Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-1048
         (750 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase, ...   109   2e-24
At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase f...    44   8e-05
At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase f...    41   8e-04
At1g65010.1 68414.m07368 expressed protein similar to endosome-a...    34   0.12 
At2g19910.1 68415.m02327 RNA-dependent RNA polymerase family pro...    28   7.6  

>At4g17510.1 68417.m02620 ubiquitin carboxyl-terminal hydrolase,
           putative / ubiquitin thiolesterase, putative similar to
           SP|Q9JKB1 Ubiquitin carboxyl-terminal hydrolase isozyme
           L3 (EC 3.4.19.12) (UCH- L3) (Ubiquitin thiolesterase L3)
           {Mus musculus}; contains Pfam profile PF01088: Ubiquitin
           carboxyl-terminal hydrolase, family 1
          Length = 234

 Score =  109 bits (262), Expect = 2e-24
 Identities = 56/133 (42%), Positives = 82/133 (61%), Gaps = 2/133 (1%)
 Frame = +3

Query: 222 VPLESNPDVLNKFLQKLGV-PNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENH 398
           +PLESNPDV+N++L  LG+ P++    DV GLD E L  VP+PVL+V+ L+PI+   E  
Sbjct: 14  LPLESNPDVMNQYLWGLGLAPDEAECNDVYGLDDELLEMVPKPVLAVLFLYPITKKSEEE 73

Query: 399 KKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDG-HMQKFLNE 575
           +  ++ EI  K    S  +++MKQ + NACGTI L+H++ N T  I+LSDG  + +F   
Sbjct: 74  RIEQDKEIKEKVH--SDKVYFMKQTVGNACGTIGLLHAIGNITSEIKLSDGSFLDRFFKS 131

Query: 576 AKGLDATARGKLL 614
              +    R K L
Sbjct: 132 TANMTPMERAKFL 144


>At5g16310.1 68418.m01907 ubiquitin carboxyl-terminal hydrolase
           family 1 protein similar to 26S proteasome regulatory
           complex subunit p37A [Drosophila melanogaster]
           GI:6434962; contains Pfam profile PF01088: Ubiquitin
           carboxyl-terminal hydrolase, family 1
          Length = 334

 Score = 44.4 bits (100), Expect = 8e-05
 Identities = 37/127 (29%), Positives = 68/127 (53%), Gaps = 5/127 (3%)
 Frame = +3

Query: 222 VPLESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHK 401
           +P+ES+P +  + +Q++ V     + ++  LD  +L  + RPV  ++LL      Y+   
Sbjct: 4   LPVESDPGIFTEIIQQMQVKGV-QVEELYSLDFNSLDEI-RPVYGLILL------YKWRP 55

Query: 402 KTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDI---IELSD--GHMQKF 566
           + +EN ++    E + N F+  Q I+NAC T A++  + N++ I    ELS+     ++F
Sbjct: 56  EEKENRVVIT--EPNPNFFFASQIINNACATQAILSVLMNSSSIDIGSELSELKQFAKEF 113

Query: 567 LNEAKGL 587
             E KGL
Sbjct: 114 PPELKGL 120


>At1g65650.1 68414.m07448 ubiquitin carboxyl-terminal hydrolase
           family 1 protein similar to 26S proteasome regulatory
           complex subunit p37A [Drosophila melanogaster]
           GI:6434962; contains Pfam profile PF01088: Ubiquitin
           carboxyl-terminal hydrolase, family 1
          Length = 330

 Score = 41.1 bits (92), Expect = 8e-04
 Identities = 25/102 (24%), Positives = 56/102 (54%)
 Frame = +3

Query: 228 LESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKT 407
           +ES+P V  + +Q++ V     + ++  LD ++L+ + RPV  ++ LF      ++ + T
Sbjct: 6   IESDPGVFTELIQQMQVKGV-QVEELYSLDSDSLNNL-RPVYGLIFLFKWQAGEKDERPT 63

Query: 408 EENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDI 533
            ++++         N+F+  Q I+NAC T A++  + N+ ++
Sbjct: 64  IQDQV--------SNLFFANQVINNACATQAILAILLNSPEV 97


>At1g65010.1 68414.m07368 expressed protein similar to
           endosome-associated protein (GI:1016368) [Homo sapiens];
           similar to Centromeric protein E (CENP-E protein)
           (Swiss-Prot:Q02224) [Homo sapiens]
          Length = 1318

 Score = 33.9 bits (74), Expect = 0.12
 Identities = 24/74 (32%), Positives = 37/74 (50%)
 Frame = +3

Query: 399 KKTEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEA 578
           KK+EE E  S  +EVS  +  +K++  +AC       S+ NN  + E    ++Q+ L EA
Sbjct: 522 KKSEE-ENSSSQEEVSRLVNLLKESEEDACARKEEEASLKNNLKVAEGEVKYLQETLGEA 580

Query: 579 KGLDATARGKLLGK 620
           K      +  LL K
Sbjct: 581 KAESMKLKESLLDK 594


>At2g19910.1 68415.m02327 RNA-dependent RNA polymerase family
           protein contains Pfam domain, PF05183: RNA dependent RNA
           polymerase
          Length = 992

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 13/55 (23%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
 Frame = -3

Query: 370 KSSITESTGRGTHESVSGSSPMTSTIFHLFGTPSFCKNLFRT-SGLDSRGTKVSV 209
           K  + E+TG   H+ +   + +T     + G  ++C +L+ T  G+   G  V +
Sbjct: 229 KGHLLENTGTHLHKVLGDDNVLTVKFDKVLGVETYCNDLYSTYKGIAKNGIMVGL 283


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,021,987
Number of Sequences: 28952
Number of extensions: 328768
Number of successful extensions: 739
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 723
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 736
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)