BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1942
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC106.11c |plg7||phospholipase A2 |Schizosaccharomyces pombe|c... 27 2.3
SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon... 27 3.1
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 27 4.1
SPBC83.10 |||conserved eukaryotic protein|Schizosaccharomyces po... 26 5.4
SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein Ndc80|... 25 9.5
SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 9.5
SPAC6B12.15 |cpc2|rkp1|RACK1 homologue Cpc2|Schizosaccharomyces ... 25 9.5
SPBC24C6.05 |sec28||coatomer epsilon subunit |Schizosaccharomyce... 25 9.5
>SPBC106.11c |plg7||phospholipase A2 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 438
Score = 27.5 bits (58), Expect = 2.3
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 115 MSHSNVKSSVMYPYYQLPVDKLHV 186
M H+ K S++Y + LPV +LH+
Sbjct: 279 MFHTEFKCSILYDIWMLPVRQLHL 302
>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 678
Score = 27.1 bits (57), Expect = 3.1
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -3
Query: 621 NVVRDGVIRERATLHQTE*RTELPVFVF*K 532
N + V+RE + H+T RTE VFV K
Sbjct: 152 NAIMTMVVREASPFHRTRARTESSVFVIDK 181
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 26.6 bits (56), Expect = 4.1
Identities = 33/135 (24%), Positives = 58/135 (42%), Gaps = 7/135 (5%)
Frame = +1
Query: 202 MQELYLNVKGSEESEGTERTVGSSQAPRFTKTDS-EEFDDAP-----DLCMTNYDTLQVI 363
+++L L G +S + +VG + P F+ T S + F +L + NYDT I
Sbjct: 59 LEKLKLRKNGVRKSNSEKPSVGIEKNPSFSTTKSAKSFSSTSSNIDSNLDLLNYDTPLTI 118
Query: 364 HGKIYVFEEEWVTVLRDRNVREPGYPKRFHELFIGLPSSIKVIRTIYEK-HDRNVVVFSE 540
++ ++ L + E Y K I+ + +YE+ HDR + +E
Sbjct: 119 SKISFLLQQ-----LEFKLSVEEQYRK-----------GIEKMAKLYEREHDRRSIAEAE 162
Query: 541 NKYWEFSPSFRLMKR 585
KY E + L+K+
Sbjct: 163 KKYVESAQKITLLKQ 177
>SPBC83.10 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 189
Score = 26.2 bits (55), Expect = 5.4
Identities = 9/34 (26%), Positives = 17/34 (50%)
Frame = +1
Query: 76 FAVAVHXIGHSLGMSHSNVKSSVMYPYYQLPVDK 177
+ + + I + H + S++YPYY P +K
Sbjct: 77 YFLRLESIDYEFSEFHIIINESIVYPYYTSPAEK 110
>SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein
Ndc80|Schizosaccharomyces pombe|chr 2|||Manual
Length = 624
Score = 25.4 bits (53), Expect = 9.5
Identities = 18/82 (21%), Positives = 32/82 (39%)
Frame = +1
Query: 169 VDKLHVDDILGMQELYLNVKGSEESEGTERTVGSSQAPRFTKTDSEEFDDAPDLCMTNYD 348
VDK+ D ++ LYL+ E E +Q + +E + +
Sbjct: 230 VDKVLFDYLVRTYHLYLDESPEESEPEKELKATFNQQNQDLYNQTEALKSTNEELINQIK 289
Query: 349 TLQVIHGKIYVFEEEWVTVLRD 414
+ + + I V EE + T+ RD
Sbjct: 290 SAEELDSAIQVLEERYRTMQRD 311
>SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 452
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/23 (43%), Positives = 18/23 (78%)
Frame = +2
Query: 458 FSLVSRPASKLYGPSMRNTIGTL 526
FSLVS + +YGPS ++++G++
Sbjct: 68 FSLVSPGWASIYGPSGQSSVGSV 90
>SPAC6B12.15 |cpc2|rkp1|RACK1 homologue Cpc2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 314
Score = 25.4 bits (53), Expect = 9.5
Identities = 14/46 (30%), Positives = 24/46 (52%)
Frame = +1
Query: 508 KHDRNVVVFSENKYWEFSPSFRLMKRGSLTDYSIPDNVTELTTVFI 645
K + N +VFS N+YW + + ++ + D + V ELT F+
Sbjct: 233 KANINALVFSPNRYWLCAATGSSIR---IFDLETQEKVDELTVDFV 275
>SPBC24C6.05 |sec28||coatomer epsilon subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 288
Score = 25.4 bits (53), Expect = 9.5
Identities = 25/115 (21%), Positives = 52/115 (45%), Gaps = 1/115 (0%)
Frame = +1
Query: 199 GMQELYLNVKGSEESEGTERTVGSSQAPRFTKTDSEEFDDAPDLCMTNYDTLQVIHGKIY 378
G EL ++ G+ +S +T+G+ + + FDDA DL + + L+ + ++Y
Sbjct: 82 GNMELIIDQHGNSDS--VVQTLGA-----IFQIKNGSFDDAMDLLKKSVENLEAVALQVY 134
Query: 379 VFEEEWVTVLRDRNVREPGYPKRFHELFIGLPSS-IKVIRTIYEKHDRNVVVFSE 540
+ E ++ +++ E+ + L S IK++ E ++ VF E
Sbjct: 135 IHLREHKIEAAEQTLKQ-ALDWADEEIVLQLAQSWIKIVSGGVESYNDAFYVFEE 188
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,045,632
Number of Sequences: 5004
Number of extensions: 61348
Number of successful extensions: 176
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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