Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1872
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 328 7e-91
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 328 7e-91
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 328 7e-91
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 117 2e-27
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 113 2e-26
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 87 4e-18
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 33 0.062
SPAC3A11.02 |cps3|mug188|zinc finger protein Cps3|Schizosaccharo... 28 1.3
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 1.3
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 27 3.1
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 3.1
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 27 4.1
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 5.4
SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit ... 26 7.2
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 9.5
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 25 9.5
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 328 bits (805), Expect = 7e-91
Identities = 155/227 (68%), Positives = 179/227 (78%)
Frame = +1
Query: 1 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 180
LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE
Sbjct: 114 LIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKE 171
Query: 181 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 360
S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D
Sbjct: 172 TSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAID 231
Query: 361 AILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVE 540
+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVE
Sbjct: 232 SIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVE 291
Query: 541 MHHEALQEAVPGDNVGFNVKERVRQGIASWVCCW*LPKQPT*GCCRF 681
MHHE+L +PGDNVGFNVK + I C P GC F
Sbjct: 292 MHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF 338
Score = 61.3 bits (142), Expect = 2e-10
Identities = 27/37 (72%), Positives = 31/37 (83%)
Frame = +2
Query: 599 KNVSVKELRRGYVAGDSQNNPPKGAADFTAQVIXLNH 709
KNVSVK++RRG V GDS+N+PP G A FTAQVI LNH
Sbjct: 311 KNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNH 347
Score = 44.8 bits (101), Expect = 1e-05
Identities = 19/31 (61%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
Frame = +3
Query: 711 PGQISNGYXPVLDCHTGHIGCNL-QKXXKVD 800
PGQIS GY PVLDCHT HI C + K+D
Sbjct: 348 PGQISAGYSPVLDCHTAHIACKFAELIEKID 378
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 328 bits (805), Expect = 7e-91
Identities = 155/227 (68%), Positives = 179/227 (78%)
Frame = +1
Query: 1 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 180
LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE
Sbjct: 114 LIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKE 171
Query: 181 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 360
S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D
Sbjct: 172 TSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAID 231
Query: 361 AILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVE 540
+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVE
Sbjct: 232 SIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVE 291
Query: 541 MHHEALQEAVPGDNVGFNVKERVRQGIASWVCCW*LPKQPT*GCCRF 681
MHHE+L +PGDNVGFNVK + I C P GC F
Sbjct: 292 MHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF 338
Score = 61.3 bits (142), Expect = 2e-10
Identities = 27/37 (72%), Positives = 31/37 (83%)
Frame = +2
Query: 599 KNVSVKELRRGYVAGDSQNNPPKGAADFTAQVIXLNH 709
KNVSVK++RRG V GDS+N+PP G A FTAQVI LNH
Sbjct: 311 KNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNH 347
Score = 44.8 bits (101), Expect = 1e-05
Identities = 19/31 (61%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
Frame = +3
Query: 711 PGQISNGYXPVLDCHTGHIGCNL-QKXXKVD 800
PGQIS GY PVLDCHT HI C + K+D
Sbjct: 348 PGQISAGYSPVLDCHTAHIACKFAELIEKID 378
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 328 bits (805), Expect = 7e-91
Identities = 155/227 (68%), Positives = 179/227 (78%)
Frame = +1
Query: 1 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 180
LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE
Sbjct: 114 LIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQARFEEIVKE 171
Query: 181 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 360
S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D
Sbjct: 172 TSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAID 231
Query: 361 AILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVE 540
+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVE
Sbjct: 232 SIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVE 291
Query: 541 MHHEALQEAVPGDNVGFNVKERVRQGIASWVCCW*LPKQPT*GCCRF 681
MHHE+L +PGDNVGFNVK + I C P GC F
Sbjct: 292 MHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF 338
Score = 61.3 bits (142), Expect = 2e-10
Identities = 27/37 (72%), Positives = 31/37 (83%)
Frame = +2
Query: 599 KNVSVKELRRGYVAGDSQNNPPKGAADFTAQVIXLNH 709
KNVSVK++RRG V GDS+N+PP G A FTAQVI LNH
Sbjct: 311 KNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNH 347
Score = 44.8 bits (101), Expect = 1e-05
Identities = 19/31 (61%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
Frame = +3
Query: 711 PGQISNGYXPVLDCHTGHIGCNL-QKXXKVD 800
PGQIS GY PVLDCHT HI C + K+D
Sbjct: 348 PGQISAGYSPVLDCHTAHIACKFAELIEKID 378
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 117 bits (282), Expect = 2e-27
Identities = 68/205 (33%), Positives = 115/205 (56%), Gaps = 5/205 (2%)
Frame = +1
Query: 1 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 180
L+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD +SE R++E +
Sbjct: 345 LVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDK 404
Query: 181 VSSYIKKI-GYNPAA-VAFVPISGWHGDNMLE--PSTKMPWFKGWQVERKEGKADGKCLI 348
+S +++++ GYN V ++P+S + G N+ + S+ PW++ G L+
Sbjct: 405 LSMFLRRVAGYNSKTDVKYMPVSAYTGQNVKDRVDSSVCPWYQ------------GPSLL 452
Query: 349 EALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEV 528
E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N T EV
Sbjct: 453 EYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEV 510
Query: 529 KSV-EMHHEALQEAVPGDNVGFNVK 600
++ + E + ++ GD V V+
Sbjct: 511 TAIYDEADEEISSSICGDQVRLRVR 535
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 113 bits (273), Expect = 2e-26
Identities = 75/199 (37%), Positives = 107/199 (53%), Gaps = 4/199 (2%)
Frame = +1
Query: 1 LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 180
L+V + FE G +NGQTREHA L LG+ +++V VNK+D +SE RF+EIK
Sbjct: 284 LVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNI 341
Query: 181 VSSY-IKKIGYNPAAVAFVPISGWHGDNML--EPSTKMPWFKGWQVERKEGKADGKCLIE 351
VS + IK +G+ + V FVPIS G N++ + S W+K G L+
Sbjct: 342 VSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYK------------GPTLLS 389
Query: 352 ALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVK 531
ALD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++ + VK
Sbjct: 390 ALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVK 448
Query: 532 SVEMHHEALQE-AVPGDNV 585
+V + + AV GD V
Sbjct: 449 NVIRNSDPSSTWAVAGDTV 467
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 86.6 bits (205), Expect = 4e-18
Identities = 66/191 (34%), Positives = 98/191 (51%), Gaps = 4/191 (2%)
Frame = +1
Query: 55 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFV 234
QTREH LLA +GVKQ++V +NK+D EP E E+++ +S Y
Sbjct: 156 QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEMEMRELLSEY-------------- 201
Query: 235 PISGWHGDNM-LEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILP-PARPTDKPLRLP 408
G+ GDN + + + +G + E G L+EA+D+ + P R TD P +
Sbjct: 202 ---GFDGDNTPIVSGSALCALEGREPEI--GLNSITKLMEAVDSYITLPERKTDVPFLMA 256
Query: 409 LQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPANITTEVKSVEMHHEALQEAVPGDN 582
++DV+ I G GTV GRVE G LK G +V +++ T V +EM + L AV GDN
Sbjct: 257 IEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDN 316
Query: 583 VGFNVKERVRQ 615
G ++ R+
Sbjct: 317 CGLLLRSIKRE 327
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 32.7 bits (71), Expect = 0.062
Identities = 26/94 (27%), Positives = 40/94 (42%)
Frame = +1
Query: 283 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 462
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 463 ETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE 564
G +K G V+ EV+ V + + + E
Sbjct: 266 MEGFIKKGGKVMSVNTGRKYEVQQVGIMYPDMTE 299
>SPAC3A11.02 |cps3|mug188|zinc finger protein
Cps3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 583
Score = 28.3 bits (60), Expect = 1.3
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
Frame = +3
Query: 549 RSSPRSCTWRQCRFQRKRTCPS-RNCVVG--MLLVTPKT 656
R SP+S C+F R+ TC S +NC+ + L T KT
Sbjct: 30 RPSPKSLQHVPCKFFRQGTCTSGKNCIFSHDLELATEKT 68
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 28.3 bits (60), Expect = 1.3
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -1
Query: 383 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 246
G GG S+ P+ LPS + P L E +ML CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 27.1 bits (57), Expect = 3.1
Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = +2
Query: 146 TVSPDLRKSRRKYPHTSRRLATTQLL---SLSCPFLDGTETTCWSLQPKCLGSRDGRWSV 316
+ +PD+ ++PHT +R +T + L LSC + +E + P G +D + +
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRCLSCEYSTLSEASDSMSNPLFFGDQDNGYVI 204
Query: 317 RKA 325
++
Sbjct: 205 SQS 207
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 3.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -1
Query: 527 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 411
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 26.6 bits (56), Expect = 4.1
Identities = 19/74 (25%), Positives = 36/74 (48%)
Frame = -3
Query: 534 RLDFSSDVGGGKDNNGTWFQHTSFNSADGHGTNTTDFVYVLQGKTQGLVSGAGRWQDGIE 355
+L+F+ VG D+ + + + + DF+ ++GK L + R+++ E
Sbjct: 120 KLEFT--VGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNE 177
Query: 354 SFNEAFSVSFAFLT 313
S NE +FA+LT
Sbjct: 178 STNERVK-NFAYLT 190
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 5.4
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = -1
Query: 485 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 321
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 320 SLRSTCHPLNQG 285
P G
Sbjct: 374 PADGVPKPTPDG 385
>SPCC16C4.14c |sfc4||transcription factor TFIIIC complex subunit
Sfc4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1006
Score = 25.8 bits (54), Expect = 7.2
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +2
Query: 686 AQVIXLNHSWSNLXRL 733
A+++ L+H WSNL RL
Sbjct: 329 AELLLLDHQWSNLIRL 344
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 476 NTPVSTLPTGTVPIPPIL 423
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 25.4 bits (53), Expect = 9.5
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = +1
Query: 124 MDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 294
+D +P + F IKK+V Y+ ++ + P + G N LEP TK PW+
Sbjct: 107 LDLKKPLLPQILFGNIKKDV--YLDQV-HRPRHYRGSGSAPLFG-NFLEPLTKTPWY 159
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,547,115
Number of Sequences: 5004
Number of extensions: 78752
Number of successful extensions: 284
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 254
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 276
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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