTGPLHWELLQRARATDNQLYVATCS 296
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 61.7 bits (143), Expect = 5e-10
Identities = 55/204 (26%), Positives = 87/204 (42%), Gaps = 10/204 (4%)
Frame = +3
Query: 114 ITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTG--- 284
+T + +K I AA++ ++ L E WN P++ + +AE + G
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPYS-----NDSFPVYAEEIDAGGDA 151
Query: 285 -PSTVFLAELAVKYDMVIIS-PILERDDIHGDTIWNTAVVINEFGKVIGKHRKNH----- 443
PST L+E++ + + II I ER GD ++NT V G++ KHRK H
Sbjct: 152 SPSTAMLSEVSKRLKITIIGGSIPERV---GDRLYNTCCVFGSDGELKAKHRKIHLFDID 208
Query: 444 IPRVGDFNESTYYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
IP F ES G T + +T G++ I ICY +++ GA ++ P A
Sbjct: 209 IPGKITFMESKTLTAGET-PTIVDTDVGRIGIGICYDIRFQELAMIYAARGAHLLCYPGA 267
Query: 624 TVSGLSEHLWAVEARNAAIANSYY 695
W + R A N +
Sbjct: 268 FNMTTGPLHWELLQRARATDNQVH 291
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 51.6 bits (118), Expect = 5e-07
Identities = 40/145 (27%), Positives = 65/145 (44%), Gaps = 1/145 (0%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
GP LA +A KY + ++ ++ER+ G T++ T + + G +GKHRK +P
Sbjct: 115 GPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRKL-MPTA-- 168
Query: 462 FNESTYYFEGNTGHPVFETKYGKVAINICYGRHHP-LNWLMFGINGAEIVFNPSATVSGL 638
+ F + PVF+T GK+ IC+ P L M+ G EI P+A
Sbjct: 169 LERCIWGFGDGSTIPVFDTPIGKIGAAICWENRMPSLRTAMYA-KGIEIYCAPTAD---- 223
Query: 639 SEHLWAVEARNAAIANSYYTCAINR 713
S W + A+ + + N+
Sbjct: 224 SRETWLASMTHIALEGGCFVLSANQ 248
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 50.0 bits (114), Expect = 2e-06
Identities = 37/118 (31%), Positives = 58/118 (49%), Gaps = 1/118 (0%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
GP LAELA K ++ ++ +E+D G T++ TA+ + G+ +GKHRK +
Sbjct: 104 GPEVERLAELAGKNNVHLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRKVMPTSL-- 158
Query: 462 FNESTYYFEGNTGH-PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSATVS 632
E + +G+ PV++T GK+ IC+ PL G EI P+A S
Sbjct: 159 --ERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYRTALYAKGIEIYCAPTADYS 214
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 48.4 bits (110), Expect = 5e-06
Identities = 35/115 (30%), Positives = 57/115 (49%), Gaps = 1/115 (0%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
GP LAELA K ++ ++ +E+D G T++ TA+ + G+ +GKHRK +
Sbjct: 97 GPEVEKLAELAGKNNVYLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRKLMPTSL-- 151
Query: 462 FNESTYYFEGNTGH-PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
E + +G+ PV++T GK+ IC+ PL G E+ P+A
Sbjct: 152 --ERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTA 204
>At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1
[Mus musculus] GI:3228668; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 307
Score = 44.8 bits (101), Expect = 6e-05
Identities = 48/197 (24%), Positives = 81/197 (41%), Gaps = 8/197 (4%)
Frame = +3
Query: 138 FEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREKQPWCDFAEPVLTGPSTVFLAELAV 317
F +++ AA ++C E F+F ++ AEP L GP LA
Sbjct: 54 FATCSRLVQEAALAGAKLICFPEN----FSFVGDKEGESVKIAEP-LDGPVMERYCSLAR 108
Query: 318 KYDMVI-ISPILER-DDIHGDTIWNTAVVINEFGKVIGKHRKNH-----IPRVGDFNEST 476
++ + + ER DD H + NT VVI++ G + ++K H +P + ES+
Sbjct: 109 DSNIWLSLGGFQERFDDTH---LCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESS 165
Query: 477 YYFEGNTGHPVFETKYGKVAINICYGRHHPLNWLMFGING-AEIVFNPSATVSGLSEHLW 653
+ G V ++ G++ + +CY P + A+++ PSA E W
Sbjct: 166 FTVPGTKIVSV-DSPVGRLGLTVCYDLRFPKIYQQLRFEQKAQVLLVPSAFTKVTGEAHW 224
Query: 654 AVEARNAAIANSYYTCA 704
+ R AI Y A
Sbjct: 225 EILLRARAIETQCYVIA 241
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 42.7 bits (96), Expect = 2e-04
Identities = 31/115 (26%), Positives = 55/115 (47%), Gaps = 1/115 (0%)
Frame = +3
Query: 282 GPSTVFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIGKHRKNHIPRVGD 461
GP LA++A K + ++ +E++ G T++ T + + G+ +GKHRK +
Sbjct: 104 GPEVARLADVARKNHVYLVMGAIEKE---GYTLYCTVLFFSPQGQFLGKHRKLMPTSL-- 158
Query: 462 FNESTYYFEGNTGH-PVFETKYGKVAINICYGRHHPLNWLMFGINGAEIVFNPSA 623
E + +G+ PV++T GK+ IC+ PL G E+ P+A
Sbjct: 159 --ERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTA 211
>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 224
Score = 34.3 bits (75), Expect = 0.081
Identities = 24/86 (27%), Positives = 40/86 (46%), Gaps = 1/86 (1%)
Frame = +3
Query: 369 GDTIWNTAVVINEFGKVIGKHRKNHIPRVGDFNESTYYFEGNTGH-PVFETKYGKVAINI 545
G T++ T + + G+ +GKHRK + E + +G+ PV++T GK+ I
Sbjct: 8 GYTLYCTVLFFSPQGQFLGKHRKLMPTSL----ERCIWGQGDGSTIPVYDTPIGKLGAAI 63
Query: 546 CYGRHHPLNWLMFGINGAEIVFNPSA 623
C+ PL G E+ P+A
Sbjct: 64 CWENRMPLYRTALYAKGIELYCAPTA 89
>At1g11660.1 68414.m01339 heat shock protein, putative strong
similarity to gb|Z70314 heat-shock protein from
Arabidopsis thaliana and is a member of the PF|00012
Hsp70 protein family
Length = 773
Score = 32.3 bits (70), Expect = 0.33
Identities = 19/55 (34%), Positives = 33/55 (60%)
Frame = +3
Query: 81 QHSIAISTDNPITQQRLAIFEKVQKIISAAAAEQVNILCLQEAWNMPFAFCTREK 245
+++I + T+ + A EKV+K++SA A Q+NI CL E ++ +F RE+
Sbjct: 250 KYNIDVYTNTKACVRLRASCEKVKKVLSANAEAQLNIECLMEEKDVR-SFIKREE 303
>At2g35060.1 68415.m04301 potassium transporter family protein
similar to HAK2 [Hordeum vulgare] GI:7108599, potassium
transporter HAK2p [Mesembryanthemum crystallinum]
gi|14091471|gb|AAK53759; KUP/HAK/KT Transporter family
member, PMID:11500563; contains Pfam profile PF02705: K+
potassium transporter
Length = 792
Score = 30.3 bits (65), Expect = 1.3
Identities = 15/39 (38%), Positives = 20/39 (51%)
Frame = +1
Query: 142 KRFKRSYLLQLQSKLIFFVYKKLGTCPLHFVQEKSNHGV 258
K+F LLQL + + VY LGT PL+ HG+
Sbjct: 50 KKFSALLLLQLSFQSLGVVYGDLGTSPLYVFYNTFPHGI 88
>At4g04850.1 68417.m00707 K+ efflux antiporter, putative (KEA3)
similar to A. thaliana K+ antiporter KEA1, GenBank
accession number AF003382; Monovalent cation:proton
antiporter family 2 (CPA2 family) member, PMID:11500563
Length = 488
Score = 29.9 bits (64), Expect = 1.8
Identities = 15/44 (34%), Positives = 27/44 (61%)
Frame = +3
Query: 294 VFLAELAVKYDMVIISPILERDDIHGDTIWNTAVVINEFGKVIG 425
+ L ++AV +++I P+LE DI G++IW ++ E K +G
Sbjct: 121 LLLQDIAV-VPLLVILPVLESQDIGGESIW--PMLAKESAKALG 161
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,432,509
Number of Sequences: 28952
Number of extensions: 325329
Number of successful extensions: 666
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 639
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 651
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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