BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-1034 (650 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 221 5e-59 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 99 3e-22 SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 66 5e-12 SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 59 5e-10 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 59 5e-10 SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 49 7e-07 SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosac... 33 0.047 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 29 0.77 SPBC336.08 |spc24||spindle pole body protein Spc24|Schizosacchar... 29 0.77 SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosacchar... 28 1.0 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 28 1.3 SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 1.3 SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 28 1.3 SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|c... 27 1.8 SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 27 1.8 SPBC32H8.09 |||WD repeat protein, human WDR8 family|Schizosaccha... 27 1.8 SPAC30D11.08c |phf2|swp2, saf60|PHD finger containing protein Ph... 27 3.1 SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 27 3.1 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 26 4.1 SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha sub... 26 5.4 SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pomb... 25 7.2 SPCC126.14 |prp18||U5 snRNP-associated protein Prp18|Schizosacch... 25 9.5 SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 25 9.5 SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 25 9.5 >SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizosaccharomyces pombe|chr 3|||Manual Length = 389 Score = 221 bits (541), Expect = 5e-59 Identities = 103/143 (72%), Positives = 125/143 (87%) Frame = +2 Query: 185 ESEDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFXEA 364 E DLY ++K L+R LE L++QE +IK++ ++LK+E + AQEEVKRIQSVPLVIGQF EA Sbjct: 5 EEIDLYNRWKALERQLEMLDLQEGFIKEDCKSLKRELIRAQEEVKRIQSVPLVIGQFLEA 64 Query: 365 VDQNTGIVGSTTGSNYYVRXLSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQ 544 +DQNT IVGSTTGSNY VR LST+DRELLKPSASVAL +HSNALVD+LPPEADSSISML+ Sbjct: 65 IDQNTAIVGSTTGSNYVVRILSTLDRELLKPSASVALQRHSNALVDILPPEADSSISMLR 124 Query: 545 ADEKPDVQYSDIGGMDTQKQEIR 613 DE+PDV Y+D+GG+D QKQE+R Sbjct: 125 PDERPDVSYADVGGLDVQKQEVR 147 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 99 bits (238), Expect = 3e-22 Identities = 56/170 (32%), Positives = 102/170 (60%), Gaps = 7/170 (4%) Frame = +2 Query: 125 TKSQXSKGLAFAGPQSFDELESEDLYTKYK----KLQRMLEFLEVQEEYIKDEQRNLKKE 292 T++ K A +GP + +L + T+ + K+QR+ + L ++EEY+++++R LK + Sbjct: 37 TRTGRRKKKAQSGPDASAKLPTVIPTTRCRLRLLKMQRIHDHLLMEEEYVQNQER-LKPQ 95 Query: 293 YLHAQEEVKRIQSV---PLVIGQFXEAVDQNTGIVGSTTGSNYYVRXLSTIDRELLKPSA 463 QEE R+ + P+ +G E +D + IV ST G YYV +S +D+++L+P Sbjct: 96 DERTQEERNRVDEIRGTPMSVGTLEEIIDDDHAIV-STAGPEYYVSIMSFVDKDMLEPGC 154 Query: 464 SVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIR 613 SV LH + ++V +L + D I++++ D+ P Y+DIGG+++Q QEI+ Sbjct: 155 SVLLHHKAMSIVGLLLDDTDPMINVMKLDKAPTESYADIGGLESQIQEIK 204 >SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizosaccharomyces pombe|chr 3|||Manual Length = 388 Score = 65.7 bits (153), Expect = 5e-12 Identities = 34/143 (23%), Positives = 74/143 (51%) Frame = +2 Query: 185 ESEDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFXEA 364 E + KYK + + + ++ R+L K+Y ++++K +QSV +IG+ + Sbjct: 3 ERDTALEKYKSYLLQHREWDSKLKDLRFGNRDLVKKYDKTEDDIKSLQSVGQIIGEVLKQ 62 Query: 365 VDQNTGIVGSTTGSNYYVRXLSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQ 544 +D IV +++G Y V + +D+ L V+L + ++ +LP E D + + Sbjct: 63 LDSERFIVKASSGPRYVVGCRNNVDQSHLVQGVRVSLDMTTLTIMRILPREVDPLVYNMS 122 Query: 545 ADEKPDVQYSDIGGMDTQKQEIR 613 ++ D+ ++ +GG++ Q +E+R Sbjct: 123 IEDPGDISFAGVGGLNEQIRELR 145 >SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 59.3 bits (137), Expect = 5e-10 Identities = 33/117 (28%), Positives = 60/117 (51%), Gaps = 1/117 (0%) Frame = +2 Query: 266 DEQRN-LKKEYLHAQEEVKRIQSVPLVIGQFXEAVDQNTGIVGSTTGSNYYVRXLSTIDR 442 + QRN L +EE++ +Q +G+ + + +N +V Y V ID Sbjct: 42 EAQRNGLNARVRLLREEIQLLQEPGSYVGEVIKTMGKNKVLVKVHPEGKYVVDISPDIDI 101 Query: 443 ELLKPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIR 613 + +KP+ VAL S L+ +LP + D +S++ ++ PD Y +GG++ Q +EI+ Sbjct: 102 KEIKPNIRVALRNDSYQLIKILPNKVDPLVSLMMVEKIPDSTYEMVGGLEKQIKEIK 158 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 59.3 bits (137), Expect = 5e-10 Identities = 35/161 (21%), Positives = 81/161 (50%), Gaps = 21/161 (13%) Frame = +2 Query: 191 EDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFXEAVD 370 ++L ++ + L+ ++ ++ + + + E+ + ++ QE++ + +P ++G E +D Sbjct: 37 DELNSRTRLLENDIKVMKSEFQRLTHEKSTMLEKIKENQEKISNNKMLPYLVGNVVEILD 96 Query: 371 -------------QNT--------GIVGSTTGSNYYVRXLSTIDRELLKPSASVALHKHS 487 QN+ ++ ++T ++ + ++ E L P + ++K S Sbjct: 97 MQPDEVDVQESANQNSEATRVGKSAVIKTSTRQTIFLPLIGLVEPEELHPGDLIGVNKDS 156 Query: 488 NALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEI 610 ++D LP E DS + ++ DEKP +YSDIGG+ Q +E+ Sbjct: 157 YLIIDKLPSEYDSRVKAMEVDEKPTERYSDIGGLSKQIEEL 197 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 48.8 bits (111), Expect = 7e-07 Identities = 22/85 (25%), Positives = 44/85 (51%) Frame = +2 Query: 359 EAVDQNTGIVGSTTGSNYYVRXLSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISM 538 ++ ++N ++ + + V + ++ V ++ A+ LPP+ D S++M Sbjct: 108 QSAEKNAYVINLKQIAKFVVSLGERVSPTDIEEGMRVGCDRNKYAIQLPLPPKIDPSVTM 167 Query: 539 LQADEKPDVQYSDIGGMDTQKQEIR 613 +Q +EKPDV Y D+GG Q + +R Sbjct: 168 MQVEEKPDVTYGDVGGCKEQIERLR 192 >SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosaccharomyces pombe|chr 2|||Manual Length = 506 Score = 32.7 bits (71), Expect = 0.047 Identities = 14/52 (26%), Positives = 28/52 (53%), Gaps = 1/52 (1%) Frame = -2 Query: 397 GAANYACILINC-LXKLSNDKRNRLYALYFFLCMQIFFLQVSLFIFNVLFLY 245 G Y C+++ + ++N +N+ A FF C+ + F Q++L+ +F Y Sbjct: 182 GHFQYNCVMLGLVMYAIANLLKNQYVAATFFFCLALTFKQMALYFAPPIFFY 233 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 28.7 bits (61), Expect = 0.77 Identities = 11/23 (47%), Positives = 17/23 (73%) Frame = +2 Query: 551 EKPDVQYSDIGGMDTQKQEIRGS 619 E P+V +SDIGG + KQ+++ S Sbjct: 541 ESPNVHWSDIGGQEEVKQKLKES 563 >SPBC336.08 |spc24||spindle pole body protein Spc24|Schizosaccharomyces pombe|chr 2|||Manual Length = 198 Score = 28.7 bits (61), Expect = 0.77 Identities = 21/55 (38%), Positives = 26/55 (47%), Gaps = 2/55 (3%) Frame = +2 Query: 167 QSFDELESEDLYTKYKKLQRMLEFLEVQEEYIKD--EQRNLKKEYLHAQEEVKRI 325 QSF E E E TK+K L R LE E +K E +E L ++E RI Sbjct: 36 QSFREKELEGSQTKFKVLSRKLEISTQAMESLKQTAESSQHAEEILSREKEKFRI 90 >SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 682 Score = 28.3 bits (60), Expect = 1.0 Identities = 12/27 (44%), Positives = 20/27 (74%) Frame = -2 Query: 328 LYALYFFLCMQIFFLQVSLFIFNVLFL 248 +++L+FFLCM+ FF +++ F LFL Sbjct: 360 IFSLFFFLCMRNFFPGFTIY-FPALFL 385 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 27.9 bits (59), Expect = 1.3 Identities = 21/70 (30%), Positives = 32/70 (45%) Frame = +2 Query: 113 RXKMTKSQXSKGLAFAGPQSFDELESEDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKE 292 R K K K L A + E+E L + Q+ LE +E K E++ LK+E Sbjct: 592 REKEKKRDKKKQLKLAKEEERQRREAERLAEQ--AAQKALEAKRQEEARKKREEQRLKRE 649 Query: 293 YLHAQEEVKR 322 Q+E++R Sbjct: 650 QEKKQQELER 659 >SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 608 Score = 27.9 bits (59), Expect = 1.3 Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Frame = +2 Query: 452 KPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQY-SDIGGMDTQKQEI 610 KPS++V+ S+A DV+ + S+I+ D K DV + + D Q +E+ Sbjct: 244 KPSSNVSRGTPSSAHSDVIEISSSSAIARTSGDRKDDVLFDTSTQRRDNQTREM 297 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 27.9 bits (59), Expect = 1.3 Identities = 10/21 (47%), Positives = 17/21 (80%) Frame = +2 Query: 551 EKPDVQYSDIGGMDTQKQEIR 613 E P+V++ DIGG++ K+E+R Sbjct: 490 EVPNVRWEDIGGLEEVKRELR 510 >SPBC1271.10c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 581 Score = 27.5 bits (58), Expect = 1.8 Identities = 19/66 (28%), Positives = 31/66 (46%) Frame = +2 Query: 413 YVRXLSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMD 592 +V L T + +A++ K D L + D + +Q+D KPDV+ SD MD Sbjct: 227 FVLFLFTFEETTFDRAAAID-RKQIQISNDQLTVKEDFDLKDMQSDIKPDVEKSD--AMD 283 Query: 593 TQKQEI 610 K ++ Sbjct: 284 DSKMKV 289 >SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 27.5 bits (58), Expect = 1.8 Identities = 26/123 (21%), Positives = 51/123 (41%) Frame = +2 Query: 191 EDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFXEAVD 370 E+ + QR +E QEE IK+E+ ++E +EEV +S + + + + Sbjct: 333 EEAVKSLPETQRTTSRIETQEEEIKEEEMEGEEE--EEEEEVPNYESENELEDKVGD-LS 389 Query: 371 QNTGIVGSTTGSNYYVRXLSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQAD 550 + G+ S + +R + + E S S+ S +V +P + S++ Sbjct: 390 LSLGVASSFV--DEMLRERNNLSAEGTSASPSLDKKSESTNIVQPIPSHPNDSLNPFYNS 447 Query: 551 EKP 559 P Sbjct: 448 SSP 450 >SPBC32H8.09 |||WD repeat protein, human WDR8 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 483 Score = 27.5 bits (58), Expect = 1.8 Identities = 11/33 (33%), Positives = 15/33 (45%) Frame = -3 Query: 318 FTSSCACKYSFFKFRCSSLMYSSCTSKNSNILW 220 F C S +F C ++ TSK N+LW Sbjct: 313 FPEPSFCSASKIRFNCDETYAATITSKYPNVLW 345 >SPAC30D11.08c |phf2|swp2, saf60|PHD finger containing protein Phf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 538 Score = 26.6 bits (56), Expect = 3.1 Identities = 27/90 (30%), Positives = 42/90 (46%), Gaps = 2/90 (2%) Frame = +2 Query: 185 ESEDLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFXEA 364 ES+ TK+ E +EV ++Y++ E+ EYL QE + V V+ F A Sbjct: 378 ESQSKTTKHSTSLPSTEPVEVDKQYMESEKIPTIDEYL--QEYSNEDEIVLQVLESFPAA 435 Query: 365 VDQNTGIVGSTTGSNYYVRXL--STIDREL 448 V +T + +T + Y R + S I R L Sbjct: 436 VSFST--ITNTIQAKYSNRKIKNSNITRSL 463 >SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 347 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +2 Query: 284 KKEYLHAQEEVKRIQSVPLVIGQFXEAVDQNTGI 385 K ++ A E+ + VPL +F + VDQN G+ Sbjct: 51 KSKHSEAVVEILKSAKVPLKNKEFSDLVDQNIGV 84 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 26.2 bits (55), Expect = 4.1 Identities = 22/70 (31%), Positives = 30/70 (42%) Frame = +2 Query: 368 DQNTGIVGSTTGSNYYVRXLSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQA 547 D+NT + SN + I ELL PS A+ +SN D+ P SS + A Sbjct: 14 DENTDALFKLHTSNRKSANMFGIKSELLNPSELSAVGSYSN---DICPNRQSSSST--AA 68 Query: 548 DEKPDVQYSD 577 D P S+ Sbjct: 69 DTSPSTNASN 78 >SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha subunit Tfg1|Schizosaccharomyces pombe|chr 3|||Manual Length = 490 Score = 25.8 bits (54), Expect = 5.4 Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 2/56 (3%) Frame = +2 Query: 113 RXKMTKSQXSKGLAFAGPQSFDELESED--LYTKYKKLQRMLEFLEVQEEYIKDEQ 274 + K+ K + L Q D E D + + KKLQR L+ LE Y DE+ Sbjct: 291 KDKIKKEMLTANLFGEADQDVDLEEENDRQMSREGKKLQRYLKLLEKNLAYESDEE 346 >SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 779 Score = 25.4 bits (53), Expect = 7.2 Identities = 31/124 (25%), Positives = 48/124 (38%), Gaps = 8/124 (6%) Frame = +2 Query: 245 VQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFXEAVDQN------TGIVGSTTGS 406 + E+ ++ LK E +E++ Q + LV E D N T ++ + S Sbjct: 60 ILEKVVEKVLDTLKAE---VEEKLASSQDLVLVDSDMEEQSDSNLMEVKDTNVINKSITS 116 Query: 407 NYYVRXLSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQADEKP--DVQYSDI 580 + L ID E K S S D S +A+ +P D+ SDI Sbjct: 117 LWSSPNLKEIDGEDEKKSVGQESITGSAKRKDRRSKTNGSKRQKAEANREPPSDISLSDI 176 Query: 581 GGMD 592 GG+D Sbjct: 177 GGLD 180 >SPCC126.14 |prp18||U5 snRNP-associated protein Prp18|Schizosaccharomyces pombe|chr 3|||Manual Length = 343 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/25 (52%), Positives = 17/25 (68%) Frame = +2 Query: 245 VQEEYIKDEQRNLKKEYLHAQEEVK 319 +QE+ KDEQR LKK L +E +K Sbjct: 41 LQEKQQKDEQRELKKRKLE-EERLK 64 >SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1953 Score = 25.0 bits (52), Expect = 9.5 Identities = 31/145 (21%), Positives = 55/145 (37%), Gaps = 2/145 (1%) Frame = +2 Query: 146 GLAFAGPQSFD-ELESEDLYTKY-KKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVK 319 G G S D ++ + T Y ++L + L++ EY+ D N + + + + Sbjct: 190 GKKLLGSASRDYDVNPANYSTHYLQQLSNLKSRLDLAGEYLDDSIMNDLGDNVGSNSKGS 249 Query: 320 RIQSVPLVIGQFXEAVDQNTGIVGSTTGSNYYVRXLSTIDRELLKPSASVALHKHSNALV 499 S+P + +++ N T N + LS R LK A + ++ Sbjct: 250 PTTSIP----EHKTSINNNKPEDTPTPSENVH---LSARQRNALKRKARQMKNSQKVRVI 302 Query: 500 DVLPPEADSSISMLQADEKPDVQYS 574 DV P S AD+K Y+ Sbjct: 303 DVAPTLVHQQNSTSSADKKTGADYN 327 >SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/31 (38%), Positives = 20/31 (64%) Frame = +2 Query: 209 YKKLQRMLEFLEVQEEYIKDEQRNLKKEYLH 301 Y+++QR+LE LE++E D L+ Y+H Sbjct: 95 YQQIQRLLEKLEIKEGNYGDGYA-LQPSYIH 124 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,338,530 Number of Sequences: 5004 Number of extensions: 42001 Number of successful extensions: 170 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 165 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 169 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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