Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2486
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 253 2e-68
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 135 5e-33
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 130 2e-31
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 123 2e-29
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 110 2e-25
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 102 6e-23
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 89 5e-19
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 46 4e-06
SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|ch... 30 0.40
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 27 3.8
SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ... 26 6.6
SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pom... 25 8.7
SPBC4.05 |mlo2||zinc finger protein Mlo2|Schizosaccharomyces pom... 25 8.7
SPBC713.05 |||WD repeat protein, human MAPK organizer 1 |Schizos... 25 8.7
SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|ch... 25 8.7
SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 25 8.7
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 253 bits (619), Expect = 2e-68
Identities = 112/180 (62%), Positives = 145/180 (80%)
Frame = +3
Query: 102 MARRYDTRTTIFSPEGRLYQVEYAMEAISHAGTSLGILATDGILLAAERRNTNKLLDEVF 281
M+R YD+RTTIFSPEGRLYQVEYA+EAI+HAG +LGI+A DGI+LAAE++ T+KLL++
Sbjct: 1 MSRSYDSRTTIFSPEGRLYQVEYALEAINHAGVALGIVAKDGIVLAAEKKVTSKLLEQEE 60
Query: 282 FSEKIYKLNDDMVCSVAGITSDANVLTNELRLIAQRYLLQYGESIPCEQLVSWLCDVKQA 461
+EK+Y + D+M+C+VAG+T+DAN+L N R + Q+YL + E +PCEQLV +CD+KQ
Sbjct: 61 SAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQG 120
Query: 462 YTQYGGKRPFGVSILYMGWDKHYGYQLYQSDPSGNYGGWKATCIGNNSAAAVSSLKQEYK 641
YTQYGG RPFGVS LY GWD GYQL+QS+PSGNYG W+A IG NS + S ++QEYK
Sbjct: 121 YTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQANSIGGNSTSVQSLMRQEYK 180
Score = 27.9 bits (59), Expect = 1.6
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +1
Query: 631 RSTRENETTLAEAQALAIKVLNKTLDMTKPDSQK 732
R +++ L EA A+A+K L+KTLD +K
Sbjct: 176 RQEYKDDINLDEASAMAVKFLSKTLDSNSLTHEK 209
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 135 bits (327), Expect = 5e-33
Identities = 77/180 (42%), Positives = 106/180 (58%), Gaps = 1/180 (0%)
Frame = +3
Query: 102 MARRYDTRTTIFSPEGRLYQVEYAMEAISHAGTSLGILATDGILLAAERRNTNKLLDEVF 281
M +Y T FSP G+L Q+EYA+ A++ TS+GI ATDG++LA E++ T++L
Sbjct: 1 MTDKYAFSLTTFSPNGKLVQIEYALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIGAS 60
Query: 282 FSEKIYKLNDDMVCSVAGITSDANVLTNELRLIAQ-RYLLQYGESIPCEQLVSWLCDVKQ 458
EK+ + D+ +G+ D VL ++ R +AQ Y Y E P + LV + V Q
Sbjct: 61 L-EKVCAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQ 119
Query: 459 AYTQYGGKRPFGVSILYMGWDKHYGYQLYQSDPSGNYGGWKATCIGNNSAAAVSSLKQEY 638
TQ GG RPFGVS+L G D+ G LYQ DPSG Y WKAT IG +S AA + L++ Y
Sbjct: 120 ESTQSGGVRPFGVSLLVAGMDEK-GPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRY 178
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 130 bits (315), Expect = 2e-31
Identities = 67/177 (37%), Positives = 106/177 (59%), Gaps = 1/177 (0%)
Frame = +3
Query: 114 YDTRTTIFSPEGRLYQVEYAMEAISHAGTSLGILATDGILLAAERRNTNKLLDEVFFSEK 293
YD ++FSP+GRL QVEY EA+ T++ + + I++ ER+N KL + F +K
Sbjct: 4 YDRALSVFSPDGRLLQVEYGQEAVRRGTTAIALRGNECIVIGVERKNVPKLQNVSNF-QK 62
Query: 294 IYKLNDDMVCSVAGITSDANVLTNELRLIAQRYLLQYGESIPCEQLVSWLCDVKQAYTQY 473
I +++ + + AG+ +DA +L ++ R+ AQ + L + + E L ++ V+Q YTQ
Sbjct: 63 IAMVDNHVCLAFAGLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQS 122
Query: 474 GGKRPFGVSILYMGWDKHYGY-QLYQSDPSGNYGGWKATCIGNNSAAAVSSLKQEYK 641
GG RPFGVS L G+D ++YQ++P+G Y WKAT IG S AA L++ +K
Sbjct: 123 GGVRPFGVSTLIAGFDVGDNTPRVYQTEPAGIYNAWKATAIGRASKAAREYLEKNWK 179
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 123 bits (297), Expect = 2e-29
Identities = 67/181 (37%), Positives = 105/181 (58%), Gaps = 6/181 (3%)
Frame = +3
Query: 114 YDTRTTIFSPEGRLYQVEYAMEAISHAGTSLGILATDGILLAAERRNTNKLLDEVFFSEK 293
YD FSPEGRL+QVEYA+EAI T++G+ D ++L E+R T+ L+ E EK
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLM-ESHSVEK 66
Query: 294 IYKLNDDMVCSVAGITSDANVLTNELRLIAQRYLLQYGESIPCEQLVSWLCDVKQAYTQ- 470
+++++ + C+++G+T+DA + R+ Q + Y E E +CD+ + +
Sbjct: 67 LFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEG 126
Query: 471 YGGK-----RPFGVSILYMGWDKHYGYQLYQSDPSGNYGGWKATCIGNNSAAAVSSLKQE 635
G+ RPFGV++L G D+H G QLY S+PSG Y ++A IG+ S A S L +E
Sbjct: 127 EDGEERIMSRPFGVALLIAGIDEH-GPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKE 185
Query: 636 Y 638
+
Sbjct: 186 F 186
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 110 bits (264), Expect = 2e-25
Identities = 57/180 (31%), Positives = 98/180 (54%), Gaps = 1/180 (0%)
Frame = +3
Query: 105 ARRYDTRTTIFSPEGRLYQVEYAMEAISHAG-TSLGILATDGILLAAERRNTNKLLDEVF 281
AR +D T+FSPEGRLYQVEYA +A ++AG TS+G+ + + ++++ +KL+D
Sbjct: 4 ARGFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLIDAST 63
Query: 282 FSEKIYKLNDDMVCSVAGITSDANVLTNELRLIAQRYLLQYGESIPCEQLVSWLCDVKQA 461
++ + + C + G +DA + R A + + G +PC+ L + ++ Q
Sbjct: 64 VKH-VFPITKGIGCVMTGSIADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIAQV 122
Query: 462 YTQYGGKRPFGVSILYMGWDKHYGYQLYQSDPSGNYGGWKATCIGNNSAAAVSSLKQEYK 641
TQ RP GV++ + D G L++ DP+G Y G+KAT G ++ L++ +K
Sbjct: 123 STQRAAMRPLGVAMTLVAVDDEIGPSLFKLDPAGFYIGYKATSAGPKQTETINWLEKRFK 182
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 102 bits (244), Expect = 6e-23
Identities = 56/168 (33%), Positives = 97/168 (57%)
Frame = +3
Query: 111 RYDTRTTIFSPEGRLYQVEYAMEAISHAGTSLGILATDGILLAAERRNTNKLLDEVFFSE 290
+YD T +SP+GRL+QVEYA+EAI ++G+++ +L A +RN +L + +
Sbjct: 5 QYDGDATTWSPQGRLHQVEYALEAIKQGSATVGLVSKTHAVLVALKRNAEELSS---YQK 61
Query: 291 KIYKLNDDMVCSVAGITSDANVLTNELRLIAQRYLLQYGESIPCEQLVSWLCDVKQAYTQ 470
K+ +++D + ++AG+ DA VL+N ++ A + IP +L+S + + Q TQ
Sbjct: 62 KLIRIDDHIGIAIAGLAPDARVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQ 121
Query: 471 YGGKRPFGVSILYMGWDKHYGYQLYQSDPSGNYGGWKATCIGNNSAAA 614
G+RP+GV L +G+D+ G L + PSG + T +G+ S +A
Sbjct: 122 EYGRRPYGVGFLVIGYDES-GPHLLEFQPSGLVLEYLGTSMGSRSQSA 168
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 89.4 bits (212), Expect = 5e-19
Identities = 53/166 (31%), Positives = 82/166 (49%)
Frame = +3
Query: 135 FSPEGRLYQVEYAMEAISHAGTSLGILATDGILLAAERRNTNKLLDEVFFSEKIYKLNDD 314
FSP+GRL+Q EYA +A+ +A T +GI DG++LA E+ T+KLL + + +I ++
Sbjct: 14 FSPDGRLFQAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLL-KPRVNNRIGSVDRH 72
Query: 315 MVCSVAGITSDANVLTNELRLIAQRYLLQYGESIPCEQLVSWLCDVKQAYTQYGGKRPFG 494
+ + G D + R A + YG IP + L + Q +T Y RPFG
Sbjct: 73 IGIATTGFIPDGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFG 132
Query: 495 VSILYMGWDKHYGYQLYQSDPSGNYGGWKATCIGNNSAAAVSSLKQ 632
V +D G LY +P+G Y G+ G A + L++
Sbjct: 133 VMSFVATYDSE-GPHLYMVEPNGVYWGYNGAAAGKGRQVARNELEK 177
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 46.4 bits (105), Expect = 4e-06
Identities = 40/145 (27%), Positives = 64/145 (44%), Gaps = 2/145 (1%)
Frame = +3
Query: 183 ISHAGTSLGILATDGILLAAERRNTNKLLDEVFFSEKIYKLNDDMVCSVAGITSDANVLT 362
+ + GT++ I +LA + R+ N F +++++ DD+V +G +DA L
Sbjct: 10 VQNGGTTVAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADALAL- 68
Query: 363 NELRLIAQRYLLQYGESIPCEQLVSWLCDVKQAYTQYGGKR--PFGVSILYMGWDKHYGY 536
++ I QR L + S C V+ T GKR P+ V G DK
Sbjct: 69 --VKRIQQRIDLYHDNHERKMSAQSCACMVR---TLLYGKRFFPYYVYTTVAGIDKEGKG 123
Query: 537 QLYQSDPSGNYGGWKATCIGNNSAA 611
++Y DP G+Y + C SAA
Sbjct: 124 EIYSFDPVGSYE--REWCRAGGSAA 146
>SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 642
Score = 29.9 bits (64), Expect = 0.40
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = -1
Query: 606 HYCYRCMLLSTPHSFHWDQTGRADNHSAYPSPYT 505
HYC +C + P S+H R N + P+T
Sbjct: 400 HYCLKCFQVKPPRSYHCGACKRCINRYDHHCPWT 433
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 26.6 bits (56), Expect = 3.8
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 213 LATDGILLAAERRNTNKLLDEVFFSE 290
L + GIL +R+NT L+ V++SE
Sbjct: 6 LLSKGILSIIKRKNTGNLIPHVYYSE 31
>SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster
type |Schizosaccharomyces pombe|chr 2|||Manual
Length = 827
Score = 25.8 bits (54), Expect = 6.6
Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 1/61 (1%)
Frame = -3
Query: 370 NSFVKTLASDVIPATEQTMSSLSL*IFSEKNTSSRSLLVFLRSA-AKRIPSVAKIPSDVP 194
NSF TL +D +P SL +F + T SR L F+R + PS + +P
Sbjct: 382 NSFDFTLENDQVPPRLIASECTSLEVFIQHITLSRILSHFVRKVYPVKSPSDSHCKVSLP 441
Query: 193 A 191
A
Sbjct: 442 A 442
>SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1188
Score = 25.4 bits (53), Expect = 8.7
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = -2
Query: 410 FAILQQVSLSDQS*FVCQNVSIRCYTSD*TDHVIIELVDF 291
FA++ V L D V QN+ + +TS HV+ ++DF
Sbjct: 898 FAMVWLVDLEDN---VEQNIRVPIFTSSKPAHVLQNMIDF 934
>SPBC4.05 |mlo2||zinc finger protein Mlo2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 329
Score = 25.4 bits (53), Expect = 8.7
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = -2
Query: 341 CYTSD*TDHVIIELVDFFRKEHF 273
CY+ + H +LVD F K HF
Sbjct: 63 CYSCSISCHADHDLVDLFNKRHF 85
>SPBC713.05 |||WD repeat protein, human MAPK organizer 1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.4 bits (53), Expect = 8.7
Identities = 24/103 (23%), Positives = 43/103 (41%), Gaps = 3/103 (2%)
Frame = +3
Query: 96 EKMARRYDTRTTIFSPEGRLYQVEYAMEAISHAGTSLGILATDGILLAAERRNTNKLLDE 275
+ R +D R+ FSP L + ++ +I A + +TDG L + R L
Sbjct: 119 DSKVRLWDCRSNSFSPIQVLADAKDSVSSIDIAEHLIVTGSTDGTLRTYDIRKGT--LSS 176
Query: 276 VFFSEKIYKLNDDMVCS---VAGITSDANVLTNELRLIAQRYL 395
+FS I + S ++ + S ++L E I + Y+
Sbjct: 177 DYFSHPITSVKTSKSASFSLISSLNSSIHLLDQETGKILKSYI 219
>SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1374
Score = 25.4 bits (53), Expect = 8.7
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 3/39 (7%)
Frame = +3
Query: 366 ELRLIAQRYLLQYGESIPCEQLVS---WLCDVKQAYTQY 473
EL + RYLLQ+G S EQ + WL + + T Y
Sbjct: 728 ELLKKSTRYLLQFGLSTSLEQQIPTPYWLAPLNLSCTDY 766
>SPCC553.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 349
Score = 25.4 bits (53), Expect = 8.7
Identities = 20/62 (32%), Positives = 29/62 (46%)
Frame = -3
Query: 448 SQSQETNCSQGIDSPYCSRYL*AISLNSFVKTLASDVIPATEQTMSSLSL*IFSEKNTSS 269
+QSQ+ N +G S S + SL S + +S P+T + SS S S + SS
Sbjct: 103 AQSQQFNIVEGAASSSSSSSSSSSSLVSSTTSSSSSATPSTTSSSSSSSSSSSSSSSKSS 162
Query: 268 RS 263
S
Sbjct: 163 SS 164
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,333,018
Number of Sequences: 5004
Number of extensions: 74182
Number of successful extensions: 201
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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