Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1732
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 375 e-105
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 375 e-105
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 375 e-105
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 90 3e-19
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 77 2e-15
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 74 2e-14
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 34 0.017
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 1.1
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 2.6
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.4
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 4.5
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.0
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 26 6.0
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 7.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 375 bits (923), Expect = e-105
Identities = 167/227 (73%), Positives = 193/227 (85%)
Frame = +3
Query: 18 KIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPA 197
K+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPA
Sbjct: 178 KVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPA 237
Query: 198 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEAL 377
RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L
Sbjct: 238 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESL 297
Query: 378 QEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTP 557
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+P
Sbjct: 298 DAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSP 357
Query: 558 VLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGXAXIXNLVP 698
VLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSG A I +VP
Sbjct: 358 VLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVP 404
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 375 bits (923), Expect = e-105
Identities = 167/227 (73%), Positives = 193/227 (85%)
Frame = +3
Query: 18 KIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPA 197
K+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPA
Sbjct: 178 KVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPA 237
Query: 198 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEAL 377
RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L
Sbjct: 238 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESL 297
Query: 378 QEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTP 557
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+P
Sbjct: 298 DAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSP 357
Query: 558 VLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGXAXIXNLVP 698
VLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSG A I +VP
Sbjct: 358 VLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVP 404
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 375 bits (923), Expect = e-105
Identities = 167/227 (73%), Positives = 193/227 (85%)
Frame = +3
Query: 18 KIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPA 197
K+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G GK L+EA+D+I PPA
Sbjct: 178 KVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPA 237
Query: 198 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEAL 377
RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L
Sbjct: 238 RPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESL 297
Query: 378 QEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTP 557
+PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+P
Sbjct: 298 DAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSP 357
Query: 558 VLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGXAXIXNLVP 698
VLDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSG A I +VP
Sbjct: 358 VLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVP 404
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 89.8 bits (213), Expect = 3e-19
Identities = 61/209 (29%), Positives = 104/209 (49%), Gaps = 4/209 (1%)
Frame = +3
Query: 24 GYNPAA-VAFVPISGWHGDNMLE--PSTKMPWFKGWQVERKEGKADGKCLIEALDAILPP 194
GYN V ++P+S + G N+ + S+ PW++G L+E LD++
Sbjct: 414 GYNSKTDVKYMPVSAYTGQNVKDRVDSSVCPWYQG------------PSLLEYLDSMTHL 461
Query: 195 ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHE 371
R + P +P+ YK +GT+ G++E G +K + V+ P N T EV ++ + E
Sbjct: 462 ERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADE 519
Query: 372 ALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGY 551
+ ++ GD V V+ +++ GYV +KN P F AQ+ +L P ++ GY
Sbjct: 520 EISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGY 577
Query: 552 TPVLDCHTAHIACKFAEIKEKVDRRTGKS 638
+ V+ HTA FA++ K+D+ KS
Sbjct: 578 SCVMHIHTAVEEVSFAKLLHKLDKTNRKS 606
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 77.4 bits (182), Expect = 2e-15
Identities = 56/184 (30%), Positives = 90/184 (48%), Gaps = 3/184 (1%)
Frame = +3
Query: 21 IGYNPAAVAFVPISGWHGDNMLEPSTK--MPWFKGWQVERKEGKADGKCLIEALDAILPP 194
+G+ + V FVPIS G N+++ + W+KG L+ ALD ++PP
Sbjct: 350 VGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG------------PTLLSALDQLVPP 397
Query: 195 ARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEA 374
+P KPLRL + DVY+ TV GRVE G ++ ++ + VK+V + +
Sbjct: 398 EKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDP 456
Query: 375 LQE-AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGY 551
AV GD V + ++ V +LR G + + + NP + F A++ + G I +G
Sbjct: 457 SSTWAVAGDTVTLQLADIEVNQLRPGDILSNYE-NPVRRVRSFVAEIQTFDIHGPILSGS 515
Query: 552 TPVL 563
T VL
Sbjct: 516 TLVL 519
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 73.7 bits (173), Expect = 2e-14
Identities = 39/101 (38%), Positives = 61/101 (60%), Gaps = 3/101 (2%)
Frame = +3
Query: 162 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPAN 332
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G +V ++
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSH 293
Query: 333 ITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 455
+ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 294 LKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 34.3 bits (75), Expect = 0.017
Identities = 31/113 (27%), Positives = 49/113 (43%), Gaps = 5/113 (4%)
Frame = +3
Query: 102 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 281
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 282 ETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEA--VPGDNVGF---NVKNV 425
G +K G V+ EV+ V + + + E + VG+ N+KN+
Sbjct: 266 MEGFIKKGGKVMSVNTGRKYEVQQVGIMYPDMTEVSRLRAGQVGYIIWNMKNI 318
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 28.3 bits (60), Expect = 1.1
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -1
Query: 202 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 65
G GG S+ P+ LPS + P L E +ML CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 2.6
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -1
Query: 346 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 230
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.4
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 440 QFLDGHVLYVETYIVSRYSFLESFV 366
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 4.5
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = -1
Query: 304 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 140
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 139 SLRSTCHPLNQG 104
P G
Sbjct: 374 PADGVPKPTPDG 385
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 6.0
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -3
Query: 464 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 348
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 25.8 bits (54), Expect = 6.0
Identities = 17/67 (25%), Positives = 31/67 (46%)
Frame = -3
Query: 332 VGGGKDNNGTWFQHTSFNSADGHGTNTTDFVYVLQGKTQGLVSGAGRWQDGIESFNEAFS 153
VG D+ + + + + DF+ ++GK L + R+++ ES NE
Sbjct: 125 VGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNESTNERVK 184
Query: 152 VSFAFLT 132
+FA+LT
Sbjct: 185 -NFAYLT 190
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 7.9
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 295 NTPVSTLPTGTVPIPPIL 242
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,124,471
Number of Sequences: 5004
Number of extensions: 68906
Number of successful extensions: 240
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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