Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-2067
(720 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 292 4e-80
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 160 2e-40
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 156 3e-39
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 155 6e-39
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.7
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.6
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.7
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 4.7
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 6.2
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 25 8.2
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 8.2
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 292 bits (716), Expect = 4e-80
Identities = 131/187 (70%), Positives = 150/187 (80%)
Frame = +1
Query: 55 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 234
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 235 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 414
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 415 RKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVV 594
R+EAE+CD LQGFQ KIREEYPDR+M T+SV P+PK SDTVV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 595 EPYNATL 615
EPYNATL
Sbjct: 181 EPYNATL 187
Score = 49.6 bits (113), Expect = 4e-07
Identities = 21/34 (61%), Positives = 27/34 (79%)
Frame = +2
Query: 617 SVHQLXENTDETYCIDNEALYDICFRTLKLSTPT 718
S+HQL EN+DET+CIDNEAL I TLK+ +P+
Sbjct: 188 SMHQLVENSDETFCIDNEALSSIFANTLKIKSPS 221
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 160 bits (389), Expect = 2e-40
Identities = 78/189 (41%), Positives = 109/189 (57%), Gaps = 2/189 (1%)
Frame = +1
Query: 55 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 228
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 229 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 408
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 409 VVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDT 588
+R+ A++C LQGF ++ EY + +SV P+P+VS +
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 589 VVEPYNATL 615
VVEPYN+ L
Sbjct: 181 VVEPYNSVL 189
Score = 33.9 bits (74), Expect = 0.023
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +2
Query: 617 SVHQLXENTDETYCIDNEALYDICFRTLKLSTPT 718
+ H + D T+ +DNE+ YDIC R L + P+
Sbjct: 190 TTHATLDLADCTFMVDNESCYDICRRNLDIERPS 223
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 156 bits (378), Expect = 3e-39
Identities = 71/189 (37%), Positives = 116/189 (61%), Gaps = 3/189 (1%)
Frame = +1
Query: 58 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 237
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 238 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 411
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 412 VRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDT 588
+ +EA+ D L+GF ++ + YP +I+ TYSV P S VSD
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 589 VVEPYNATL 615
VV+PYN+ L
Sbjct: 182 VVQPYNSLL 190
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 155 bits (376), Expect = 6e-39
Identities = 75/193 (38%), Positives = 110/193 (56%), Gaps = 6/193 (3%)
Frame = +1
Query: 55 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 216
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 217 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 396
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 397 SVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 576
SVL+ +R+ A++C LQGF ++ EY + +SV P+P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Query: 577 VSDTVVEPYNATL 615
VS +VVEPYN+ L
Sbjct: 181 VSTSVVEPYNSVL 193
Score = 40.7 bits (91), Expect = 2e-04
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +2
Query: 617 SVHQLXENTDETYCIDNEALYDICFRTLKLSTPT 718
+ H +N+D T+ +DNEA YDIC R L + PT
Sbjct: 194 TTHATLDNSDCTFMVDNEACYDICRRNLDIERPT 227
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.7
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 94 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 195
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.6
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 289 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 390
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 4.7
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 358 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 447
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 4.7
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -2
Query: 431 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 294
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 6.2
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 310 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 441
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
Gap1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 766
Score = 25.4 bits (53), Expect = 8.2
Identities = 15/46 (32%), Positives = 27/46 (58%)
Frame = -1
Query: 207 VVYIDALQLQVRVPMVSTGGVDAVLVGDDLPELSSDLVAALTSLDM 70
++Y+DA L +++ + G A V DLP L +D V++L S+ +
Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 8.2
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 347 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 252
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,669,283
Number of Sequences: 5004
Number of extensions: 50739
Number of successful extensions: 166
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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