Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0928
(450 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 202 9e-53
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 202 9e-53
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 202 9e-53
At1g29760.1 68414.m03639 expressed protein 31 0.27
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 1.5
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 28 2.5
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 28 2.5
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 28 2.5
At1g38131.1 68414.m04669 expressed protein contains Pfam PF03138... 27 4.4
At1g01600.1 68414.m00077 cytochrome P450, putative similar to cy... 27 4.4
At1g23880.1 68414.m03012 NHL repeat-containing protein contains ... 27 5.9
At1g12240.1 68414.m01416 beta-fructosidase (BFRUCT4) / beta-fruc... 27 5.9
At5g20720.2 68418.m02461 20 kDa chaperonin, chloroplast (CPN21) ... 27 7.7
At5g20720.1 68418.m02460 20 kDa chaperonin, chloroplast (CPN21) ... 27 7.7
At5g10730.1 68418.m01243 expressed protein 27 7.7
At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family prot... 27 7.7
At1g78040.1 68414.m09094 pollen Ole e 1 allergen and extensin fa... 27 7.7
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 202 bits (493), Expect = 9e-53
Identities = 95/141 (67%), Positives = 108/141 (76%)
Frame = +2
Query: 23 PKHGCWTNLAVCMHRVPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDG 202
PKH L PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+VDG
Sbjct: 15 PKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDG 74
Query: 203 KVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 382
KVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K
Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134
Query: 383 NVPYLVTHDGRTIRYPDPLIK 445
+PYL T+DGRTIRYPDPLIK
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIK 155
Score = 48.0 bits (109), Expect = 3e-06
Identities = 22/37 (59%), Positives = 25/37 (67%)
Frame = +1
Query: 1 HLKRLNAPKAWMLDKLGGVYAPSTVNRSPQVARVLAP 111
HLKRLNAPK WMLDKLGG +AP + P +R P
Sbjct: 8 HLKRLNAPKHWMLDKLGGAFAPKP-SSGPHKSRECLP 43
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 202 bits (493), Expect = 9e-53
Identities = 95/141 (67%), Positives = 108/141 (76%)
Frame = +2
Query: 23 PKHGCWTNLAVCMHRVPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDG 202
PKH L PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+VDG
Sbjct: 15 PKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDG 74
Query: 203 KVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 382
KVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K
Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134
Query: 383 NVPYLVTHDGRTIRYPDPLIK 445
+PYL T+DGRTIRYPDPLIK
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIK 155
Score = 48.0 bits (109), Expect = 3e-06
Identities = 22/37 (59%), Positives = 25/37 (67%)
Frame = +1
Query: 1 HLKRLNAPKAWMLDKLGGVYAPSTVNRSPQVARVLAP 111
HLKRLNAPK WMLDKLGG +AP + P +R P
Sbjct: 8 HLKRLNAPKHWMLDKLGGAFAPKP-SSGPHKSRECLP 43
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 202 bits (493), Expect = 9e-53
Identities = 95/141 (67%), Positives = 108/141 (76%)
Frame = +2
Query: 23 PKHGCWTNLAVCMHRVPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDG 202
PKH L PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+VDG
Sbjct: 15 PKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDG 74
Query: 203 KVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 382
KVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K
Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134
Query: 383 NVPYLVTHDGRTIRYPDPLIK 445
+PYL T+DGRTIRYPDPLIK
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIK 155
Score = 48.0 bits (109), Expect = 3e-06
Identities = 22/37 (59%), Positives = 25/37 (67%)
Frame = +1
Query: 1 HLKRLNAPKAWMLDKLGGVYAPSTVNRSPQVARVLAP 111
HLKRLNAPK WMLDKLGG +AP + P +R P
Sbjct: 8 HLKRLNAPKHWMLDKLGGAFAPKP-SSGPHKSRECLP 43
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.5 bits (68), Expect = 0.27
Identities = 22/66 (33%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
Frame = -1
Query: 216 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGPVDGTRCIHTAKFVQ 37
S+LTFP +R CF F F + RRFL G S ++G ++ R T + +
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFLMARVAG-ISDMIFGYMNPFRLKDTKQMLS 253
Query: 36 HPC-FG 22
C FG
Sbjct: 254 IVCKFG 259
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 1.5
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -1
Query: 195 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 79
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 2.5
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 128 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 262
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 2.5
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 128 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 262
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 28.3 bits (60), Expect = 2.5
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 128 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 262
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 749
>At1g38131.1 68414.m04669 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'growth regulator -related' based on similarity to axi
1 protein (GB:X80301) (GI:559920) from [Nicotiana
tabacum], which, due to scienitific fraud was retracted.
Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908.
PMID:10400497.
Length = 589
Score = 27.5 bits (58), Expect = 4.4
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1
Query: 46 LGGVYAPSTVNRSPQVARVLAPGDFPEESS 135
+G VY P +V RSP++ R L F + S+
Sbjct: 85 VGWVYVPGSVYRSPEIYRKLKDDIFSDNST 114
>At1g01600.1 68414.m00077 cytochrome P450, putative similar to
cytochrome P450 GI:10442763 from [Triticum aestivum]
Length = 554
Score = 27.5 bits (58), Expect = 4.4
Identities = 12/44 (27%), Positives = 24/44 (54%)
Frame = +2
Query: 62 HRVPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIK 193
HR+ HK+ + + L +F++N L L ++ I+K ++K
Sbjct: 482 HRLTVVPGHKVEQKMSLTLFMKNGLLVNLYKRDLQGIIKSLVVK 525
>At1g23880.1 68414.m03012 NHL repeat-containing protein contains
Pfam profile PF01436: NHL repeat
Length = 545
Score = 27.1 bits (57), Expect = 5.9
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +1
Query: 10 RLNAPKAWMLDKLGGVYAPSTVNRS 84
RLN PK +D G +Y TVN +
Sbjct: 203 RLNNPKGLTVDDRGNIYVADTVNNA 227
>At1g12240.1 68414.m01416 beta-fructosidase (BFRUCT4) /
beta-fructofuranosidase / invertase, vacuolar identical
to beta-fructosidase GI:1871503 from [Arabidopsis
thaliana]; contains Pfam profile PF00251:Glycosyl
hydrolases family 32; identical to cDNA
beta-fructosidase (vacuolar form) GI:1321683; similar to
SP:Q43857
Length = 664
Score = 27.1 bits (57), Expect = 5.9
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = +2
Query: 38 WTNLAVCMHRVPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVK 178
+ L +HRVP+TG + + P+ N L ++ G +V IVK
Sbjct: 296 YEKLDTLLHRVPNTGMWECVDFYPVSKTAGNGLDTSVNGPDVKHIVK 342
>At5g20720.2 68418.m02461 20 kDa chaperonin, chloroplast (CPN21)
(CHCPN10) (CPN20) identical to chloroplast 20 kDa
chaperonin, chloroplast precursor (Protein Cpn21),
chloroplast protein Cpn10, chloroplast chaperonin 10
(Ch-CPN10), SP|O65282 from [Arabidopsis thaliana];
identical to cDNA chaperonin 20 GI:14587372
Length = 253
Score = 26.6 bits (56), Expect = 7.7
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +1
Query: 46 LGGVYAPSTVNRSPQVARVLAPGD 117
LGG+ PST PQ V+A G+
Sbjct: 81 LGGILLPSTAQSKPQGGEVVAVGE 104
>At5g20720.1 68418.m02460 20 kDa chaperonin, chloroplast (CPN21)
(CHCPN10) (CPN20) identical to chloroplast 20 kDa
chaperonin, chloroplast precursor (Protein Cpn21),
chloroplast protein Cpn10, chloroplast chaperonin 10
(Ch-CPN10), SP|O65282 from [Arabidopsis thaliana];
identical to cDNA chaperonin 20 GI:14587372
Length = 253
Score = 26.6 bits (56), Expect = 7.7
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +1
Query: 46 LGGVYAPSTVNRSPQVARVLAPGD 117
LGG+ PST PQ V+A G+
Sbjct: 81 LGGILLPSTAQSKPQGGEVVAVGE 104
>At5g10730.1 68418.m01243 expressed protein
Length = 287
Score = 26.6 bits (56), Expect = 7.7
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = +2
Query: 179 QRLIKVDGKVRTDPTYPAGFMDVVSIEKTNE 271
+ + KV + TDP +P G +DV I++ ++
Sbjct: 253 ESVAKVAVRAATDPVFPPGIVDVHGIQRYSQ 283
>At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family protein
similar to lipase [Arabidopsis thaliana] GI:1145627;
contains Pfam profile:PF00657 Lipase/Acylhydrolase with
GDSL-like motif
Length = 323
Score = 26.6 bits (56), Expect = 7.7
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = -1
Query: 201 PSTFMRRCFTIFRTSFPVK 145
P F CF I+ TSFPVK
Sbjct: 175 PGNFPVGCFPIYLTSFPVK 193
>At1g78040.1 68414.m09094 pollen Ole e 1 allergen and extensin
family protein contains Pfam domain, PF01190: Pollen
proteins Ole e I family
Length = 171
Score = 26.6 bits (56), Expect = 7.7
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -2
Query: 131 DSSGKSPGASTRATCGDRLTVLGAYTPPSLSN 36
+SS PGA+ + +C DR T+ YT ++S+
Sbjct: 49 ESSYFIPGATVKLSCKDRKTMEEVYTDKAVSD 80
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,665,882
Number of Sequences: 28952
Number of extensions: 217981
Number of successful extensions: 627
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 602
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 627
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 732537840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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