Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1739
(710 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 54 2e-08
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 39 6e-04
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 38 0.002
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 37 0.003
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 32 0.070
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 29 0.66
SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase Ubp12|Schizo... 29 0.87
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 28 1.1
SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces p... 28 1.5
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 27 3.5
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch... 26 4.6
SPAC1D4.06c |csk1||cyclin-dependent kinase activating kinase Csk... 26 6.1
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 25 8.1
SPBC30D10.17c |||glucan synthase regulator |Schizosaccharomyces ... 25 8.1
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 54.0 bits (124), Expect = 2e-08
Identities = 23/30 (76%), Positives = 26/30 (86%)
Frame = +2
Query: 533 EEHPVLLTEAPLNPKANREKMTQIMFRNIS 622
EEHP LLTEAPLNPK+NREKMTQI+F +
Sbjct: 99 EEHPCLLTEAPLNPKSNREKMTQIIFETFN 128
Score = 52.4 bits (120), Expect = 6e-08
Identities = 27/45 (60%), Positives = 30/45 (66%)
Frame = +1
Query: 562 SPQPQGQQREDDPDHVSKHFNTPAMYVAIQAVLSLYASGRTTGIV 696
+P RE + + FN PA YVAIQAVLSLYASGRTTGIV
Sbjct: 108 APLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIV 152
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 39.1 bits (87), Expect = 6e-04
Identities = 19/38 (50%), Positives = 23/38 (60%), Gaps = 3/38 (7%)
Frame = +2
Query: 518 FKYFN---EEHPVLLTEAPLNPKANREKMTQIMFRNIS 622
FKY E+H LLTE PLNP NRE +IMF + +
Sbjct: 114 FKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFN 151
Score = 30.7 bits (66), Expect = 0.21
Identities = 13/32 (40%), Positives = 22/32 (68%)
Frame = +1
Query: 580 QQREDDPDHVSKHFNTPAMYVAIQAVLSLYAS 675
+ RE+ + + + FN +Y+A+QAVL+L AS
Sbjct: 137 ENRENTAEIMFESFNCAGLYIAVQAVLALAAS 168
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 37.5 bits (83), Expect = 0.002
Identities = 17/39 (43%), Positives = 26/39 (66%)
Frame = +1
Query: 580 QQREDDPDHVSKHFNTPAMYVAIQAVLSLYASGRTTGIV 696
+ +E ++ + N PA+ ++Q VL+LYAS RTTGIV
Sbjct: 119 RNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIV 157
Score = 33.1 bits (72), Expect = 0.040
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = +2
Query: 533 EEHPVLLTEAPLNPKANREKMTQIMFRNIS 622
EEHP+LLTE PL N+EK+ + + ++
Sbjct: 104 EEHPLLLTEPPLANIRNKEKIAEYFYETLN 133
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 36.7 bits (81), Expect = 0.003
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +2
Query: 545 VLLTEAPLNPKANREKMTQIMF 610
+LLTE P+NP ANREKM + MF
Sbjct: 103 ILLTEPPMNPVANREKMCETMF 124
Score = 35.1 bits (77), Expect = 0.010
Identities = 17/37 (45%), Positives = 25/37 (67%)
Frame = +1
Query: 586 REDDPDHVSKHFNTPAMYVAIQAVLSLYASGRTTGIV 696
RE + + + + +YVAIQAVLSLYA G ++G+V
Sbjct: 116 REKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVV 152
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 32.3 bits (70), Expect = 0.070
Identities = 18/39 (46%), Positives = 22/39 (56%)
Frame = +2
Query: 536 EHPVLLTEAPLNPKANREKMTQIMFRNISTRPPCTSPSK 652
EHP+L+TE NP NR K + MF S R P T +K
Sbjct: 98 EHPILITEPFDNPPENRVKTLETMFE--SLRCPATYLAK 134
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 29.1 bits (62), Expect = 0.66
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +2
Query: 536 EHPVLLTEAPLNPKANREKMTQIMF 610
EHP+ +TE NP R MT+++F
Sbjct: 115 EHPICMTEPLANPTYVRSTMTELLF 139
>SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase
Ubp12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 979
Score = 28.7 bits (61), Expect = 0.87
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 3/38 (7%)
Frame = +1
Query: 301 CLINFRW*F---LRLPWLSRVTGNQGSIPEREPEKRLP 405
C+IN W LRL +L + NQ S E++ EKR+P
Sbjct: 271 CMINETWPVDRALRLQFLIQQRNNQSSNEEQKQEKRVP 308
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 28.3 bits (60), Expect = 1.1
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +2
Query: 542 PVLLTEAPLNPKANREKMTQIMFRNISTRPPCTSPSKP 655
P +++ PL P R + FR+ S +PP + P P
Sbjct: 42 PAPVSKKPLPPPTRRLPRKPLPFRSTSLQPPSSQPPAP 79
>SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 473
Score = 27.9 bits (59), Expect = 1.5
Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = +2
Query: 533 EEHPVLLTEAPL--NPKANREKMTQIMFRNISTRPPCT 640
E + +L +EAPL P ++ +M Q M + + PPCT
Sbjct: 211 ESNILLASEAPLFLTPAKSKAEMIQFMNQLADSAPPCT 248
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 26.6 bits (56), Expect = 3.5
Identities = 14/46 (30%), Positives = 21/46 (45%)
Frame = +1
Query: 334 LPWLSRVTGNQGSIPEREPEKRLPHPRKAAGAQITHSRHGEVVTKN 471
+P +S V + IPER+ + L P + H EVV +N
Sbjct: 757 VPCISGVMLHSKIIPERKNSEFLSFPTSQQECLLVHDNQAEVVVQN 802
>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 845
Score = 26.2 bits (55), Expect = 4.6
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = -1
Query: 512 LIPITRPRKSPVSLFFVTTSPCREWVICAPAAFL 411
+IPI + KS +SL F+T SPC + IC+ A L
Sbjct: 45 IIPIAQ--KSNISLPFLTLSPC-SFTICSLRARL 75
>SPAC1D4.06c |csk1||cyclin-dependent kinase activating kinase Csk1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 306
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = -3
Query: 414 PWMW*PFLRLPLRNRTLIPRYP*QPW*SQKLPS 316
P MW P N+ + YP +PW S+ LPS
Sbjct: 236 PSMWPELSTFPDWNKFIFHEYPPKPW-SEILPS 267
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/36 (30%), Positives = 24/36 (66%), Gaps = 2/36 (5%)
Frame = +2
Query: 524 YFNEEHPVLLTE--APLNPKANREKMTQIMFRNIST 625
++ E+HP+LLT+ A L P+ + ++ +I ++ +T
Sbjct: 1405 FYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENT 1440
>SPBC30D10.17c |||glucan synthase regulator |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 504
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -2
Query: 412 LDVVAVSQAPSPESNPDSPLPVTTMVVAETTIES 311
L ++ S+ P + PD P T+ V+ TT+E+
Sbjct: 422 LGLINTSEINQPANLPDEPTAETSNPVSATTVEA 455
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,044,709
Number of Sequences: 5004
Number of extensions: 64342
Number of successful extensions: 179
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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