Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2237
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 139 4e-34
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 80 3e-16
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 77 3e-15
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 72 7e-14
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 58 2e-09
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 54 3e-08
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 50 2e-07
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 29 0.48
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 28 1.5
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 26 6.0
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 26 6.0
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 139 bits (336), Expect = 4e-34
Identities = 65/86 (75%), Positives = 67/86 (77%)
Frame = +1
Query: 301 PXXTEIVTNWDDMXKXWHHTFYNELRVAPXEHPXLLTEAPLNPXAXRXKMTQIMFETXNT 480
P IV NWDDM K WHHTFYNELRVAP EHP LLTEAPLNP + R KMTQI+FET N
Sbjct: 70 PIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNA 129
Query: 481 PXMYVXIQAVLSLYASGRTTGIXLXS 558
P YV IQAVLSLYASGRTTGI L S
Sbjct: 130 PAFYVAIQAVLSLYASGRTTGIVLDS 155
Score = 92.3 bits (219), Expect = 6e-20
Identities = 42/50 (84%), Positives = 43/50 (86%)
Frame = +3
Query: 165 DAPRAVFPSIVGRPRHQGVMVGXGQKDSYV*DEAQSKRGXLTLXYPXXHG 314
DAPRAVFPSIVGRPRH G+MVG GQKDSYV DEAQSKRG LTL YP HG
Sbjct: 25 DAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHG 74
Score = 76.6 bits (180), Expect = 3e-15
Identities = 34/46 (73%), Positives = 38/46 (82%)
Frame = +2
Query: 563 DGVSHTVPIYEGYALPHAXLRLXXGGPDLTDYLMKILTERXYSXTT 700
DGV+HTVPIYEGYALPHA +RL G DLTDYLMKIL ER Y+ +T
Sbjct: 157 DGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFST 202
Score = 44.4 bits (100), Expect = 2e-05
Identities = 18/22 (81%), Positives = 21/22 (95%)
Frame = +2
Query: 95 DEEVAALVVXNGSGMCKAGFAG 160
+EE+AALV+ NGSGMCKAGFAG
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAG 23
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 79.8 bits (188), Expect = 3e-16
Identities = 36/86 (41%), Positives = 54/86 (62%)
Frame = +1
Query: 301 PXXTEIVTNWDDMXKXWHHTFYNELRVAPXEHPXLLTEAPLNPXAXRXKMTQIMFETXNT 480
P I+ ++++M + W +TF+ +L++ P LLTE P+NP A R KM + MFE
Sbjct: 70 PMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGF 129
Query: 481 PXMYVXIQAVLSLYASGRTTGIXLXS 558
+YV IQAVLSLYA G ++G+ + S
Sbjct: 130 GGVYVAIQAVLSLYAQGLSSGVVVDS 155
Score = 43.6 bits (98), Expect = 3e-05
Identities = 19/43 (44%), Positives = 25/43 (58%)
Frame = +2
Query: 563 DGVSHTVPIYEGYALPHAXLRLXXGGPDLTDYLMKILTERXYS 691
DGV+H VP+YE L H RL G D T YL+ +L + Y+
Sbjct: 157 DGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYA 199
Score = 35.9 bits (79), Expect = 0.006
Identities = 22/50 (44%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Frame = +3
Query: 171 PRAVFPSIVGRP--RHQGVMVGXGQKDSYV*DEAQSKRGXLTLXYPXXHG 314
PR FPSIVGRP R + KD V DEA++ R L + YP +G
Sbjct: 25 PRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRSLLQVKYPMENG 74
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 76.6 bits (180), Expect = 3e-15
Identities = 36/85 (42%), Positives = 49/85 (57%), Gaps = 1/85 (1%)
Frame = +1
Query: 301 PXXTEIVTNWDDMXKXWHHTFYNE-LRVAPXEHPXLLTEAPLNPXAXRXKMTQIMFETXN 477
P I+ NW DM + W + + ++ L P EHP LLTE PL + K+ + +ET N
Sbjct: 74 PIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLN 133
Query: 478 TPXMYVXIQAVLSLYASGRTTGIXL 552
P + +Q VL+LYAS RTTGI L
Sbjct: 134 VPALSFSLQPVLALYASARTTGIVL 158
Score = 46.8 bits (106), Expect = 3e-06
Identities = 22/50 (44%), Positives = 30/50 (60%)
Frame = +3
Query: 165 DAPRAVFPSIVGRPRHQGVMVGXGQKDSYV*DEAQSKRGXLTLXYPXXHG 314
D P+ +FP+ VGR +H+ VM QKD +V EAQ+ RG L + P G
Sbjct: 29 DIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERG 78
Score = 44.8 bits (101), Expect = 1e-05
Identities = 17/33 (51%), Positives = 26/33 (78%)
Frame = +2
Query: 563 DGVSHTVPIYEGYALPHAXLRLXXGGPDLTDYL 661
DG++H+VPIY+G+++P A + GG D+TDYL
Sbjct: 162 DGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYL 194
Score = 26.2 bits (55), Expect = 4.5
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +2
Query: 113 LVVXNGSGMCKAGFAG 160
+ + NGSG KAGFAG
Sbjct: 12 ICIDNGSGFIKAGFAG 27
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 72.1 bits (169), Expect = 7e-14
Identities = 31/70 (44%), Positives = 42/70 (60%)
Frame = +1
Query: 319 VTNWDDMXKXWHHTFYNELRVAPXEHPXLLTEAPLNPXAXRXKMTQIMFETXNTPXMYVX 498
+ NWD M + W + + LR P +H LLTE PLNP R +IMFE+ N +Y+
Sbjct: 99 IENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIA 158
Query: 499 IQAVLSLYAS 528
+QAVL+L AS
Sbjct: 159 VQAVLALAAS 168
Score = 35.5 bits (78), Expect = 0.007
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +2
Query: 563 DGVSHTVPIYEGYALPHAXLRLXXGGPDLTDYLMKILTER 682
DGV+H +P+ EGY + + + G D+T ++ +L +R
Sbjct: 188 DGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDR 227
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 57.6 bits (133), Expect = 2e-09
Identities = 27/74 (36%), Positives = 37/74 (50%)
Frame = +1
Query: 319 VTNWDDMXKXWHHTFYNELRVAPXEHPXLLTEAPLNPXAXRXKMTQIMFETXNTPXMYVX 498
V NWD W + +L+ P EHP L+TE NP R K + MFE+ P Y+
Sbjct: 74 VENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCPATYLA 133
Query: 499 IQAVLSLYASGRTT 540
Q + +ASG+ T
Sbjct: 134 KQETCAAFASGKGT 147
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 53.6 bits (123), Expect = 3e-08
Identities = 30/96 (31%), Positives = 49/96 (51%), Gaps = 2/96 (2%)
Frame = +1
Query: 298 TPXXTEIVTNWDDMXKXWHHTFYNELRVAPXEHPXLLTEAPLNPXAXRXKMTQIMFETXN 477
+P +++NWD M + +TF +L + EHP +TE NP R MT+++FE N
Sbjct: 85 SPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYN 143
Query: 478 TPXMYVXIQAVLSLYASGR--TTGIXLXSXGRCLPH 579
P + I + S Y + + ++GI L + G H
Sbjct: 144 APSVAYGIDGLFSFYHNTKPSSSGIVL-NLGNAASH 178
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/36 (27%), Positives = 18/36 (50%)
Frame = +2
Query: 563 DGVSHTVPIYEGYALPHAXLRLXXGGPDLTDYLMKI 670
+ SH +P+ G + R+ GG + YL+K+
Sbjct: 174 NAASHVIPVLNGERILSEAKRISWGGSQSSSYLLKL 209
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 50.4 bits (115), Expect = 2e-07
Identities = 21/77 (27%), Positives = 40/77 (51%)
Frame = +1
Query: 316 IVTNWDDMXKXWHHTFYNELRVAPXEHPXLLTEAPLNPXAXRXKMTQIMFETXNTPXMYV 495
I+ +W+ W +L+V P E+ ++TE NP + R ++ + FE + P Y+
Sbjct: 76 IIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYL 135
Query: 496 XIQAVLSLYASGRTTGI 546
QAV +A+ ++T +
Sbjct: 136 TKQAVCVAFANSKSTAL 152
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 29.5 bits (63), Expect = 0.48
Identities = 12/40 (30%), Positives = 23/40 (57%)
Frame = +2
Query: 572 SHTVPIYEGYALPHAXLRLXXGGPDLTDYLMKILTERXYS 691
+H +P+ + A R+ GG LT+YL ++++ R Y+
Sbjct: 168 THIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYN 207
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 27.9 bits (59), Expect = 1.5
Identities = 10/29 (34%), Positives = 19/29 (65%)
Frame = +2
Query: 587 IYEGYALPHAXLRLXXGGPDLTDYLMKIL 673
+ EG +P++ +++ GG D+T MK+L
Sbjct: 308 VEEGVVVPNSRIKINYGGDDITLLFMKLL 336
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 25.8 bits (54), Expect = 6.0
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 3/38 (7%)
Frame = +1
Query: 91 VRRRSCRVGSXQWLRYVQG---RFRRSMMLLAPCSPRS 195
VRR++ +GS +R+V G R R+ L P SP +
Sbjct: 1100 VRRKNVNIGSSNSIRHVSGSTSRSTRTRSLSKPLSPEA 1137
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/36 (33%), Positives = 16/36 (44%)
Frame = +2
Query: 467 KHXTXPXCTXXSKPCSRCTRPVVPPXSXXTPADGVS 574
+H T P +K SR P+ PP S P +S
Sbjct: 162 EHLTPPSSFITAKQLSRLPTPLPPPSSSSLPTGTIS 197
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,340,337
Number of Sequences: 5004
Number of extensions: 39293
Number of successful extensions: 105
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 101
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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