Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1142
(600 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 83 3e-17
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 54 2e-08
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi... 48 8e-07
SPBC215.11c |||aldo/keto reductase, unknown biological role|Schi... 41 1e-04
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 38 8e-04
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 37 0.002
SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|c... 29 0.39
SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|c... 27 2.1
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 26 3.7
SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|c... 26 4.8
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 26 4.8
SPBC12C2.04 |||NAD binding dehydrogenase family protein|Schizosa... 25 6.4
SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe... 25 6.4
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 25 6.4
SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|... 25 6.4
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 25 8.5
SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual 25 8.5
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 83.0 bits (196), Expect = 3e-17
Identities = 58/167 (34%), Positives = 88/167 (52%), Gaps = 4/167 (2%)
Frame = +1
Query: 16 QSFNMRYNELGTTGIKVSHISVGSAAFSNIYGKYDEKRSLELIKESLNLGINYLETGPWY 195
Q N+ + LG +G+KVS S+G G YD + + +K++ +LGIN +T Y
Sbjct: 9 QPKNVPFRFLGRSGLKVSAFSLGGWLTYGNEG-YDVEHTKNCLKQAWDLGINTFDTAEIY 67
Query: 196 GQGSSEKVIGKAL--VGVPRDSYYIGSKV--GRYDKDTLKMFDFSAEKTAAGLDNTLSLL 363
G+SE V+GKA+ +G R Y I +KV G K S + GL+ +L L
Sbjct: 68 SNGNSETVMGKAIKELGWDRSEYVITTKVFFGAGTK-LPNTTGLSRKHIIEGLNASLKRL 126
Query: 364 GLDYVDLIQVHDITFAPDTSVVLKETLPVLEQAVRDGKARFIGIADY 504
GL YVD+I H PD SV ++E + Q ++DGKA + G +++
Sbjct: 127 GLPYVDVIMAH----RPDPSVPMEEVVRAFTQLIQDGKAFYWGTSEW 169
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 54.0 bits (124), Expect = 2e-08
Identities = 39/150 (26%), Positives = 77/150 (51%), Gaps = 4/150 (2%)
Frame = +1
Query: 64 VSHISVGSAAFSNIYGKYDEKRSLELIKESLNLGINYLETGPWYGQGSSEKVIGKALVGV 243
V I G +YG E+ + ++ + +LG + ++ YG G++E+ IG+
Sbjct: 13 VPAIGFGCMGLHAMYGPSSEEANQAVLTHAADLGCTFWDSSDMYGFGANEECIGRWFKQT 72
Query: 244 -PRDSYYIGSKVGRYDK--DTLKM-FDFSAEKTAAGLDNTLSLLGLDYVDLIQVHDITFA 411
R ++ +K G Y+K +T ++ + + LD +L LG+D +DL VH F+
Sbjct: 73 GRRKEIFLATKFG-YEKNPETGELSLNNEPDYIEKALDLSLKRLGIDCIDLYYVH--RFS 129
Query: 412 PDTSVVLKETLPVLEQAVRDGKARFIGIAD 501
+T + ++ + L++ V GK R+IG+++
Sbjct: 130 GETPI--EKIMGALKKCVEAGKIRYIGLSE 157
>SPAC977.14c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 351
Score = 48.4 bits (110), Expect = 8e-07
Identities = 50/181 (27%), Positives = 83/181 (45%), Gaps = 28/181 (15%)
Frame = +1
Query: 34 YNELGTTGIKVSHISVGSAAFSNI-----YGKYDEKRSLELIKESLNLGINYLETGPWYG 198
Y LG +G+KVS + +G ++ + DE+ +++K + + GI +T Y
Sbjct: 9 YGCLGNSGLKVSKLILGCMSYGKKEYWEDWVLEDEEEVFKIMKAAYDAGIRTFDTANCYS 68
Query: 199 QGSSEKVIGKAL--VGVPRDSYYIGSK-VGRYDKDTLKMF-DFSAE----------KTAA 336
G SE+++GK + +PR S I SK KD +K+F D S+
Sbjct: 69 AGVSEELVGKFIRKYEIPRSSIVILSKCFFPVRKDLIKIFGDLSSRGVHFLDSPELANQC 128
Query: 337 GL---------DNTLSLLGLDYVDLIQVHDITFAPDTSVVLKETLPVLEQAVRDGKARFI 489
GL ++++ LG Y+D++Q+H D V +E + L V GK R+I
Sbjct: 129 GLSRKHIFDAVEDSVKRLG-TYIDVLQIHRY----DPHVSAEEVMRALNDVVESGKVRYI 183
Query: 490 G 492
G
Sbjct: 184 G 184
>SPBC215.11c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 41.1 bits (92), Expect = 1e-04
Identities = 34/137 (24%), Positives = 62/137 (45%), Gaps = 1/137 (0%)
Frame = +1
Query: 118 DEKRSLELIKESLNLGINYLETGPWYGQGSSEKVIGKALVGVPRDSYYIGSKVGRYDKDT 297
D++ + +K L IN+++T YG SE ++ +AL P I +K G
Sbjct: 46 DKEACIATLKRLPELNINFIDTADSYGPEVSENLLREAL--YPYKGLIIATKGGLVRTGP 103
Query: 298 LKMFDFSAEK-TAAGLDNTLSLLGLDYVDLIQVHDITFAPDTSVVLKETLPVLEQAVRDG 474
+ A K + ++ LG+ +DL Q+H I D V K+ + ++G
Sbjct: 104 NEWHPCGAPKFLRQEVLMSMRRLGVKQIDLWQLHRI----DPKVPRKDQFSEIAAMKKEG 159
Query: 475 KARFIGIADYDIDLMKE 525
R +G+++ +D +KE
Sbjct: 160 LIRHVGLSEVTVDDIKE 176
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 38.3 bits (85), Expect = 8e-04
Identities = 38/147 (25%), Positives = 74/147 (50%), Gaps = 4/147 (2%)
Frame = +1
Query: 106 YGKYDEKRS--LELIKESLNLGINYLETGPWYGQGSSEKVIGKALVGVPRDSYYIGSKVG 279
YG Y R+ + + +L G ++++ Y +E G+A++ ++ G+K
Sbjct: 19 YGSYMVNRTKCFDSVYAALQCGYRHIDSAQMY---HNEADCGRAILKFMEET---GTK-- 70
Query: 280 RYDK-DTLKMFDFSAEK-TAAGLDNTLSLLGLDYVDLIQVHDITFAPDTSVVLKETLPVL 453
R D T K+ D S K T + +D ++ GL Y+DL +H +P + E+ L
Sbjct: 71 REDIWFTSKLNDLSGYKSTLSSIDASVKACGLGYIDLFLLH----SPYGDRI--ESWKAL 124
Query: 454 EQAVRDGKARFIGIADYDIDLMKEVVE 534
E+ V +GK R IG++++ ++E+++
Sbjct: 125 EKGVEEGKLRAIGVSNFGPHHIQELLD 151
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 37.1 bits (82), Expect = 0.002
Identities = 34/146 (23%), Positives = 72/146 (49%), Gaps = 4/146 (2%)
Frame = +1
Query: 106 YGKYDEKRSLELIKESLNLGINYLETGPWY-GQGSSEKVIGKAL---VGVPRDSYYIGSK 273
+G Y K + + E+L+LGI +++ Y + E+ I V + R+ + +K
Sbjct: 18 FGVYQLKDCYQQVIEALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKREDITLITK 77
Query: 274 VGRYDKDTLKMFDFSAEKTAAGLDNTLSLLGLDYVDLIQVHDITFAPDTSVVLKETLPVL 453
+ D+L+ F E+T ++ +L G +D++ +H +P V E+ L
Sbjct: 78 I----PDSLQGF----ERTWKAVEQSLRRTGRPKLDVVLIH----SPKWPVRRIESWRAL 125
Query: 454 EQAVRDGKARFIGIADYDIDLMKEVV 531
Q ++G+ IG+++Y+I ++E++
Sbjct: 126 LQHQKEGRINKIGVSNYNIHHLEEII 151
>SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 334
Score = 29.5 bits (63), Expect = 0.39
Identities = 32/158 (20%), Positives = 72/158 (45%), Gaps = 5/158 (3%)
Frame = +1
Query: 64 VSHISVGSAAFSNIYGKYDEKRSLELIKESLNLGINYLETGPWYGQG---SSEKVIGKAL 234
V I +G + + ++ + ++ +L+ G ++ + G +YG ++ +++ +
Sbjct: 9 VGPIGLGLKSLTWTENPVPDEEAFRIMNYALSHGCSFWDAGEFYGLSEPLANLQLLSRYF 68
Query: 235 VGVPR--DSYYIGSKVGRYDKDTLKMFDFSAEKTAAGLDNTLSLLGLDYVDLIQVHDITF 408
P D ++ K G +D +T ++ T + +L + +DL Q I
Sbjct: 69 QKFPDSIDKVFLSVK-GAFDPETHRVHGTRECITKSIKTVRETLKKVKTIDLYQCAAID- 126
Query: 409 APDTSVVLKETLPVLEQAVRDGKARFIGIADYDIDLMK 522
PDT + +ET+ L++ V G R IG+ + ++ +K
Sbjct: 127 -PDTPI--EETMACLKEFVDSGDIRCIGLCEPSVEEIK 161
>SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 27.1 bits (57), Expect = 2.1
Identities = 25/107 (23%), Positives = 45/107 (42%)
Frame = +1
Query: 217 VIGKALVGVPRDSYYIGSKVGRYDKDTLKMFDFSAEKTAAGLDNTLSLLGLDYVDLIQVH 396
VI K + S G V Y K+ + + T+ G+ L +D + I+
Sbjct: 608 VIFKRTSWLKAASVVSGRFVNGYKKNDW-ILGYLFRATSGGIGRVAGLRQIDCIPGIENI 666
Query: 397 DITFAPDTSVVLKETLPVLEQAVRDGKARFIGIADYDIDLMKEVVEE 537
D+T + +E++P+L AV + D ++DL+ E + E
Sbjct: 667 DVTNLVSGHLAYRESMPILLAAVG------FEVLDEEVDLVSEPIPE 707
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 26.2 bits (55), Expect = 3.7
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = -1
Query: 228 LPNDLFGAALTVPGSGFQIV 169
LPN FGAA PGSG V
Sbjct: 119 LPNVFFGAAAAQPGSGTPFV 138
>SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 819
Score = 25.8 bits (54), Expect = 4.8
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = -2
Query: 413 GAKVISCTWIRST*SKPSSERVLSRPAAVFSAEKSNIFRVSLS*RPTLL 267
G + C W +S PSS+ +LS+P + S+E+ + LS TLL
Sbjct: 768 GRVIKICDWYQS----PSSDVLLSKPLQLNSSEEMEQQCIDLSRHNTLL 812
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 25.8 bits (54), Expect = 4.8
Identities = 27/81 (33%), Positives = 38/81 (46%), Gaps = 3/81 (3%)
Frame = +1
Query: 85 SAAFSNIYGKYDEKRSLELIKESLN-LGINYLETGPWYGQGS-SEKVIGKALVGVPR-DS 255
SA S I K DEK LEL+ L LG+ + GQ + +++ + V R
Sbjct: 212 SAEGSPILDKRDEKTRLELLSYILKLLGLKVPKDTSKCGQPTLTDQYLFPNNVETKRFIE 271
Query: 256 YYIGSKVGRYDKDTLKMFDFS 318
+ +KV D+DTL F FS
Sbjct: 272 KALTNKVKNQDEDTLYTFQFS 292
>SPBC12C2.04 |||NAD binding dehydrogenase family
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 25.4 bits (53), Expect = 6.4
Identities = 12/25 (48%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
Frame = +1
Query: 163 GINY-LETGPWYGQGSSEKVIGKAL 234
GIN+ GPW EKV+GK+L
Sbjct: 23 GINFGTPEGPWNNAQRVEKVLGKSL 47
>SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 527
Score = 25.4 bits (53), Expect = 6.4
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = -1
Query: 546 TSGLLYYFLHQVDIIVSDPNEASFSISHGLLQHWQRLFEYNXSVR 412
+S +L +FL+Q+ I ++ P+ +FS H + LF SVR
Sbjct: 24 SSRILTFFLNQLTIRLTSPSAYAFSSIHFEILQSTILFLSRESVR 68
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 25.4 bits (53), Expect = 6.4
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -2
Query: 416 SGAKVISCTWIRST*SKPSSERVLSRPAAVFS 321
+G++ IS +W+ T S+P + VLS + FS
Sbjct: 1529 AGSEDISSSWLGRTVSRPRLDTVLSSLGSKFS 1560
>SPAC3A12.05c |taf2||TATA-binding protein associated factor
Taf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1174
Score = 25.4 bits (53), Expect = 6.4
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -3
Query: 595 LXKSVDFXIREYGXY 551
L K++DF +REYG Y
Sbjct: 324 LYKAMDFFVREYGSY 338
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 25.0 bits (52), Expect = 8.5
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +3
Query: 129 KPGAH*RKLESRYQLSGNRTLVRSGQLRKG 218
KP R++ RY L+G ++ GQL G
Sbjct: 687 KPEGEMRRIVGRYGLTGKSQVIPMGQLSDG 716
>SPBC25B2.10 |||Usp |Schizosaccharomyces pombe|chr 2|||Manual
Length = 307
Score = 25.0 bits (52), Expect = 8.5
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +2
Query: 101 ISMGNMTRKEAWSSLKKA*ISVSTIWKPDPGTVRAAPKRSLGRL*LEFPVIR 256
+S GN+ R E +S + +S+ST K + P RS R +E P R
Sbjct: 13 VSFGNLPRPEPRTSKDQQKLSISTDVKKPSHSAPVTPVRS-ARSSMEQPTFR 63
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.315 0.135 0.377
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,373,460
Number of Sequences: 5004
Number of extensions: 47608
Number of successful extensions: 104
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
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