BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1967
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex al... 89 8e-19
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 27 2.9
SPCC320.08 |||membrane transporter |Schizosaccharomyces pombe|ch... 27 2.9
SPBC1348.10c |||phospholipase |Schizosaccharomyces pombe|chr 2||... 27 3.8
SPAC977.09c |||phospholipase |Schizosaccharomyces pombe|chr 1|||... 27 3.8
SPBC651.03c |gyp10||GTPase activating protein Gyp10|Schizosaccha... 25 8.7
>SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex alpha
subunit Egd2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 173
Score = 88.6 bits (210), Expect = 8e-19
Identities = 58/143 (40%), Positives = 78/143 (54%), Gaps = 7/143 (4%)
Frame = +2
Query: 308 KKARKIMSKLGLKPVQGVERVTIRKSKNILFVINSPDVYKNPHSDTYIVFGEAKIEDLST 487
+KA+K++ KLGLK V+G+ RV +R+ KNIL +IN P VYK+ ++ YIV G+ +ED++
Sbjct: 25 EKAQKLIQKLGLKRVEGITRVAMRRPKNILLIINEPIVYKS-SNNAYIVLGKVTVEDMAA 83
Query: 488 QATMAAAERFKAPET------TATGNDASTTGTTVAPIAE-XXXXXXXXXXXXXXXXXXX 646
QA + +A ET T DA T A I E
Sbjct: 84 QARAFNESQKQATETKEEAAITEESGDAQPADT--AKIEESFEQEKAVDETGVDAKDIEL 141
Query: 647 XMSQANVSRAKAVRALKNNQSDI 715
M+QANVSRAKAV ALK N SD+
Sbjct: 142 VMAQANVSRAKAVTALKENNSDV 164
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 27.1 bits (57), Expect = 2.9
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 95 QQYIKMPELTELDKATASMTEKRKEE 172
++YIK +LTELD +T+ RK +
Sbjct: 205 KEYIKKCQLTELDPTQQLLTQSRKNQ 230
>SPCC320.08 |||membrane transporter |Schizosaccharomyces pombe|chr
3|||Manual
Length = 505
Score = 27.1 bits (57), Expect = 2.9
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -3
Query: 376 DCHSLYTLYWFETEFTHYLSGLLL 305
D H LYTL+W +E YL+G +L
Sbjct: 347 DRHHLYTLHW-TSEMGGYLAGFIL 369
>SPBC1348.10c |||phospholipase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 673
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2
Query: 497 MAAAERFKAPETTATGNDASTTGTTVA 577
M+AA +AP T +G +STT ++VA
Sbjct: 627 MSAATTSRAPSGTTSGTASSTTSSSVA 653
>SPAC977.09c |||phospholipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 673
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2
Query: 497 MAAAERFKAPETTATGNDASTTGTTVA 577
M+AA +AP T +G +STT ++VA
Sbjct: 627 MSAATTSRAPSGTTSGTASSTTSSSVA 653
>SPBC651.03c |gyp10||GTPase activating protein
Gyp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 373
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = -3
Query: 379 SDCHSLYTLYWFETEFTHYLSGLLLTTRLFRF 284
+D + L W T F H +S + + RLF F
Sbjct: 184 ADIQCYFALSWLITWFAHDVSDISVVCRLFDF 215
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,558,149
Number of Sequences: 5004
Number of extensions: 45994
Number of successful extensions: 143
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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