Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2326
(400 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 217 4e-58
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 217 5e-58
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 217 5e-58
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 118 3e-28
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 117 6e-28
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 76 2e-15
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 53 1e-08
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 42 3e-05
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 42 3e-05
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 38 4e-04
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 38 4e-04
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 31 0.006
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 30 0.15
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 28 0.61
SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 27 0.81
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 27 1.4
SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharom... 26 2.5
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 26 2.5
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 25 5.7
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 24 7.6
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 24 10.0
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 24 10.0
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 217 bits (531), Expect = 4e-58
Identities = 103/124 (83%), Positives = 105/124 (84%)
Frame = +3
Query: 27 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVL 206
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKR I KFE EA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 207 DKLXAERERGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLIXXAGX 386
DKL AERERGITIDIALWKFET K VT IDAPGHRDF NMITGTS ADCAVLI G
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGT 120
Query: 387 AECE 398
E E
Sbjct: 121 GEFE 124
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 217 bits (530), Expect = 5e-58
Identities = 102/124 (82%), Positives = 105/124 (84%)
Frame = +3
Query: 27 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVL 206
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKR I KFE EA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 207 DKLXAERERGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLIXXAGX 386
DKL AERERGITIDIALWKFET K VT IDAPGHRDF NMITGTS ADCA+LI G
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGT 120
Query: 387 AECE 398
E E
Sbjct: 121 GEFE 124
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 217 bits (530), Expect = 5e-58
Identities = 102/124 (82%), Positives = 105/124 (84%)
Frame = +3
Query: 27 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVL 206
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKR I KFE EA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 207 DKLXAERERGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLIXXAGX 386
DKL AERERGITIDIALWKFET K VT IDAPGHRDF NMITGTS ADCA+LI G
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGT 120
Query: 387 AECE 398
E E
Sbjct: 121 GEFE 124
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 118 bits (285), Expect = 3e-28
Identities = 56/111 (50%), Positives = 71/111 (63%)
Frame = +3
Query: 39 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVLDKLX 218
K +++VV GHVDSGKST G ++++ G I+ R + K EA GKGSF YAW+LD
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 219 AERERGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLI 371
ER RG+T+D+A FE+ K DAPGHRDF MI G S AD AVL+
Sbjct: 235 EERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLV 285
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 117 bits (282), Expect = 6e-28
Identities = 55/120 (45%), Positives = 75/120 (62%)
Frame = +3
Query: 39 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVLDKLX 218
K H+NIV IGHVD+GKST G++++ G +DKR + K E EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 219 AERERGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLIXXAGXAECE 398
ERE+G T+++ FET + +DAPGH+ + NMI G S AD VL+ A E E
Sbjct: 296 EEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFE 355
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 75.8 bits (178), Expect = 2e-15
Identities = 43/116 (37%), Positives = 62/116 (53%)
Frame = +3
Query: 33 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVLDK 212
++K H+NI IGHVD GK+T T I KC ++G+ SF +DK
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC--------------LSDLGQASFMDYSQIDK 93
Query: 213 LXAERERGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLIXXA 380
E+ RGITI A ++ET+ + +D PGH D+ NMITG + D A+++ A
Sbjct: 94 APEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSA 149
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 53.2 bits (122), Expect = 1e-08
Identities = 36/107 (33%), Positives = 50/107 (46%)
Frame = +3
Query: 51 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVLDKLXAERE 230
N+ +I H+D+GK+T T ++Y G F + G V+D L AER+
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG---------FTSHFGNVDTGDT----VMDYLPAERQ 76
Query: 231 RGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLI 371
RGITI+ A F + ID PGH DF + + D AV I
Sbjct: 77 RGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAI 123
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 42.3 bits (95), Expect = 3e-05
Identities = 37/118 (31%), Positives = 52/118 (44%), Gaps = 3/118 (2%)
Frame = +3
Query: 27 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGID--KRXIXKF-EXEAQEMGKGSFKYA 197
MGK N+ VI HVD GKST T L+ K G I K +F + A E +G +
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKS 72
Query: 198 WVLDKLXAERERGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLI 371
+ L AE D+ + + V ID+PGH DF + D A+++
Sbjct: 73 TAI-SLFAEMTDDDMKDMK-EPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGALVV 128
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 42.3 bits (95), Expect = 3e-05
Identities = 37/118 (31%), Positives = 52/118 (44%), Gaps = 3/118 (2%)
Frame = +3
Query: 27 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGID--KRXIXKF-EXEAQEMGKGSFKYA 197
MGK N+ VI HVD GKST T L+ K G I K +F + A E +G +
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKS 72
Query: 198 WVLDKLXAERERGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLI 371
+ L AE D+ + + V ID+PGH DF + D A+++
Sbjct: 73 TAI-SLFAEMTDDDMKDMK-EPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGALVV 128
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 38.3 bits (85), Expect = 4e-04
Identities = 39/128 (30%), Positives = 54/128 (42%), Gaps = 18/128 (14%)
Frame = +3
Query: 51 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVLDKLXAERE 230
NI + H+DSGK+T T ++Y G I + E+ +G +D + ERE
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI---------KDIHEV-RGKDNVGAKMDFMELERE 110
Query: 231 RGITIDIA----LW-------------KFETSKXY-VTXIDAPGHRDFFXNMITGTSXAD 356
+GITI A W K + K Y + ID PGH DF + D
Sbjct: 111 KGITIQSAATHCTWERTVDQIEANEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLD 170
Query: 357 CAVLIXXA 380
AVL+ A
Sbjct: 171 GAVLVLCA 178
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 38.3 bits (85), Expect = 4e-04
Identities = 31/114 (27%), Positives = 49/114 (42%), Gaps = 4/114 (3%)
Frame = +3
Query: 51 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVLDKLXAERE 230
N VI H+D GKST + ++ G I++ + F LDKL ER
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNF-----------RNQF-----LDKLEVERR 103
Query: 231 RGITIDI----ALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLIXXA 380
RGIT+ ++ + + ID PGH DF ++ + + +L+ A
Sbjct: 104 RGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDA 157
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 31.1 bits (67), Expect(2) = 0.006
Identities = 19/68 (27%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
Frame = +3
Query: 174 GKGSFKYAWVLDKLXAERERGITIDIALWK--FETSKXYVTXIDAPGHRDFFXNMITGTS 347
GK + A+ + + GIT I + F+ ++T +D PGH F G +
Sbjct: 183 GKTTLLDAFRKSTIASTEHGGITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMRKRGAN 242
Query: 348 XADCAVLI 371
AD VL+
Sbjct: 243 IADIVVLV 250
Score = 22.2 bits (45), Expect(2) = 0.006
Identities = 7/13 (53%), Positives = 11/13 (84%)
Frame = +3
Query: 54 IVVIGHVDSGKST 92
+ ++GHVD GK+T
Sbjct: 174 VTLMGHVDHGKTT 186
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 29.9 bits (64), Expect = 0.15
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +3
Query: 282 YVTXIDAPGHRDFFXNMITGTSXADCAVLIXXAGXAEC 395
+V+ +D PGH M+ G + D A+L+ AG C
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLL-IAGNESC 145
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 27.9 bits (59), Expect = 0.61
Identities = 24/104 (23%), Positives = 43/104 (41%)
Frame = +3
Query: 60 VIGHVDSGKSTTTGHLIYKCGGIDKRXIXKFEXEAQEMGKGSFKYAWVLDKLXAERERGI 239
++GHVD+GK+ +L + + + Q++G F + K ++G
Sbjct: 489 ILGHVDTGKTKLLDNL--RRSNVQEGEAGGI---TQQIGATYFPIESIKQKTKVVNKKG- 542
Query: 240 TIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLI 371
K + + + ID PGH F GTS + A+L+
Sbjct: 543 -------KLQYNIPGLLIIDTPGHESFTNLRSRGTSLCNIAILV 579
>SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 704
Score = 27.5 bits (58), Expect = 0.81
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -1
Query: 244 IVIPRSRSAFSLSNTQAYLKDPLPISWASFSNFXMXRLSIPPH 116
I IP+ A S+ N++ + +D LP + S +L +P H
Sbjct: 171 IPIPKKNPARSVCNSKLFNEDTLPAEFEEVSISPPVKLELPTH 213
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 26.6 bits (56), Expect = 1.4
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = -2
Query: 213 VCPIPKHI*RILYPFPGPXSRTSXWXVCQYLHICRSNDQWWWT 85
V +PK I I++ F P S S C+Y S+D W T
Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRT 73
>SPCC63.03 |||DNAJ domain protein, DNAJC11
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 642
Score = 25.8 bits (54), Expect = 2.5
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = -1
Query: 268 SNFQRAISIVIPRSRSAFSLSNTQAYLKDPLPISWAS 158
S F IS+ +P FSL+ ++ K LPI W S
Sbjct: 403 STFGVNISVGVPTGSITFSLNWSRLGQKISLPIMWCS 439
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 25.8 bits (54), Expect = 2.5
Identities = 26/121 (21%), Positives = 49/121 (40%), Gaps = 6/121 (4%)
Frame = +3
Query: 51 NIVVIGHVDSGKSTTTGHLIYKCGGIDKR---XIXKFEXEAQEMGKG-SFKYAWV--LDK 212
N ++ HVD GK+T L+ G I + + + E+ +G + K + + K
Sbjct: 21 NFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITRGITMKSSAISLFFK 80
Query: 213 LXAERERGITIDIALWKFETSKXYVTXIDAPGHRDFFXNMITGTSXADCAVLIXXAGXAE 392
+ ++ + K + ID+PGH DF + + + D A ++ A
Sbjct: 81 VISQNDE---------KRVEKDYLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEGV 131
Query: 393 C 395
C
Sbjct: 132 C 132
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 24.6 bits (51), Expect = 5.7
Identities = 9/29 (31%), Positives = 15/29 (51%)
Frame = +3
Query: 57 VVIGHVDSGKSTTTGHLIYKCGGIDKRXI 143
+V+GH GK+T +Y+ G + I
Sbjct: 45 IVLGHTGCGKTTQIPQFLYEAGWASQNGI 73
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 24.2 bits (50), Expect = 7.6
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +3
Query: 54 IVVIGHVDSGKSTTTGHLIYKC 119
IVV+G SGKST + +C
Sbjct: 115 IVVVGETGSGKSTQIPQFLNEC 136
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 23.8 bits (49), Expect = 10.0
Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = +3
Query: 33 KEKTHINIVVIGHVDSGKSTTTGHL-IYKCGGIDKRXIXKF 152
K+K ++++G DSGKST + + I G + I F
Sbjct: 30 KDKKIYKVLLLGASDSGKSTISKQIKILNKNGFSQEEIMTF 70
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 23.8 bits (49), Expect = 10.0
Identities = 8/23 (34%), Positives = 14/23 (60%)
Frame = +3
Query: 54 IVVIGHVDSGKSTTTGHLIYKCG 122
++V+G SGK+T +Y+ G
Sbjct: 511 LIVVGETGSGKTTQLAQFLYEDG 533
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,276,761
Number of Sequences: 5004
Number of extensions: 19573
Number of successful extensions: 72
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 64
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 71
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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