Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-2477
         (450 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   166   2e-42
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   103   2e-23
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    98   5e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    95   4e-21
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom...    27   1.3  
SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy...    27   1.3  
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        27   1.8  
SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po...    25   4.1  
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce...    25   5.4  
SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos...    25   7.1  
SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schiz...    25   7.1  
SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces pombe...    25   7.1  
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc...    24   9.4  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  166 bits (403), Expect = 2e-42
 Identities = 76/102 (74%), Positives = 87/102 (85%)
 Frame = +1

Query: 145 SDLQLERIGVYYNEADDGKRFVPRAVLVDLEPGTMDAIKSSTYGALFRPDNYVFGQSGAG 324
           S+ Q ER+ VY+NEA  GK +VPRAVLVDLEPGTMDA+KS  +G LFRPDN ++GQSGAG
Sbjct: 40  SEAQHERLNVYFNEAAGGK-YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAG 98

Query: 325 NNWAKGHYTEGAELVESVLDVVRKEAEGCDCLQGFQLTHSFG 450
           N WAKGHYTEGAEL ++VLDVVR+EAE CD LQGFQLTHS G
Sbjct: 99  NIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLG 140



 Score = 69.7 bits (163), Expect = 2e-13
 Identities = 26/38 (68%), Positives = 32/38 (84%)
 Frame = +3

Query: 27  MREIVHLQTGQCGNQIGSKFWEIISDEHGIDTTGHYHG 140
           MREIVH+Q GQCGNQ+G+ FW  I+DEHG+D+ G YHG
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHG 38


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  103 bits (246), Expect = 2e-23
 Identities = 44/94 (46%), Positives = 65/94 (69%)
 Frame = +1

Query: 169 GVYYNEADDGKRFVPRAVLVDLEPGTMDAIKSSTYGALFRPDNYVFGQSGAGNNWAKGHY 348
           G +++E   GK FVPR++ VDLEP  +D +++  Y  LF P+  V G+  A NN+A+GHY
Sbjct: 54  GTFFSETGQGK-FVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112

Query: 349 TEGAELVESVLDVVRKEAEGCDCLQGFQLTHSFG 450
           T G E+++SVL+ +R+ A+ C  LQGF + HSFG
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFG 146



 Score = 38.7 bits (86), Expect = 4e-04
 Identities = 16/34 (47%), Positives = 21/34 (61%)
 Frame = +3

Query: 27  MREIVHLQTGQCGNQIGSKFWEIISDEHGIDTTG 128
           MRE++ +  GQ G QIG+  WE+   EHGI   G
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG 34


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 98.3 bits (234), Expect = 5e-22
 Identities = 42/92 (45%), Positives = 62/92 (67%)
 Frame = +1

Query: 175 YYNEADDGKRFVPRAVLVDLEPGTMDAIKSSTYGALFRPDNYVFGQSGAGNNWAKGHYTE 354
           +++E   GK +VPR++ VDLEP  +D +++  Y  LF P+  + G+  A NN+A+GHYT 
Sbjct: 52  FFSETGQGK-YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTV 110

Query: 355 GAELVESVLDVVRKEAEGCDCLQGFQLTHSFG 450
           G ELV+ V D +R+ A+ C  LQGF + HSFG
Sbjct: 111 GKELVDEVTDKIRRIADNCSGLQGFLVFHSFG 142



 Score = 42.3 bits (95), Expect = 3e-05
 Identities = 17/35 (48%), Positives = 22/35 (62%)
 Frame = +3

Query: 27  MREIVHLQTGQCGNQIGSKFWEIISDEHGIDTTGH 131
           MREI+ +  GQ G QIG+  WE+   EHGI   G+
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGY 35


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 95.1 bits (226), Expect = 4e-21
 Identities = 42/98 (42%), Positives = 69/98 (70%), Gaps = 2/98 (2%)
 Frame = +1

Query: 157 LERIGVYYNEADDGKRFVPRAVLVDLEPGTMDAIKSSTYGALFRPDNYVFGQS--GAGNN 330
           ++R  V++ ++DD  R++PRA+L+DLEP  ++ I S TYG+L+ P+N +  ++  GAGNN
Sbjct: 45  VDRKDVFFYQSDD-TRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNN 103

Query: 331 WAKGHYTEGAELVESVLDVVRKEAEGCDCLQGFQLTHS 444
           WA G Y+    + E ++D++ +EA+G D L+GF L HS
Sbjct: 104 WANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHS 140



 Score = 50.4 bits (115), Expect = 1e-07
 Identities = 21/33 (63%), Positives = 25/33 (75%)
 Frame = +3

Query: 30  REIVHLQTGQCGNQIGSKFWEIISDEHGIDTTG 128
           REI+ LQ GQCGNQIGS+FW+ +  EHGI   G
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDG 35


>SPAC1296.04 |mug65||spore wall assembly protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 27.1 bits (57), Expect = 1.3
 Identities = 9/22 (40%), Positives = 13/22 (59%), Gaps = 1/22 (4%)
 Frame = +2

Query: 173 FTTTKLMTASASCPAPFW-WTW 235
           F  + + T +A+CP P W W W
Sbjct: 46  FVASPVSTKTATCPDPSWEWAW 67


>SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 436

 Score = 27.1 bits (57), Expect = 1.3
 Identities = 13/30 (43%), Positives = 16/30 (53%)
 Frame = +2

Query: 212 PAPFWWTWSLGPWTPSSPRRTEPCSGRTTT 301
           P  F+ T SLG WTP++P    P   R  T
Sbjct: 403 PMDFYATNSLGNWTPNNP-ENRPLPNRKKT 431


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 26.6 bits (56), Expect = 1.8
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = +1

Query: 334 AKGHYTEGAELVESVLDVVRKEAEGCDCLQ 423
           A+GH   G ELV +  D +RK++E    L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 334

 Score = 25.4 bits (53), Expect = 4.1
 Identities = 14/43 (32%), Positives = 24/43 (55%)
 Frame = -1

Query: 327 IPCPALSKHVVVRPEQGSVRRGLDGVHGPRLQVHQNGAGHEAL 199
           +P  A ++H++ +PE   ++RG+  V+  R  V    A  EAL
Sbjct: 217 LPLNAHTRHIIGKPEFQKMKRGIVIVNTARGAVMDEAALVEAL 259


>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2104

 Score = 25.0 bits (52), Expect = 5.4
 Identities = 13/40 (32%), Positives = 19/40 (47%)
 Frame = +1

Query: 286  RPDNYVFGQSGAGNNWAKGHYTEGAELVESVLDVVRKEAE 405
            R  NY+ G+       A+G  T  A+L   + D V K+ E
Sbjct: 1656 RSKNYLTGRLQEVEELARGFQTTNADLQNELADAVVKQKE 1695


>SPBC16G5.15c |fkh2||fork head transcription factor Fkh2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 642

 Score = 24.6 bits (51), Expect = 7.1
 Identities = 11/36 (30%), Positives = 17/36 (47%)
 Frame = +2

Query: 119 HDWTLPRXRATCSSRESVFTTTKLMTASASCPAPFW 226
           HD+ L    A+ + + +  +     T   S PAPFW
Sbjct: 406 HDYALDANSASQTKKAAFGSPIGSSTYPTSSPAPFW 441


>SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 526

 Score = 24.6 bits (51), Expect = 7.1
 Identities = 11/36 (30%), Positives = 18/36 (50%)
 Frame = +1

Query: 247 MDAIKSSTYGALFRPDNYVFGQSGAGNNWAKGHYTE 354
           M  I+ +    L  P NY+FG++  G     G+ T+
Sbjct: 109 MSNIRKAQVKILKNPGNYIFGRTEYGKRTYSGNSTK 144


>SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 377

 Score = 24.6 bits (51), Expect = 7.1
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = +2

Query: 326 ITGLKVTTQKALSWWNPCWT 385
           I G+K   Q++ SWWN  W+
Sbjct: 35  INGIK-KDQQSSSWWNSLWS 53


>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
            Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1958

 Score = 24.2 bits (50), Expect = 9.4
 Identities = 12/29 (41%), Positives = 19/29 (65%)
 Frame = -2

Query: 134  VMSSRVDPVLVRDYFPELRSYLIATLSGL 48
            +   RV+P+LV+D F   R +  A++SGL
Sbjct: 1596 ITDDRVEPLLVKDTF---REFAEASVSGL 1621


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,805,011
Number of Sequences: 5004
Number of extensions: 34519
Number of successful extensions: 114
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 166231220
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)