Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1803
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 140 2e-34
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 51 2e-07
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 33 0.036
SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual 28 1.3
SPCC794.08 |||HEAT repeat protein, unknown biological role|Schiz... 27 2.3
SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual 27 3.1
SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch... 27 3.1
SPCC18.12c |||rRNA processing protein|Schizosaccharomyces pombe|... 27 4.1
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 26 5.4
SPBC23G7.04c |nif1||SEL1 repear protein Nif1|Schizosaccharomyces... 25 9.5
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 25 9.5
SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 compon... 25 9.5
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 140 bits (339), Expect = 2e-34
Identities = 69/173 (39%), Positives = 107/173 (61%), Gaps = 5/173 (2%)
Frame = +2
Query: 107 SASPITTTTTVIGVKFDKGCVIAGDTLGSYGSLARFRDCPRVMKVNDLILLGCGGDYADF 286
+ PI T ++V+ +KF G +IA D L SYGSLARF D R+ KV D ++G GGD +D+
Sbjct: 36 TVQPIVTGSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDY 95
Query: 287 QYLKDIIQQKIIDERCVGDGLQLKPRSLHCWLTRVLYNKRSKMDPLWNSYVVAGI--QDG 460
Q ++ ++++ I E GDG L+P +H +L++VLY +R+K+DP WN +VAG+ ++
Sbjct: 96 QQIQRLLEKLEIKEGNYGDGYALQPSYIHEYLSKVLYARRNKLDPYWNQLIVAGVDGENK 155
Query: 461 EPFLGAVDKLGTAYEDAVISNGLGAYMATPLLRDAVDKG---PLDQETAIAVV 610
EP++ D GT Y I+ G ++A P+LR A D L +E+A A +
Sbjct: 156 EPYVAFADLRGTTYSAPAIATGFAMHLALPMLRKATDDDRWKTLSKESARATI 208
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 51.2 bits (117), Expect = 2e-07
Identities = 43/147 (29%), Positives = 69/147 (46%), Gaps = 1/147 (0%)
Frame = +2
Query: 134 TVIGVKFDKGCVIAGDTLGSYGSLARFRDCPRVMKVNDLILLGCGGDYADFQYLKDIIQQ 313
T + + D ++AGDT G R PRV +V D +++G G AD L IQQ
Sbjct: 15 TTVAIAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADALALVKRIQQ 74
Query: 314 KIIDERCVGDGLQLKPRSLHCWLTRVLYNKRSKMDPLWNSYVVAGI-QDGEPFLGAVDKL 490
+ ID ++ +S C + +LY KR P + VAGI ++G+ + + D +
Sbjct: 75 R-IDLYHDNHERKMSAQSCACMVRTLLYGKR--FFPYYVYTTVAGIDKEGKGEIYSFDPV 131
Query: 491 GTAYEDAVISNGLGAYMATPLLRDAVD 571
G+ + + G A TP L + V+
Sbjct: 132 GSYEREWCRAGGSAANFITPFLDNQVN 158
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 33.5 bits (73), Expect = 0.036
Identities = 18/67 (26%), Positives = 35/67 (52%), Gaps = 1/67 (1%)
Frame = +2
Query: 122 TTTTTVIGVKFDKGCVIAG-DTLGSYGSLARFRDCPRVMKVNDLILLGCGGDYADFQYLK 298
+T TT++GV K C++ G DT + G + ++C ++ ++ I G AD +++
Sbjct: 33 STGTTIVGV-IAKDCIVLGADTRATAGPIIADKNCKKLHLISPNIWCAGAGTAADTEFVT 91
Query: 299 DIIQQKI 319
+I I
Sbjct: 92 SMISSNI 98
>SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual
Length = 935
Score = 28.3 bits (60), Expect = 1.3
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +2
Query: 35 FYNFPGNASTIAPSRHGVQDFTAHSASPITTTTTVI 142
+YN G +S + + HG+ DF +H + T T I
Sbjct: 254 YYNNDGASSWVFTADHGMSDFGSHGDGNLDNTRTPI 289
>SPCC794.08 |||HEAT repeat protein, unknown biological
role|Schizosaccharomyces pombe|chr 3|||Manual
Length = 798
Score = 27.5 bits (58), Expect = 2.3
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -2
Query: 514 HSIFICCSQFINSSEKGFTILNTGDHVAVPERIH 413
HSIF CC + ++SS I+N+ +V VP I+
Sbjct: 309 HSIFFCCLRCLSSSR----IVNSETNVMVPYMIY 338
>SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 533
Score = 27.1 bits (57), Expect = 3.1
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +3
Query: 99 QLTQRAPSRPPQRSSELSLTRDVLSPVTHWART 197
+L +R P RPP R +++ + +LS + W +T
Sbjct: 92 ELVRRFPERPPSRLNKIQVM--ILSLIEEWRKT 122
>SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase
Ubp14|Schizosaccharomyces pombe|chr 2|||Manual
Length = 775
Score = 27.1 bits (57), Expect = 3.1
Identities = 15/46 (32%), Positives = 23/46 (50%)
Frame = +3
Query: 27 LEHFIIFLEMLPQLLHPGTVYRILQLTQRAPSRPPQRSSELSLTRD 164
L+H ++ E Q LHP V Q Q+ PPQ+ ++L + D
Sbjct: 54 LKHSLVHFE---QTLHPIVVTIARQPKQKINDEPPQKITKLEIRED 96
>SPCC18.12c |||rRNA processing protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 260
Score = 26.6 bits (56), Expect = 4.1
Identities = 16/47 (34%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
Frame = +2
Query: 281 DFQYLKDIIQQKIIDERCVGDGLQ--LKPRSLHCWLTRVLYNKRSKM 415
D +LKD+ QQKI + + +Q +KP C + R LY+K ++
Sbjct: 31 DADFLKDLSQQKIDIQAALARTVQGAIKPMITQCCI-RQLYSKSDEL 76
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 26.2 bits (55), Expect = 5.4
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +2
Query: 164 CVIAGDTLGSYGSLARFRDCPRVMKVNDLILL 259
C AG +LG Y +L+ D + + DL+ L
Sbjct: 1822 CAFAGHSLGEYSALSAMGDVLSIEALVDLVFL 1853
>SPBC23G7.04c |nif1||SEL1 repear protein Nif1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 681
Score = 25.4 bits (53), Expect = 9.5
Identities = 14/46 (30%), Positives = 24/46 (52%)
Frame = -3
Query: 675 PELVALERASISVEKXPPXLLRTTAIAVSWSRGPLSTASLSRGVAM 538
P+ A R++ S +K P +++ S PLS +S S+G+ M
Sbjct: 211 PKRPAFTRSATSPDKILPTRIKSKDTVSSGDSTPLSGSSSSKGMLM 256
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 25.4 bits (53), Expect = 9.5
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = -1
Query: 548 ESPCKLLARSRSQHLHMLFPIYQQ 477
E+ KLLA+S ++H+ LF YQ+
Sbjct: 585 ENVAKLLAQSTNKHVATLFSDYQE 608
>SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 component
of oxoglutarate dehydrogenase complex
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.4 bits (53), Expect = 9.5
Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = +2
Query: 539 MATPLLRDAVDKGPLDQETAIAVV-RKSXGGXFSTEMLARSNATSS 673
+ TP++R+A L+ E+AIA + K+ G + E +A T S
Sbjct: 327 LVTPVIRNAESMSLLEIESAIATLGSKARAGKLAIEDMASGTFTIS 372
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,249,638
Number of Sequences: 5004
Number of extensions: 64677
Number of successful extensions: 202
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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