Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brS-0003
(317 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65150.2 68414.m07387 meprin and TRAF homology domain-contain... 31 0.22
At1g65150.1 68414.m07386 meprin and TRAF homology domain-contain... 31 0.22
At2g47980.1 68415.m06004 expressed protein 28 1.2
At2g29780.1 68415.m03618 kelch repeat-containing F-box family pr... 28 1.6
At3g20660.1 68416.m02615 organic cation transporter family prote... 27 2.1
At4g24200.1 68417.m03474 expressed protein hypothetical protein ... 27 3.6
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 26 4.8
At5g18590.2 68418.m02198 kelch repeat-containing protein identic... 26 6.3
At5g18590.1 68418.m02197 kelch repeat-containing protein identic... 26 6.3
At3g18830.1 68416.m02391 mannitol transporter, putative similar ... 26 6.3
At2g16120.1 68415.m01848 mannitol transporter, putative similar ... 26 6.3
At5g63180.1 68418.m07932 pectate lyase family protein similar to... 25 8.3
At5g23730.1 68418.m02782 transducin family protein / WD-40 repea... 25 8.3
At4g27910.1 68417.m04006 PHD finger protein-related / SET domain... 25 8.3
At3g24880.1 68416.m03120 expressed protein 25 8.3
At3g24870.1 68416.m03119 expressed protein 25 8.3
>At1g65150.2 68414.m07387 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 296
Score = 30.7 bits (66), Expect = 0.22
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = -2
Query: 193 LAGECVGLPPFISESPNWMLLRHGVGDQWRNGS 95
LA E PPF S + NW L+ H G++ NGS
Sbjct: 29 LANEKYESPPFSSGAHNWRLVVHPKGNEADNGS 61
>At1g65150.1 68414.m07386 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 296
Score = 30.7 bits (66), Expect = 0.22
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = -2
Query: 193 LAGECVGLPPFISESPNWMLLRHGVGDQWRNGS 95
LA E PPF S + NW L+ H G++ NGS
Sbjct: 29 LANEKYESPPFSSGAHNWRLVVHPKGNEADNGS 61
>At2g47980.1 68415.m06004 expressed protein
Length = 1098
Score = 28.3 bits (60), Expect = 1.2
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +2
Query: 158 NERRKSYALSRQDDERKRLFDTQS 229
N RR+ R + ERKRLFD QS
Sbjct: 1030 NVRRRGRPRKRPETERKRLFDEQS 1053
>At2g29780.1 68415.m03618 kelch repeat-containing F-box family
protein similar to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 398
Score = 27.9 bits (59), Expect = 1.6
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = -3
Query: 171 FRRSFQNHQTGCYCGMVSVINGETGPLL 88
F ++ N T +C +++I GETGP+L
Sbjct: 345 FGATYNNPVTDTWCIEMALIKGETGPIL 372
>At3g20660.1 68416.m02615 organic cation transporter family protein
similar to organic cation transporter OCT3 [Rattus
norvegicus] GI:3273722, organic cation transporter 3
[Mus musculus] GI:4454795; contains Pfam profile
PF00083: major facilitator superfamily protein
Length = 534
Score = 27.5 bits (58), Expect = 2.1
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -2
Query: 166 PFISESPNWMLLRHGVGD 113
PFISESP W L+R V +
Sbjct: 266 PFISESPRWYLVRGKVDE 283
>At4g24200.1 68417.m03474 expressed protein hypothetical protein -
Arabidopsis thaliana,PIR2:T06677
Length = 1000
Score = 26.6 bits (56), Expect = 3.6
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +3
Query: 156 EMNGGSPTHSPAKMMNGSAYSTPSRGEREPAEH*GNPITG 275
E++ G P+HS ++ ++ + + S+ R+ H G+P G
Sbjct: 491 EVDSGEPSHSSSEELSDESGQSGSQYSRDDDVHTGSPSKG 530
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 26.2 bits (55), Expect = 4.8
Identities = 17/32 (53%), Positives = 20/32 (62%)
Frame = -2
Query: 226 LGVE*ALPFIILAGECVGLPPFISESPNWMLL 131
LG+E LP +I E + PP ISESP MLL
Sbjct: 231 LGLEHVLPAVI---ERIPPPPGISESPLRMLL 259
>At5g18590.2 68418.m02198 kelch repeat-containing protein identical
to RanGAP1 interacting protein (GI:21950739)
[Arabidopsis thaliana]; similar to Tip elongation
aberrant protein 1 (Cell polarity protein tea1)
(SP:P87061) [Schizosaccharomyces pombe]; contains Pfam
PF01344: Kelch motif (5 repeats)
Length = 708
Score = 25.8 bits (54), Expect = 6.3
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = -1
Query: 245 WLPLTSTGCRISASVHHL 192
WLPL TG R A HH+
Sbjct: 226 WLPLNCTGTRPCARSHHV 243
>At5g18590.1 68418.m02197 kelch repeat-containing protein identical
to RanGAP1 interacting protein (GI:21950739)
[Arabidopsis thaliana]; similar to Tip elongation
aberrant protein 1 (Cell polarity protein tea1)
(SP:P87061) [Schizosaccharomyces pombe]; contains Pfam
PF01344: Kelch motif (5 repeats)
Length = 708
Score = 25.8 bits (54), Expect = 6.3
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = -1
Query: 245 WLPLTSTGCRISASVHHL 192
WLPL TG R A HH+
Sbjct: 226 WLPLNCTGTRPCARSHHV 243
>At3g18830.1 68416.m02391 mannitol transporter, putative similar to
mannitol transporter [Apium graveolens var. dulce]
GI:12004316; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 539
Score = 25.8 bits (54), Expect = 6.3
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = -2
Query: 211 ALPFIILAGECVGLPPFISESPNWMLLRHGVGDQWR 104
A+P +ILA + +P ESP W++++ +GD R
Sbjct: 202 AVPSVILAIGVLAMP----ESPRWLVMQGRLGDAKR 233
>At2g16120.1 68415.m01848 mannitol transporter, putative similar to
mannitol transporter [Apium graveolens var. dulce]
GI:12004316; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 511
Score = 25.8 bits (54), Expect = 6.3
Identities = 15/41 (36%), Positives = 25/41 (60%)
Frame = -2
Query: 226 LGVE*ALPFIILAGECVGLPPFISESPNWMLLRHGVGDQWR 104
LGV A+P + LA + +P ESP W++L+ +GD ++
Sbjct: 188 LGVG-AVPSVFLAIGVLAMP----ESPRWLVLQGRLGDAFK 223
>At5g63180.1 68418.m07932 pectate lyase family protein similar to
pectate lyase GP:14289169 from [Salix gilgiana]
Length = 432
Score = 25.4 bits (53), Expect = 8.3
Identities = 15/45 (33%), Positives = 19/45 (42%)
Frame = +3
Query: 147 GDSEMNGGSPTHSPAKMMNGSAYSTPSRGEREPAEH*GNPITGDG 281
GD +NG T+S A S+YS S P+ H G G
Sbjct: 378 GDLLLNGAFFTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASG 422
>At5g23730.1 68418.m02782 transducin family protein / WD-40 repeat
family protein contains similarity to photomorphogenesis
repressor protein; contains Pfam PF00400: WD domain,
G-beta repeat (5 copies)
Length = 243
Score = 25.4 bits (53), Expect = 8.3
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -2
Query: 109 WRNGSIARCLSEKSRVSV 56
WRNG++ C SE +RV V
Sbjct: 165 WRNGALFGCGSENNRVFV 182
>At4g27910.1 68417.m04006 PHD finger protein-related / SET
domain-containing protein (TX4) nearly identical over
285 amino acids to trithorax 4 [Arabidopsis thaliana]
GI:16118405; contains Pfam profiles PF00856: SET domain,
PF00855: PWWP domain; identical to cDNA trithorax 4
(TX4) partial cds GI:16118404
Length = 1027
Score = 25.4 bits (53), Expect = 8.3
Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = +1
Query: 22 TRRPRHTDTSA*HSLETFRTGSEQWTRFSI--DHRHH 126
TR PRH ++A +L TFR E+ FS + HH
Sbjct: 842 TRGPRHHASAAIQTLNTFRHVPEEPKSFSSFRERLHH 878
>At3g24880.1 68416.m03120 expressed protein
Length = 1957
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +2
Query: 212 LFDTQSR*EGASRTLRQPNHR*RIQVDERANE 307
LFD++++ R LR PN + R ER+++
Sbjct: 137 LFDSENKSVEGERNLRHPNRQNRTSESERSSK 168
>At3g24870.1 68416.m03119 expressed protein
Length = 1841
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +2
Query: 212 LFDTQSR*EGASRTLRQPNHR*RIQVDERANE 307
LFD++++ R LR PN + R ER+++
Sbjct: 132 LFDSENKSVEGERNLRHPNRQNRTSESERSSK 163
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,638,217
Number of Sequences: 28952
Number of extensions: 158110
Number of successful extensions: 437
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 422
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 437
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 340508912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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