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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brS-0002
         (700 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC16E8.17c |||succinate-CoA ligase alpha subunit|Schizosacchar...   283   1e-77
SPBC1703.07 |||ATP citrate synthase subunit 1 |Schizosaccharomyc...    44   3e-05
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce...    29   0.85 
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|...    27   2.0  
SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc...    27   3.4  
SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha...    26   4.5  
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces...    25   7.9  
SPBC582.06c |mcp6|hrs1, mug3|meiosis specific coiled-coil protei...    25   7.9  
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy...    25   7.9  

>SPAC16E8.17c |||succinate-CoA ligase alpha
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 331

 Score =  283 bits (695), Expect = 1e-77
 Identities = 136/216 (62%), Positives = 162/216 (75%)
 Frame = +2

Query: 50  LSKFKDGLKLGNVRFASGNPYAETRKNLILTSETKVIVQGFTGKQGTFHSQQALDYGTKV 229
           L +F    +L N    S + Y +T  NL++ S+TKVI QGFTGKQGTFH+Q A+DYGTKV
Sbjct: 12  LRRFSSSSQLKN----SKSLYEQTIPNLMINSDTKVIFQGFTGKQGTFHAQHAMDYGTKV 67

Query: 230 VGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVIYVPPPGXXXXXXXXXXXXMPLIVC 409
           VGG +PKKAGT HLGKPVFGT++EA   T A AS ++VPPP             +PLIV 
Sbjct: 68  VGGTNPKKAGTTHLGKPVFGTIEEAMKETKADASAVFVPPPLAAGAIEEAIAAEVPLIVA 127

Query: 410 ITEGVPQHDMVRVKHALLRQNKSRLVGPNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRS 589
           ITEG+PQHDM+RV   L  Q+KSRLVGPNCPGII P +CKIGIMP+ +HK GCIG+VSRS
Sbjct: 128 ITEGIPQHDMLRVSDILKTQSKSRLVGPNCPGIIRPGQCKIGIMPSHIHKPGCIGIVSRS 187

Query: 590 GTLTYEACHQTTITGLGQTLCVGIGGDPFNGTDFID 697
           GTLTYEA +QTT T LGQ+L +GIGGDPF GT+FID
Sbjct: 188 GTLTYEAVNQTTQTDLGQSLVIGIGGDPFPGTNFID 223


>SPBC1703.07 |||ATP citrate synthase subunit 1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 615

 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 45/160 (28%), Positives = 69/160 (43%), Gaps = 13/160 (8%)
 Frame = +2

Query: 257 GTEHLGKPVFGTVKEAKAGTGATASVIYVPPPGXXXXXXXXXXXXMPLIVCIT---EGVP 427
           GT+ +  PV+ T++EA         V+                   P I CI    EGVP
Sbjct: 64  GTKEILLPVYRTIEEA-CTKHPEVDVVVNFASSRSAYASTMELMEFPQIRCIAIIAEGVP 122

Query: 428 QHDMVRVKHALLRQNKSRLV--GPNCPGIIAPEKCKIG--------IMPAAVHKRGCIGV 577
           +    R +  L+   +  +V  GP   G I P   KIG        I+ + +++ G +  
Sbjct: 123 ER---RAREILVTSKEKNVVIIGPATVGGIKPGCFKIGNTGGMMDNIVASKLYRPGSVAY 179

Query: 578 VSRSGTLTYEACHQTTITGLGQTLCVGIGGDPFNGTDFID 697
           VS+SG ++ E  +  + T  G    + IGGD + GT FID
Sbjct: 180 VSKSGGMSNELNNIISHTTDGVYEGIAIGGDRYPGTTFID 219


>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 28.7 bits (61), Expect = 0.85
 Identities = 16/50 (32%), Positives = 22/50 (44%)
 Frame = -1

Query: 529 FTLLRSNDTRTVRTNQSRLVLSQEGMFYSHHIMLWHTFCNAHNEGHFSLN 380
           +TLL  N T  +R +QS +     G  + H    W T  N  N  + S N
Sbjct: 186 YTLLLQNTTVVIRRDQSVVYTGDVGTNFFHSPTKWITHSNVSNGYYTSRN 235


>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
           subunit|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1752

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
 Frame = +2

Query: 107 PYAETRKNLILTSETKVIVQGFTGKQGTFHSQQALDYGTKVVG-GVSPKKAGTEHLGKPV 283
           PY+  R NL +TS        F G +   H  Q+ +   ++    + PK+  +    KPV
Sbjct: 470 PYSTFRLNLSVTSPYNA---DFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPV 526

Query: 284 FGTVKEAKAG 313
            G V++  AG
Sbjct: 527 MGIVQDTLAG 536


>SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 849

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 14/44 (31%), Positives = 24/44 (54%)
 Frame = -1

Query: 265 FCTSLLW*HSSNNFSTIVKGLLAVEGTLLSSETLNNHFCFTCKD 134
           FCTS ++   S   + I+KG+LA     + +++   HF  TC +
Sbjct: 252 FCTSEVFRCLSKR-AEILKGVLASNVIAMQTDSYTRHFLSTCSN 294


>SPCC1235.05c |fft2||fun thirty related protein
           Fft2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1284

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 10/34 (29%), Positives = 20/34 (58%)
 Frame = -2

Query: 318 PVPAFASLTVPNTGLPRCSVPAFFGDTPPTTLVP 217
           P+P++ + ++PN G+P  ++P      P T  +P
Sbjct: 124 PLPSYNTASLPNAGIP-AAMPGMPSGYPGTVPIP 156


>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 3131

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/69 (18%), Positives = 32/69 (46%)
 Frame = +2

Query: 8    FTFNNSTMAMPVRVLSKFKDGLKLGNVRFASGNPYAETRKNLILTSETKVIVQGFTGKQG 187
            +  NN+     V+V   F+  +KLG V        ++T++N +++     ++  F   + 
Sbjct: 801  YKLNNTPSEQSVKVSHFFEFNMKLGEVTLILCREDSQTKRNSMVSVNFAKLLLSFNQFED 860

Query: 188  TFHSQQALD 214
              H + +++
Sbjct: 861  NSHLRMSIN 869


>SPBC582.06c |mcp6|hrs1, mug3|meiosis specific coiled-coil protein
           Mcp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 327

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -2

Query: 93  NRTLPSFSPSLNLERTRTGIAIVELLNVNL 4
           NR  PS+S SLN   T   ++ VE +N +L
Sbjct: 297 NRAPPSYSFSLNCSHTYQPVSCVEPVNHDL 326


>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1583

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 3/65 (4%)
 Frame = -1

Query: 313 ACLCLFDCTKHRLTKMFCTSLLW*HS---SNNFSTIVKGLLAVEGTLLSSETLNNHFCFT 143
           A L L     +++  +F  SL   H+     NF+ I+  + ++      S+   NHF F 
Sbjct: 632 AALDLISLIVNKVLALFDLSLFEKHNIPAPTNFNDIISFIPSITRLNELSQVSFNHFYFL 691

Query: 142 CKDQI 128
           CK  I
Sbjct: 692 CKGDI 696


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,898,905
Number of Sequences: 5004
Number of extensions: 59204
Number of successful extensions: 159
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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