Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-1088
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 83 4e-17
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi... 57 3e-09
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 54 2e-08
SPBC215.11c |||aldo/keto reductase, unknown biological role|Schi... 41 1e-04
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 38 0.001
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 37 0.002
SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|c... 35 0.013
SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|c... 27 2.6
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 26 4.5
SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces pombe... 26 6.0
SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|c... 26 6.0
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 26 6.0
SPBC12C2.04 |||NAD binding dehydrogenase family protein|Schizosa... 25 7.9
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 25 7.9
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 83.0 bits (196), Expect = 4e-17
Identities = 58/167 (34%), Positives = 88/167 (52%), Gaps = 4/167 (2%)
Frame = +2
Query: 11 QSFNMRYNELGTTGIKVSHISVGSAAFSNIYGKYDEKRSLELIKESLNLGINYLETGPWY 190
Q N+ + LG +G+KVS S+G G YD + + +K++ +LGIN +T Y
Sbjct: 9 QPKNVPFRFLGRSGLKVSAFSLGGWLTYGNEG-YDVEHTKNCLKQAWDLGINTFDTAEIY 67
Query: 191 GQGSSEKVIGKAL--VGVPRDSYYIGSKV--GRYDKDTLKMFDFSAEKTAAGLDNTLSLL 358
G+SE V+GKA+ +G R Y I +KV G K S + GL+ +L L
Sbjct: 68 SNGNSETVMGKAIKELGWDRSEYVITTKVFFGAGTK-LPNTTGLSRKHIIEGLNASLKRL 126
Query: 359 GLDYVDLIQVHDITFAPDTSVVLKETLPVLEQAVRDGKARFIGIADY 499
GL YVD+I H PD SV ++E + Q ++DGKA + G +++
Sbjct: 127 GLPYVDVIMAH----RPDPSVPMEEVVRAFTQLIQDGKAFYWGTSEW 169
>SPAC977.14c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 351
Score = 56.8 bits (131), Expect = 3e-09
Identities = 68/252 (26%), Positives = 109/252 (43%), Gaps = 36/252 (14%)
Frame = +2
Query: 29 YNELGTTGIKVSHISVGSAAFSNI-----YGKYDEKRSLELIKESLNLGINYLETGPWYG 193
Y LG +G+KVS + +G ++ + DE+ +++K + + GI +T Y
Sbjct: 9 YGCLGNSGLKVSKLILGCMSYGKKEYWEDWVLEDEEEVFKIMKAAYDAGIRTFDTANCYS 68
Query: 194 QGSSEKVIGKAL--VGVPRDSYYIGSK-VGRYDKDTLKMF-DFSAE----------KTAA 331
G SE+++GK + +PR S I SK KD +K+F D S+
Sbjct: 69 AGVSEELVGKFIRKYEIPRSSIVILSKCFFPVRKDLIKIFGDLSSRGVHFLDSPELANQC 128
Query: 332 GL---------DNTLSLLGLDYVDLIQVHDITFAPDTSVVLKETLPVLEQAVRDGKARFI 484
GL ++++ LG Y+D++Q+H D V +E + L V GK R+I
Sbjct: 129 GLSRKHIFDAVEDSVKRLG-TYIDVLQIHRY----DPHVSAEEVMRALNDVVESGKVRYI 183
Query: 485 GIAD---YDIDLMKEVVEESG-----SQDINHTLLFRSPRSSIIAYRTILASSXSKGVGV 640
G + Y ++ E+ G S H LL+R +I Y GVG+
Sbjct: 184 GASTMRCYQFIELQNTAEKHGWHKFISMQNYHNLLYREEEREMIPY------CQKTGVGL 237
Query: 641 INAAATGMGLLT 676
I + GLLT
Sbjct: 238 IPWSPLARGLLT 249
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 54.0 bits (124), Expect = 2e-08
Identities = 39/150 (26%), Positives = 77/150 (51%), Gaps = 4/150 (2%)
Frame = +2
Query: 59 VSHISVGSAAFSNIYGKYDEKRSLELIKESLNLGINYLETGPWYGQGSSEKVIGKALVGV 238
V I G +YG E+ + ++ + +LG + ++ YG G++E+ IG+
Sbjct: 13 VPAIGFGCMGLHAMYGPSSEEANQAVLTHAADLGCTFWDSSDMYGFGANEECIGRWFKQT 72
Query: 239 -PRDSYYIGSKVGRYDK--DTLKM-FDFSAEKTAAGLDNTLSLLGLDYVDLIQVHDITFA 406
R ++ +K G Y+K +T ++ + + LD +L LG+D +DL VH F+
Sbjct: 73 GRRKEIFLATKFG-YEKNPETGELSLNNEPDYIEKALDLSLKRLGIDCIDLYYVH--RFS 129
Query: 407 PDTSVVLKETLPVLEQAVRDGKARFIGIAD 496
+T + ++ + L++ V GK R+IG+++
Sbjct: 130 GETPI--EKIMGALKKCVEAGKIRYIGLSE 157
>SPBC215.11c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 41.1 bits (92), Expect = 1e-04
Identities = 34/137 (24%), Positives = 62/137 (45%), Gaps = 1/137 (0%)
Frame = +2
Query: 113 DEKRSLELIKESLNLGINYLETGPWYGQGSSEKVIGKALVGVPRDSYYIGSKVGRYDKDT 292
D++ + +K L IN+++T YG SE ++ +AL P I +K G
Sbjct: 46 DKEACIATLKRLPELNINFIDTADSYGPEVSENLLREAL--YPYKGLIIATKGGLVRTGP 103
Query: 293 LKMFDFSAEK-TAAGLDNTLSLLGLDYVDLIQVHDITFAPDTSVVLKETLPVLEQAVRDG 469
+ A K + ++ LG+ +DL Q+H I D V K+ + ++G
Sbjct: 104 NEWHPCGAPKFLRQEVLMSMRRLGVKQIDLWQLHRI----DPKVPRKDQFSEIAAMKKEG 159
Query: 470 KARFIGIADYDIDLMKE 520
R +G+++ +D +KE
Sbjct: 160 LIRHVGLSEVTVDDIKE 176
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 38.3 bits (85), Expect = 0.001
Identities = 38/147 (25%), Positives = 74/147 (50%), Gaps = 4/147 (2%)
Frame = +2
Query: 101 YGKYDEKRS--LELIKESLNLGINYLETGPWYGQGSSEKVIGKALVGVPRDSYYIGSKVG 274
YG Y R+ + + +L G ++++ Y +E G+A++ ++ G+K
Sbjct: 19 YGSYMVNRTKCFDSVYAALQCGYRHIDSAQMY---HNEADCGRAILKFMEET---GTK-- 70
Query: 275 RYDK-DTLKMFDFSAEK-TAAGLDNTLSLLGLDYVDLIQVHDITFAPDTSVVLKETLPVL 448
R D T K+ D S K T + +D ++ GL Y+DL +H +P + E+ L
Sbjct: 71 REDIWFTSKLNDLSGYKSTLSSIDASVKACGLGYIDLFLLH----SPYGDRI--ESWKAL 124
Query: 449 EQAVRDGKARFIGIADYDIDLMKEVVE 529
E+ V +GK R IG++++ ++E+++
Sbjct: 125 EKGVEEGKLRAIGVSNFGPHHIQELLD 151
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 37.1 bits (82), Expect = 0.002
Identities = 34/146 (23%), Positives = 72/146 (49%), Gaps = 4/146 (2%)
Frame = +2
Query: 101 YGKYDEKRSLELIKESLNLGINYLETGPWY-GQGSSEKVIGKAL---VGVPRDSYYIGSK 268
+G Y K + + E+L+LGI +++ Y + E+ I V + R+ + +K
Sbjct: 18 FGVYQLKDCYQQVIEALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKREDITLITK 77
Query: 269 VGRYDKDTLKMFDFSAEKTAAGLDNTLSLLGLDYVDLIQVHDITFAPDTSVVLKETLPVL 448
+ D+L+ F E+T ++ +L G +D++ +H +P V E+ L
Sbjct: 78 I----PDSLQGF----ERTWKAVEQSLRRTGRPKLDVVLIH----SPKWPVRRIESWRAL 125
Query: 449 EQAVRDGKARFIGIADYDIDLMKEVV 526
Q ++G+ IG+++Y+I ++E++
Sbjct: 126 LQHQKEGRINKIGVSNYNIHHLEEII 151
>SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 334
Score = 34.7 bits (76), Expect = 0.013
Identities = 42/215 (19%), Positives = 95/215 (44%), Gaps = 7/215 (3%)
Frame = +2
Query: 59 VSHISVGSAAFSNIYGKYDEKRSLELIKESLNLGINYLETGPWYGQG---SSEKVIGKAL 229
V I +G + + ++ + ++ +L+ G ++ + G +YG ++ +++ +
Sbjct: 9 VGPIGLGLKSLTWTENPVPDEEAFRIMNYALSHGCSFWDAGEFYGLSEPLANLQLLSRYF 68
Query: 230 VGVPR--DSYYIGSKVGRYDKDTLKMFDFSAEKTAAGLDNTLSLLGLDYVDLIQVHDITF 403
P D ++ K G +D +T ++ T + +L + +DL Q I
Sbjct: 69 QKFPDSIDKVFLSVK-GAFDPETHRVHGTRECITKSIKTVRETLKKVKTIDLYQCAAID- 126
Query: 404 APDTSVVLKETLPVLEQAVRDGKARFIGIADYDIDLMKEV--VEESGSQDINHTLLFRSP 577
PDT + +ET+ L++ V G R IG+ + ++ +K V + ++++++LFR
Sbjct: 127 -PDTPI--EETMACLKEFVDSGDIRCIGLCEPSVEEIKRAHSVVRIAAIEVHYSMLFRE- 182
Query: 578 RSSIIAYRTILASSXSKGVGVINAAATGMGLLTNK 682
I Y + + ++ + GLLT +
Sbjct: 183 ----IEYNGVKKLCHDLSIPLVAHSPLAHGLLTGR 213
>SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 27.1 bits (57), Expect = 2.6
Identities = 25/107 (23%), Positives = 45/107 (42%)
Frame = +2
Query: 212 VIGKALVGVPRDSYYIGSKVGRYDKDTLKMFDFSAEKTAAGLDNTLSLLGLDYVDLIQVH 391
VI K + S G V Y K+ + + T+ G+ L +D + I+
Sbjct: 608 VIFKRTSWLKAASVVSGRFVNGYKKNDW-ILGYLFRATSGGIGRVAGLRQIDCIPGIENI 666
Query: 392 DITFAPDTSVVLKETLPVLEQAVRDGKARFIGIADYDIDLMKEVVEE 532
D+T + +E++P+L AV + D ++DL+ E + E
Sbjct: 667 DVTNLVSGHLAYRESMPILLAAVG------FEVLDEEVDLVSEPIPE 707
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 26.2 bits (55), Expect = 4.5
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = -1
Query: 223 LPNDLFGAALTVPGSGFQIV 164
LPN FGAA PGSG V
Sbjct: 119 LPNVFFGAAAAQPGSGTPFV 138
>SPBC887.19 |rft1||human RFT1 ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 527
Score = 25.8 bits (54), Expect = 6.0
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = -1
Query: 541 TSGLLYYFLHQVDIIVSDPNEASFSISHGLLQHWQRLFEYNRSVR 407
+S +L +FL+Q+ I ++ P+ +FS H + LF SVR
Sbjct: 24 SSRILTFFLNQLTIRLTSPSAYAFSSIHFEILQSTILFLSRESVR 68
>SPBC530.11c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 819
Score = 25.8 bits (54), Expect = 6.0
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = -2
Query: 408 GAKVISCTWIRST*SKPSSERVLSRPAAVFSAEKSNIFRVSLS*RPTLL 262
G + C W +S PSS+ +LS+P + S+E+ + LS TLL
Sbjct: 768 GRVIKICDWYQS----PSSDVLLSKPLQLNSSEEMEQQCIDLSRHNTLL 812
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 25.8 bits (54), Expect = 6.0
Identities = 27/81 (33%), Positives = 38/81 (46%), Gaps = 3/81 (3%)
Frame = +2
Query: 80 SAAFSNIYGKYDEKRSLELIKESLN-LGINYLETGPWYGQGS-SEKVIGKALVGVPR-DS 250
SA S I K DEK LEL+ L LG+ + GQ + +++ + V R
Sbjct: 212 SAEGSPILDKRDEKTRLELLSYILKLLGLKVPKDTSKCGQPTLTDQYLFPNNVETKRFIE 271
Query: 251 YYIGSKVGRYDKDTLKMFDFS 313
+ +KV D+DTL F FS
Sbjct: 272 KALTNKVKNQDEDTLYTFQFS 292
>SPBC12C2.04 |||NAD binding dehydrogenase family
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 25.4 bits (53), Expect = 7.9
Identities = 12/25 (48%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
Frame = +2
Query: 158 GINY-LETGPWYGQGSSEKVIGKAL 229
GIN+ GPW EKV+GK+L
Sbjct: 23 GINFGTPEGPWNNAQRVEKVLGKSL 47
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 25.4 bits (53), Expect = 7.9
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -2
Query: 411 SGAKVISCTWIRST*SKPSSERVLSRPAAVFS 316
+G++ IS +W+ T S+P + VLS + FS
Sbjct: 1529 AGSEDISSSWLGRTVSRPRLDTVLSSLGSKFS 1560
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,837,142
Number of Sequences: 5004
Number of extensions: 56803
Number of successful extensions: 161
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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