Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1745
(751 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 69 7e-13
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 65 9e-12
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 63 4e-11
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 63 4e-11
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 59 8e-10
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 58 1e-09
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 54 2e-08
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 54 3e-08
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 52 7e-08
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 50 3e-07
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 47 3e-06
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 40 5e-04
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 36 0.008
SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce... 27 3.8
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 27 3.8
SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin Nup132|Schizosa... 25 8.7
SPBC12C2.03c |||FAD binding protein |Schizosaccharomyces pombe|c... 25 8.7
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 25 8.7
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 68.9 bits (161), Expect = 7e-13
Identities = 36/80 (45%), Positives = 44/80 (55%)
Frame = +2
Query: 95 QYTWXGVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSILTD 274
Q T G + Y G F L F YPF P+V F HP++ SNG ICL IL D
Sbjct: 34 QATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIY--HPNINSNGSICLDILRD 91
Query: 275 DWSPALSVQSVCLSIVSMLS 334
WSPAL++ V LSI S+L+
Sbjct: 92 QWSPALTISKVLLSICSLLT 111
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 65.3 bits (152), Expect = 9e-12
Identities = 33/77 (42%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
Frame = +2
Query: 110 GVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHV-YSNGHICLSILTDDWSP 286
G T YEG ++VL YP P V F VHP++ ++NG +C+ IL WSP
Sbjct: 43 GPTETPYEGGQWVLDIHVHEGYPISPPSVYF--QTKIVHPNISWTNGEVCMDILKTHWSP 100
Query: 287 ALSVQSVCLSIVSMLSS 337
A S+QS CL+I+S+LS+
Sbjct: 101 AWSLQSACLAIISLLSN 117
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 63.3 bits (147), Expect = 4e-11
Identities = 33/83 (39%), Positives = 47/83 (56%)
Frame = +2
Query: 89 YGQYTWXGVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSIL 268
Y + T G + YEG KF L+ ++YP P V F+ HP+V G ICLS L
Sbjct: 33 YFKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIY--HPNVDKLGRICLSTL 90
Query: 269 TDDWSPALSVQSVCLSIVSMLSS 337
DWSPAL +++V LSI +++ +
Sbjct: 91 KKDWSPALQIRTVLLSIQALMGA 113
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 63.3 bits (147), Expect = 4e-11
Identities = 37/105 (35%), Positives = 55/105 (52%), Gaps = 1/105 (0%)
Frame = +2
Query: 101 TWXGVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPV-HPHVYSNGHICLSILTDD 277
T G T YEG KF + F YP+ P + F P+ HP+V +G+ICL IL D
Sbjct: 64 TITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTS---PMWHPNVDMSGNICLDILKDK 120
Query: 278 WSPALSVQSVCLSIVSMLSSCKEKRRPPDNSFYVKTCNKNPKKTK 412
WS +VQ++ LS+ S+L + P N+ + +K+P + K
Sbjct: 121 WSAVYNVQTILLSLQSLLG--EPNNASPLNAQAAELWSKDPIEYK 163
Score = 25.4 bits (53), Expect = 8.7
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +1
Query: 1 RLQKXLMSLMKEPPPGVTVDGDQASQNLTLW 93
RL+ LMSLM PG++ D S NL W
Sbjct: 32 RLRSELMSLMMSNTPGISAFPDSDS-NLLHW 61
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 58.8 bits (136), Expect = 8e-10
Identities = 34/86 (39%), Positives = 47/86 (54%), Gaps = 1/86 (1%)
Frame = +2
Query: 110 GVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSN-GHICLSILTDDWSP 286
G GT YEG FV+ + YPF P++ F + HP+V S G ICL IL D WSP
Sbjct: 42 GPEGTPYEGGYFVVDIEIPIDYPFRPPKMNF--DTKIYHPNVSSQTGAICLDILKDQWSP 99
Query: 287 ALSVQSVCLSIVSMLSSCKEKRRPPD 364
+++S +S+ S+L + E P D
Sbjct: 100 VYTMKSALISLQSLLCT-PEPSNPQD 124
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 58.0 bits (134), Expect = 1e-09
Identities = 29/75 (38%), Positives = 40/75 (53%)
Frame = +2
Query: 110 GVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSILTDDWSPA 289
G T +E F L F +YP P V F+ HP+VY+NG +CL IL + WSP
Sbjct: 42 GPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMF--HPNVYANGELCLDILQNRWSPT 99
Query: 290 LSVQSVCLSIVSMLS 334
V ++ SI S+L+
Sbjct: 100 YDVAAILTSIQSLLN 114
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 54.0 bits (124), Expect = 2e-08
Identities = 23/64 (35%), Positives = 40/64 (62%)
Frame = +2
Query: 128 YEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSILTDDWSPALSVQSV 307
Y+G KF + + + YP D P+V + N I HP++ G++CL+IL DW+P L++ S+
Sbjct: 65 YKGGKFKFRIQIDDNYPHDPPKVKCL-NKI-YHPNIDIEGNVCLNILRQDWNPVLNLNSI 122
Query: 308 CLSI 319
+ +
Sbjct: 123 LVGL 126
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 53.6 bits (123), Expect = 3e-08
Identities = 27/76 (35%), Positives = 42/76 (55%), Gaps = 2/76 (2%)
Frame = +2
Query: 110 GVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSILTDD--WS 283
G P T +EG + L F +YP P+ F HP+VY +G +CLSIL ++ W
Sbjct: 47 GKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLF--HPNVYPSGTVCLSILNEEEGWK 104
Query: 284 PALSVQSVCLSIVSML 331
PA++++ + L I +L
Sbjct: 105 PAITIKQILLGIQDLL 120
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 52.4 bits (120), Expect = 7e-08
Identities = 38/114 (33%), Positives = 55/114 (48%), Gaps = 13/114 (11%)
Frame = +2
Query: 110 GVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSIL---TDD- 277
G TLYEG F F YP P++ F HP+V+ NG +C+SIL DD
Sbjct: 44 GPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIW--HPNVHPNGEVCISILHPPGDDK 101
Query: 278 ---------WSPALSVQSVCLSIVSMLSSCKEKRRPPDNSFYVKTCNKNPKKTK 412
W P S +++ +S++SMLSS ++ P N K +NP++ K
Sbjct: 102 YGYEDAGERWLPVHSPETILISVISMLSSPNDE--SPANIDAAKEFRENPQEFK 153
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 50.4 bits (115), Expect = 3e-07
Identities = 34/96 (35%), Positives = 48/96 (50%), Gaps = 17/96 (17%)
Frame = +2
Query: 98 YTWX----GVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSI 265
+TW G GT +EG + KF + YP P + F HP+VY +G +C+SI
Sbjct: 36 FTWDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKFECEFF--HPNVYKDGTVCISI 93
Query: 266 LT---DD----------WSPALSVQSVCLSIVSMLS 334
L DD WSP SV+ + LS++SML+
Sbjct: 94 LHAPGDDPNMYESSSERWSPVQSVEKILLSVMSMLA 129
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 46.8 bits (106), Expect = 3e-06
Identities = 26/78 (33%), Positives = 40/78 (51%), Gaps = 1/78 (1%)
Frame = +2
Query: 101 TWXGVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSILTDD- 277
T G ++Y G KF KF YPF P + F HP+ S G++CL+IL
Sbjct: 42 TALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEF--TTRIYHPNFDSEGNVCLAILKQQV 99
Query: 278 WSPALSVQSVCLSIVSML 331
+ P++ ++SV I+ +L
Sbjct: 100 FKPSIKLRSVLEQILQLL 117
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 39.5 bits (88), Expect = 5e-04
Identities = 19/67 (28%), Positives = 36/67 (53%), Gaps = 1/67 (1%)
Frame = +2
Query: 110 GVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYS-NGHICLSILTDDWSP 286
G T Y G + + + ++YP+ SP + F+ N I HP++ +G +CL ++ WSP
Sbjct: 38 GPSETPYSGGIWKVHVELPSEYPWKSPSIGFV-NRI-FHPNIDELSGSVCLDVINQTWSP 95
Query: 287 ALSVQSV 307
+ ++
Sbjct: 96 MFDMINI 102
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 35.5 bits (78), Expect = 0.008
Identities = 23/83 (27%), Positives = 37/83 (44%), Gaps = 3/83 (3%)
Frame = +2
Query: 98 YTWXGVPGTLYEGEKFVLQFKFTNKYPFDSPEVTFIGNNIPVHPHVYSNGHICLSIL--- 268
Y G P T YEG ++ F YPF P + I + +N +CLS
Sbjct: 39 YIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITPS----GRFQTNTRLCLSFSDFH 94
Query: 269 TDDWSPALSVQSVCLSIVSMLSS 337
W+P+ V ++ + +VS ++S
Sbjct: 95 PKSWNPSWMVSTILVGLVSFMTS 117
>SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 604
Score = 26.6 bits (56), Expect = 3.8
Identities = 15/47 (31%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
Frame = +2
Query: 491 DKCYNNEDVRCANESGTGRVERRTYCPQQ--GSKCPSQTRSYRTYRA 625
D YN++ V ++ T R RR+Y P+ GS+ PS S ++++
Sbjct: 96 DSGYNDDVVTNSSSHRTPRHHRRSYSPRSDYGSRSPSPHSSVDSHQS 142
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 26.6 bits (56), Expect = 3.8
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = +3
Query: 315 RSCRCSVAVRKRDDRLIIRSTLKPVIKILR 404
++C C ++ +K D+LI ST+K V I +
Sbjct: 43 KNCACVISQKKVPDKLIDASTVKHVFPITK 72
>SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin
Nup132|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1162
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = +3
Query: 333 VAVRKRDDRLIIRSTLKPVIKILRKQNGGIMMIRCSK 443
V V++ D ++ R++ + VI++ +GGI M C K
Sbjct: 382 VHVQEDDKDIVSRTSWEEVIRMNTNVSGGIFMSTCYK 418
>SPBC12C2.03c |||FAD binding protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 571
Score = 25.4 bits (53), Expect = 8.7
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +3
Query: 264 SSPTTGRRRCRCNQSASRSCRCS 332
SSP++ + C N++ +CRCS
Sbjct: 20 SSPSSNFKTCVSNENGCINCRCS 42
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 25.4 bits (53), Expect = 8.7
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 155 FKFTNKYPFDSPEVTFIGNNIPVHP 229
F+ N PFDS + G++IP+ P
Sbjct: 251 FRSANASPFDSCDSFHSGSSIPIEP 275
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,879,017
Number of Sequences: 5004
Number of extensions: 59116
Number of successful extensions: 198
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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