Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1212
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC336.10c |tif512||translation initiation factor|Schizosacchar... 50 5e-07
SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizos... 48 1e-06
SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak... 28 1.2
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 27 2.9
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 27 2.9
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 27 3.8
SPCC569.07 |||aromatic aminotransferase |Schizosaccharomyces pom... 26 5.0
SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 26 5.0
SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyc... 26 6.6
SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa... 26 6.6
SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin Nup132|Schizosa... 25 8.7
>SPBC336.10c |tif512||translation initiation
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 157
Score = 49.6 bits (113), Expect = 5e-07
Identities = 27/71 (38%), Positives = 42/71 (59%)
Frame = -1
Query: 603 PHVKREDYQLTDISDDGYLTLMADNGDLREDLKNPGW*PRAHSCVLTSTSGKELLCTVLK 424
P VKR++YQL +I DDGYL LM +G ++D++ P + GK+L+ TV+
Sbjct: 84 PVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEG-ELGNEIEEGFEEGKDLIITVVS 141
Query: 423 SCGEECVIAVK 391
+ GEE +A +
Sbjct: 142 AMGEEIALACR 152
Score = 39.9 bits (89), Expect = 4e-04
Identities = 15/21 (71%), Positives = 19/21 (90%)
Frame = -2
Query: 686 KVHLVGIDIFNGKKYEDICPS 624
KVH+V +DIFNG+KYED+ PS
Sbjct: 57 KVHIVALDIFNGRKYEDMSPS 77
Score = 35.9 bits (79), Expect = 0.006
Identities = 22/49 (44%), Positives = 25/49 (51%)
Frame = -3
Query: 748 GXPCKIVKMSPSKTGKARPPXKFTWLGLISSMVKSMKISVPPPHNMDVP 602
G PCKIV MS SKTGK K + L + + P HNMDVP
Sbjct: 37 GRPCKIVDMSTSKTGK-HGHAKVHIVALDIFNGRKYEDMSPSTHNMDVP 84
>SPAC26H5.10c |tif51||translation initiation factor
eIF5A|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 48.4 bits (110), Expect = 1e-06
Identities = 26/71 (36%), Positives = 42/71 (59%)
Frame = -1
Query: 603 PHVKREDYQLTDISDDGYLTLMADNGDLREDLKNPGW*PRAHSCVLTSTSGKELLCTVLK 424
P VKR++YQL +I DDGYL LM +G ++D++ P + G++L+ TV+
Sbjct: 84 PVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEG-ELGNEIEEGFDEGRDLIITVVS 141
Query: 423 SCGEECVIAVK 391
+ GEE +A +
Sbjct: 142 AMGEETALACR 152
Score = 39.9 bits (89), Expect = 4e-04
Identities = 15/21 (71%), Positives = 19/21 (90%)
Frame = -2
Query: 686 KVHLVGIDIFNGKKYEDICPS 624
KVH+V +DIFNG+KYED+ PS
Sbjct: 57 KVHIVALDIFNGRKYEDMSPS 77
Score = 35.9 bits (79), Expect = 0.006
Identities = 22/49 (44%), Positives = 25/49 (51%)
Frame = -3
Query: 748 GXPCKIVKMSPSKTGKARPPXKFTWLGLISSMVKSMKISVPPPHNMDVP 602
G PCKIV MS SKTGK K + L + + P HNMDVP
Sbjct: 37 GRPCKIVDMSTSKTGK-HGHAKVHIVALDIFNGRKYEDMSPSTHNMDVP 84
>SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak10
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 708
Score = 28.3 bits (60), Expect = 1.2
Identities = 9/24 (37%), Positives = 17/24 (70%)
Frame = +3
Query: 435 CTTIPCRWSKSVRNCVPEVTIRDF 506
C+ PCR+ +++ N +PE+T+ F
Sbjct: 438 CSHNPCRFRRNLINLLPEITVAHF 461
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 27.1 bits (57), Expect = 2.9
Identities = 15/54 (27%), Positives = 23/54 (42%)
Frame = +1
Query: 370 FVESCVCFDGDDALLTAGFQHGAQQFLAAGRSQYATVCPRSPSGIFEVLTQVTV 531
F+ V F GD +L + A QF + QY +C + I + Q+ V
Sbjct: 257 FLLRIVSFSGDSSLKSFSLHFFALQFFSTSLIQYTHICRKCVITILQSYQQLIV 310
>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
transporting Cta4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1211
Score = 27.1 bits (57), Expect = 2.9
Identities = 10/38 (26%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = -1
Query: 633 LSLXHTTWTYPHVK--REDYQLTDISDDGYLTLMADNG 526
+S+ W Y V ++++ ++ + +GY+TLM +G
Sbjct: 788 MSIFTHAWVYARVSPSQKEFMISTLKHNGYITLMCGDG 825
>SPAC13G7.04c |mac1||membrane anchored protein Mac1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 756
Score = 26.6 bits (56), Expect = 3.8
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -2
Query: 413 RSASSPSKQTQLSTNKPTQH 354
+S SSPS+ + LST+KP H
Sbjct: 286 KSESSPSRSSVLSTSKPEVH 305
>SPCC569.07 |||aromatic aminotransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 470
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +1
Query: 463 SQYATVCPRSPSGIFEVLTQVTVVSHQGQVAIIRDISQL 579
++YA VC SPSG+ +V+ +++ GQ + + L
Sbjct: 315 TRYAEVCTESPSGVSQVVL-YAILNRWGQNGFLEWLQDL 352
>SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 26.2 bits (55), Expect = 5.0
Identities = 16/53 (30%), Positives = 25/53 (47%)
Frame = -1
Query: 438 CTVLKSCGEECVIAVKANTALDK*TNSAFIGLTNF*FFTINNSYINVKHNIVY 280
C V+ E + NT D +N IG FF +N +++NVK ++Y
Sbjct: 219 CVVINKDSEFLQMCRPINTTQD--SNEHSIGSL---FFYLNYNFVNVKRQVIY 266
>SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 835
Score = 25.8 bits (54), Expect = 6.6
Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 5/44 (11%)
Frame = +1
Query: 520 QVTVVSHQGQVAIIRDISQLVVFAL-----HVGVRPCCVXEGQI 636
++ VVS QGQV + + + F+L ++G+R C EG +
Sbjct: 102 ELIVVSKQGQVRVYNLLGEFHQFSLGKGVENIGIRECQFSEGGV 145
>SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase
Ubp7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 875
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -1
Query: 609 TYPHVKREDYQLTDISDDGYL 547
T +KREDY+LT ++ GYL
Sbjct: 87 TTEKIKREDYELTLCANCGYL 107
>SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin
Nup132|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1162
Score = 25.4 bits (53), Expect = 8.7
Identities = 15/59 (25%), Positives = 33/59 (55%)
Frame = +1
Query: 508 EVLTQVTVVSHQGQVAIIRDISQLVVFALHVGVRPCCVXEGQISSYFLPLKISIPTK*T 684
+V+ +V GQV ++ + + F+L+ R + EG+++ Y+L L++ + T+ T
Sbjct: 978 QVMQRVVKKFIAGQVVEATELLEYLSFSLY---RREDLVEGEVTDYYLALRLLLTTRLT 1033
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,663,816
Number of Sequences: 5004
Number of extensions: 50870
Number of successful extensions: 223
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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