Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2474
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 241 9e-65
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 177 9e-46
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 74 2e-14
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 49 7e-07
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 42 8e-05
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 32 0.091
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 29 0.48
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 28 1.5
SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|c... 28 1.5
SPAC23H3.09c |gly1||threonine aldolase |Schizosaccharomyces pomb... 26 4.5
SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc... 26 4.5
SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 26 6.0
SPAC4F10.02 |||aminopeptidase |Schizosaccharomyces pombe|chr 1||... 25 7.9
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 25 7.9
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb... 25 7.9
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 241 bits (589), Expect = 9e-65
Identities = 115/208 (55%), Positives = 142/208 (68%), Gaps = 2/208 (0%)
Frame = +1
Query: 28 KTWYHWMENIRDWCISRQLWWGHRIPAYKVSLP--AAHQHKEEELWVSGHTQEEALAKAV 201
+ + WMENI+DWCISRQLWWGHRIPAY V+L + E WV+G T E+A KA
Sbjct: 501 REFIRWMENIQDWCISRQLWWGHRIPAYFVNLADEPSQDRSEGRYWVTGRTLEQAEEKAK 560
Query: 202 SKFGVSASEVTLRQDDDVLDTWFSSGLFTFSIFGWPDETDDLKAFYPGTLLETGHDILFF 381
+ F TL QD+DVLDTWFSSGL+ FS GWP +T D + FYP TL+ETG DILFF
Sbjct: 561 AAF--PGKSFTLEQDEDVLDTWFSSGLWPFSTLGWPKDTSDYENFYPTTLMETGWDILFF 618
Query: 382 WVARMVFFGQRLMGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDPVDVVXGVTPRGVAPP 561
W+ARMV G +L GK+PFK V+ H +VRDA GRKMSKSLGNV+DP+DV+ G++ + +
Sbjct: 619 WIARMVMLGLKLTGKIPFKRVFCHALVRDAQGRKMSKSLGNVVDPIDVIEGISLQALHDK 678
Query: 562 TGRLELGPRGEVEKAKQGQKPDYSHXHP 645
L R EVEKAK+GQ+ Y P
Sbjct: 679 LLVGNLDSR-EVEKAKKGQRLSYPKGIP 705
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 177 bits (432), Expect = 9e-46
Identities = 92/199 (46%), Positives = 119/199 (59%), Gaps = 1/199 (0%)
Frame = +1
Query: 28 KTWYHWMENIRDWCISRQLWWGHRIPAYKVSLPAAHQHKEEELWVSGHTQEEALAKAVSK 207
K W W + + DWCISRQ+WWGH+IP +K+ EE+ W++ E+AL +V K
Sbjct: 425 KEWDSWFKKMGDWCISRQIWWGHQIPVWKIL--------EEDKWIAAPNYEKALQLSVGK 476
Query: 208 FGVSASEVTLRQDDDVLDTWFSSGLFTFSIFGWPDETDDLKAFYPGTLLETGHDILFFWV 387
++ QD DVLDTWFSS L S FGWP D P +E+G DILFFW+
Sbjct: 477 --------SVSQDSDVLDTWFSSALLPLSAFGWPKSKD----IQPLPFIESGQDILFFWI 524
Query: 388 ARMVFFGQRLMGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDPVDVVXGVTPRGVAPPTG 567
ARM + +LPFKE+ LHP+VRD+ GRKMSKSLGNVIDP+D++ GVT +
Sbjct: 525 ARMALLCKYFSNELPFKEIILHPLVRDSEGRKMSKSLGNVIDPMDIINGVTLENMKKALL 584
Query: 568 RLELGPRGEVEK-AKQGQK 621
L P EV K +KQ +K
Sbjct: 585 EGNL-PISEVHKSSKQMEK 602
>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1064
Score = 73.7 bits (173), Expect = 2e-14
Identities = 49/173 (28%), Positives = 77/173 (44%), Gaps = 7/173 (4%)
Frame = +1
Query: 19 VHEKTWYHWMENIRDWCISRQLWWGHRIPAY------KVSLPAAHQHKEEELWVSGHTQE 180
+ +K + +W++N RDW ISR +WG IP + +V + + EE VS T
Sbjct: 436 IRDKRFANWLKNARDWNISRNRYWGTPIPLWVSDDYEEVVCIGSIKELEELSGVSNITDI 495
Query: 181 EALAKAVSKFGVSASEVTLRQDDDVLDTWFSSGLFTFSIFGWPDET-DDLKAFYPGTLLE 357
+ + TL + +V D WF SG ++ +P E ++ K +P +
Sbjct: 496 HRDSIDHITIPSKKGKGTLHRVSEVFDCWFESGSMPYASRHYPFERIEEFKHGFPADFIS 555
Query: 358 TGHDILFFWVARMVFFGQRLMGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDP 516
G D W + G L K P+K V + +V G+KMSK L N +P
Sbjct: 556 EGVDQTRGWFYTLTVLGTLLFDKAPYKNVIVSGLVMAEDGKKMSKRLKNYPEP 608
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 973
Score = 48.8 bits (111), Expect = 7e-07
Identities = 51/183 (27%), Positives = 76/183 (41%), Gaps = 14/183 (7%)
Frame = +1
Query: 61 DWCISRQLWWGHRIPAY-----KVSLPAAHQ--HKEEELWVSG------HTQEEALAKAV 201
+WCISRQ WG IP K+ L + E++ V G T+ A+ V
Sbjct: 480 EWCISRQRAWGLPIPVLYEKGTKIPLLTVKSVSYIIEKMEVEGVDSWFNDTENNGHAQWV 539
Query: 202 SKFGVSASEVTLRQDDDVLDTWFSSGLFTFSIFGWPDETDDL-KAFYPGTLLETGHDILF 378
+ + + + LD WF SG +I P + L G+ G +
Sbjct: 540 HPDYRNKEYI---RGTETLDVWFDSGTSWTTIA--PRKNKPLIDLCLEGSDQHRG----W 590
Query: 379 FWVARMVFFGQRLMGKLPFKEVYLHPMVRDAHGRKMSKSLGNVIDPVDVVXGVTPRGVAP 558
F + + + + PF ++ H + D G+K SKSLGNV DP DV+ G +G
Sbjct: 591 FQSLLLTYTAYQSKPEAPFSTLFTHGFIFDERGQKQSKSLGNVTDPEDVINGKLLKGKKL 650
Query: 559 PTG 567
P G
Sbjct: 651 PYG 653
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 41.9 bits (94), Expect = 8e-05
Identities = 38/127 (29%), Positives = 60/127 (47%), Gaps = 6/127 (4%)
Frame = +1
Query: 265 WFSSGLFTFSIFGWP--DETDDLKAFYPGTLLETGHDILFFWVARMVFFGQRLMGK-LPF 435
W + + S+ G+P +E L A +P + G DI+ F +++ LM LP
Sbjct: 254 WLDALINYISVIGYPWLNEKSSLSAGWPANMHVIGKDIIRF---HCIYWPAFLMAAGLPL 310
Query: 436 KEVYLHPMVRDAHGRKMSKSLGNVIDPVDVV--XGV-TPRGVAPPTGRLELGPRGEVEKA 606
E L + KMSKSLGNV+DP ++ GV T R GRL ++E+
Sbjct: 311 PEKILVHSHWTMNKVKMSKSLGNVVDPFWLIEKYGVDTIRYYLLKRGRLTSDSNFDIEEL 370
Query: 607 KQGQKPD 627
++ ++ D
Sbjct: 371 EKDEEHD 377
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 31.9 bits (69), Expect = 0.091
Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Frame = -3
Query: 578 SSSLPVGGATPRGVTPXTTSTGSMTLPSDLDIFRP*A-SRTIGWRYTSLKGSFPMRR 411
SS LP T +P T++ +T P+D+ P + S T GW + G PMRR
Sbjct: 820 SSILPTVPRTTSYTSPYATTSSHIT-PADVHPLPPPSTSTTAGWNDAPMLGQLPMRR 875
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 29.5 bits (63), Expect = 0.48
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +1
Query: 55 IRDWCISRQLWWGHRIPAYKVSLPAAHQHKEEEL 156
++DW ISRQ +WG IP A E EL
Sbjct: 427 LKDWLISRQRFWGTPIPMVHCETCGAVPVPESEL 460
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1778
Score = 27.9 bits (59), Expect = 1.5
Identities = 14/36 (38%), Positives = 18/36 (50%)
Frame = -3
Query: 623 GFCPCFAFSTSPRGPSSSLPVGGATPRGVTPXTTST 516
G P F ST+P G + S ATP G + T +T
Sbjct: 240 GSTPAFGASTTPFGQNLSGTTNNATPFGTSNATNTT 275
>SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 297
Score = 27.9 bits (59), Expect = 1.5
Identities = 11/44 (25%), Positives = 22/44 (50%)
Frame = +1
Query: 19 VHEKTWYHWMENIRDWCISRQLWWGHRIPAYKVSLPAAHQHKEE 150
+H K + + ++ + W + W PAY+ S PAA + + +
Sbjct: 76 LHSKVYDLYRDSPKIWLRRERQWLQRHSPAYRFSTPAAKRPRRQ 119
>SPAC23H3.09c |gly1||threonine aldolase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 376
Score = 26.2 bits (55), Expect = 4.5
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 544 RGVAPPTGRLELGPRGEVEKAKQGQK 621
+G+A P G + +GPR + KAK +K
Sbjct: 226 KGLAAPVGSIIVGPRDFIAKAKWFRK 251
>SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 849
Score = 26.2 bits (55), Expect = 4.5
Identities = 17/58 (29%), Positives = 25/58 (43%)
Frame = +1
Query: 40 HWMENIRDWCISRQLWWGHRIPAYKVSLPAAHQHKEEELWVSGHTQEEALAKAVSKFG 213
H + R+ I W GH + SLP + + EEL E +A+ S+FG
Sbjct: 368 HKLRAFRELLIHFPKWRGHVVLIQITSLPVGNNYSNEEL----KKTENLVAQINSEFG 421
>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 754
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +1
Query: 475 GRKMSKSLGNVIDPVDVVXGVTPR 546
G+KMSKSL N I +++ TPR
Sbjct: 406 GQKMSKSLKNFITIKEILKKFTPR 429
>SPAC4F10.02 |||aminopeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 467
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +1
Query: 445 YLHPMVRDAHGRKMSKSLGNVIDPVDVV 528
Y HP++ ++SKSL IDP +V
Sbjct: 201 YHHPVLLSLLANEISKSLETTIDPSKIV 228
>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1040
Score = 25.4 bits (53), Expect = 7.9
Identities = 14/52 (26%), Positives = 28/52 (53%), Gaps = 2/52 (3%)
Frame = +1
Query: 103 PAY--KVSLPAAHQHKEEELWVSGHTQEEALAKAVSKFGVSASEVTLRQDDD 252
P+Y ++LP H H +++ + HT E+ K + G S + ++L ++D
Sbjct: 244 PSYTNSLALPPFHHHNNYKMFNTTHTLED--DKFLQGKGTSNNPISLSDEED 293
>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 782
Score = 25.4 bits (53), Expect = 7.9
Identities = 10/22 (45%), Positives = 14/22 (63%), Gaps = 1/22 (4%)
Frame = +1
Query: 43 WM-ENIRDWCISRQLWWGHRIP 105
W+ E +R CI+R L WG +P
Sbjct: 443 WLKEGLRPRCITRDLKWGTPVP 464
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,663,824
Number of Sequences: 5004
Number of extensions: 54241
Number of successful extensions: 216
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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