Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2397
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 95 1e-20
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 36 0.006
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 36 0.008
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 1.2
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 2.9
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 27 3.8
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.6
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa... 26 6.6
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 26 6.6
SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 25 8.7
SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase Snf21|... 25 8.7
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 8.7
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 94.7 bits (225), Expect = 1e-20
Identities = 62/177 (35%), Positives = 90/177 (50%), Gaps = 11/177 (6%)
Frame = +2
Query: 197 DKGLKTSEDARFYALSRKF-KPFSNXGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQXD 373
+ GL ++A +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ + +
Sbjct: 89 EAGLVMKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGE 147
Query: 374 MHGETPYEIMFGPDICGPGTKXVHVIFSY-------XGKNHLIKKDIRCKDDVYTHLYTL 532
M Y IMFGPD CG + VH IF + + HL + T+LYTL
Sbjct: 148 MSNSIDYRIMFGPDKCGVNDR-VHFIFKHKNPLTGEYSEKHLDSRPASLLKPGITNLYTL 206
Query: 533 IVKPDNTYEVLIDNE---KS*IWRPXSRLGLPXA*KIXDPGAXKPEDWDDKPTXXXP 694
IVKPD T+EV I+ + + ++ LP +I DP KP DW D+P P
Sbjct: 207 IVKPDQTFEVRINGDVVRQGSLFYDFIPPVLPPV-EIYDPEDIKPADWVDEPEIPDP 262
Score = 29.9 bits (64), Expect = 0.40
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = +2
Query: 632 IXDPGAXKPEDWDDK 676
I DP A KPEDWDD+
Sbjct: 297 IPDPEAQKPEDWDDE 311
Score = 27.1 bits (57), Expect = 2.9
Identities = 11/22 (50%), Positives = 13/22 (59%), Gaps = 2/22 (9%)
Frame = +1
Query: 691 PRXQKPEDWDK--PEHFPXPDA 750
P KP+DWD+ P P PDA
Sbjct: 262 PNAVKPDDWDEDAPRMIPDPDA 283
Score = 27.1 bits (57), Expect = 2.9
Identities = 11/17 (64%), Positives = 13/17 (76%), Gaps = 1/17 (5%)
Frame = +2
Query: 632 IXDPGAXKPEDW-DDKP 679
I DP A KPEDW +D+P
Sbjct: 278 IPDPDAVKPEDWLEDEP 294
Score = 26.2 bits (55), Expect = 5.0
Identities = 11/22 (50%), Positives = 14/22 (63%), Gaps = 2/22 (9%)
Frame = +1
Query: 691 PRXQKPEDW--DKPEHFPXPDA 750
P KPEDW D+P + P P+A
Sbjct: 281 PDAVKPEDWLEDEPLYIPDPEA 302
Score = 25.8 bits (54), Expect = 6.6
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = +1
Query: 691 PRXQKPEDWDKPE 729
P QKPEDWD E
Sbjct: 300 PEAQKPEDWDDEE 312
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 35.9 bits (79), Expect = 0.006
Identities = 26/72 (36%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
Frame = -1
Query: 231 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFXGCSLYTQLLSHE-SSGNFSSKNTSQFIE 55
N SSE F S+S SL P VN + S + ++ L S + S+ NF+S ++S +
Sbjct: 475 NYTSSEAF---STSSSLVVIPPVNSSLVTSSTSFTKFSSLSSSQLSTENFTSASSSLSLT 531
Query: 54 DNASKLTTTSTT 19
+ S L+T STT
Sbjct: 532 NAKSSLSTPSTT 543
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 35.5 bits (78), Expect = 0.008
Identities = 48/180 (26%), Positives = 77/180 (42%), Gaps = 8/180 (4%)
Frame = +2
Query: 77 FEEKFPDD---SWESNWVYSEHPXKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL-S 244
F E+F + +W S W + K+ G + L S D+ GL T + + + L S
Sbjct: 33 FSEQFTSEDITTWRSRWRAPVN--KDLGVWDLVEAPG-SHLRDEYGLITLKSNKPHILIS 89
Query: 245 RKFKPFSNXGK--PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQXDMHGETPYEIMFGPDI 418
P + P+V+ F VK + CG Y+ + + ++ E P I FG
Sbjct: 90 NLENPTTRQSSSVPIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKK 148
Query: 419 CGPGTKXVHVIFSYXGK--NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKS*IW 592
CG I SY GK HL + T +YTL+++ NT V+I ++S ++
Sbjct: 149 CGMFDYISLSIISYDGKVSCHLYDAPPSGLVEGRTSMYTLLLQ--NT-TVVIRRDQSVVY 205
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 28.3 bits (60), Expect = 1.2
Identities = 15/44 (34%), Positives = 19/44 (43%)
Frame = -1
Query: 162 NLNFPNSFXGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTT 31
N N F + + S +SGNFS T Q DN S +T
Sbjct: 575 NSNSQRPFSTVPSESNVFSRNASGNFSMSQTHQPTTDNTSSFST 618
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.1 bits (57), Expect = 2.9
Identities = 33/137 (24%), Positives = 59/137 (43%)
Frame = -1
Query: 423 PQMSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLR 244
P S I S SP S SS S + S S + + + T+ + + + R
Sbjct: 336 PTSSSSTISSSSSSPSSSSFSSTTSSS---KSSSSFSSTVSSSSSTSSSTLTSSSSSSSR 392
Query: 243 ERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFXGCSLYTQLLSHESSGNFSSKNTSQ 64
+ + SS + SSS S + V+ ++F S ++ SH SS + SS ++
Sbjct: 393 PASSSSHSSSLSSHKSSSSSKSSSAPVS----SAFYHNSTSSRSSSHSSSHSLSSLSSKP 448
Query: 63 FIEDNASKLTTTSTTAF 13
+ ++S L T+S+ +
Sbjct: 449 ILTASSSSLLTSSSHTY 465
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +2
Query: 83 EKFPDDSWESNWVYSEHPXKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 226
+ F +D + +W EH K ++ + + FS+ DD+ + SE +
Sbjct: 49 QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95
>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 536
Score = 25.8 bits (54), Expect = 6.6
Identities = 27/123 (21%), Positives = 53/123 (43%), Gaps = 2/123 (1%)
Frame = -1
Query: 363 SSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLRERA*NLASSEVFKPLSSSG- 187
+SL S P S S + T ++ +PS + + A ++SS PLSSS
Sbjct: 12 TSLSSSASSSIPASSSSAAASTSLSSSSVIPSSSSSMLSSSSATAISSSSSSSPLSSSSF 71
Query: 186 -SLKNFPAVNLNFPNSFXGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTTTSTTAFI 10
S + + +S S + L+ SS +S +++ ++S ++S+++ +
Sbjct: 72 TSPASSSFITSLVSSSSQQSSSSSASLTSSSSATLTSSSSASPTSSSSSHALSSSSSSLV 131
Query: 9 FDS 1
S
Sbjct: 132 ASS 134
>SPBC215.15 |sec13||COPII-coated vesicle component
Sec13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.8 bits (54), Expect = 6.6
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +2
Query: 80 EEKFPDDSWESNWVYS 127
EEKFPD +W +W S
Sbjct: 251 EEKFPDIAWRVSWSLS 266
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 25.8 bits (54), Expect = 6.6
Identities = 18/58 (31%), Positives = 23/58 (39%)
Frame = +2
Query: 209 KTSEDARFYALSRKFKPFSNXGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQXDMHG 382
KT Y +SRK P L + F K DC G L+V D L+ +G
Sbjct: 952 KTIPRDLLYEVSRKINPLHQASTRLAIAFPDKRLLQYDC--GKLQVLDRLLKDLVSNG 1007
>SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1315
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/31 (38%), Positives = 14/31 (45%)
Frame = -2
Query: 125 CTPNCFPTNHQETSPQRIHHNLSKIMPAS*Q 33
C P FP N+QE H+ I AS Q
Sbjct: 791 CKPESFPANYQEVLDYYTHNGFRVIACASKQ 821
>SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase
Snf21|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1199
Score = 25.4 bits (53), Expect = 8.7
Identities = 14/55 (25%), Positives = 27/55 (49%)
Frame = -3
Query: 379 VHVXLLQFAVKDLKVSASTVNVLFMFDSELDDQGFTXITERFELTGESIEPRIFR 215
V++ LL L ++ T + + +FDS+ + +R G++ E RI+R
Sbjct: 805 VNLFLLSTRAGGLGLNLQTADTVIIFDSDWNPHQDLQAQDRAHRIGQTKEVRIYR 859
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 25.4 bits (53), Expect = 8.7
Identities = 30/145 (20%), Positives = 58/145 (40%), Gaps = 1/145 (0%)
Frame = -1
Query: 432 VPGPQMSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGL 253
+P S P+ S SP S SS S S S + ++ ++ +PS + +
Sbjct: 518 IPSTSSSTPSS-SIISSPMTSVLSSSSSIPTSSSSDFSSSITTISSGISSSSIPSTFSSV 576
Query: 252 NLRERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSF-XGCSLYTQLLSHESSGNFSSK 76
+ + + S +SSS + F + + + P+S S + +LS + S
Sbjct: 577 SSILSSSTSSPSSTSLSISSSSTSSTFSSASTSSPSSISSSISSSSTILSSPTPSTSSLM 636
Query: 75 NTSQFIEDNASKLTTTSTTAFIFDS 1
+S I +S + ++S + S
Sbjct: 637 ISSSSIISGSSSILSSSISTIPISS 661
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,993,038
Number of Sequences: 5004
Number of extensions: 60271
Number of successful extensions: 203
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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