Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1954
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC29A10.05 |exo1|mut2|exonuclease I Exo1|Schizosaccharomyces p... 174 1e-44
SPAC3G6.06c |rad2|fen1|FEN-1 endonuclease|Schizosaccharomyces po... 52 1e-07
SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyce... 44 3e-05
SPAC139.01c ||SPAC955.02c|nuclease, XP-G family|Schizosaccharomy... 36 0.007
SPAC12G12.16c ||SPAC18B11.01c|nuclease, XP-G family|Schizosaccha... 31 0.25
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 30 0.44
SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 28 1.3
SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 27 2.3
SPCC1442.07c |||ubiquitin/metalloprotease fusion protein|Schizos... 26 7.2
SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|c... 25 9.5
>SPBC29A10.05 |exo1|mut2|exonuclease I Exo1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 571
Score = 174 bits (423), Expect = 1e-44
Identities = 87/190 (45%), Positives = 116/190 (61%)
Frame = +3
Query: 33 MGITGLIPFIEKASRRTDVSEFSGCTVAIDSYCWLHKGAFACADKLVRGEETDMHIKYCL 212
MGI GL+ ++ + + V EFSG T+ +D Y WLHK F CA +L +ETD ++KY +
Sbjct: 1 MGIKGLLGLLKPMQKSSHVEEFSGKTLGVDGYVWLHKAVFTCAHELAFNKETDKYLKYAI 60
Query: 213 KYMTMLLSKNIKPILVFDGRHLPAKAMTESKRRESRNISKKRAAELLSLGKIEEARSYLR 392
ML +KP++VFDG LP KA TE KR+E R + + +L GK +A
Sbjct: 61 HQALMLQYYGVKPLIVFDGGPLPCKASTEQKRKERRQEAFELGKKLWDEGKKSQAIMQFS 120
Query: 393 RSVDITHAMALDLIXECRKMNVDCIVAPYEADAQLAYLNIKNIAQLVITEDSDLILFGCT 572
R VD+T MA LI R+ ++ IVAPYEADAQL YL +NI +ITEDSD+++FG
Sbjct: 121 RCVDVTPEMAWKLIIALREHGIESIVAPYEADAQLVYLEKENIIDGIITEDSDMLVFGAQ 180
Query: 573 XVLFKMDLHG 602
VLFKMD G
Sbjct: 181 TVLFKMDGFG 190
>SPAC3G6.06c |rad2|fen1|FEN-1 endonuclease|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 380
Score = 52.0 bits (119), Expect = 1e-07
Identities = 47/192 (24%), Positives = 80/192 (41%), Gaps = 9/192 (4%)
Frame = +3
Query: 33 MGITGLIPFIEKAS----RRTDVSEFSGCTVAIDS----YCWLHKGAFACADKLVRGE-E 185
MGI GL + + + + D+ + G VAID+ Y +L + +L+ + E
Sbjct: 1 MGIKGLAQVLSEHAPASVKHNDIKNYFGRKVAIDASMSLYQFLIQVRSQDGQQLMNEQGE 60
Query: 186 TDMHIKYCLKYMTMLLSKNIKPILVFDGRHLPAKAMTESKRRESRNISKKRAAELLSLGK 365
T H+ ++ IKP VFDG+ K+ +KR +++ E +G
Sbjct: 61 TTSHLMGMFYRTLRIVDNGIKPCFVFDGKPPTLKSGELAKRVARHQKAREDQEETKEVGT 120
Query: 366 IEEARSYLRRSVDITHAMALDLIXECRKMNVDCIVAPYEADAQLAYLNIKNIAQLVITED 545
E + +R+V +T + M + + AP EA+AQ A L +ED
Sbjct: 121 AEMVDRFAKRTVKVTRQHNDEAKRLLELMGIPFVNAPCEAEAQCAALARSGKVYAAASED 180
Query: 546 SDLILFGCTXVL 581
D + F +L
Sbjct: 181 MDTLCFQAPVLL 192
>SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1112
Score = 43.6 bits (98), Expect = 3e-05
Identities = 28/96 (29%), Positives = 43/96 (44%)
Frame = +3
Query: 33 MGITGLIPFIEKASRRTDVSEFSGCTVAIDSYCWLHKGAFACADKLVRGEETDMHIKYCL 212
MG++GL +E R + +AID+ W+++ A DK + H+
Sbjct: 1 MGVSGLWDILEPVKRPVKLETLVNKRLAIDASIWIYQFLKAVRDK-EGNQLKSSHVVGFF 59
Query: 213 KYMTMLLSKNIKPILVFDGRHLPAKAMTESKRRESR 320
+ + LL IKP+ VFDG K T KR+ R
Sbjct: 60 RRICKLLFFGIKPVFVFDGGAPSLKRQTIQKRQARR 95
Score = 42.3 bits (95), Expect = 8e-05
Identities = 25/73 (34%), Positives = 38/73 (52%)
Frame = +3
Query: 372 EARSYLRRSVDITHAMALDLIXECRKMNVDCIVAPYEADAQLAYLNIKNIAQLVITEDSD 551
+ RS R + ++T M + R + IVAP EA+AQ + L + ++T+DSD
Sbjct: 741 QKRSEKRDADEVTQVMIKECQELLRLFGLPYIVAPQEAEAQCSKLLELKLVDGIVTDDSD 800
Query: 552 LILFGCTXVLFKM 590
+ LFG T V M
Sbjct: 801 VFLFGGTRVYRNM 813
>SPAC139.01c ||SPAC955.02c|nuclease, XP-G family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 802
Score = 35.9 bits (79), Expect = 0.007
Identities = 56/230 (24%), Positives = 91/230 (39%), Gaps = 29/230 (12%)
Frame = +3
Query: 33 MGITGLIPFI--EKASRRTDVSEFSGCTVAIDSYCWL----HKGAFACADKLVRGEETDM 194
M I L FI +K ++ +S F C + ID+ +L H L E++
Sbjct: 1 MTIRSLNLFIIDKKHQHKSSLSSFQNCKLGIDASFYLTQIIHSFTPQELQSLAVNGESEY 60
Query: 195 HIKYCLKYMTMLLSKNIKPILVFDGRHL----------PAKAMTESKRRE---------- 314
+++ L ++NI PI VF+G L P K + S +
Sbjct: 61 LQHRISEFLEQLRTENITPIFVFNGIPLTFEASSQLEVPGKQKSHSALTDFEAFDPYDAN 120
Query: 315 -SRNISKKRAAELLSLGKIEEARSYLRRSVDITHAMALDLIXECRKMNVDCIVAPYEADA 491
RN+ + A+ + G+ + Y + D + + + NV+ VAPY A A
Sbjct: 121 IQRNMYRMDASGPANYGESKPTLLYTNQR-DHLDRLCDQVKFYLDQCNVEYFVAPYLAMA 179
Query: 492 QLAY-LNIKNIAQL-VITEDSDLILFGCTXVLFKMDLHGTXTLVEHPKLP 635
QLAY LN + + I +DL+LFG + M+ + P P
Sbjct: 180 QLAYFLNGTSSPYIDAIYGSTDLLLFGVKKFITSMNTSSNVKISSDPSSP 229
>SPAC12G12.16c ||SPAC18B11.01c|nuclease, XP-G
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 496
Score = 30.7 bits (66), Expect = 0.25
Identities = 19/69 (27%), Positives = 37/69 (53%)
Frame = +3
Query: 480 EADAQLAYLNIKNIAQLVITEDSDLILFGCTXVLFKMDLHGTXTLVEHPKLPHVMKCPIQ 659
EA+A + ++ N+A V T+D+D++L G + + +DL+ L P + +
Sbjct: 300 EAEAFASAISQNNLAYAVATQDTDVLLLGSSMISNFLDLNDNFHLPLQIMDPRKIAQEL- 358
Query: 660 HYTFDKFRE 686
+ TFD F++
Sbjct: 359 NLTFDGFQD 367
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 29.9 bits (64), Expect = 0.44
Identities = 14/68 (20%), Positives = 36/68 (52%)
Frame = +3
Query: 252 ILVFDGRHLPAKAMTESKRRESRNISKKRAAELLSLGKIEEARSYLRRSVDITHAMALDL 431
I+ F+ + ++ + ++S K++++ ++ +EE SYL++ +D +A DL
Sbjct: 519 IIEFEDQLARLSSVRNNSIKQSTTFQVKKSSQKSTIQNLEEKVSYLQQFMDKNNATLTDL 578
Query: 432 IXECRKMN 455
+C ++
Sbjct: 579 EFQCSDLS 586
>SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 2386
Score = 28.3 bits (60), Expect = 1.3
Identities = 19/62 (30%), Positives = 33/62 (53%)
Frame = +3
Query: 345 ELLSLGKIEEARSYLRRSVDITHAMALDLIXECRKMNVDCIVAPYEADAQLAYLNIKNIA 524
EL K++E L ++DI++ + LD + C +DC V + Q++YL KN+
Sbjct: 1051 ELFFQAKVDELHDTL--NLDISNEV-LDQLLRCL---LDCCVKYASTNMQISYLAAKNLG 1104
Query: 525 QL 530
+L
Sbjct: 1105 EL 1106
>SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit
Apc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1458
Score = 27.5 bits (58), Expect = 2.3
Identities = 11/29 (37%), Positives = 19/29 (65%)
Frame = -2
Query: 382 ERASSIFPKLKSSAALFFEIFRDSRLFDS 296
E ++FPKLK +++ + + + SRLF S
Sbjct: 1337 EEPLNLFPKLKDTSSPLWNLVKTSRLFQS 1365
>SPCC1442.07c |||ubiquitin/metalloprotease fusion
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 282
Score = 25.8 bits (54), Expect = 7.2
Identities = 16/58 (27%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +3
Query: 510 IKNIAQLV--ITEDSDLILFGCTXVLFKMDLHGTXTLVEHPKLPHVMKCPIQHYTFDK 677
+KN ++++ I +D D++ + + K+ + T T PK PH P YTF++
Sbjct: 58 VKNESKIMCLIRQDKDIVNQAISQL--KVPDYSTNTYSLKPKKPHTTPKPASIYTFNE 113
>SPAC24H6.11c |||sulfate transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 958
Score = 25.4 bits (53), Expect = 9.5
Identities = 14/42 (33%), Positives = 24/42 (57%)
Frame = +2
Query: 239 EHKTNLSI*WTTSPS*SNDRIKKARISKYFKEKSSRAFKFRK 364
+HK N+S+ T SPS +D +++ +S K ++ KF K
Sbjct: 763 KHKQNISMAETFSPSPRHDLLRQVAMSS-VKAETKELSKFEK 803
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,059,235
Number of Sequences: 5004
Number of extensions: 59406
Number of successful extensions: 165
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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