Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2123
(699 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 282 3e-77
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 138 9e-34
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 123 2e-29
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 111 1e-25
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 89 7e-19
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 74 2e-14
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 70 3e-13
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 66 3e-12
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 3e-05
SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 29 0.64
SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.85
SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit S... 27 2.6
SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr 3|||M... 26 4.5
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 282 bits (692), Expect = 3e-77
Identities = 138/193 (71%), Positives = 150/193 (77%)
Frame = +3
Query: 33 GRDLTDYLMKILTERGSRSLPPPSGKSFVTSRRSCATSLLTSSRKWPPLHPAASLEKSYE 212
GRDLTDYLMKIL ERG + + L ++ ++SLEKSYE
Sbjct: 182 GRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYE 241
Query: 213 LPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSG 392
LPDGQVITIGNERFR PEALFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N V+SG
Sbjct: 242 LPDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSG 301
Query: 393 GTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAXLXTXQQMWISKQE 572
GTTMYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILA L T QQMWISKQE
Sbjct: 302 GTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQE 361
Query: 573 YDESGPFIVHRKC 611
YDESGP IV+RKC
Sbjct: 362 YDESGPGIVYRKC 374
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 138 bits (333), Expect = 9e-34
Identities = 71/195 (36%), Positives = 105/195 (53%), Gaps = 3/195 (1%)
Frame = +3
Query: 33 GRDLTDYLMKILTERGSRSLPPPSGKSFVTSRRSC---ATSLLTSSRKWPPLHPAASLEK 203
GRD+TDYL L + G + + + C A+ + W P
Sbjct: 187 GRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIH--- 243
Query: 204 SYELPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTV 383
+Y+LPD Q IT+G E F PE LF P +G EA G+H + SI+ D+D+R LY+N V
Sbjct: 244 TYQLPDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIV 303
Query: 384 LSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAXLXTXQQMWIS 563
LSGG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILA L T +++ I+
Sbjct: 304 LSGGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLIT 363
Query: 564 KQEYDESGPFIVHRK 608
+EY I R+
Sbjct: 364 SEEYKNDQNVIFRRR 378
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 123 bits (297), Expect = 2e-29
Identities = 71/197 (36%), Positives = 106/197 (53%), Gaps = 12/197 (6%)
Frame = +3
Query: 33 GRDLTDYLMKILTERGSRSLPPPSGKSFVTSRRSCATSLLTSSRKWPPLHPAASLEKSYE 212
GRD T YL+ +L +G ++ + L ++Y
Sbjct: 182 GRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYT 241
Query: 213 LPDGQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSG 392
LPDG+VI +G+ER+ CPE LFQP +G E G+ E +++I DVDIRK LY VLSG
Sbjct: 242 LPDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSG 301
Query: 393 GTTMYPGIADRMQKEITAL--------APSTM---KIKIIAPPERKYSVWIGGSILAXLX 539
G++MY G+ R++KEI L P+ + K+KI P R+++V+IGG++LA +
Sbjct: 302 GSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIM 361
Query: 540 T-XQQMWISKQEYDESG 587
MW+SK E++E G
Sbjct: 362 AQNDHMWVSKAEWEEYG 378
Score = 38.3 bits (85), Expect = 0.001
Identities = 16/30 (53%), Positives = 22/30 (73%)
Frame = +2
Query: 71 RARFSFTTTAEREIVRDIKEKLCYVALDFE 160
R ++F TA+ E VR++KEKLCYV+ D E
Sbjct: 195 RKGYAFNRTADFETVREMKEKLCYVSYDLE 224
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 111 bits (266), Expect = 1e-25
Identities = 60/151 (39%), Positives = 82/151 (54%), Gaps = 14/151 (9%)
Frame = +3
Query: 201 KSYELPDGQVITIGNERFRCPEALFQPSFLGMEAC---------GIHETTYNSIMKCDVD 353
K +E PDG G ERFR E LF PSF + G+HE Y SI+ CD +
Sbjct: 279 KPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSE 338
Query: 354 IRKDLYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKII-APPERKYSVWIGGSIL 527
+R L N V++GGT++ PG+++R+Q E+ LA S + + +VW GGSIL
Sbjct: 339 LRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSIL 398
Query: 528 AXLXTXQQMWISKQEYDESG---PFIVHRKC 611
A L Q +W+SKQEYDE G V ++C
Sbjct: 399 ASLDNFQHLWVSKQEYDEVGVDRALFVEKRC 429
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 88.6 bits (210), Expect = 7e-19
Identities = 44/97 (45%), Positives = 63/97 (64%), Gaps = 3/97 (3%)
Frame = +3
Query: 306 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 485
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384
Query: 486 P---ERKYSVWIGGSILAXLXTXQQMWISKQEYDESG 587
ER Y+ W+GGSIL+ L T Q+WIS+QEY+E G
Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHG 421
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 74.1 bits (174), Expect = 2e-14
Identities = 42/128 (32%), Positives = 62/128 (48%)
Frame = +3
Query: 225 QVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTM 404
QV+ + NERF PE LF PS + + GI E S+ +I+ L N V GG
Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCK 331
Query: 405 YPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAXLXTXQQMWISKQEYDES 584
+PG R+ E+ +LAP+ ++K+ P + W S + L I++ EY E
Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEH 390
Query: 585 GPFIVHRK 608
G I+ RK
Sbjct: 391 GANIMTRK 398
Score = 29.5 bits (63), Expect = 0.48
Identities = 11/23 (47%), Positives = 18/23 (78%)
Frame = +2
Query: 101 EREIVRDIKEKLCYVALDFEQEM 169
E IV +IKE +CYV+ +F+++M
Sbjct: 211 ETYIVNEIKESVCYVSQNFKEDM 233
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 70.1 bits (164), Expect = 3e-13
Identities = 54/210 (25%), Positives = 91/210 (43%), Gaps = 19/210 (9%)
Frame = +3
Query: 33 GRDLTDYLMKILTERGSRSLPPPSGKSFVTSRRSCATSLLTSSRKWPPL--HPAASLEKS 206
GRD+T ++ +L +R P S K+ + C +++ P L+ +
Sbjct: 213 GRDVTYFVQSLLRDRNE---PDSSLKTAERIKEECCYVCPDIVKEFSRFDREPDRYLKYA 269
Query: 207 YELPDGQVITI--GNERFRCPEALFQPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYAN 377
E G TI G ERF PE F P + + E N + +D+RK LY N
Sbjct: 270 SESITGHSTTIDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKN 329
Query: 378 TVLSGGTTMYPGIADRMQKEITALAPST--------------MKIKIIAPPERKYSVWIG 515
VLSGG+T++ +R+Q+++ + + + +I+ ++ +VW G
Sbjct: 330 IVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFG 389
Query: 516 GSILAXLXTXQQMWISKQEYDESGPFIVHR 605
GS+LA +K +Y+E G I R
Sbjct: 390 GSLLAQTPEFGSYCHTKADYEEYGASIARR 419
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 66.5 bits (155), Expect = 3e-12
Identities = 30/115 (26%), Positives = 60/115 (52%)
Frame = +3
Query: 246 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 425
E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 426 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAXLXTXQQMWISKQEYDESGP 590
+++E+T++ P I + W G S + + ++++EY E GP
Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGP 708
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 43.6 bits (98), Expect = 3e-05
Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
Frame = +3
Query: 360 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 530
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 531 XLXTXQQMWISKQEYDESGPFIVHRK 608
+ ++WI ++ G ++ K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612
>SPCC1682.16 |rpt4||19S proteasome regulatory subunit
Rpt4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 388
Score = 29.1 bits (62), Expect = 0.64
Identities = 21/63 (33%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = +2
Query: 77 RFSFTTTAEREIVRDIKEKLCYV-ALDFEQEMATAASSSFPREVLRTSRRSGHHYRKRKI 253
RFS T+A+REI R + E L + D+ + +++ P + R G RK +I
Sbjct: 241 RFSEGTSADREIQRTLMELLNQMDGFDYLGQTKIIMATNRPDTLDPALLRPGRLDRKIEI 300
Query: 254 PLP 262
PLP
Sbjct: 301 PLP 303
>SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 28.7 bits (61), Expect = 0.85
Identities = 11/35 (31%), Positives = 24/35 (68%)
Frame = +1
Query: 154 LRAGNGHRCIQQLPSRSLTNFPTVRSSLSETKDSV 258
L A + +C+Q LP + +FP+VR+ +++ ++S+
Sbjct: 506 LMATSLKKCVQSLPEKYAHSFPSVRNYIAKMENSM 540
>SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit
Sfc6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 582
Score = 27.1 bits (57), Expect = 2.6
Identities = 17/47 (36%), Positives = 22/47 (46%), Gaps = 2/47 (4%)
Frame = +1
Query: 181 IQQLPSRSLTNFPTVRSSL--SETKDSVAQRLSSNPRSWVWKLAAST 315
+ LP+ T F V L SET +SV R S + +WKL T
Sbjct: 212 VSWLPTNKETQFLAVGGMLKFSETTESVFMRTSGRNQIQLWKLENKT 258
>SPCC550.09 |||peroxin Pex32 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 535
Score = 26.2 bits (55), Expect = 4.5
Identities = 16/49 (32%), Positives = 23/49 (46%)
Frame = +3
Query: 75 RGSRSLPPPSGKSFVTSRRSCATSLLTSSRKWPPLHPAASLEKSYELPD 221
R R PP + TSR + A+SL ++S H + S K +PD
Sbjct: 480 RRYRVAPPAENEKPHTSRTNTASSLSSTSEDQVSTHGSISSAKVTAIPD 528
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,727,956
Number of Sequences: 5004
Number of extensions: 56591
Number of successful extensions: 230
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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