Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-1708
         (700 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC24H6.04 |hxk1||hexokinase 1 |Schizosaccharomyces pombe|chr 1...   113   3e-26
SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr 1...    80   3e-16
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    26   6.0  
SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|c...    26   6.0  
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|...    25   7.9  
SPAC644.13c |||Rab GTPase binding |Schizosaccharomyces pombe|chr...    25   7.9  

>SPAC24H6.04 |hxk1||hexokinase 1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 484

 Score =  113 bits (271), Expect = 3e-26
 Identities = 55/143 (38%), Positives = 81/143 (56%), Gaps = 1/143 (0%)
 Frame = +3

Query: 66  LRRVAEVFEKEIEAGLRQQPSSLQMENTYVPELPDGTEEGSFLALDLGGTNFRVLFLELK 245
           L RV + F  E+  GL   P  + M  T++   PDG E GS+LALDLGGTN RV  +E++
Sbjct: 45  LHRVTDRFVSELYKGLTTNPGDVPMVPTWIIGTPDGNEHGSYLALDLGGTNLRVCAVEVQ 104

Query: 246 AGQLLREKVKHYHINDALRLGPGEDLFNFLADCVQDFLVSQSMEK-ETFSLGFTFSFPMR 422
                      Y +   L++G  E LF+++ADC++ F+      K +   +GFTFS+P  
Sbjct: 105 GNGKFDITQSKYRLPQELKVGTREALFDYIADCIKKFVEEVHPGKSQNLEIGFTFSYPCV 164

Query: 423 QHSISSGELITWTKSFNCGGMDG 491
           Q SI+   L+ WTK F+  G++G
Sbjct: 165 QRSINDASLVAWTKGFDIDGVEG 187



 Score = 33.1 bits (72), Expect = 0.039
 Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 1/59 (1%)
 Frame = +1

Query: 490 GFDVAALLQRCLSARSLD-VTVRVLLNDTTGTLVAGAHIDRNVGIXXDSGYRLNRCYME 663
           G  V  LL   L     + V +  +L+DTTGTLVA  +      I    G   N CY+E
Sbjct: 187 GESVGPLLSAALKRVGCNNVRLNAILSDTTGTLVASNYASPGTEIGVIFGTGCNACYIE 245


>SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 455

 Score = 80.2 bits (189), Expect = 3e-16
 Identities = 47/144 (32%), Positives = 75/144 (52%), Gaps = 7/144 (4%)
 Frame = +3

Query: 63  TLRRVAEVFEKEIEAGLRQQPSSLQMENTYVPELPDGTEEGSFLALDLGGTNFRVLFLEL 242
           +LR   + F++  + GL++    L M   ++  LP G E G FLALD GGTN RV +++L
Sbjct: 22  SLREAVKEFDELRQKGLQKNGEVLAMAPAFISTLPTGAETGDFLALDFGGTNLRVCWIQL 81

Query: 243 KA-GQLLREKVKHYHINDALRLGPGEDLFNFLADCVQDFLVSQ------SMEKETFSLGF 401
              G+   +  K     + +R    + + +F++D V+ F+           E+E   +GF
Sbjct: 82  LGDGKYEMKHSKSVLPRECVRNESVKPIIDFMSDHVELFIKEHFPSKFGCPEEEYLPMGF 141

Query: 402 TFSFPMRQHSISSGELITWTKSFN 473
           TFS+P  Q SI+   L+ WTK  N
Sbjct: 142 TFSYPANQVSITESYLLRWTKGLN 165



 Score = 33.1 bits (72), Expect = 0.039
 Identities = 24/74 (32%), Positives = 35/74 (47%), Gaps = 9/74 (12%)
 Frame = +1

Query: 496 DVAALLQRCLSARSLDVTVRVLLNDTTGTLVAGAHI----DRNVGIXXDSGYRLNRCYME 663
           D A  L     AR+L + +  ++NDT GTLV  A+     D  +GI   +G   N  Y+E
Sbjct: 173 DFAQFLTEGFKARNLPIRIEAVINDTVGTLVTRAYTSKESDTFMGIIFGTG--TNGAYVE 230

Query: 664 -----PRVTGSSLG 690
                P++ G   G
Sbjct: 231 QMNQIPKLAGKCTG 244


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 18/54 (33%), Positives = 23/54 (42%)
 Frame = -2

Query: 279  SVSLSHGAIVRLSTPKKGPENLYHPNPTLGTSLPLFRQAILGHKYFPFASSKVV 118
            S++ S  AI     PK G    YHP    GT +P  +Q    + Y P A    V
Sbjct: 915  SMTASAPAIASPPPPKVG--ETYHPPTASGTRVPPVQQPSHPNPYTPVAPQSPV 966


>SPBC12C2.07c |||spermidine synthase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 298

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 31/137 (22%), Positives = 54/137 (39%)
 Frame = +3

Query: 3   NRLRAQKIEARLSKLMLCGSTLRRVAEVFEKEIEAGLRQQPSSLQMENTYVPELPDGTEE 182
           N    Q +  ++ K++  G +  +   VFE E    +     ++Q   T   E     E 
Sbjct: 21  NMWPGQAMTLKVKKVLYAGKSKYQDVLVFESETYGHVLVLDGAIQA--TERDEF-SYQEM 77

Query: 183 GSFLALDLGGTNFRVLFLELKAGQLLREKVKHYHINDALRLGPGEDLFNFLADCVQDFLV 362
            + LAL+      +VL +    G +LRE VKH  + +A+     ED+       + +   
Sbjct: 78  IAHLALNSHPNPKKVLVIGGGDGGVLREVVKHECVEEAILCDIDEDVIKVSKQYLPEMSA 137

Query: 363 SQSMEKETFSLGFTFSF 413
             +  K    +G  F F
Sbjct: 138 GFNHPKVKVHIGDGFKF 154


>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 574

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = +2

Query: 377 KRNLLFGFHILVSYEAAFDILWRTN 451
           K  L FGF  L  +  +F ++WRTN
Sbjct: 143 KWGLTFGFLTLALFTYSFLMVWRTN 167


>SPAC644.13c |||Rab GTPase binding |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 225

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 12/40 (30%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
 Frame = -2

Query: 195 LGTSLPLFRQ-AILGHKYFPFASSKVVVGVPPRFLSRTPL 79
           LG ++ +F+   ILG+  FP   + +V    P    R P+
Sbjct: 141 LGANISIFQSMCILGYSSFPLMIASIVCAFVPLIFIRIPV 180


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,093,809
Number of Sequences: 5004
Number of extensions: 68268
Number of successful extensions: 211
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)