Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1966
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 357 e-100
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 357 1e-99
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 357 1e-99
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 126 4e-30
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 122 5e-29
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 88 2e-18
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 33 0.062
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 1.3
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 3.1
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.1
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 27 4.1
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 26 5.4
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 5.4
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 9.5
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 25 9.5
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 357 bits (879), Expect = e-100
Identities = 168/240 (70%), Positives = 194/240 (80%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFE 180
ADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFE 166
Query: 181 EIKKEVSSYIKKIGYNPXAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 360
EI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTL 226
Query: 361 IEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTE 540
+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTE
Sbjct: 227 LEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTE 286
Query: 541 VKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRRGYVAGDSKNNPPXGAADFTAQVIGLN 720
VKSVEMHHE+L +PGD ++RRG V GDSKN+PP G A FTAQVI LN
Sbjct: 287 VKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILN 346
Score = 42.3 bits (95), Expect = 8e-05
Identities = 18/27 (66%), Positives = 19/27 (70%)
Frame = +3
Query: 720 HXGQISNGYXPVLDWHTGHIGXKFAXI 800
H GQIS GY PVLD HT HI KFA +
Sbjct: 347 HPGQISAGYSPVLDCHTAHIACKFAEL 373
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 357 bits (878), Expect = 1e-99
Identities = 167/240 (69%), Positives = 194/240 (80%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFE 180
ADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFE 166
Query: 181 EIKKEVSSYIKKIGYNPXAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 360
EI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTL 226
Query: 361 IEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTE 540
+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTE
Sbjct: 227 LEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTE 286
Query: 541 VKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRRGYVAGDSKNNPPXGAADFTAQVIGLN 720
VKSVEMHHE+L +PGD ++RRG V GDSKN+PP G A FTAQVI LN
Sbjct: 287 VKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILN 346
Score = 42.3 bits (95), Expect = 8e-05
Identities = 18/27 (66%), Positives = 19/27 (70%)
Frame = +3
Query: 720 HXGQISNGYXPVLDWHTGHIGXKFAXI 800
H GQIS GY PVLD HT HI KFA +
Sbjct: 347 HPGQISAGYSPVLDCHTAHIACKFAEL 373
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 357 bits (878), Expect = 1e-99
Identities = 167/240 (69%), Positives = 194/240 (80%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFE 180
ADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFE
Sbjct: 109 ADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFE 166
Query: 181 EIKKEVSSYIKKIGYNPXAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCL 360
EI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L
Sbjct: 167 EIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTL 226
Query: 361 IEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTE 540
+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTE
Sbjct: 227 LEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTE 286
Query: 541 VKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRRGYVAGDSKNNPPXGAADFTAQVIGLN 720
VKSVEMHHE+L +PGD ++RRG V GDSKN+PP G A FTAQVI LN
Sbjct: 287 VKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILN 346
Score = 42.3 bits (95), Expect = 8e-05
Identities = 18/27 (66%), Positives = 19/27 (70%)
Frame = +3
Query: 720 HXGQISNGYXPVLDWHTGHIGXKFAXI 800
H GQIS GY PVLD HT HI KFA +
Sbjct: 347 HPGQISAGYSPVLDCHTAHIACKFAEL 373
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 126 bits (304), Expect = 4e-30
Identities = 78/241 (32%), Positives = 129/241 (53%), Gaps = 5/241 (2%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFE 180
AD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD +SE R++
Sbjct: 340 ADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYK 399
Query: 181 EIKKEVSSYIKKI-GYNPXA-VAFVPISGWHGDNMLE--PSTKMPWFKGWQVERKEGKAD 348
E ++S +++++ GYN V ++P+S + G N+ + S+ PW++
Sbjct: 400 ECVDKLSMFLRRVAGYNSKTDVKYMPVSAYTGQNVKDRVDSSVCPWYQ------------ 447
Query: 349 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 528
G L+E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N
Sbjct: 448 GPSLLEYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPIN 505
Query: 529 ITTEVKSV-EMHHEALQEAVPGDXXXXXXXXXXXXELRRGYVAGDSKNNPPXGAADFTAQ 705
T EV ++ + E + ++ GD +++ GYV +K NP F AQ
Sbjct: 506 QTLEVTAIYDEADEEISSSICGD-QVRLRVRGDDSDVQTGYVLTSTK-NPVHATTRFIAQ 563
Query: 706 V 708
+
Sbjct: 564 I 564
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 122 bits (295), Expect = 5e-29
Identities = 85/240 (35%), Positives = 122/240 (50%), Gaps = 4/240 (1%)
Frame = +1
Query: 1 ADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFE 180
AD AVL+V + FE G +NGQTREHA L LG+ +++V VNK+D +SE RF+
Sbjct: 279 ADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQ 336
Query: 181 EIKKEVSSY-IKKIGYNPXAVAFVPISGWHGDNML--EPSTKMPWFKGWQVERKEGKADG 351
EIK VS + IK +G+ V FVPIS G N++ + S W+K G
Sbjct: 337 EIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYK------------G 384
Query: 352 KCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANI 531
L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++ +
Sbjct: 385 PTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQE 443
Query: 532 TTEVKSVEMHHEALQE-AVPGDXXXXXXXXXXXXELRRGYVAGDSKNNPPXGAADFTAQV 708
VK+V + + AV GD +LR G + + + NP F A++
Sbjct: 444 DAYVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPGDILSNYE-NPVRRVRSFVAEI 502
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 87.8 bits (208), Expect = 2e-18
Identities = 71/220 (32%), Positives = 108/220 (49%), Gaps = 4/220 (1%)
Frame = +1
Query: 4 DCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFEE 183
D A+++V+A G+ QTREH LLA +GVKQ++V +NK+D EP E E
Sbjct: 141 DGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEME 193
Query: 184 IKKEVSSYIKKIGYNPXAVAFVPISGWHGDNM-LEPSTKMPWFKGWQVERKEGKADGKCL 360
+++ +S Y G+ GDN + + + +G + E G L
Sbjct: 194 MRELLSEY-----------------GFDGDNTPIVSGSALCALEGREPEI--GLNSITKL 234
Query: 361 IEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPANI 531
+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G +V +++
Sbjct: 235 MEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHL 294
Query: 532 TTEVKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRRGYV 651
T V +EM + L AV GD +L+RG +
Sbjct: 295 KTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 32.7 bits (71), Expect = 0.062
Identities = 26/94 (27%), Positives = 40/94 (42%)
Frame = +1
Query: 298 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 477
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 478 ETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE 579
G +K G V+ EV+ V + + + E
Sbjct: 266 MEGFIKKGGKVMSVNTGRKYEVQQVGIMYPDMTE 299
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -1
Query: 398 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 261
G GG S+ P+ LPS + P L E +ML CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 3.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -1
Query: 542 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 426
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.1
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 636 QFLDGHVLYVETYIVSRYSFLESFV 562
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 26.6 bits (56), Expect = 4.1
Identities = 19/74 (25%), Positives = 36/74 (48%)
Frame = -3
Query: 549 RLDFSSDVGGGKDNNGTWFQHTSFNSADGHGTNTTDFVYVLQGKTQGLVSGAGRWQDGIE 370
+L+F+ VG D+ + + + + DF+ ++GK L + R+++ E
Sbjct: 120 KLEFT--VGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNE 177
Query: 369 SFNEAFSVSFAFLT 328
S NE +FA+LT
Sbjct: 178 STNERVK-NFAYLT 190
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 26.2 bits (55), Expect = 5.4
Identities = 12/47 (25%), Positives = 22/47 (46%)
Frame = +1
Query: 70 QTREHALLAFTLGVKQLIVXVNKMDSTEPPYSEPRFEEIKKEVSSYI 210
QT EH + +K +I+ NK+D +E ++ I K + +
Sbjct: 149 QTSEHLAAIEIMQLKHIIILQNKVDLIRESAAEEHYQSILKFIKGTV 195
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 5.4
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = -1
Query: 500 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 336
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 335 SLRSTCHPLNQG 300
P G
Sbjct: 374 PADGVPKPTPDG 385
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 491 NTPVSTLPTGTVPIPPIL 438
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 25.4 bits (53), Expect = 9.5
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = +1
Query: 139 MDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPXAVAFVPISGWHGDNMLEPSTKMPWF 309
+D +P + F IKK+V Y+ ++ + P + G N LEP TK PW+
Sbjct: 107 LDLKKPLLPQILFGNIKKDV--YLDQV-HRPRHYRGSGSAPLFG-NFLEPLTKTPWY 159
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.315 0.134 0.400
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,300,815
Number of Sequences: 5004
Number of extensions: 68657
Number of successful extensions: 171
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
- SilkBase 1999-2023 -