Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0004
(374 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g12200.1 68418.m01431 dihydropyrimidinase / DHPase / dihydrop... 100 4e-22
At3g55850.2 68416.m06206 amidohydrolase family protein weak simi... 33 0.082
At3g55850.1 68416.m06205 amidohydrolase family protein weak simi... 33 0.082
At4g04955.1 68417.m00720 amidohydrolase family protein similar t... 31 0.33
At4g06634.1 68417.m01050 zinc finger (C2H2 type) family protein ... 28 1.8
At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplas... 27 3.1
At2g17370.1 68415.m02006 3-hydroxy-3-methylglutaryl-CoA reductas... 27 4.1
At2g16870.1 68415.m01941 disease resistance protein (TIR-NBS-LRR... 27 4.1
At1g60060.1 68414.m06766 expressed protein 27 4.1
At1g40129.1 68414.m04766 hypothetical protein 26 7.1
At5g62420.1 68418.m07833 aldo/keto reductase family protein simi... 26 9.4
At3g24260.1 68416.m03047 hypothetical protein 26 9.4
At1g04670.1 68414.m00464 expressed protein ; expression supporte... 26 9.4
>At5g12200.1 68418.m01431 dihydropyrimidinase / DHPase /
dihydropyrimidine amidohydrolase / hydantoinase (PYD2)
identical to dihydropyrimidine amidohydrolase (PYD2)
[Arabidopsis thaliana] GI:28194047; similar to SP|Q9EQF5
Dihydropyrimidinase (EC 3.5.2.2) (DHPase) (Hydantoinase)
(DHP) {Mus musculus}; contains Pfam profile PF01979:
Amidohydrolase family
Length = 531
Score = 100 bits (239), Expect = 4e-22
Identities = 50/121 (41%), Positives = 68/121 (56%)
Frame = +1
Query: 10 GRIVNDDGIEEADVYIEDGIIKQVGRNLIIPGGTRTIDAAGKLVMPGGIDPHTHFELEMM 189
G +VN E ADVY+E+GII V N+ + +DA GK VMPGGIDPHTH +E M
Sbjct: 52 GTVVNAHHQELADVYVENGIIVAVQPNIKVGDEVTVLDATGKFVMPGGIDPHTHLAMEFM 111
Query: 190 GAKTADDFYKXXXXXXXXXXXXIIDFVLPEKGQSLLEAYANWREKADNKVCCDYGLHVGV 369
G +T DDF+ IDFV+P G +L+ + + K+ + C DYG H+ +
Sbjct: 112 GTETIDDFFSGQAAALAGGTTMHIDFVIPVNG-NLVAGFEAYENKS-RESCMDYGFHMAI 169
Query: 370 T 372
T
Sbjct: 170 T 170
>At3g55850.2 68416.m06206 amidohydrolase family protein weak
similarity to SP|Q06555 Exoenzymes regulatory protein
aepA precursor {Erwinia carotovora}; contains Pfam
profile PF01979: Amidohydrolase family
Length = 583
Score = 32.7 bits (71), Expect = 0.082
Identities = 20/55 (36%), Positives = 30/55 (54%), Gaps = 5/55 (9%)
Frame = +1
Query: 22 NDDGIEEAD-VYIEDGIIKQVGRNLIIPG----GTRTIDAAGKLVMPGGIDPHTH 171
+D + AD + I +G I +VG + G GT ++ GK+V+PG ID H H
Sbjct: 61 SDSSLPFADSMAIRNGRILKVGSFATLKGFIGDGTMEVNLEGKIVVPGLIDSHVH 115
>At3g55850.1 68416.m06205 amidohydrolase family protein weak
similarity to SP|Q06555 Exoenzymes regulatory protein
aepA precursor {Erwinia carotovora}; contains Pfam
profile PF01979: Amidohydrolase family
Length = 576
Score = 32.7 bits (71), Expect = 0.082
Identities = 20/55 (36%), Positives = 30/55 (54%), Gaps = 5/55 (9%)
Frame = +1
Query: 22 NDDGIEEAD-VYIEDGIIKQVGRNLIIPG----GTRTIDAAGKLVMPGGIDPHTH 171
+D + AD + I +G I +VG + G GT ++ GK+V+PG ID H H
Sbjct: 54 SDSSLPFADSMAIRNGRILKVGSFATLKGFIGDGTMEVNLEGKIVVPGLIDSHVH 108
>At4g04955.1 68417.m00720 amidohydrolase family protein similar to
SP|P32375 Allantoinase (EC 3.5.2.5) {Saccharomyces
cerevisiae}; contains Pfam profile PF01979:
Amidohydrolase family
Length = 506
Score = 30.7 bits (66), Expect = 0.33
Identities = 19/59 (32%), Positives = 29/59 (49%), Gaps = 4/59 (6%)
Frame = +1
Query: 13 RIVNDDGIEEADVYIEDGII----KQVGRNLIIPGGTRTIDAAGKLVMPGGIDPHTHFE 177
RIV +G+ V ++ GII K+V + + ID ++MPG ID H H +
Sbjct: 51 RIVTPNGLISGSVEVKGGIIVSVVKEVDWHKSQRSRVKVIDYGEAVLMPGLIDVHVHLD 109
>At4g06634.1 68417.m01050 zinc finger (C2H2 type) family protein
contains Pfam PF00096: Zinc finger, C2H2 type
Length = 387
Score = 28.3 bits (60), Expect = 1.8
Identities = 20/69 (28%), Positives = 31/69 (44%)
Frame = -3
Query: 252 CCPSGDRSPGALVEVIGSFSAHHFQLEVSVGVDAPGHDQFASGIDGPCASRNDEVAANLL 73
C PS DR E H ++L++ + + PGH Q D P ++++ N +
Sbjct: 222 CGPSSDRPYACPYEGCEKAYIHEYKLKLHLKREHPGHLQ-EENADTPTLNKHNGNDRNEI 280
Query: 72 DDAVFDVDV 46
DD D DV
Sbjct: 281 DDG-SDQDV 288
>At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplast,
mitochondrial / asparagine-tRNA ligase / AsnRS (SYNO)
nearly identical to SP|O48593
Length = 567
Score = 27.5 bits (58), Expect = 3.1
Identities = 15/30 (50%), Positives = 17/30 (56%)
Frame = -2
Query: 370 SLLRGDRSRSTPYYRLFPANSRRPLEATAL 281
SL R R R P + FPANSRR TA+
Sbjct: 33 SLFRPLRRRVLPPFDAFPANSRRRCFCTAV 62
>At2g17370.1 68415.m02006 3-hydroxy-3-methylglutaryl-CoA reductase 2
/ HMG-CoA reductase 2 (HMGR2) identical to SP|P43256
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 (EC
1.1.1.34) (HMG- CoA reductase 2) (HMGR2) {Arabidopsis
thaliana}
Length = 562
Score = 27.1 bits (57), Expect = 4.1
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = +2
Query: 215 TRAPGLRSPEGQQP*LTSFYLKKGSRF*RPTRIGGK 322
TRAP +R P ++ L FYL+ S F R + I K
Sbjct: 270 TRAPVVRFPSARRAALVMFYLQDPSNFERLSLIFNK 305
>At2g16870.1 68415.m01941 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1109
Score = 27.1 bits (57), Expect = 4.1
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = +3
Query: 87 QPHHSWRHTDHRC 125
Q HSW HTD RC
Sbjct: 900 QQQHSWEHTDIRC 912
>At1g60060.1 68414.m06766 expressed protein
Length = 386
Score = 27.1 bits (57), Expect = 4.1
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +3
Query: 87 QPHHSWRHTDHRCRWQTGHARGHRPPHSL 173
QP H + H+DH R+ GH H P ++
Sbjct: 249 QPEHQFYHSDHNHRFLIGH---HNQPQAV 274
>At1g40129.1 68414.m04766 hypothetical protein
Length = 567
Score = 26.2 bits (55), Expect = 7.1
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +1
Query: 76 QVGRNLIIPGGTRTIDAAGKLVMPGGIDPHTHFELE 183
QVG + +I T+ ++ +LV ++P HFE E
Sbjct: 518 QVGEDDVIMESTKDLEFDQELVAAKELEPEEHFEAE 553
>At5g62420.1 68418.m07833 aldo/keto reductase family protein similar
to chalcone reductase [Sesbania rostrata][GI:2792155];
contains Pfam profile PF00248: oxidoreductase, aldo/keto
reductase family
Length = 316
Score = 25.8 bits (54), Expect = 9.4
Identities = 10/36 (27%), Positives = 19/36 (52%)
Frame = +1
Query: 256 IIDFVLPEKGQSLLEAYANWREKADNKVCCDYGLHV 363
++DF + +E + WR++ KVC + +HV
Sbjct: 169 LLDFASVSPSVNQVEMHPLWRQRKLRKVCEENNIHV 204
>At3g24260.1 68416.m03047 hypothetical protein
Length = 374
Score = 25.8 bits (54), Expect = 9.4
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -2
Query: 361 RGDRSRSTPYYRLFPANSRRPLEATAL 281
R R+TP Y+L P + P+ +T L
Sbjct: 208 RRKTERATPNYKLIPKEEQTPVSSTVL 234
>At1g04670.1 68414.m00464 expressed protein ; expression supported
by MPSS
Length = 126
Score = 25.8 bits (54), Expect = 9.4
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -2
Query: 358 GDRSRSTPYYRLFPANSRRPLE 293
G +RS P YR F + S RPL+
Sbjct: 14 GKTARSVPLYRTFVSASPRPLQ 35
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,568,423
Number of Sequences: 28952
Number of extensions: 179898
Number of successful extensions: 465
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 452
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 464
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 507810264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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