Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0966
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 341 5e-95
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 341 7e-95
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 341 7e-95
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 123 3e-29
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 120 2e-28
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 83 3e-17
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 33 0.057
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 28 1.2
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 27 2.9
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 2.9
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.8
SPAPB1A11.04c |||transcription factor |Schizosaccharomyces pombe... 27 3.8
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 5.0
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 26 6.6
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 8.7
SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 25 8.7
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 341 bits (839), Expect = 5e-95
Identities = 161/236 (68%), Positives = 188/236 (79%)
Frame = +1
Query: 7 TSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEP 186
TSQADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+
Sbjct: 106 TSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQA 163
Query: 187 RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADG 366
RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G G
Sbjct: 164 RFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKG 223
Query: 367 KCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANI 546
K L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +
Sbjct: 224 KTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGV 283
Query: 547 TTEVKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRSWVMLAGDSKNNPP*GCCRF 714
TTEVKSVEMHHE+L +PGD ++R + GDSKN+PP GC F
Sbjct: 284 TTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRG-NVCGDSKNDPPMGCASF 338
Score = 27.1 bits (57), Expect = 2.9
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = +2
Query: 701 GAADFTAQVIGLNHXG 748
G A FTAQVI LNH G
Sbjct: 334 GCASFTAQVIILNHPG 349
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 341 bits (838), Expect = 7e-95
Identities = 160/236 (67%), Positives = 188/236 (79%)
Frame = +1
Query: 7 TSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEP 186
TSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+
Sbjct: 106 TSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQA 163
Query: 187 RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADG 366
RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G G
Sbjct: 164 RFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKG 223
Query: 367 KCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANI 546
K L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +
Sbjct: 224 KTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGV 283
Query: 547 TTEVKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRSWVMLAGDSKNNPP*GCCRF 714
TTEVKSVEMHHE+L +PGD ++R + GDSKN+PP GC F
Sbjct: 284 TTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRG-NVCGDSKNDPPMGCASF 338
Score = 27.1 bits (57), Expect = 2.9
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = +2
Query: 701 GAADFTAQVIGLNHXG 748
G A FTAQVI LNH G
Sbjct: 334 GCASFTAQVIILNHPG 349
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 341 bits (838), Expect = 7e-95
Identities = 160/236 (67%), Positives = 188/236 (79%)
Frame = +1
Query: 7 TSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEP 186
TSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+
Sbjct: 106 TSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTG--WSQA 163
Query: 187 RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADG 366
RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G G
Sbjct: 164 RFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKG 223
Query: 367 KCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANI 546
K L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +
Sbjct: 224 KTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGV 283
Query: 547 TTEVKSVEMHHEALQEAVPGDXXXXXXXXXXXXELRSWVMLAGDSKNNPP*GCCRF 714
TTEVKSVEMHHE+L +PGD ++R + GDSKN+PP GC F
Sbjct: 284 TTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRG-NVCGDSKNDPPMGCASF 338
Score = 27.1 bits (57), Expect = 2.9
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = +2
Query: 701 GAADFTAQVIGLNHXG 748
G A FTAQVI LNH G
Sbjct: 334 GCASFTAQVIILNHPG 349
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 123 bits (296), Expect = 3e-29
Identities = 71/205 (34%), Positives = 117/205 (57%), Gaps = 5/205 (2%)
Frame = +1
Query: 10 SQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPR 189
SQAD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +NKMD +SE R
Sbjct: 338 SQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEER 397
Query: 190 FEEIKKEVSSYIKKI-GYNPAA-VAFVPISGWHGDNMLE--PSTKMPWFKGWQVERKEGK 357
++E ++S +++++ GYN V ++P+S + G N+ + S+ PW++
Sbjct: 398 YKECVDKLSMFLRRVAGYNSKTDVKYMPVSAYTGQNVKDRVDSSVCPWYQ---------- 447
Query: 358 ADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAP 537
G L+E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P
Sbjct: 448 --GPSLLEYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMP 503
Query: 538 ANITTEVKSV-EMHHEALQEAVPGD 609
N T EV ++ + E + ++ GD
Sbjct: 504 INQTLEVTAIYDEADEEISSSICGD 528
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 120 bits (289), Expect = 2e-28
Identities = 79/204 (38%), Positives = 111/204 (54%), Gaps = 4/204 (1%)
Frame = +1
Query: 10 SQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPR 189
S AD AVL+V + FE G +NGQTREHA L LG+ +++V VNK+D +SE R
Sbjct: 277 SSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDR 334
Query: 190 FEEIKKEVSSY-IKKIGYNPAAVAFVPISGWHGDNML--EPSTKMPWFKGWQVERKEGKA 360
F+EIK VS + IK +G+ + V FVPIS G N++ + S W+K
Sbjct: 335 FQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYK----------- 383
Query: 361 DGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPA 540
G L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G ++ ++ +
Sbjct: 384 -GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSS 441
Query: 541 NITTEVKSVEMHHEALQE-AVPGD 609
VK+V + + AV GD
Sbjct: 442 QEDAYVKNVIRNSDPSSTWAVAGD 465
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 83.4 bits (197), Expect = 3e-17
Identities = 68/201 (33%), Positives = 102/201 (50%), Gaps = 4/201 (1%)
Frame = +1
Query: 19 DCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEE 198
D A+++V+A G+ QTREH LLA +GVKQ++V +NK+D EP E E
Sbjct: 141 DGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEME 193
Query: 199 IKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM-LEPSTKMPWFKGWQVERKEGKADGKCL 375
+++ +S Y G+ GDN + + + +G + E G L
Sbjct: 194 MRELLSEY-----------------GFDGDNTPIVSGSALCALEGREPEI--GLNSITKL 234
Query: 376 IEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPANI 546
+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G +V +++
Sbjct: 235 MEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHL 294
Query: 547 TTEVKSVEMHHEALQEAVPGD 609
T V +EM + L AV GD
Sbjct: 295 KTTVTGIEMFKKQLDAAVAGD 315
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 32.7 bits (71), Expect = 0.057
Identities = 26/94 (27%), Positives = 40/94 (42%)
Frame = +1
Query: 313 MPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRV 492
+P K V K GK + L E + I PP + PLR L D + G + + R+
Sbjct: 206 IPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGVIGLVRI 265
Query: 493 ETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE 594
G +K G V+ EV+ V + + + E
Sbjct: 266 MEGFIKKGGKVMSVNTGRKYEVQQVGIMYPDMTE 299
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 28.3 bits (60), Expect = 1.2
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -2
Query: 413 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 276
G GG S+ P+ LPS + P L E +ML CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 27.1 bits (57), Expect = 2.9
Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = +2
Query: 176 TVSPDLRKSRRKYPHTSRRLATTQLL---SLSCPFLDGTETTCWSLQPKCLGSRDGRWSV 346
+ +PD+ ++PHT +R +T + L LSC + +E + P G +D + +
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRCLSCEYSTLSEASDSMSNPLFFGDQDNGYVI 204
Query: 347 RKA 355
++
Sbjct: 205 SQS 207
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 2.9
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = -2
Query: 557 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 441
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1
Query: 651 QFLDGHVLYVETYIVSRYSFLESFV 577
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAPB1A11.04c |||transcription factor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 697
Score = 26.6 bits (56), Expect = 3.8
Identities = 9/18 (50%), Positives = 11/18 (61%)
Frame = -3
Query: 721 SCKICSTLKVGCFWSHQP 668
SC+ CS KV C W +P
Sbjct: 40 SCQACSNNKVECIWPDRP 57
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 5.0
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = -2
Query: 515 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 351
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 350 SLRSTCHPLNQG 315
P G
Sbjct: 374 PADGVPKPTPDG 385
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 25.8 bits (54), Expect = 6.6
Identities = 17/67 (25%), Positives = 31/67 (46%)
Frame = -1
Query: 543 VGGGKDNNGTWFQHTSFNSADGHGTNTTDFVYVLQGKTQGLVSGAGRWQDGIESFNEAFS 364
VG D+ + + + + DF+ ++GK L + R+++ ES NE
Sbjct: 125 VGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNESTNERVK 184
Query: 363 VSFAFLT 343
+FA+LT
Sbjct: 185 -NFAYLT 190
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 8.7
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2
Query: 506 NTPVSTLPTGTVPIPPIL 453
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 25.4 bits (53), Expect = 8.7
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = +1
Query: 154 MDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWF 324
+D +P + F IKK+V Y+ ++ + P + G N LEP TK PW+
Sbjct: 107 LDLKKPLLPQILFGNIKKDV--YLDQV-HRPRHYRGSGSAPLFG-NFLEPLTKTPWY 159
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,266,648
Number of Sequences: 5004
Number of extensions: 71518
Number of successful extensions: 265
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 255
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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