Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1176
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 237 1e-63
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 116 4e-27
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 103 2e-23
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 99 5e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 92 4e-20
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 68 1e-12
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 66 3e-12
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 62 6e-11
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 2e-05
SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr 1... 27 2.0
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 6.0
SPAC1B3.17 |clr2||chromatin silencing protein Clr2|Schizosacchar... 25 7.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 237 bits (579), Expect = 1e-63
Identities = 109/132 (82%), Positives = 115/132 (87%)
Frame = -3
Query: 692 PXGQVITIGNERFRCPEXXFPTFVLGMEACGIHETTYNSIMKCDVDIXKDLYANTVLSGG 513
P GQVITIGNERFR PE F LG+E GIHE TYNSIMKCDVDI KDLY N V+SGG
Sbjct: 243 PDGQVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGG 302
Query: 512 TTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLXTFQQMWISKQEY 333
TTMYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASL TFQQMWISKQEY
Sbjct: 303 TTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEY 362
Query: 332 DESGPSIVHRKC 297
DESGP IV+RKC
Sbjct: 363 DESGPGIVYRKC 374
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 116 bits (278), Expect = 4e-27
Identities = 54/131 (41%), Positives = 80/131 (61%)
Frame = -3
Query: 692 PXGQVITIGNERFRCPEXXFPTFVLGMEACGIHETTYNSIMKCDVDIXKDLYANTVLSGG 513
P Q IT+G E F PE F ++G EA G+H + SI+ D+D+ LY+N VLSGG
Sbjct: 248 PDNQTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGG 307
Query: 512 TTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLXTFQQMWISKQEY 333
+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASL TF+++ I+ +EY
Sbjct: 308 STLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY 367
Query: 332 DESGPSIVHRK 300
I R+
Sbjct: 368 KNDQNVIFRRR 378
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 103 bits (247), Expect = 2e-23
Identities = 55/136 (40%), Positives = 83/136 (61%), Gaps = 12/136 (8%)
Frame = -3
Query: 692 PXGQVITIGNERFRCPEXXFPTFVLGMEACGIHETTYNSIMKCDVDIXKDLYANTVLSGG 513
P G+VI +G+ER+ CPE F ++G E G+ E +++I DVDI K LY VLSGG
Sbjct: 243 PDGRVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGG 302
Query: 512 TTMYPGIADRMQKEITAL--------APSTM---KIKIIAPPERKYSVWIGGSILASLXT 366
++MY G+ R++KEI L P+ + K+KI P R+++V+IGG++LA +
Sbjct: 303 SSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMA 362
Query: 365 -FQQMWISKQEYDESG 321
MW+SK E++E G
Sbjct: 363 QNDHMWVSKAEWEEYG 378
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 99.1 bits (236), Expect = 5e-22
Identities = 55/135 (40%), Positives = 74/135 (54%), Gaps = 11/135 (8%)
Frame = -3
Query: 692 PXGQVITIGNERFRCPEXXF-PTFVLGMEA--------CGIHETTYNSIMKCDVDIXKDL 540
P G G ERFR E F P+F A G+HE Y SI+ CD ++ L
Sbjct: 284 PDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPL 343
Query: 539 YANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKII-APPERKYSVWIGGSILASLXT 366
N V++GGT++ PG+++R+Q E+ LA S + + +VW GGSILASL
Sbjct: 344 LNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDN 403
Query: 365 FQQMWISKQEYDESG 321
FQ +W+SKQEYDE G
Sbjct: 404 FQHLWVSKQEYDEVG 418
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 92.3 bits (219), Expect(2) = 4e-20
Identities = 46/108 (42%), Positives = 70/108 (64%), Gaps = 6/108 (5%)
Frame = -3
Query: 602 GIHETTYNSIMKCDVDIXKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 423
GI + N I +CDVDI L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384
Query: 422 P---ERKYSVWIGGSILASLXTFQQMWISKQEYDESGP---SIVHRKC 297
ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C
Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432
Score = 21.0 bits (42), Expect(2) = 4e-20
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = -3
Query: 692 PXGQVITIGNERFRCPEXXF 633
P G + G ER++ PE F
Sbjct: 267 PDGSRMMFGAERYQIPEHLF 286
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 67.7 bits (158), Expect = 1e-12
Identities = 39/128 (30%), Positives = 60/128 (46%)
Frame = -3
Query: 683 QVITIGNERFRCPEXXFPTFVLGMEACGIHETTYNSIMKCDVDIXKDLYANTVLSGGTTM 504
QV+ + NERF PE F + + GI E S+ +I L N V GG
Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCK 331
Query: 503 YPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLXTFQQMWISKQEYDES 324
+PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E
Sbjct: 332 FPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEH 390
Query: 323 GPSIVHRK 300
G +I+ RK
Sbjct: 391 GANIMTRK 398
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 66.5 bits (155), Expect = 3e-12
Identities = 40/140 (28%), Positives = 65/140 (46%), Gaps = 15/140 (10%)
Frame = -3
Query: 677 ITIGNERFRCPEXXF-PTFVLGMEACGIHETTYNSIMKCDVDIXKDLYANTVLSGGTTMY 501
I +G ERF PE F P + E N + +D+ K LY N VLSGG+T++
Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLF 339
Query: 500 PGIADRMQKEITALAPST--------------MKIKIIAPPERKYSVWIGGSILASLXTF 363
+R+Q+++ + + + +I+ ++ +VW GGS+LA F
Sbjct: 340 KNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEF 399
Query: 362 QQMWISKQEYDESGPSIVHR 303
+K +Y+E G SI R
Sbjct: 400 GSYCHTKADYEEYGASIARR 419
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 62.5 bits (145), Expect = 6e-11
Identities = 29/118 (24%), Positives = 59/118 (50%)
Frame = -3
Query: 662 ERFRCPEXXFPTFVLGMEACGIHETTYNSIMKCDVDIXKDLYANTVLSGGTTMYPGIADR 483
E+ R PE F ++G++ GI E + + + ++ + L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 482 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLXTFQQMWISKQEYDESGPSIV 309
+++E+T++ P I + W G S + F+ ++++EY E GP +
Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGPEYI 711
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 44.4 bits (100), Expect = 2e-05
Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
Frame = -3
Query: 548 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 378
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 377 SLXTFQQMWISKQEYDESGPSIVHRK 300
+ ++WI ++ G ++ K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612
>SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 455
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +1
Query: 223 NGDYKPELSSRPRAAGGSDARCVXKHFLCTMEGPDSSYSC 342
NG Y +++ P+ AG +C H L ME + +SC
Sbjct: 225 NGAYVEQMNQIPKLAG----KCTGDHMLINMEWGATDFSC 260
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 25.8 bits (54), Expect = 6.0
Identities = 11/37 (29%), Positives = 23/37 (62%), Gaps = 3/37 (8%)
Frame = -3
Query: 401 WIGGSILASLXTFQQM---WISKQEYDESGPSIVHRK 300
++GGSI+A + + +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPAC1B3.17 |clr2||chromatin silencing protein
Clr2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 537
Score = 25.4 bits (53), Expect = 7.9
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = -2
Query: 519 RWYHHVPWNRRPYAKGNHSSRPIDNED*DHCSSREEVLRMDRWIDPRLPLYLPTDVDLET 340
RWY PW + KG+ + +E + SSR + + W+D YL +++D+ +
Sbjct: 483 RWYE--PW----FVKGDLN---YTSEVKERTSSRLSAVGSENWVDDDFYEYLLSEIDMVS 533
Query: 339 GV 334
V
Sbjct: 534 AV 535
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,472,787
Number of Sequences: 5004
Number of extensions: 45940
Number of successful extensions: 105
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 96
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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