BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2258
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23H4.12 |alp13||Clr6 histone deacetylase complex subunit Alp... 56 7e-09
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 27 2.2
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 27 2.9
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 27 2.9
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 25 8.7
SPCC1235.14 |ght5||hexose transporter Ght5 |Schizosaccharomyces ... 25 8.7
>SPAC23H4.12 |alp13||Clr6 histone deacetylase complex subunit
Alp13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 337
Score = 55.6 bits (128), Expect = 7e-09
Identities = 28/72 (38%), Positives = 46/72 (63%)
Frame = +1
Query: 268 ERVLCYEPDPTKAKVLYDSKVLEVVESKDKRGRXTVEYLIHFQGWNSSWDRCVSEDFVLK 447
ERVLC+ +LY++K+++ + +G T YLIH++GW +SWD V +D +L+
Sbjct: 9 ERVLCFH-----GPLLYEAKIVDT----EMKGDVTT-YLIHYKGWKNSWDEWVEQDRILQ 58
Query: 448 DTEENRQLQRDL 483
TEEN + Q++L
Sbjct: 59 WTEENLKTQKEL 70
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 27.5 bits (58), Expect = 2.2
Identities = 20/63 (31%), Positives = 29/63 (46%)
Frame = -1
Query: 474 LQLSIFLCIFENEVFTDTAIPRRVPSLEMD*ILNSXTPSFVFALYDFKNLGIIKHLGFSR 295
L + L F N+ TA +R+PS + IL FV + +N G K +G +
Sbjct: 540 LDIRTHLVRFLNKFSIPTA--QRLPSSDCSKILKCLLDGFVRNVAHLQNDGSYKTIGGKQ 597
Query: 294 VWL 286
VWL
Sbjct: 598 VWL 600
>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 27.1 bits (57), Expect = 2.9
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Frame = -2
Query: 296 GSGS*HRTRSPSVNLYRSPRVETIVN*CLAQN*WELNKITPFNKCDG---*VCKILVKNK 126
G+G + P N Y +P + + + + L ++TPF K DG KIL+K K
Sbjct: 525 GAGLPSKGYDPGSNTYVAPSSVNVKDVAIDPHSKRLQRLTPFKKWDGKDMKGLKILIKAK 584
Query: 125 KK 120
K
Sbjct: 585 GK 586
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 27.1 bits (57), Expect = 2.9
Identities = 17/61 (27%), Positives = 26/61 (42%)
Frame = -3
Query: 712 IVXRRRTEXXSCQXKQTMNPCRPFRLRLAGYLSKSRRR*SHDGCXXXXXXXXRLYYHXSF 533
++ R R SC K+ + R +GY + +R SH+GC +Y H S
Sbjct: 1031 LMDRYRHPVVSCGRKRELI----LRALCSGYFTNVAKRDSHEGCYKTIVENAPVYMHPSG 1086
Query: 532 V 530
V
Sbjct: 1087 V 1087
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 25.4 bits (53), Expect = 8.7
Identities = 20/69 (28%), Positives = 28/69 (40%), Gaps = 5/69 (7%)
Frame = +1
Query: 157 PSHLLNGVILLSS--HQF-WARHQLTMVSTRGLRYKFTEGERVLCYEPD--PTKAKVLYD 321
PS + LL S H W RH + + L K T E V ++PD K+L D
Sbjct: 144 PSEDIKNDFLLDSLKHALHWDRHAKVVCTAIQLLVKVTGAEAVFAFQPDFFEQSLKLLKD 203
Query: 322 SKVLEVVES 348
+ + S
Sbjct: 204 YSYAQAISS 212
>SPCC1235.14 |ght5||hexose transporter Ght5 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 546
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 62 LESFGRPQPVNIARLLQIIVFFCF 133
LE FGR P+ I L Q I FF +
Sbjct: 322 LEFFGRRMPLIIGALWQSITFFIY 345
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,680,735
Number of Sequences: 5004
Number of extensions: 49391
Number of successful extensions: 124
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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