Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1746
(750 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 462 e-132
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 450 e-129
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 343 8e-97
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 310 7e-87
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 1e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.3
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.1
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.1
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.0
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 9.3
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 9.3
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 462 bits (1138), Expect = e-132
Identities = 217/226 (96%), Positives = 219/226 (96%)
Frame = +3
Query: 48 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 227
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 228 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 407
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 408 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 587
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 588 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQXERKEGKADRK 725
IGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW ERKEGK + K
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGK 226
Score = 26.2 bits (55), Expect = 0.33
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +1
Query: 703 RKAKLTGKCLIEALD 747
++ K+ GKCLIEALD
Sbjct: 219 KEGKVEGKCLIEALD 233
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 450 bits (1110), Expect = e-129
Identities = 210/226 (92%), Positives = 216/226 (95%)
Frame = +3
Query: 48 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 227
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 228 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 407
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 408 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 587
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 588 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQXERKEGKADRK 725
IGYN A+VAFVPISGWHGDNMLEPS K PW+KGW+ ERK+G AD K
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGK 226
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 343 bits (844), Expect = 8e-97
Identities = 161/169 (95%), Positives = 163/169 (96%)
Frame = +3
Query: 219 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 398
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 399 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 578
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 579 IKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQXERKEGKADRK 725
IKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW ERKEGK + K
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGK 169
Score = 26.2 bits (55), Expect = 0.33
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +1
Query: 703 RKAKLTGKCLIEALD 747
++ K+ GKCLIEALD
Sbjct: 162 KEGKVEGKCLIEALD 176
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 310 bits (762), Expect = 7e-87
Identities = 145/153 (94%), Positives = 147/153 (96%)
Frame = +3
Query: 267 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 446
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 447 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 626
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 627 SGWHGDNMLEPSTKMPWFKGWQXERKEGKADRK 725
SGWHGDNMLE S+KMPWFKGW ERKEGK + K
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGK 153
Score = 26.2 bits (55), Expect = 0.33
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +1
Query: 703 RKAKLTGKCLIEALD 747
++ K+ GKCLIEALD
Sbjct: 146 KEGKVEGKCLIEALD 160
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 1e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +3
Query: 306 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 485
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 486 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 656
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.57
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +3
Query: 60 KTHINIVVIGHVDSGKST 113
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.3
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +3
Query: 69 INIVVIGHVDSGKST 113
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.1
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 61 FSLPIFG*SRITNCV*Y 11
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.1
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 291 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 392
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.0
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 291 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 392
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 21.4 bits (43), Expect = 9.3
Identities = 8/24 (33%), Positives = 13/24 (54%)
Frame = -2
Query: 668 FG*RLQHVVSVPSRNGHESDSSWV 597
FG L H++ V +N + + WV
Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 21.4 bits (43), Expect = 9.3
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +1
Query: 511 WIPLNHHTVSPDLRKSRRKY 570
W+P+N + S +L +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 216,912
Number of Sequences: 438
Number of extensions: 4525
Number of successful extensions: 21
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23510295
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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