Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0978
         (700 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g10760.1 68417.m01756 methyltransferase MT-A70, putative simi...   163   1e-40
At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEME...    31   0.56 
At5g18780.1 68418.m02231 F-box family protein contains F-box dom...    30   1.3  
At3g09180.1 68416.m01090 expressed protein                             30   1.7  
At4g33540.1 68417.m04767 metallo-beta-lactamase family protein         29   3.0  
At4g20160.1 68417.m02949 expressed protein ; expression supporte...    29   3.0  
At4g09980.1 68417.m01634 methyltransferase MT-A70 family protein...    29   3.9  
At2g45400.1 68415.m05646 dihydroflavonol 4-reductase family / di...    29   3.9  
At5g09840.1 68418.m01138 expressed protein contains Pfam profile...    28   5.2  
At2g32000.1 68415.m03910 DNA topoisomerase family protein simila...    28   5.2  
At5g39770.1 68418.m04817 repair endonuclease family protein cont...    28   6.8  
At3g01270.1 68416.m00033 pectate lyase family protein similar to...    27   9.0  

>At4g10760.1 68417.m01756 methyltransferase MT-A70, putative similar
           to  (N6-adenosine)-methyltransferase [Mus musculus]
           GI:10179948, m6A methyltransferase (MT-A70) [Homo
           sapiens] GI:2460037; contains Pfam profile PF05063:
           MT-A70 (S-adenosylmethionine-binding subunit of human
           mRNA:m6A methyl-transferase (MTase))
          Length = 685

 Score =  163 bits (396), Expect = 1e-40
 Identities = 86/199 (43%), Positives = 114/199 (57%), Gaps = 2/199 (1%)
 Frame = +1

Query: 52  RSLARATQXIMSLLAMPSSREKAVKRVGEEIMDLLSKPTAKERSLADKFKSQGGAQVMEF 231
           RS     + + +LL+  S +EK   R GEE++DL+ +PTAKE + A KFKS+GG+QV  +
Sbjct: 311 RSEEEDLKDVEALLSKKSFKEKQQSRTGEELLDLIHRPTAKEAATAAKFKSKGGSQVKYY 370

Query: 232 CPHGTRAECGRAISPDSAQDGTTNTCKKLHFKKIIQGHTDETLGDCSFLNTCFHMDSCKY 411
           C + T+ +C         Q G+   C K HF+++I  HTD +LGDCSFL+TC HM +CKY
Sbjct: 371 CRYLTKEDC-------RLQSGSHIACNKRHFRRLIASHTDVSLGDCSFLDTCRHMKTCKY 423

Query: 412 VHYEVDNTDPNANTKTVEALTKSVQNGTSSISKCLNTD--GVLTLTPPQWIQCDLRYLDM 585
           VHYE+D  D             ++  G     K L  D      L   QWI CD+R   M
Sbjct: 424 VHYELDMAD-------------AMMAGPDKALKPLRADYCSEAELGEAQWINCDIRSFRM 470

Query: 586 TFLGKFAVIMADPPWDIHM 642
             LG F V+MADPPWDIHM
Sbjct: 471 DILGTFGVVMADPPWDIHM 489



 Score = 36.3 bits (80), Expect = 0.020
 Identities = 14/18 (77%), Positives = 16/18 (88%)
 Frame = +2

Query: 644 ELPYGTMSDDEMRCLGIP 697
           ELPYGTM+DDEMR L +P
Sbjct: 490 ELPYGTMADDEMRTLNVP 507


>At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEMETER
            protein [Arabidopsis thaliana] GI:21743571; contains Pfam
            profile PF00730: HhH-GPD superfamily base excision DNA
            repair protein
          Length = 1729

 Score = 31.5 bits (68), Expect = 0.56
 Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 6/85 (7%)
 Frame = +1

Query: 271  SPDSAQDGTTNTCKKLHF-KKIIQGHTDETLG-----DCSFLNTCFHMDSCKYVHYEVDN 432
            S +  +D + +  ++ +F +K IQ   +E L      D +   +C  + SC     + + 
Sbjct: 849  SKEQLRDCSNSGIERFNFLEKSIQNLEEEVLSSQDSFDPAIFQSCGRVGSCSCSKSDAEF 908

Query: 433  TDPNANTKTVEALTKSVQNGTSSIS 507
                  TKTV   ++SVQ G+ ++S
Sbjct: 909  PTTRCETKTVSGTSQSVQTGSPNLS 933


>At5g18780.1 68418.m02231 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 441

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 12/31 (38%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
 Frame = +1

Query: 343 HTDETLGDCSFLNTCF-HMDSCKYVHYEVDN 432
           +TD  + D S L+ C   +D CK  H+E++N
Sbjct: 88  NTDHDVYDISTLDACLMRLDKCKIQHFEIEN 118


>At3g09180.1 68416.m01090 expressed protein
          Length = 402

 Score = 29.9 bits (64), Expect = 1.7
 Identities = 16/49 (32%), Positives = 22/49 (44%)
 Frame = -1

Query: 286 VHYRG**LYHIQLWCRADRTPLLEHLLGF*ICQLRTSL*LWACSINPLF 140
           +H RG  +YH+           L++ LGF       SL LW CS   +F
Sbjct: 302 LHARGFSVYHVYKHITEHAATALQYFLGFGTGTALYSLLLWICSFESVF 350


>At4g33540.1 68417.m04767 metallo-beta-lactamase family protein
          Length = 355

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 18/69 (26%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
 Frame = +1

Query: 346 TDETLGDCSFLNTCFHMDSCKYVHYEVDNTDPNANTKTVEALTKSVQ-NGTSSISKCLNT 522
           T    GD    NTC   D+C+++  ++     N +  T +   K  + N   ++  C  T
Sbjct: 59  TQNVEGDIFVDNTCIDCDTCRWMVPDLFTRVDNMSAVTKQPTCKEERLNALQALLSC-PT 117

Query: 523 DGVLTLTPP 549
             + T TPP
Sbjct: 118 GSIRTQTPP 126


>At4g20160.1 68417.m02949 expressed protein ; expression supported by
            MPSS
          Length = 1188

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 26/82 (31%), Positives = 38/82 (46%)
 Frame = +1

Query: 109  REKAVKRVGEEIMDLLSKPTAKERSLADKFKSQGGAQVMEFCPHGTRAECGRAISPDSAQ 288
            +E+   + GE+ M  L    AK     ++ KSQ  +Q  E   HG+R       S  S Q
Sbjct: 811  KERNSLQYGEK-MCFLRNSEAKSTKEIERNKSQEVSQGEESASHGSRESAKEKNS--SQQ 867

Query: 289  DGTTNTCKKLHFKKIIQGHTDE 354
            D  T+T +  + KK I+   DE
Sbjct: 868  DDETSTHRNPNDKKGIKEPEDE 889


>At4g09980.1 68417.m01634 methyltransferase MT-A70 family protein
           low similarity to SP|P25583 Karyogamy protein KAR4
           {Saccharomyces cerevisiae},
           (N6-adenosine)-methyltransferase [Mus musculus]
           GI:10179948; contains Pfam profile PF05063: MT-A70
           (S-adenosylmethionine-binding subunit of human mRNA:m6A
           methyl-transferase (MTase))
          Length = 775

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 13/38 (34%), Positives = 24/38 (63%), Gaps = 3/38 (7%)
 Frame = +1

Query: 547 PQWIQCDLRYLDMT---FLGKFAVIMADPPWDIHMGAA 651
           P +++ DL  ++++   F  KF VI+ DPPW+ ++  A
Sbjct: 617 PMYLKGDLHEVELSPELFGTKFDVILVDPPWEEYVHRA 654


>At2g45400.1 68415.m05646 dihydroflavonol 4-reductase family /
           dihydrokaempferol 4-reductase family similar to
           dihydroflavonol 4-reductase (SP:P51102), vestitone
           reductase (Medicago sativa, GI:973249)
          Length = 364

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 17/47 (36%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
 Frame = +1

Query: 394 MDSCKYVHYEVDNTDPNANTKTVEALTKSVQNGTSSISK-CLNTDGV 531
           ++ CK V +     DPN+N +T E +TK    G   I K CL+   V
Sbjct: 109 IEGCKAVFHVAHPMDPNSN-ETEETVTKRTVQGLMGILKSCLDAKTV 154


>At5g09840.1 68418.m01138 expressed protein contains Pfam profile
           PF04396: Protein of unknown function, DUF537
          Length = 924

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 15/46 (32%), Positives = 24/46 (52%)
 Frame = +1

Query: 409 YVHYEVDNTDPNANTKTVEALTKSVQNGTSSISKCLNTDGVLTLTP 546
           Y HY +   DP A   T E  + SV+     +S+ +N++ V+ L P
Sbjct: 239 YGHYRIPLLDPFAIATTTEQSSSSVK--IEELSESVNSNAVVNLRP 282


>At2g32000.1 68415.m03910 DNA topoisomerase family protein similar
           to DNA topoisomerase III beta-1 (EC 5.99.1.2)(SP:Q9Z321)
           {Mus musculus}
          Length = 865

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 12/43 (27%), Positives = 21/43 (48%)
 Frame = -1

Query: 523 LYSSTSISNLYHFAQTSSAPPLS*CSHLDRCYPLRNERTCSCP 395
           L+ ++   N     +T +  P S C H    + +RN+  C+CP
Sbjct: 698 LFGASKTPNAPAKTKTGAGMPCSLCPHPTCQHSVRNQGVCACP 740


>At5g39770.1 68418.m04817 repair endonuclease family protein
           contains Pfam PF02732 : ERCC4 domain; similar to MUS81
           endonuclease (GI:16755674) [Mus musculus]; similar to
           repair endonuclease (TIGR:At5g41150) [Arabidopsis
           thaliana]
          Length = 1242

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 15/51 (29%), Positives = 23/51 (45%)
 Frame = -1

Query: 583 CPNI*GRTVSTAEASESKHRLYSSTSISNLYHFAQTSSAPPLS*CSHLDRC 431
           CP   G      +  + KH LY   + +N+  F + SS P L+  S   +C
Sbjct: 2   CPKNQGLADYVLQKKQEKHCLYWGCNNNNIRDFGRLSSEPRLNRKSRKTKC 52


>At3g01270.1 68416.m00033 pectate lyase family protein similar to
           pectate lyase P59 SP:P15722 from [Lycopersicon
           esculentum]
          Length = 475

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
 Frame = +1

Query: 103 SSREKAVKRVGEEIMDLLSKPT-AKERSLADKFKSQG 210
           +  EKAV    E++++++S PT +  RSL  + K +G
Sbjct: 74  TEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKG 110


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,735,930
Number of Sequences: 28952
Number of extensions: 335148
Number of successful extensions: 956
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 912
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 954
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)