Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-1850
         (750 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr...    32   0.076
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc...    27   2.9  
SPAC56F8.14c |mug115||sequence orphan|Schizosaccharomyces pombe|...    27   3.8  
SPAC144.03 |ade2|min10, min3|adenylosuccinate synthetase Ade2|Sc...    26   6.6  
SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding...    25   8.7  

>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 512

 Score = 32.3 bits (70), Expect = 0.076
 Identities = 37/174 (21%), Positives = 72/174 (41%), Gaps = 5/174 (2%)
 Frame = +2

Query: 134 LAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYPITGVDPLFKLHEIKSFFKLTQ 313
           L + + +LG+K GD + +A  N L+  + +YA          ++  +K  E + +    +
Sbjct: 40  LQRQIASLGIKVGDPVNIAIPNGLEFVVAFYAVSWQRAICGPLNSNYKQSEFEFYIDDLK 99

Query: 314 PKIAFCQQNQ---REXYLEAARELGLDTRVITFDGDEPMSKLLXXXXXXXXXXXQPATFD 484
            K+    +         + AA++L +    + +    P S+L+            P    
Sbjct: 100 SKLVIVPEGSVAANTPAVRAAKKLSVAVAELAWC---PKSRLVRIVHFEGAKINAPQPLG 156

Query: 485 LXKV--YVWLISTGGTSGVLKVAAIKHKVWIXKANCLTLGLFELKDKDDTSQVI 640
           L +    + ++ T GT+G  KV  + HK      + +T   + L D  DTS V+
Sbjct: 157 LPQPDDVMLVLHTSGTTGRPKVVPLTHKNLCRSIHNITTS-YRL-DPRDTSYVV 208


>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 762

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 22/78 (28%), Positives = 37/78 (47%), Gaps = 4/78 (5%)
 Frame = +2

Query: 80  AATGETETNASVLQRSVR---LAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYP 250
           A TG+T+  A    RS R   L  +  ++ L   D   +  +++    IP  + L N   
Sbjct: 466 ARTGKTDGAAHFTIRSSRELDLTSFDDSISLGFDDATNIRVKDNKRYQIPVISLLCNFVR 525

Query: 251 ITGVDPLF-KLHEIKSFF 301
            +G+DP F  L ++K+ F
Sbjct: 526 SSGMDPTFLDLWDVKTLF 543


>SPAC56F8.14c |mug115||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 131

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 13/33 (39%), Positives = 19/33 (57%)
 Frame = +1

Query: 10  QYHMGTSIYGLHEKTT*FSVPDRRSNRGDRDKR 108
           + H+G++ +GL E  T    P+RR N G   KR
Sbjct: 73  EIHLGSTEWGLKESFTLTRGPNRRGNVGKGVKR 105


>SPAC144.03 |ade2|min10, min3|adenylosuccinate synthetase
           Ade2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 434

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 21/86 (24%), Positives = 40/86 (46%), Gaps = 4/86 (4%)
 Frame = +2

Query: 47  RRRPDSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGR-NHLDLYIPY 223
           +R  +S+  + A T          ++   +  +++++G + G       R   LDL +  
Sbjct: 266 QRIANSIGVVKAYTTRVGAGPFPTEQLNEIGDHLQSVGREVGVTTGRKRRCGWLDLVVVK 325

Query: 224 YAALMNGYP---ITGVDPLFKLHEIK 292
           Y+ ++NGY    +T +D L  L EIK
Sbjct: 326 YSTMINGYTSLNLTKLDILDALDEIK 351


>SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding
           protein Ssb2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 279

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +1

Query: 73  DRRSNRGDRDKRVSTTAVCAVSQVHADA 156
           DR+   G++ + V+   +   SQVHADA
Sbjct: 41  DRQQGAGNKLRPVTIKQILNASQVHADA 68


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,573,335
Number of Sequences: 5004
Number of extensions: 43961
Number of successful extensions: 111
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 110
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)