Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-2248
         (660 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1D7.01 |||prefoldin subunit 1 |Schizosaccharomyces pombe|chr...    39   7e-04
SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz...    33   0.028
SPBC1778.02 |rap1||telomere binding protein Rap1|Schizosaccharom...    30   0.34 
SPBC25H2.15 |||programmed cell death protein homolog|Schizosacch...    29   0.45 
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...    28   1.0  
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c...    27   2.4  
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch...    27   2.4  
SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15 |Sc...    27   3.2  
SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces...    26   4.2  
SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|...    26   4.2  
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch...    25   7.3  

>SPBC1D7.01 |||prefoldin subunit 1 |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 112

 Score = 38.7 bits (86), Expect = 7e-04
 Identities = 20/100 (20%), Positives = 52/100 (52%)
 Frame = +3

Query: 132 LQVLMIETKKTIENIDHQLAVLGRMQMHTNLTQREIDVLPPGTKTYESMARMFVQSDLEH 311
           +Q   +++++ +  I  Q+A   R    + L ++E++ +      Y S+ +MF++SDL  
Sbjct: 8   IQEKAVDSQQQLRTIMVQIAAKERALKISELAKKELEDVGEDKAVYTSLGKMFMKSDLAS 67

Query: 312 IKQNLRQKTNTLISRIDDLNNRKVCLNHTLNESESNIRDL 431
           ++ +L  +  +L   I+ L  ++     T + +E +++ +
Sbjct: 68  VRTHLDAEMASLREDIEALQKKQTYHETTASNAEQHLQKI 107


>SPBC3D6.04c |mad1||mitotic spindle checkpoint protein
           Mad1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 689

 Score = 33.5 bits (73), Expect = 0.028
 Identities = 20/90 (22%), Positives = 46/90 (51%)
 Frame = +3

Query: 201 RMQMHTNLTQREIDVLPPGTKTYESMARMFVQSDLEHIKQNLRQKTNTLISRIDDLNNRK 380
           R+Q   +L  +EID+L    K+Y+    +  + + +  K    ++   L+  +D+   + 
Sbjct: 382 RLQRQKSLATQEIDLLRENLKSYDDEEAILSEKNTDMKK---LERIEGLVKLVDEYKLKL 438

Query: 381 VCLNHTLNESESNIRDLIQQKRMKNENQPA 470
             +  +L+  E++    +Q++R KNE++ A
Sbjct: 439 ESMPVSLDVDETSDEVSLQKRRRKNEHKDA 468


>SPBC1778.02 |rap1||telomere binding protein
           Rap1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 693

 Score = 29.9 bits (64), Expect = 0.34
 Identities = 19/75 (25%), Positives = 34/75 (45%)
 Frame = +3

Query: 255 GTKTYESMARMFVQSDLEHIKQNLRQKTNTLISRIDDLNNRKVCLNHTLNESESNIRDLI 434
           GTK YE +AR + Q  LE  +Q+ +     L   + D +    C    + +  S+ +D  
Sbjct: 146 GTKVYEELARKYPQHSLESWRQHYKYMKKRL-PPVSDSDESNYC-QRIIVKPYSSQKDYT 203

Query: 435 QQKRMKNENQPAAES 479
           Q    +  + P ++S
Sbjct: 204 QSTHEQTLSSPISKS 218


>SPBC25H2.15 |||programmed cell death protein
           homolog|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 396

 Score = 29.5 bits (63), Expect = 0.45
 Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 5/42 (11%)
 Frame = +3

Query: 213 HTNLTQREIDVLPPGTKTY-----ESMARMFVQSDLEHIKQN 323
           H  LT   + V PP T+TY     E+   MF+  DLE++ QN
Sbjct: 202 HIALTTSGMSVHPPVTETYTYPVTEAFQGMFLGLDLEYVPQN 243


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1000

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 19/64 (29%), Positives = 36/64 (56%), Gaps = 3/64 (4%)
 Frame = +3

Query: 144 MIETKKTIENIDHQLAVLGR-MQMHTNLTQREIDVLPPGTKTYESMARMFVQ--SDLEHI 314
           +++  KTIEN+    +   R + +  +LT+   +VL P  K YE ++++  +  SDL  +
Sbjct: 686 LLKHSKTIENVSSNFSKKNRNVVVSESLTKSMEEVLTP-EKFYERLSKLLEEENSDLGEL 744

Query: 315 KQNL 326
           K +L
Sbjct: 745 KNHL 748


>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1687

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 15/62 (24%), Positives = 30/62 (48%)
 Frame = +3

Query: 234 EIDVLPPGTKTYESMARMFVQSDLEHIKQNLRQKTNTLISRIDDLNNRKVCLNHTLNESE 413
           E  +LP    +YE++  +F + D +H+ ++       LI +  D +     L H +N+  
Sbjct: 364 ESQILPCVLHSYETLTNVFHRDDSKHLYESAVDHGRRLIMKFSD-SELHTFLFHCVNKIV 422

Query: 414 SN 419
           +N
Sbjct: 423 AN 424


>SPCC162.08c |nup211||nuclear pore complex associated
            protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1837

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 24/118 (20%), Positives = 47/118 (39%), Gaps = 11/118 (9%)
 Frame = +3

Query: 108  ELKQAFVDLQVLMIETKKTIENIDHQLAVLGRMQMHTN-----------LTQREIDVLPP 254
            EL +++  LQ  +   K  ++N+  QL++       +N           L     D L  
Sbjct: 741  ELDESYKSLQEQLASKKIEVQNVSSQLSICNSQLEQSNHIVDNLKSENLLLTSVKDKLKA 800

Query: 255  GTKTYESMARMFVQSDLEHIKQNLRQKTNTLISRIDDLNNRKVCLNHTLNESESNIRD 428
                 ES     +Q D  H+K  +        + +D +N+R + L++ L  + S + +
Sbjct: 801  DLSNLESKLSS-LQQDNFHMKAQIESSNQEYTATVDSMNSRILELSNDLRVANSKLSE 857


>SPCC1183.01 |sec15|SPCC1672.13|exocyst complex subunit Sec15
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 785

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 28/133 (21%), Positives = 52/133 (39%), Gaps = 1/133 (0%)
 Frame = +3

Query: 75  TIIKMAKTVDLELKQAFVDLQVLMIETKKTIENIDHQLAVLGRMQMHTNLTQREIDVLP- 251
           T  ++ K V   L++    LQ + +E+   +   D     L  +    +L    +D+L  
Sbjct: 601 TTTQVRKDVSGYLQEMVTYLQTMYLESLAGLPKHDKSYVYLETLD---HLCTAMVDLLSD 657

Query: 252 PGTKTYESMARMFVQSDLEHIKQNLRQKTNTLISRIDDLNNRKVCLNHTLNESESNIRDL 431
           P  +   + A    + D+E+++    Q  +  I   D     + C N  L ++     D 
Sbjct: 658 PSIRKVTTAAAEGFKLDVEYLESFAAQVPDQSIVNADSFIELRQCANLLLGDNMEEYLDT 717

Query: 432 IQQKRMKNENQPA 470
            +  R  N  QPA
Sbjct: 718 DKFMRDFNRLQPA 730


>SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 324

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 31/151 (20%), Positives = 62/151 (41%), Gaps = 6/151 (3%)
 Frame = +3

Query: 42  RCPRR*FYFLTTIIKMAKTVDLELKQAFVDLQVLMIETKKTIENIDHQLAVLGRMQ---M 212
           RCP   +Y  TTI    K    ++++     +    E +  I N+  QL    + Q   +
Sbjct: 2   RCPLAYYYTQTTIPTQQKRKSKKMEELLSKQREECKELQSKITNLRKQLKEGNKKQKRAL 61

Query: 213 HTNLTQREIDVLPPGTKTYESMARMFVQSDLEHIKQNLRQKTNTLISRIDD---LNNRKV 383
              ++Q E D+    ++ + +  +   + D E  +       NTL+ +++D       K 
Sbjct: 62  QQKISQMEADL----SQKHATERQKLDKGDEETNETQQEDLLNTLLQQMEDTKITTAEKS 117

Query: 384 CLNHTLNESESNIRDLIQQKRMKNENQPAAE 476
            +  +LN  E+  +   Q K+ +N  +   E
Sbjct: 118 SVQSSLNTKENTPQ---QPKKSRNRQKERLE 145


>SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 401

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 16/74 (21%), Positives = 36/74 (48%), Gaps = 2/74 (2%)
 Frame = +3

Query: 261 KTYESMARMFVQSDL--EHIKQNLRQKTNTLISRIDDLNNRKVCLNHTLNESESNIRDLI 434
           KTYE + + +    L  + +K  L    NTL++ + +   +   L+  L  +  + +DL 
Sbjct: 294 KTYEEVEKAYKDLTLSPQDLKLGLESSVNTLLAGVQEQLKQYPDLDSILKAAYPDPKDLK 353

Query: 435 QQKRMKNENQPAAE 476
           +  + K +++  A+
Sbjct: 354 KAMKQKKQDKKNAK 367


>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 425

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = +3

Query: 330 QKTNTLISRIDDLNNRKVCLNHTLNE 407
           QKT + + R+ D N+R++CL  T  E
Sbjct: 92  QKTGSELFRLHDRNDREMCLAPTHEE 117


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,299,580
Number of Sequences: 5004
Number of extensions: 41798
Number of successful extensions: 113
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)