BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1861
(500 letters)
Database: mosquito
2352 sequences; 563,979 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF513639-1|AAM53611.1| 195|Anopheles gambiae glutathione S-tran... 105 1e-24
L07880-1|AAA29358.1| 218|Anopheles gambiae glutathione S-transf... 101 1e-23
AF316635-1|AAG45163.1| 224|Anopheles gambiae glutathione S-tran... 36 0.001
AY063776-1|AAL59658.1| 224|Anopheles gambiae glutathione S-tran... 35 0.002
Z81291-1|CAB03592.1| 209|Anopheles gambiae GSTD1-5 protein prot... 31 0.017
AF071160-3|AAC79993.1| 209|Anopheles gambiae glutathione S-tran... 31 0.017
AF515521-1|AAM61888.1| 233|Anopheles gambiae glutathione S-tran... 31 0.029
AY255857-1|AAP13483.1| 216|Anopheles gambiae glutathione tranfe... 30 0.051
AF316636-1|AAG45164.1| 221|Anopheles gambiae glutathione S-tran... 30 0.051
AY070256-1|AAL59655.1| 227|Anopheles gambiae glutathione S-tran... 29 0.089
AF515522-1|AAM61889.1| 222|Anopheles gambiae glutathione S-tran... 29 0.089
Z71481-1|CAA96105.1| 140|Anopheles gambiae GSTD2 protein protein. 27 0.27
AF515526-1|AAM61893.1| 229|Anopheles gambiae glutathione S-tran... 27 0.36
AF071162-1|AAC79998.1| 216|Anopheles gambiae glutathione S-tran... 27 0.36
AF071160-2|AAC79994.1| 216|Anopheles gambiae glutathione S-tran... 27 0.36
Z81292-1|CAB03593.1| 209|Anopheles gambiae GSTD1-6 protein prot... 26 0.63
AF071160-1|AAC79995.1| 209|Anopheles gambiae glutathione S-tran... 26 0.63
AY255856-1|AAP13482.1| 248|Anopheles gambiae glutathione transf... 25 1.1
AY578799-1|AAT07304.1| 679|Anopheles gambiae brinker protein. 25 1.4
AY070255-1|AAL59654.1| 230|Anopheles gambiae glutathione S-tran... 25 1.4
AF203335-1|AAF19830.1| 175|Anopheles gambiae immune-responsive ... 24 2.5
AF515471-1|AAM61879.1| 225|Anopheles gambiae glutathione S-tran... 24 3.3
AF491816-1|AAM09542.2| 225|Anopheles gambiae glutathione S-tran... 24 3.3
AF316637-1|AAG45165.1| 224|Anopheles gambiae glutathione S-tran... 24 3.3
AF513637-1|AAM53609.1| 214|Anopheles gambiae glutathione S-tran... 23 4.4
>AF513639-1|AAM53611.1| 195|Anopheles gambiae glutathione
S-transferase S1-2 protein.
Length = 195
Score = 105 bits (251), Expect = 1e-24
Identities = 53/118 (44%), Positives = 66/118 (55%)
Frame = +2
Query: 140 RLLLAYGGQEFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYGLA 319
R LL+YG F+D RI+ E WP KP P GQMPVLE+DGK+ QS A+ RYL + GLA
Sbjct: 11 RFLLSYGNLPFDDVRITREEWPALKPTMPMGQMPVLEVDGKKVHQSVAMSRYLANQVGLA 70
Query: 320 GANDEEAFEIDQNVEFLNDIRASAASVHYXXXXXXXXXXXXXXXXXXYPFFFEKLNEI 493
GA+D E ID V+ +ND R A V Y PF+ EKL++I
Sbjct: 71 GADDWENLMIDTVVDTVNDFRLKIAVVSYEPDDEIKEKKLVTLNNEVIPFYLEKLDDI 128
>L07880-1|AAA29358.1| 218|Anopheles gambiae glutathione
S-transferase protein.
Length = 218
Score = 101 bits (242), Expect = 1e-23
Identities = 51/118 (43%), Positives = 64/118 (54%)
Frame = +2
Query: 140 RLLLAYGGQEFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYGLA 319
R LL+YG F+D RI+ E WP KP P QMPVLE+DGK+ QS A+CRY+ ++ LA
Sbjct: 34 RFLLSYGNLPFDDVRITREEWPALKPTMPMRQMPVLEVDGKRVHQSLAMCRYVAKQINLA 93
Query: 320 GANDEEAFEIDQNVEFLNDIRASAASVHYXXXXXXXXXXXXXXXXXXYPFFFEKLNEI 493
G N EA +ID V+ +ND R A V Y PF+ KLN I
Sbjct: 94 GDNPLEALQIDAIVDTINDFRLKIAIVAYEPDDMVKEKKMVTLNNEVIPFYLTKLNVI 151
Score = 29.1 bits (62), Expect = 0.089
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = +3
Query: 84 MPNVKFYYFPVKALGE 131
MP+ K YYF VKALGE
Sbjct: 16 MPDYKVYYFNVKALGE 31
>AF316635-1|AAG45163.1| 224|Anopheles gambiae glutathione
S-transferase E1 protein.
Length = 224
Score = 35.5 bits (78), Expect = 0.001
Identities = 21/52 (40%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = +2
Query: 179 NRISSENW-PEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYGLAGAND 331
N ++ EN PEF P +PVL+ +G ++S AI YL RKYG D
Sbjct: 35 NLLAGENLTPEFLKLNPKHTIPVLDDNGTIISESHAIMIYLVRKYGQGEGKD 86
>AY063776-1|AAL59658.1| 224|Anopheles gambiae glutathione
S-transferase E1 protein.
Length = 224
Score = 34.7 bits (76), Expect = 0.002
Identities = 29/82 (35%), Positives = 37/82 (45%), Gaps = 4/82 (4%)
Frame = +2
Query: 98 VLLFPRQGPRRAASRLLLAYGGQEFED---NRISSENW-PEFKPKTPFGQMPVLEIDGKQ 265
VL R P A L G E E N ++ +N PEF P +PVL+ +G
Sbjct: 5 VLYTVRLSPPCRAVELTAKALGLELERKLVNLLAGQNLTPEFLKLNPKHTIPVLDDNGTI 64
Query: 266 YAQSTAICRYLGRKYGLAGAND 331
++S AI YL RKYG D
Sbjct: 65 ISESHAIMIYLVRKYGQGEGKD 86
>Z81291-1|CAB03592.1| 209|Anopheles gambiae GSTD1-5 protein
protein.
Length = 209
Score = 31.5 bits (68), Expect = 0.017
Identities = 23/71 (32%), Positives = 28/71 (39%), Gaps = 3/71 (4%)
Frame = +2
Query: 110 PRQGPRRAASRLLLAYGGQ---EFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQST 280
P P RA A G + + D PEF P +P L +G +S
Sbjct: 7 PGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKINPQHCIPTLVDNGFALWESR 66
Query: 281 AICRYLGRKYG 313
AIC YL KYG
Sbjct: 67 AICTYLAEKYG 77
>AF071160-3|AAC79993.1| 209|Anopheles gambiae glutathione
S-transferase protein.
Length = 209
Score = 31.5 bits (68), Expect = 0.017
Identities = 23/71 (32%), Positives = 28/71 (39%), Gaps = 3/71 (4%)
Frame = +2
Query: 110 PRQGPRRAASRLLLAYGGQ---EFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQST 280
P P RA A G + + D PEF P +P L +G +S
Sbjct: 7 PGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKINPQHCIPTLVDNGFALWESR 66
Query: 281 AICRYLGRKYG 313
AIC YL KYG
Sbjct: 67 AICTYLAEKYG 77
>AF515521-1|AAM61888.1| 233|Anopheles gambiae glutathione
S-transferase u1 protein.
Length = 233
Score = 30.7 bits (66), Expect = 0.029
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +2
Query: 206 EFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKY 310
E++ P ++PVL+ DG ++S AI +YL KY
Sbjct: 42 EYEKMNPQKEIPVLDDDGFFLSESNAILQYLCEKY 76
>AY255857-1|AAP13483.1| 216|Anopheles gambiae glutathione
tranferase d9 protein.
Length = 216
Score = 29.9 bits (64), Expect = 0.051
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = +2
Query: 203 PEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYGLAG 322
P FK P +P L +DG + AI YL +Y AG
Sbjct: 41 PAFKKINPQHTVPTLVVDGVAICEPGAILIYLAEQYAPAG 80
>AF316636-1|AAG45164.1| 221|Anopheles gambiae glutathione
S-transferase E2 protein.
Length = 221
Score = 29.9 bits (64), Expect = 0.051
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = +2
Query: 203 PEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYG 313
PEF P +PVL+ +G +S AI YL KYG
Sbjct: 44 PEFVKLNPQHTIPVLDDNGTIITESHAIMIYLVTKYG 80
>AY070256-1|AAL59655.1| 227|Anopheles gambiae glutathione
S-transferase E6 protein.
Length = 227
Score = 29.1 bits (62), Expect = 0.089
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = +2
Query: 206 EFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYG 313
EFK P +P L+ +G S AI YL R+YG
Sbjct: 46 EFKKLNPVQTIPTLDDNGFVLWDSHAIMIYLARRYG 81
>AF515522-1|AAM61889.1| 222|Anopheles gambiae glutathione
S-transferase protein.
Length = 222
Score = 29.1 bits (62), Expect = 0.089
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +2
Query: 206 EFKPKTPFGQMPVLEIDGKQYAQSTAICRYL 298
E++ P Q+P L+IDG +S +I YL
Sbjct: 55 EYREVNPMEQVPALQIDGHTLIESVSIMYYL 85
>Z71481-1|CAA96105.1| 140|Anopheles gambiae GSTD2 protein protein.
Length = 140
Score = 27.5 bits (58), Expect = 0.27
Identities = 22/71 (30%), Positives = 27/71 (38%), Gaps = 3/71 (4%)
Frame = +2
Query: 110 PRQGPRRAASRLLLAYGGQ---EFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQST 280
P P RA A G + + D PEF P +P L +G +S
Sbjct: 7 PGSAPCRAVQMTAAAVGAELNLKLTDLMKGEHMKPEFLKLNPQHCIPTLVDNGFALWESR 66
Query: 281 AICRYLGRKYG 313
AI YL KYG
Sbjct: 67 AIQIYLAEKYG 77
>AF515526-1|AAM61893.1| 229|Anopheles gambiae glutathione
S-transferase protein.
Length = 229
Score = 27.1 bits (57), Expect = 0.36
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +2
Query: 206 EFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKY 310
EFK F ++P + + A+S AI RYL R+Y
Sbjct: 46 EFKAINRFQKVPCITDSQIKLAESVAIFRYLCREY 80
>AF071162-1|AAC79998.1| 216|Anopheles gambiae glutathione
S-transferase D1-4 protein.
Length = 216
Score = 27.1 bits (57), Expect = 0.36
Identities = 24/77 (31%), Positives = 28/77 (36%), Gaps = 3/77 (3%)
Frame = +2
Query: 110 PRQGPRRAASRLLLAYGGQ---EFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQST 280
P P RA A G + + D PEF P +P L G +S
Sbjct: 7 PGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKLNPQHCVPTLVDSGFALWESR 66
Query: 281 AICRYLGRKYGLAGAND 331
AI YL KYG ND
Sbjct: 67 AIMCYLVEKYGKPCNND 83
>AF071160-2|AAC79994.1| 216|Anopheles gambiae glutathione
S-transferase protein.
Length = 216
Score = 27.1 bits (57), Expect = 0.36
Identities = 24/77 (31%), Positives = 28/77 (36%), Gaps = 3/77 (3%)
Frame = +2
Query: 110 PRQGPRRAASRLLLAYGGQ---EFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQST 280
P P RA A G + + D PEF P +P L G +S
Sbjct: 7 PGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKLNPQHCVPTLVDSGFALWESR 66
Query: 281 AICRYLGRKYGLAGAND 331
AI YL KYG ND
Sbjct: 67 AIMCYLVEKYGKPCNND 83
>Z81292-1|CAB03593.1| 209|Anopheles gambiae GSTD1-6 protein
protein.
Length = 209
Score = 26.2 bits (55), Expect = 0.63
Identities = 22/71 (30%), Positives = 27/71 (38%), Gaps = 3/71 (4%)
Frame = +2
Query: 110 PRQGPRRAASRLLLAYGGQ---EFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQST 280
P P RA A G + + D PEF P +P L +G +S
Sbjct: 7 PGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKLNPQHCIPTLVDNGFALWESR 66
Query: 281 AICRYLGRKYG 313
AI YL KYG
Sbjct: 67 AIQIYLAEKYG 77
>AF071160-1|AAC79995.1| 209|Anopheles gambiae glutathione
S-transferase protein.
Length = 209
Score = 26.2 bits (55), Expect = 0.63
Identities = 22/71 (30%), Positives = 27/71 (38%), Gaps = 3/71 (4%)
Frame = +2
Query: 110 PRQGPRRAASRLLLAYGGQ---EFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQST 280
P P RA A G + + D PEF P +P L +G +S
Sbjct: 7 PGSAPCRAVQMTAAAVGVELNLKLTDLMKGEHMKPEFLKLNPQHCIPTLVDNGFALWESR 66
Query: 281 AICRYLGRKYG 313
AI YL KYG
Sbjct: 67 AIQIYLAEKYG 77
>AY255856-1|AAP13482.1| 248|Anopheles gambiae glutathione
transferase o1 protein.
Length = 248
Score = 25.4 bits (53), Expect = 1.1
Identities = 11/22 (50%), Positives = 15/22 (68%), Gaps = 1/22 (4%)
Frame = +2
Query: 203 PE-FKPKTPFGQMPVLEIDGKQ 265
PE + K P G++P LEI GK+
Sbjct: 58 PEWYLEKNPLGKVPALEIPGKE 79
>AY578799-1|AAT07304.1| 679|Anopheles gambiae brinker protein.
Length = 679
Score = 25.0 bits (52), Expect = 1.4
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +3
Query: 249 KSTASSTLRAPPSAGTSVAST 311
KS AS T R+P +AGT+ +T
Sbjct: 14 KSEASQTGRSPSAAGTATTTT 34
>AY070255-1|AAL59654.1| 230|Anopheles gambiae glutathione
S-transferase E5 protein.
Length = 230
Score = 25.0 bits (52), Expect = 1.4
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = +2
Query: 206 EFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYGLAG 322
EF P +PV++ G S AI YL +KYG G
Sbjct: 48 EFLRLNPQHTIPVIDDGGVIVRDSHAIIIYLVQKYGKDG 86
>AF203335-1|AAF19830.1| 175|Anopheles gambiae immune-responsive
serine protease-relatedprotein ISPR20 protein.
Length = 175
Score = 24.2 bits (50), Expect = 2.5
Identities = 15/40 (37%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = -2
Query: 388 TGANVVQELHVLVDLEGLLVIGPGEPVLATEVP--ADGGA 275
+GAN++ H L D L+ EP AT +P DG A
Sbjct: 32 SGANIIDIRHPLDDCNDHLMQCCAEPKQATTIPPITDGDA 71
>AF515471-1|AAM61879.1| 225|Anopheles gambiae glutathione
S-transferase 3-8 protein.
Length = 225
Score = 23.8 bits (49), Expect = 3.3
Identities = 15/36 (41%), Positives = 17/36 (47%)
Frame = +2
Query: 206 EFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYG 313
EF P +PVL+ G S AI YL KYG
Sbjct: 47 EFLKVNPQQTIPVLDDGGIVITASHAITIYLVCKYG 82
>AF491816-1|AAM09542.2| 225|Anopheles gambiae glutathione
S-transferase E7 protein.
Length = 225
Score = 23.8 bits (49), Expect = 3.3
Identities = 15/36 (41%), Positives = 17/36 (47%)
Frame = +2
Query: 206 EFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYG 313
EF P +PVL+ G S AI YL KYG
Sbjct: 47 EFLKVNPQQTIPVLDDGGIVITASHAITIYLVCKYG 82
>AF316637-1|AAG45165.1| 224|Anopheles gambiae glutathione
S-transferase D8 protein.
Length = 224
Score = 23.8 bits (49), Expect = 3.3
Identities = 16/49 (32%), Positives = 21/49 (42%)
Frame = +2
Query: 167 EFEDNRISSENWPEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYG 313
+F D + P F PF +P L + +S AI YL KYG
Sbjct: 29 QFVDLMKDEQLRPTFTVLNPFHCVPTLVDNDLTMWESRAILVYLVDKYG 77
>AF513637-1|AAM53609.1| 214|Anopheles gambiae glutathione
S-transferase D11 protein.
Length = 214
Score = 23.4 bits (48), Expect = 4.4
Identities = 14/37 (37%), Positives = 17/37 (45%)
Frame = +2
Query: 203 PEFKPKTPFGQMPVLEIDGKQYAQSTAICRYLGRKYG 313
PEF P +P L + +S AI YL KYG
Sbjct: 41 PEFLKINPQHTVPTLVDNDFVLWESRAILTYLCEKYG 77
Database: mosquito
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 563,979
Number of sequences in database: 2352
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 442,170
Number of Sequences: 2352
Number of extensions: 7559
Number of successful extensions: 58
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 56
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 58
length of database: 563,979
effective HSP length: 60
effective length of database: 422,859
effective search space used: 44823054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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