Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1247
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 187 1e-48
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 187 1e-48
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 187 1e-48
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 74 3e-14
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 34 0.025
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 6.6
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 8.7
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 187 bits (456), Expect = 1e-48
Identities = 84/112 (75%), Positives = 96/112 (85%)
Frame = -1
Query: 651 KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 472
K PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E
Sbjct: 328 KNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEE 387
Query: 471 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 316
+PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 388 SPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
Score = 35.1 bits (77), Expect = 0.011
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = -3
Query: 688 KELRRGYVAGDSKNNPT*GCCRF 620
K++RRG V GDSKN+P GC F
Sbjct: 316 KDIRRGNVCGDSKNDPPMGCASF 338
Score = 28.3 bits (60), Expect = 1.2
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -2
Query: 722 NVGFNVKNVSVQGIASWLCC 663
NVGFNVKNVSV+ I C
Sbjct: 305 NVGFNVKNVSVKDIRRGNVC 324
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 187 bits (456), Expect = 1e-48
Identities = 84/112 (75%), Positives = 96/112 (85%)
Frame = -1
Query: 651 KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 472
K PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E
Sbjct: 328 KNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEE 387
Query: 471 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 316
+PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 388 SPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
Score = 35.1 bits (77), Expect = 0.011
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = -3
Query: 688 KELRRGYVAGDSKNNPT*GCCRF 620
K++RRG V GDSKN+P GC F
Sbjct: 316 KDIRRGNVCGDSKNDPPMGCASF 338
Score = 28.3 bits (60), Expect = 1.2
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -2
Query: 722 NVGFNVKNVSVQGIASWLCC 663
NVGFNVKNVSV+ I C
Sbjct: 305 NVGFNVKNVSVKDIRRGNVC 324
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 187 bits (456), Expect = 1e-48
Identities = 84/112 (75%), Positives = 96/112 (85%)
Frame = -1
Query: 651 KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 472
K PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E
Sbjct: 328 KNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEE 387
Query: 471 NPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 316
+PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 388 SPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
Score = 35.1 bits (77), Expect = 0.011
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = -3
Query: 688 KELRRGYVAGDSKNNPT*GCCRF 620
K++RRG V GDSKN+P GC F
Sbjct: 316 KDIRRGNVCGDSKNDPPMGCASF 338
Score = 28.3 bits (60), Expect = 1.2
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -2
Query: 722 NVGFNVKNVSVQGIASWLCC 663
NVGFNVKNVSV+ I C
Sbjct: 305 NVGFNVKNVSVKDIRRGNVC 324
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 73.7 bits (173), Expect = 3e-14
Identities = 45/145 (31%), Positives = 74/145 (51%), Gaps = 2/145 (1%)
Frame = -1
Query: 741 EAVPGAQCRFQRKERVRPRNC-VVVMLLVTPKTTPPKGAADFTAQVIVLNHPGQISNGYT 565
E + + C Q + RVR + V ++T P F AQ+ +L P ++ GY+
Sbjct: 519 EEISSSICGDQVRLRVRGDDSDVQTGYVLTSTKNPVHATTRFIAQIAILELPSILTTGYS 578
Query: 564 PVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEF 385
V+ HTA FA++ K+D+ T + ++ P G I L P+C+E F+++
Sbjct: 579 CVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDY 637
Query: 384 PPLGRFAVRDMRQTVAVG-VIKAVN 313
+GRF +RD TVAVG V+K ++
Sbjct: 638 QYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 33.9 bits (74), Expect = 0.025
Identities = 31/107 (28%), Positives = 46/107 (42%)
Frame = -1
Query: 642 PPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPK 463
P + F A++ + G I +G T VL H+ + K+ K + +
Sbjct: 491 PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVNNKRSR-HIA 544
Query: 462 SIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 322
S K I L PLC+ +E P LGRF +R TVA G++K
Sbjct: 545 SRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 6.6
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 272 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 445
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 446 SPDLMDFGLTSVDLPVRRSTFSLIS 520
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 437 MAASPDLMDFGLTSVDLPVRRSTFS 511
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,989,105
Number of Sequences: 5004
Number of extensions: 60528
Number of successful extensions: 178
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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